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The map label for this gene is macA [H]

Identifier: 209549302

GI number: 209549302

Start: 1737340

End: 1738632

Strand: Direct

Name: macA [H]

Synonym: Rleg2_1706

Alternate gene names: 209549302

Gene position: 1737340-1738632 (Clockwise)

Preceding gene: 209549301

Following gene: 209549303

Centisome position: 38.28

GC content: 64.5

Gene sequence:

>1293_bases
ATGAACGACACCGGAACCGGCAAGAAGACCGGGCCACAGACAAGCGGCGCGTCCGATCTCGCGGCGGTGCTTGCCGCATC
GGGACGTCAGGGCAAGCGCAGCCGCTGGCGTGGACGCCTGTTCATCCTGCTGATCCTCGTCGCTGCTGCCGCCGGCGCCG
CCTATTTCTATATGGGCCGCGGGCAAAGCGAAGTGAGCTATGCCACTCAGCCGGCAAAACGGGGAGATCTGACGGTGCTC
GTCACCGCTACCGGCTCGGTGCAGCCGACCGAGCAGGTGGATATATCGAGCGAGCTTTCCGGTACGGTCCGCGACGTCAA
TGTCGATTACAACAGCACGATCAAAGCCGGCGACGTGCTGGCGCTTCTCGATACCAACAAGCTGGAAGCGGATGTGAAGA
GTTCGCGCGCCAAGCTGAATTCTGCCAAGGCGAACGTCGCCAAGGCCAATGCCGATCTGGAATCGGCAAGCACCTCGCTC
GAGCGGCTGAAGAGCCTGGTCAAGAGCAACGTCTCGACCCAGCAGAGCCTCGACGACGCCACCTACAAATATGATTCCGC
CGTCGCCGCCAAACAGATCAACGAAGCCGAGGTGCTCGCCTCGGAGGCCGACCTGCAGCTTGCCGAGGTCAATCTCGCCA
AAGCGAAGATCGTCTCGCCGATCGACGGCGTGATCCTCACCCGCGCCGTCAATCCGGGTGCCACGGTCGCAGCCTCGCTT
TCGGCGCCGATCCTCTTCACCATCGCCGGCGACCTGAAGAAGATGGAGCTGCAGGTCGATGTCGATGAGGCTGATGTCGG
CCAGATTGCCGTCGGCCAGAAGGCGAAGTTCACGGTCGACGCCTATCCCGACCGCAGCTTTCCCGCCGAGATCGAGCAGA
TCCGTTTTGCCTCCGAAGTGGTCAACAATGTCGTGACCTACAAGGCGGTGCTCTCGGTCGACAACGCCGATCTCCTGCTG
CGCCCCGGCATGACGGCGACGGCCGACGTCACCGTCGAAGCCGTCAAGGATACGCTGATGGTGCCGAACGCCGCGCTGCG
CTACGCGCCGGCGCAGGCGGGACGGCGCGGCCGCGGCATTTTCGGTATCTTCGGCCCGCCGCGCCAGCGCAGCGGCAATT
CAGGGCAGGCATTGACGGGCGCCGAGCGGCGCGTTTGGGTACTGCGCGACGGCCGGCCCGCACCTGTCGTCATCCAGGTC
GGCTCGTCCGACGGCCAGTTCACCCAGGTCGTCTCCGGGGAGCTCAAGGAAAACGACGCCTTGGTGACCGACGCCACGAC
GCGTGCGAACTAG

Upstream 100 bases:

>100_bases
AATTCCGTGCCACTTCGGCGGCAACGCTAATGCCCGGTTCATCTGCGCTGTGCTTATTTCGCTGCAAGGGCCGACACGGC
CAATGACTATCGAGGATGAC

Downstream 100 bases:

>100_bases
GCGGAGAGACGGATGGCAAGCCCGCCGCTCATCGAATTCAGGCAGGTCTCGAAAGTCTACGGTGAGGGCGAGGCGGCAAT
CCGCGCCCTCGACCATGTCG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNDTGTGKKTGPQTSGASDLAAVLAASGRQGKRSRWRGRLFILLILVAAAAGAAYFYMGRGQSEVSYATQPAKRGDLTVL
VTATGSVQPTEQVDISSELSGTVRDVNVDYNSTIKAGDVLALLDTNKLEADVKSSRAKLNSAKANVAKANADLESASTSL
ERLKSLVKSNVSTQQSLDDATYKYDSAVAAKQINEAEVLASEADLQLAEVNLAKAKIVSPIDGVILTRAVNPGATVAASL
SAPILFTIAGDLKKMELQVDVDEADVGQIAVGQKAKFTVDAYPDRSFPAEIEQIRFASEVVNNVVTYKAVLSVDNADLLL
RPGMTATADVTVEAVKDTLMVPNAALRYAPAQAGRRGRGIFGIFGPPRQRSGNSGQALTGAERRVWVLRDGRPAPVVIQV
GSSDGQFTQVVSGELKENDALVTDATTRAN

Sequences:

>Translated_430_residues
MNDTGTGKKTGPQTSGASDLAAVLAASGRQGKRSRWRGRLFILLILVAAAAGAAYFYMGRGQSEVSYATQPAKRGDLTVL
VTATGSVQPTEQVDISSELSGTVRDVNVDYNSTIKAGDVLALLDTNKLEADVKSSRAKLNSAKANVAKANADLESASTSL
ERLKSLVKSNVSTQQSLDDATYKYDSAVAAKQINEAEVLASEADLQLAEVNLAKAKIVSPIDGVILTRAVNPGATVAASL
SAPILFTIAGDLKKMELQVDVDEADVGQIAVGQKAKFTVDAYPDRSFPAEIEQIRFASEVVNNVVTYKAVLSVDNADLLL
RPGMTATADVTVEAVKDTLMVPNAALRYAPAQAGRRGRGIFGIFGPPRQRSGNSGQALTGAERRVWVLRDGRPAPVVIQV
GSSDGQFTQVVSGELKENDALVTDATTRAN
>Mature_430_residues
MNDTGTGKKTGPQTSGASDLAAVLAASGRQGKRSRWRGRLFILLILVAAAAGAAYFYMGRGQSEVSYATQPAKRGDLTVL
VTATGSVQPTEQVDISSELSGTVRDVNVDYNSTIKAGDVLALLDTNKLEADVKSSRAKLNSAKANVAKANADLESASTSL
ERLKSLVKSNVSTQQSLDDATYKYDSAVAAKQINEAEVLASEADLQLAEVNLAKAKIVSPIDGVILTRAVNPGATVAASL
SAPILFTIAGDLKKMELQVDVDEADVGQIAVGQKAKFTVDAYPDRSFPAEIEQIRFASEVVNNVVTYKAVLSVDNADLLL
RPGMTATADVTVEAVKDTLMVPNAALRYAPAQAGRRGRGIFGIFGPPRQRSGNSGQALTGAERRVWVLRDGRPAPVVIQV
GSSDGQFTQVVSGELKENDALVTDATTRAN

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=317, Percent_Identity=30.9148264984227, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789929, Length=284, Percent_Identity=27.112676056338, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082036, Length=286, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789041, Length=288, Percent_Identity=25, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45253; Mature: 45253

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTGTGKKTGPQTSGASDLAAVLAASGRQGKRSRWRGRLFILLILVAAAAGAAYFYMGR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
GQSEVSYATQPAKRGDLTVLVTATGSVQPTEQVDISSELSGTVRDVNVDYNSTIKAGDVL
CCCCCHHCCCCCCCCCEEEEEEECCCCCCCHHCCCHHHCCCCEEEEECCCCCCCCCCCEE
ALLDTNKLEADVKSSRAKLNSAKANVAKANADLESASTSLERLKSLVKSNVSTQQSLDDA
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCHH
TYKYDSAVAAKQINEAEVLASEADLQLAEVNLAKAKIVSPIDGVILTRAVNPGATVAASL
HHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHCCCCCCEEEEEECCCCCEEEEEC
SAPILFTIAGDLKKMELQVDVDEADVGQIAVGQKAKFTVDAYPDRSFPAEIEQIRFASEV
CCCEEEEEECCCEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
VNNVVTYKAVLSVDNADLLLRPGMTATADVTVEAVKDTLMVPNAALRYAPAQAGRRGRGI
HHHHHEEEEEEEECCCCEEEECCCCCCCCEEHHHHHHHHCCCCHHHCCCCHHCCCCCCCE
FGIFGPPRQRSGNSGQALTGAERRVWVLRDGRPAPVVIQVGSSDGQFTQVVSGELKENDA
EEECCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCCCCEEEECCCCCCCCCE
LVTDATTRAN
EEECCCCCCC
>Mature Secondary Structure
MNDTGTGKKTGPQTSGASDLAAVLAASGRQGKRSRWRGRLFILLILVAAAAGAAYFYMGR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
GQSEVSYATQPAKRGDLTVLVTATGSVQPTEQVDISSELSGTVRDVNVDYNSTIKAGDVL
CCCCCHHCCCCCCCCCEEEEEEECCCCCCCHHCCCHHHCCCCEEEEECCCCCCCCCCCEE
ALLDTNKLEADVKSSRAKLNSAKANVAKANADLESASTSLERLKSLVKSNVSTQQSLDDA
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCHH
TYKYDSAVAAKQINEAEVLASEADLQLAEVNLAKAKIVSPIDGVILTRAVNPGATVAASL
HHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHCCCCCCEEEEEECCCCCEEEEEC
SAPILFTIAGDLKKMELQVDVDEADVGQIAVGQKAKFTVDAYPDRSFPAEIEQIRFASEV
CCCEEEEEECCCEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
VNNVVTYKAVLSVDNADLLLRPGMTATADVTVEAVKDTLMVPNAALRYAPAQAGRRGRGI
HHHHHEEEEEEEECCCCEEEECCCCCCCCEEHHHHHHHHCCCCHHHCCCCHHCCCCCCCE
FGIFGPPRQRSGNSGQALTGAERRVWVLRDGRPAPVVIQVGSSDGQFTQVVSGELKENDA
EEECCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCCCCEEEECCCCCCCCCE
LVTDATTRAN
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]