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The map label for this gene is def [C]

Identifier: 209549461

GI number: 209549461

Start: 1914545

End: 1915039

Strand: Direct

Name: def [C]

Synonym: Rleg2_1867

Alternate gene names: 209549461

Gene position: 1914545-1915039 (Clockwise)

Preceding gene: 209549458

Following gene: 209549462

Centisome position: 42.19

GC content: 64.65

Gene sequence:

>495_bases
ATGCCCATTCGCCCGATCCTGCGTTACCCGCATCCTGGTCTGAAGACCGTCTGCGCGCCGGTGACCGTCTTCGATTCATC
GCTTGCCGCACTTGCCGACGACCTGCTGGCGACGATGCGCGCCGCGCCCGGCGTCGGCATCACCGCCGCGCATATCGGCG
TTTTCACCCGCCTGACGGTGCTGGAGCTCGACAAGACCGACGGCGTGCGCCTCTACGTCAACCCGGAAATCACCTGGTTC
TCGAAGGAGACGATGAGCCATGCCGAAGGCAGCGTCTCGATGCCGGGAGCAACCGACGAGGTCACGCGCCCGCGGTCGAT
CCGCTTCCGCTATCAGGACGCCGAGGGCGGCATGCACGAGGACGTCGCCGACGATTTCCTCGCCATCTGCATCCAGCACG
AAGTCGACCAGCTCAATGGCATTTTCTGGCTGCAGCGCCTCTCGCGGCTGAAGCGCGACCGCCTCGTGAAGAAATGGGAG
AAGGCGCCAAGTTGA

Upstream 100 bases:

>100_bases
GAAAGCATCCCTGCCGTAAAGCAGCAAACCTGTCGCTTGCTGACAATGCCCGCGGCCGGCGATAGTTTCGCCACGCATCC
GAGCCGAGGAATACCGCCCC

Downstream 100 bases:

>100_bases
CAATCAAGCACTGACGATCACGCCTGACAAATCGGCTCCCGCGCCGAACCATTCGCCGCTGCCGGCGTTTCCAGCAGAGA
AACCCAGCGTTTCCCCCCAA

Product: peptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MPIRPILRYPHPGLKTVCAPVTVFDSSLAALADDLLATMRAAPGVGITAAHIGVFTRLTVLELDKTDGVRLYVNPEITWF
SKETMSHAEGSVSMPGATDEVTRPRSIRFRYQDAEGGMHEDVADDFLAICIQHEVDQLNGIFWLQRLSRLKRDRLVKKWE
KAPS

Sequences:

>Translated_164_residues
MPIRPILRYPHPGLKTVCAPVTVFDSSLAALADDLLATMRAAPGVGITAAHIGVFTRLTVLELDKTDGVRLYVNPEITWF
SKETMSHAEGSVSMPGATDEVTRPRSIRFRYQDAEGGMHEDVADDFLAICIQHEVDQLNGIFWLQRLSRLKRDRLVKKWE
KAPS
>Mature_163_residues
PIRPILRYPHPGLKTVCAPVTVFDSSLAALADDLLATMRAAPGVGITAAHIGVFTRLTVLELDKTDGVRLYVNPEITWFS
KETMSHAEGSVSMPGATDEVTRPRSIRFRYQDAEGGMHEDVADDFLAICIQHEVDQLNGIFWLQRLSRLKRDRLVKKWEK
APS

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=34.5679012345679, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 18330; Mature: 18199

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIRPILRYPHPGLKTVCAPVTVFDSSLAALADDLLATMRAAPGVGITAAHIGVFTRLTV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEE
LELDKTDGVRLYVNPEITWFSKETMSHAEGSVSMPGATDEVTRPRSIRFRYQDAEGGMHE
EEECCCCCEEEEECCCEEEECHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCH
DVADDFLAICIQHEVDQLNGIFWLQRLSRLKRDRLVKKWEKAPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIRPILRYPHPGLKTVCAPVTVFDSSLAALADDLLATMRAAPGVGITAAHIGVFTRLTV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEE
LELDKTDGVRLYVNPEITWFSKETMSHAEGSVSMPGATDEVTRPRSIRFRYQDAEGGMHE
EEECCCCCEEEEECCCEEEECHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCH
DVADDFLAICIQHEVDQLNGIFWLQRLSRLKRDRLVKKWEKAPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]