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The map label for this gene is cysG [H]

Identifier: 209549235

GI number: 209549235

Start: 1666399

End: 1667835

Strand: Direct

Name: cysG [H]

Synonym: Rleg2_1636

Alternate gene names: 209549235

Gene position: 1666399-1667835 (Clockwise)

Preceding gene: 209549232

Following gene: 209549236

Centisome position: 36.72

GC content: 66.04

Gene sequence:

>1437_bases
ATGTCTCCCAAGACTGAGCAGCTTTCGGTATTTCCAGCCTTCTTTCGCGTGGAAGGCCAGAAGACGGCTGTCTTCGGCAA
TGGTGACGAGGCTTTCGCCAAGGTGCGGCTGCTGCTCAACACGCGGGCGCGGATCGTCGCCTATGCCGACAGGCCGGAAG
CCGATTATCATGCTTTCCTGATTGCCAACCGCATCGAGACTGTGCGCGCCGGCTTTTCCGCCGAGCAGGTCGCGGGTTCG
GCACTGGTCTTTGCCGCCACCGGCGATGAGGCCGATGATCGCGAGATCGTCGATGCCGCCCGTGCTGCTAGAATTCCGGC
CAATGCCGTCGATCAGCCGGATTACTGCGATTTCTTTACCCCGGCGCTGGTCAATCGCGCGCCCGTCGCCGTGGCGATCG
GCACCGAAGGGGCAGGGCCGGTTCTGGCGCAGATGATCCGGGCGCAGATCGATCAACTGCTTTCCCCCTCGCTCGGGCGG
CTTGCCGGCCTGGCGACGAGCTATCGCAAATCAGTCGAGCAGCTGGTGCCCCGTGGCGTTTCCCGCCGCGTCTTCTGGCG
CCGCTTCTTCTCCGGCGCCGTTGCCGATGCGGTCGCCAACGGCAACCTGCCGCAGGCCCGCCACGCCGCCGACCGGCTGC
TGGGCTCGATGGACAAGGTCGCCGGCCATATCTGGCTGGTCGGCGCCGGCCCAGGCGCCGAGGATCTGCTGACGCTGCGC
GCCCAGCGCGTGATGATGGAAGCCGATGTCATCGTCTATGACGCGCTCGTGCCGCAGGCGATCGTCGATATGGGTCGCCG
CGATGCCGAGCGGCTTTCGGTCGGCAAGCGCAAGGGCTGCCATTCGAAATCGCAGGAAGAGATCAACGACCTCCTGGTCG
ATCTCGGCCGCCAGGGCAAACGCGTCGTCCGCCTGAAATCAGGCGATCCGCTGGTCTATGGCCGGGCAGGGGAAGAGATG
GCGGCGTTGCGCGCTGCCGGCATCACCTATGAGGTCGTGCCCGGCATCACCTCGGCCTTCGCCGCCGCCGCCGATTTCGA
GCTGCCGCTGACGCTGCGCGGCGTCGCTTCGTCACTTGTTTTCACCACCGGCCATGATCTCACCGGCGACGTACTGCCCG
ATTGGGCAAGCCTTGCCGTCTCCGGCGCGACGATCGCCGTCTATATGGGCCGCACGGTTGCCGCCTCGGTCGCCGAGCGG
CTGATGCAGGCCGGCATTCCCTCCGAGACCACGGTCGCCGTCATCGAAAATGCCAGCCGCGCGGAGCGCCGCCTGCTGCA
TGGCACGCTTGCCGATCTGCCCGATCTGCAGCATCGCGACGAGTTGACCGGCCCGGTTATGGTCATTATCGGCGATGCGG
TCGCCGGCGCCAATTTCGAACTGTCCGAGCCGCTGGTGCGTGCGAACGCCCGGCTCGAGGAACTTGCAAGGAGCTGA

Upstream 100 bases:

>100_bases
CGAGCAAAAGAATACGCGCTTGCTGGGGTTTTCGAATTTCTGTTCCTTCAATCCCTTGTCGATCACGGGCATATTCTGGC
AACTTCGAAGTGGATTGATG

Downstream 100 bases:

>100_bases
ATATGGTAGACAAGGTTCTGACGGCCAACCGGCTGACGGATGGCATTGCCGTCTGGCTGGATGCGAACGGGAAGTGGTCC
ACCTCGCTGCAGGAAGCGCT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSPKTEQLSVFPAFFRVEGQKTAVFGNGDEAFAKVRLLLNTRARIVAYADRPEADYHAFLIANRIETVRAGFSAEQVAGS
ALVFAATGDEADDREIVDAARAARIPANAVDQPDYCDFFTPALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGR
LAGLATSYRKSVEQLVPRGVSRRVFWRRFFSGAVADAVANGNLPQARHAADRLLGSMDKVAGHIWLVGAGPGAEDLLTLR
AQRVMMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQEEINDLLVDLGRQGKRVVRLKSGDPLVYGRAGEEM
AALRAAGITYEVVPGITSAFAAAADFELPLTLRGVASSLVFTTGHDLTGDVLPDWASLAVSGATIAVYMGRTVAASVAER
LMQAGIPSETTVAVIENASRAERRLLHGTLADLPDLQHRDELTGPVMVIIGDAVAGANFELSEPLVRANARLEELARS

Sequences:

>Translated_478_residues
MSPKTEQLSVFPAFFRVEGQKTAVFGNGDEAFAKVRLLLNTRARIVAYADRPEADYHAFLIANRIETVRAGFSAEQVAGS
ALVFAATGDEADDREIVDAARAARIPANAVDQPDYCDFFTPALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGR
LAGLATSYRKSVEQLVPRGVSRRVFWRRFFSGAVADAVANGNLPQARHAADRLLGSMDKVAGHIWLVGAGPGAEDLLTLR
AQRVMMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQEEINDLLVDLGRQGKRVVRLKSGDPLVYGRAGEEM
AALRAAGITYEVVPGITSAFAAAADFELPLTLRGVASSLVFTTGHDLTGDVLPDWASLAVSGATIAVYMGRTVAASVAER
LMQAGIPSETTVAVIENASRAERRLLHGTLADLPDLQHRDELTGPVMVIIGDAVAGANFELSEPLVRANARLEELARS
>Mature_477_residues
SPKTEQLSVFPAFFRVEGQKTAVFGNGDEAFAKVRLLLNTRARIVAYADRPEADYHAFLIANRIETVRAGFSAEQVAGSA
LVFAATGDEADDREIVDAARAARIPANAVDQPDYCDFFTPALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGRL
AGLATSYRKSVEQLVPRGVSRRVFWRRFFSGAVADAVANGNLPQARHAADRLLGSMDKVAGHIWLVGAGPGAEDLLTLRA
QRVMMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQEEINDLLVDLGRQGKRVVRLKSGDPLVYGRAGEEMA
ALRAAGITYEVVPGITSAFAAAADFELPLTLRGVASSLVFTTGHDLTGDVLPDWASLAVSGATIAVYMGRTVAASVAERL
MQAGIPSETTVAVIENASRAERRLLHGTLADLPDLQHRDELTGPVMVIIGDAVAGANFELSEPLVRANARLEELARS

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=445, Percent_Identity=38.2022471910112, Blast_Score=294, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=30.672268907563, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51044; Mature: 50913

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKTEQLSVFPAFFRVEGQKTAVFGNGDEAFAKVRLLLNTRARIVAYADRPEADYHAFL
CCCCCCHHEEEEHEEEECCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
IANRIETVRAGFSAEQVAGSALVFAATGDEADDREIVDAARAARIPANAVDQPDYCDFFT
HHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
PALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGRLAGLATSYRKSVEQLVPRGV
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
SRRVFWRRFFSGAVADAVANGNLPQARHAADRLLGSMDKVAGHIWLVGAGPGAEDLLTLR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
AQRVMMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQEEINDLLVDLGRQGK
HHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCC
RVVRLKSGDPLVYGRAGEEMAALRAAGITYEVVPGITSAFAAAADFELPLTLRGVASSLV
EEEEEECCCCEEECCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEHHHHHHHHE
FTTGHDLTGDVLPDWASLAVSGATIAVYMGRTVAASVAERLMQAGIPSETTVAVIENASR
EECCCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
AERRLLHGTLADLPDLQHRDELTGPVMVIIGDAVAGANFELSEPLVRANARLEELARS
HHHHHHHHHHHCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPKTEQLSVFPAFFRVEGQKTAVFGNGDEAFAKVRLLLNTRARIVAYADRPEADYHAFL
CCCCCHHEEEEHEEEECCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
IANRIETVRAGFSAEQVAGSALVFAATGDEADDREIVDAARAARIPANAVDQPDYCDFFT
HHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
PALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGRLAGLATSYRKSVEQLVPRGV
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
SRRVFWRRFFSGAVADAVANGNLPQARHAADRLLGSMDKVAGHIWLVGAGPGAEDLLTLR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
AQRVMMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQEEINDLLVDLGRQGK
HHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCC
RVVRLKSGDPLVYGRAGEEMAALRAAGITYEVVPGITSAFAAAADFELPLTLRGVASSLV
EEEEEECCCCEEECCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEHHHHHHHHE
FTTGHDLTGDVLPDWASLAVSGATIAVYMGRTVAASVAERLMQAGIPSETTVAVIENASR
EECCCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
AERRLLHGTLADLPDLQHRDELTGPVMVIIGDAVAGANFELSEPLVRANARLEELARS
HHHHHHHHHHHCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA