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The map label for this gene is lpdA [C]

Identifier: 209549207

GI number: 209549207

Start: 1635181

End: 1636626

Strand: Direct

Name: lpdA [C]

Synonym: Rleg2_1608

Alternate gene names: 209549207

Gene position: 1635181-1636626 (Clockwise)

Preceding gene: 209549206

Following gene: 209549208

Centisome position: 36.03

GC content: 62.59

Gene sequence:

>1446_bases
ATGGCTGAATCCTACGACGTCATCATCATCGGCTCCGGTCCCGGCGGCTATGTCGCCGCCATCCGCGCCGCTCAGCTCGG
CCTCAAGACCGCGATCGTCGAGCGCGAGCATATGGGCGGTATCTGCCTGAACTGGGGCTGCATCCCCACCAAGGCGCTGC
TGCGCTCGGCCGAGGTGCTCGATCACGCCAATCACTTCAAGGATTTCGGCCTCGTTCTCGAAGGTACGGTCAAGCCGGAC
GCCAAGGCCGTCGTTGGCCGCTCGCGCGCCGTCTCCGCCCGTTTGAATGCCGGCGTCGGCTTCCTGATGAAGAAGAACAA
GATCGACATCATCTGGGGCGAGGCAAAGATCACTAAACCTGGCGAGATCGTCGTCGGCAAGTCATCGAAGCCCGTCGTCG
AGCCGCAGCACCCGCTGCCGAAGAACGTCAAAGGCGAGGGCACCTACACCGCCAAGCACATCATCATCGCCACCGGCGCC
CGCCCGCGTGCGCTGCCCGGCATCGAGCCCGACGGCAAGTTGATCTGGACCTATTTCGAGGCGCTGAAGCCGGATGCGCT
GCCGAAGTCGCTGATCGTCATGGGCTCGGGCGCGATCGGCATCGAATTCGCCAGCTTCTATCGCTCGATGGGCGTTGATG
TGACGGTTGTCGAAGTCATGCCGACCATCATGCCGGTCGAAGATGCCGAAATCACCGCCATCGCCCGCAAGCAGCTTGAA
AAGCGCGGCCTCAAGATCTTCACCAGCGCCAAGGTGACGAAGGTCGAGAAGGGCGCGGGCAGCATCACCGCCCATGTCGA
GACTTCAGACGGCAAGGTGCAGCAGATCACCGCCGACCGGATGATTTCGGCCGTCGGCGTTCAGGGTAATATCGAAAATC
TCGGCCTCGAGGCGCTTGGCATCAAGACCGATCGCGGCTGCGTCGTCGCCGACGGTTACGGCAAGACCAATGTCGCCGGC
ATATATGCGATCGGCGACGTTGCCGGCCCGCCGATGCTGGCGCACAAGGCCGAGCATGAAGGCGTCGTTTGCATCGAGAA
GATCGCCGGTCTGCCGCATGTTCATCCGACCGACAAGGGCAAGGTTCCCGGCTGCACCTATTGCAATCCGCAGGTGGCCT
CCGTCGGCCTCACCGAAGCCAAGGCCAAGGAACTGGGCCGCGACATCCGCGTCGGCCGCTTCTCCTTCGCGGCAAACGGC
AAGGCGATCGCGCTCGGCGAAGACCAGGGCATGGTGAAGGTGATCTTCGACAAGAAGACCGGCGAACTGCTCGGCGCCCA
CATGGTCGGCGCCGAAGTCACCGAGCTCATCCAGGGCTTCGTCGTCGCGATGAACCTGGAAACAACAGAGGAAGAACTGA
TGCACACCATCTTCCCGCACCCGACCGTTTCCGAAACGATGAAGGAAGCGGTGCTCGATGCCTATGGCCGCGTGCTGAAC
GCTTGA

Upstream 100 bases:

>100_bases
GAGGCTGCGCGCGCCGATGACGTGCCGCTCTTCGAAAACAGCAAGAGCCACGCGTGGGTGCTCATGACCCGCAGCGCAGC
ATAACAAGGGCAGGAAACAC

Downstream 100 bases:

>100_bases
TAATTTCCACGGCTGCCCTCTATCCCAGGGCGGCAAGCCGTCCCGCAGAGGCGGCGAAGCCATCCTCCGGGATGGCCTAG
GCCATCCCCGGGGGCTGGAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; ORF-E3 [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAESYDVIIIGSGPGGYVAAIRAAQLGLKTAIVEREHMGGICLNWGCIPTKALLRSAEVLDHANHFKDFGLVLEGTVKPD
AKAVVGRSRAVSARLNAGVGFLMKKNKIDIIWGEAKITKPGEIVVGKSSKPVVEPQHPLPKNVKGEGTYTAKHIIIATGA
RPRALPGIEPDGKLIWTYFEALKPDALPKSLIVMGSGAIGIEFASFYRSMGVDVTVVEVMPTIMPVEDAEITAIARKQLE
KRGLKIFTSAKVTKVEKGAGSITAHVETSDGKVQQITADRMISAVGVQGNIENLGLEALGIKTDRGCVVADGYGKTNVAG
IYAIGDVAGPPMLAHKAEHEGVVCIEKIAGLPHVHPTDKGKVPGCTYCNPQVASVGLTEAKAKELGRDIRVGRFSFAANG
KAIALGEDQGMVKVIFDKKTGELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTVSETMKEAVLDAYGRVLN
A

Sequences:

>Translated_481_residues
MAESYDVIIIGSGPGGYVAAIRAAQLGLKTAIVEREHMGGICLNWGCIPTKALLRSAEVLDHANHFKDFGLVLEGTVKPD
AKAVVGRSRAVSARLNAGVGFLMKKNKIDIIWGEAKITKPGEIVVGKSSKPVVEPQHPLPKNVKGEGTYTAKHIIIATGA
RPRALPGIEPDGKLIWTYFEALKPDALPKSLIVMGSGAIGIEFASFYRSMGVDVTVVEVMPTIMPVEDAEITAIARKQLE
KRGLKIFTSAKVTKVEKGAGSITAHVETSDGKVQQITADRMISAVGVQGNIENLGLEALGIKTDRGCVVADGYGKTNVAG
IYAIGDVAGPPMLAHKAEHEGVVCIEKIAGLPHVHPTDKGKVPGCTYCNPQVASVGLTEAKAKELGRDIRVGRFSFAANG
KAIALGEDQGMVKVIFDKKTGELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTVSETMKEAVLDAYGRVLN
A
>Mature_480_residues
AESYDVIIIGSGPGGYVAAIRAAQLGLKTAIVEREHMGGICLNWGCIPTKALLRSAEVLDHANHFKDFGLVLEGTVKPDA
KAVVGRSRAVSARLNAGVGFLMKKNKIDIIWGEAKITKPGEIVVGKSSKPVVEPQHPLPKNVKGEGTYTAKHIIIATGAR
PRALPGIEPDGKLIWTYFEALKPDALPKSLIVMGSGAIGIEFASFYRSMGVDVTVVEVMPTIMPVEDAEITAIARKQLEK
RGLKIFTSAKVTKVEKGAGSITAHVETSDGKVQQITADRMISAVGVQGNIENLGLEALGIKTDRGCVVADGYGKTNVAGI
YAIGDVAGPPMLAHKAEHEGVVCIEKIAGLPHVHPTDKGKVPGCTYCNPQVASVGLTEAKAKELGRDIRVGRFSFAANGK
AIALGEDQGMVKVIFDKKTGELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTVSETMKEAVLDAYGRVLNA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=484, Percent_Identity=39.0495867768595, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI50301238, Length=482, Percent_Identity=29.4605809128631, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI291045266, Length=443, Percent_Identity=26.1851015801354, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI33519430, Length=467, Percent_Identity=25.9100642398287, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI33519428, Length=467, Percent_Identity=25.9100642398287, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI33519426, Length=467, Percent_Identity=25.9100642398287, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI148277065, Length=467, Percent_Identity=25.9100642398287, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=25.9100642398287, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI22035672, Length=471, Percent_Identity=25.6900212314225, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI291045268, Length=487, Percent_Identity=26.0780287474333, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=35.9913793103448, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082354, Length=485, Percent_Identity=26.5979381443299, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789915, Length=477, Percent_Identity=30.188679245283, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87081717, Length=470, Percent_Identity=28.0851063829787, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=485, Percent_Identity=37.9381443298969, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17557007, Length=489, Percent_Identity=26.7893660531697, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=463, Percent_Identity=25.4859611231102, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=463, Percent_Identity=25.4859611231102, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=502, Percent_Identity=25.4980079681275, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=488, Percent_Identity=36.2704918032787, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6325166, Length=484, Percent_Identity=28.099173553719, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325240, Length=486, Percent_Identity=27.3662551440329, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358499, Length=482, Percent_Identity=39.8340248962656, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24640553, Length=484, Percent_Identity=27.0661157024793, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24640549, Length=484, Percent_Identity=27.0661157024793, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=27.1221532091097, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI17737741, Length=490, Percent_Identity=25.9183673469388, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51006; Mature: 50875

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESYDVIIIGSGPGGYVAAIRAAQLGLKTAIVEREHMGGICLNWGCIPTKALLRSAEVL
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
DHANHFKDFGLVLEGTVKPDAKAVVGRSRAVSARLNAGVGFLMKKNKIDIIWGEAKITKP
HHHHHHHHCCEEEEECCCCCCHHHHCCCCEEEEECCCCEEEEEECCCEEEEECCEEECCC
GEIVVGKSSKPVVEPQHPLPKNVKGEGTYTAKHIIIATGARPRALPGIEPDGKLIWTYFE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEHH
ALKPDALPKSLIVMGSGAIGIEFASFYRSMGVDVTVVEVMPTIMPVEDAEITAIARKQLE
HHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
KRGLKIFTSAKVTKVEKGAGSITAHVETSDGKVQQITADRMISAVGVQGNIENLGLEALG
HCCCEEEECCEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCCCHHHCCEEEEE
IKTDRGCVVADGYGKTNVAGIYAIGDVAGPPMLAHKAEHEGVVCIEKIAGLPHVHPTDKG
EECCCCEEEECCCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCC
KVPGCTYCNPQVASVGLTEAKAKELGRDIRVGRFSFAANGKAIALGEDQGMVKVIFDKKT
CCCCCCCCCCCHHCCCCCHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCEEEEEEECCC
GELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTVSETMKEAVLDAYGRVLN
CHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure 
AESYDVIIIGSGPGGYVAAIRAAQLGLKTAIVEREHMGGICLNWGCIPTKALLRSAEVL
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
DHANHFKDFGLVLEGTVKPDAKAVVGRSRAVSARLNAGVGFLMKKNKIDIIWGEAKITKP
HHHHHHHHCCEEEEECCCCCCHHHHCCCCEEEEECCCCEEEEEECCCEEEEECCEEECCC
GEIVVGKSSKPVVEPQHPLPKNVKGEGTYTAKHIIIATGARPRALPGIEPDGKLIWTYFE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEHH
ALKPDALPKSLIVMGSGAIGIEFASFYRSMGVDVTVVEVMPTIMPVEDAEITAIARKQLE
HHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
KRGLKIFTSAKVTKVEKGAGSITAHVETSDGKVQQITADRMISAVGVQGNIENLGLEALG
HCCCEEEECCEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCCCHHHCCEEEEE
IKTDRGCVVADGYGKTNVAGIYAIGDVAGPPMLAHKAEHEGVVCIEKIAGLPHVHPTDKG
EECCCCEEEECCCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCC
KVPGCTYCNPQVASVGLTEAKAKELGRDIRVGRFSFAANGKAIALGEDQGMVKVIFDKKT
CCCCCCCCCCCHHCCCCCHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCEEEEEEECCC
GELLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTVSETMKEAVLDAYGRVLN
CHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9141657 [H]