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The map label for this gene is eno [H]

Identifier: 209549200

GI number: 209549200

Start: 1628241

End: 1629515

Strand: Direct

Name: eno [H]

Synonym: Rleg2_1601

Alternate gene names: 209549200

Gene position: 1628241-1629515 (Clockwise)

Preceding gene: 209549199

Following gene: 209549201

Centisome position: 35.88

GC content: 63.37

Gene sequence:

>1275_bases
ATGACTGCAATCACCGATATCATCGCCCGCGAGATTCTCGATAGCCGTGGCAACCCCACCGTCGAAGTCGATGTCTATCT
CGAAGACGGCAGCATGGGCCGCGCGGCCGTTCCCTCGGGCGCTTCGACGGGCGCGCATGAGGCGGTCGAGCTGCGCGACG
GCGGCAAGCGCTACCTCGGCAAGGGCGTGCAGAAGGCGGTCGATGCGGCCAATACCGAGATCTTCGACGCGATCGGCGGC
ATCGATGCCGAAAACCAGATCCAGATCGACAACATCATGATCGAGCTCGACGGCACGCCGAACAAGTCGCGCCTCGGCGC
CAACGCCATTCTCGGCGTGTCGCTGGCCGTCGCCAAGGCTGCCGCCCAGGCCTCCGGCCTGCCGCTCTACCGTTACGTCG
GCGGCGCGTCCGCGTGCCTGCTTCCGGTGCCGATGATGAACATCATCAACGGCGGCGCCCATGCCGACAATCCAATCGAT
TTCCAGGAATTCATGATCCTGCCGGTCGGCGCCGACACGATCGCTGAAGCCGTGCGCATGGGTTCGGAAGTCTTCCATAC
CTTGCGCAAGGAACTCGCCGCCCAGGGTCACAACACCAATGTCGGCGACGAAGGTGGTTTTGCTCCGGGTCTGAAGAGCG
CGCCTGAAGCCCTCGACTTCATCATGAAGTCGATCGAAAAGGCCGGCTACAAGCCGGGCGACGACATGTGCCTCGGCCTC
GATTGCGCCTCGACTGAGTTCTTCAAGGACGGCAAATATGTTCTCGAAGGCGAAGGCCGCACGCTCGAATCGGGCGCCAT
GGCCGAATATCTGGCCGAGCTCGCGGCCAAGTACCCGATCATCTCGATCGAGGATGGCATGGCCGAGGACGACTGGGATG
GCTGGAAGACGCTGACCGACCTGACCGGCAAGAAGACCCAGCTCGTCGGCGACGATCTCTTCGTCACCAACTCCGCCCGT
CTTCGCGACGGCATCCGCATGGGCGTCGCCAACTCGATCCTCGTCAAGGTCAACCAGATCGGCTCGCTGACGGAAACGCT
CGACGCCGTCAACACGGCGCACAAGGCAGCCTATACCGCCGTCATGTCGCACCGCTCCGGCGAAACCGAAGATTCGACCA
TCGCCGACCTCGCGGTCGCTACCAACTGCGGCCAGATCAAGACCGGCTCGCTGTCGCGTTCCGACCGTCTCGCCAAGTAC
AACCAGCTGATCCGCATCGAGGAAGGCCTCGGCCCTCAGGCTCAGTATGCCGGCCGCTCGATCATCCGCGGCTGA

Upstream 100 bases:

>100_bases
GACATGCAGATCGAAGGTGTGCAATGCGCCATTGTAATTTGCACGCCTTCGATTATGACAGGCTTCGAACGATTGATCAC
CCACCGAGCAGGAAGAACCC

Downstream 100 bases:

>100_bases
TCGAATCTGCTTTCGACCACGTAACCCGCGCCTCCCGGCGCGGGTTTTTTGTTGCCCGTATTTACGGTCAACGAACGGTT
AATCTCTGAGCGTTATGCTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVQKAVDAANTEIFDAIGG
IDAENQIQIDNIMIELDGTPNKSRLGANAILGVSLAVAKAAAQASGLPLYRYVGGASACLLPVPMMNIINGGAHADNPID
FQEFMILPVGADTIAEAVRMGSEVFHTLRKELAAQGHNTNVGDEGGFAPGLKSAPEALDFIMKSIEKAGYKPGDDMCLGL
DCASTEFFKDGKYVLEGEGRTLESGAMAEYLAELAAKYPIISIEDGMAEDDWDGWKTLTDLTGKKTQLVGDDLFVTNSAR
LRDGIRMGVANSILVKVNQIGSLTETLDAVNTAHKAAYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRLAKY
NQLIRIEEGLGPQAQYAGRSIIRG

Sequences:

>Translated_424_residues
MTAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVQKAVDAANTEIFDAIGG
IDAENQIQIDNIMIELDGTPNKSRLGANAILGVSLAVAKAAAQASGLPLYRYVGGASACLLPVPMMNIINGGAHADNPID
FQEFMILPVGADTIAEAVRMGSEVFHTLRKELAAQGHNTNVGDEGGFAPGLKSAPEALDFIMKSIEKAGYKPGDDMCLGL
DCASTEFFKDGKYVLEGEGRTLESGAMAEYLAELAAKYPIISIEDGMAEDDWDGWKTLTDLTGKKTQLVGDDLFVTNSAR
LRDGIRMGVANSILVKVNQIGSLTETLDAVNTAHKAAYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRLAKY
NQLIRIEEGLGPQAQYAGRSIIRG
>Mature_423_residues
TAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVQKAVDAANTEIFDAIGGI
DAENQIQIDNIMIELDGTPNKSRLGANAILGVSLAVAKAAAQASGLPLYRYVGGASACLLPVPMMNIINGGAHADNPIDF
QEFMILPVGADTIAEAVRMGSEVFHTLRKELAAQGHNTNVGDEGGFAPGLKSAPEALDFIMKSIEKAGYKPGDDMCLGLD
CASTEFFKDGKYVLEGEGRTLESGAMAEYLAELAAKYPIISIEDGMAEDDWDGWKTLTDLTGKKTQLVGDDLFVTNSARL
RDGIRMGVANSILVKVNQIGSLTETLDAVNTAHKAAYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRLAKYN
QLIRIEEGLGPQAQYAGRSIIRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.3888888888889, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=50.6944444444444, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=50.6944444444444, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=50.6944444444444, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=46.5116279069767, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=24.9258160237389, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=24.9258160237389, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=24.9258160237389, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=63.2558139534884, Blast_Score=517, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.5462962962963, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=52.5462962962963, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.875, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=51.25284738041, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=50.7972665148064, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=50.7972665148064, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=48.74715261959, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=439, Percent_Identity=48.5193621867882, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=50.2304147465438, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=50.2304147465438, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=50.2304147465438, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=50.2304147465438, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=50.2304147465438, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=50.2304147465438, Blast_Score=376, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 44831; Mature: 44700

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVQKAVDAANTEIFDAIGGIDAENQIQIDNIMIELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AAQASGLPLYRYVGGASACLLPVPMMNIINGGAHADNPIDFQEFMILPVGADTIAEAVRM
HHHHCCCCCEEHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
GSEVFHTLRKELAAQGHNTNVGDEGGFAPGLKSAPEALDFIMKSIEKAGYKPGDDMCLGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEC
DCASTEFFKDGKYVLEGEGRTLESGAMAEYLAELAAKYPIISIEDGMAEDDWDGWKTLTD
CCCCCHHHCCCCEEEECCCCEECCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHH
LTGKKTQLVGDDLFVTNSARLRDGIRMGVANSILVKVNQIGSLTETLDAVNTAHKAAYTA
CCCCCEEECCCCEEEECCHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRLAKYNQLIRIEEGLGPQAQYAGRS
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHCCHH
IIRG
HCCC
>Mature Secondary Structure 
TAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVQKAVDAANTEIFDAIGGIDAENQIQIDNIMIELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AAQASGLPLYRYVGGASACLLPVPMMNIINGGAHADNPIDFQEFMILPVGADTIAEAVRM
HHHHCCCCCEEHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
GSEVFHTLRKELAAQGHNTNVGDEGGFAPGLKSAPEALDFIMKSIEKAGYKPGDDMCLGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEC
DCASTEFFKDGKYVLEGEGRTLESGAMAEYLAELAAKYPIISIEDGMAEDDWDGWKTLTD
CCCCCHHHCCCCEEEECCCCEECCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHH
LTGKKTQLVGDDLFVTNSARLRDGIRMGVANSILVKVNQIGSLTETLDAVNTAHKAAYTA
CCCCCEEECCCCEEEECCHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRLAKYNQLIRIEEGLGPQAQYAGRS
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHCCHH
IIRG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]