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The map label for this gene is tsf [H]

Identifier: 209549183

GI number: 209549183

Start: 1609290

End: 1610216

Strand: Direct

Name: tsf [H]

Synonym: Rleg2_1584

Alternate gene names: 209549183

Gene position: 1609290-1610216 (Clockwise)

Preceding gene: 209549182

Following gene: 209549184

Centisome position: 35.46

GC content: 63.0

Gene sequence:

>927_bases
ATGAGCGAGATTACGGCTGCAATGGTGAAGGAACTGCGCGAAAAGACCGGCGCAGGCATGATGGACTGCAAGAAGGCTCT
TGCTGAAACCGGCGGCGACATGGAAGCGGCGATCGACTGGCTGCGCGCCAAGGGCATCGCCAAGGCCGACAAGAAGTCGG
GCCGCACCGCTGCCGAAGGCCTCATCGGCGTTTCGAGCCAGGGCACCAAGGCTGTTGTCGTCGAAGTCAATTCCGAAACC
GACTTCGTCGCCCGTAACGATGCCTTCCAGGATCTCGTCCGCGGCATTGCCAAGGTTGCCGTCTCCACCAACGGCAGCGT
CGACGCCGTTGCGGCTGCGACCTACCCGGCCTCCGGCAAGTCGGTTTCCGACACGATCAAGGACGCGATCGCAACGATCG
GCGAGAACATGAACCTGCGCCGCTCGGTCGCGCTCTCCGTCGAGGACGGCGTCGTCGCCACCTATATCCACAATGCTGTT
TCCGACGGCCTCGGAAAGCTCGGCGTTCTCGTCGCGCTGAAGTCGACCGGCGACAAGGAAGCCCTGAACGCAATCGGCCG
CCAGGTCGCCATGCACATCGCTGCGACCGCACCGCTGGCGATCCGCCCCGAGGAAGTCGATGCCGCCGTCGCCGAGCGCG
AGCGCAACGTCTTCATCGAGCAGTCGCGCGCTTCCGGGAAGCCTGATAACATCATCGAGAAGATGGTCGATGGCCGCATG
CGCAAGTTCTTCGAAGAAGTCGCCCTTCTCTCGCAGGCTTTCGTCATCAATCCGGATCTGACCGTCGCAGCCGCCGTCAA
GGAAGCTGAAAAGGCCGTCGGCGCGCCGATCGAAGTCGCCGGCATGGCCCGCCTTCTGCTCGGCGAAGGTGTCGAGAAGG
AAGAAACCGATTTCGCAGCCGAAGTTGCGGCTGCCGTCAAGGGTTGA

Upstream 100 bases:

>100_bases
GTCTTCATCACGCTGTCATACATACAGGTACATTTCGTGCCTCAATCCGGTGCCGCGCCGCGCTTTGCGGCCCACCGTAT
GAACCGACAAGAGGAAGCTA

Downstream 100 bases:

>100_bases
TCTTCCAGCCATAGTTGGGAAAACAACAGGGGCATCGCGTGACAACGCGGTGCCCTTCGTGTATCGGGCACTCACGGCAA
TTTACGAGGAGCCAAGATGT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSEITAAMVKELREKTGAGMMDCKKALAETGGDMEAAIDWLRAKGIAKADKKSGRTAAEGLIGVSSQGTKAVVVEVNSET
DFVARNDAFQDLVRGIAKVAVSTNGSVDAVAAATYPASGKSVSDTIKDAIATIGENMNLRRSVALSVEDGVVATYIHNAV
SDGLGKLGVLVALKSTGDKEALNAIGRQVAMHIAATAPLAIRPEEVDAAVAERERNVFIEQSRASGKPDNIIEKMVDGRM
RKFFEEVALLSQAFVINPDLTVAAAVKEAEKAVGAPIEVAGMARLLLGEGVEKEETDFAAEVAAAVKG

Sequences:

>Translated_308_residues
MSEITAAMVKELREKTGAGMMDCKKALAETGGDMEAAIDWLRAKGIAKADKKSGRTAAEGLIGVSSQGTKAVVVEVNSET
DFVARNDAFQDLVRGIAKVAVSTNGSVDAVAAATYPASGKSVSDTIKDAIATIGENMNLRRSVALSVEDGVVATYIHNAV
SDGLGKLGVLVALKSTGDKEALNAIGRQVAMHIAATAPLAIRPEEVDAAVAERERNVFIEQSRASGKPDNIIEKMVDGRM
RKFFEEVALLSQAFVINPDLTVAAAVKEAEKAVGAPIEVAGMARLLLGEGVEKEETDFAAEVAAAVKG
>Mature_307_residues
SEITAAMVKELREKTGAGMMDCKKALAETGGDMEAAIDWLRAKGIAKADKKSGRTAAEGLIGVSSQGTKAVVVEVNSETD
FVARNDAFQDLVRGIAKVAVSTNGSVDAVAAATYPASGKSVSDTIKDAIATIGENMNLRRSVALSVEDGVVATYIHNAVS
DGLGKLGVLVALKSTGDKEALNAIGRQVAMHIAATAPLAIRPEEVDAAVAERERNVFIEQSRASGKPDNIIEKMVDGRMR
KFFEEVALLSQAFVINPDLTVAAAVKEAEKAVGAPIEVAGMARLLLGEGVEKEETDFAAEVAAAVKG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=220, Percent_Identity=35.4545454545455, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI291084500, Length=241, Percent_Identity=33.6099585062241, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI291084502, Length=94, Percent_Identity=47.8723404255319, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI291084498, Length=94, Percent_Identity=47.8723404255319, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1786366, Length=307, Percent_Identity=44.9511400651466, Blast_Score=224, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17561440, Length=278, Percent_Identity=30.2158273381295, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19921466, Length=310, Percent_Identity=27.0967741935484, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32098; Mature: 31967

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEITAAMVKELREKTGAGMMDCKKALAETGGDMEAAIDWLRAKGIAKADKKSGRTAAEG
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHH
LIGVSSQGTKAVVVEVNSETDFVARNDAFQDLVRGIAKVAVSTNGSVDAVAAATYPASGK
CEECCCCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
SVSDTIKDAIATIGENMNLRRSVALSVEDGVVATYIHNAVSDGLGKLGVLVALKSTGDKE
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHH
ALNAIGRQVAMHIAATAPLAIRPEEVDAAVAERERNVFIEQSRASGKPDNIIEKMVDGRM
HHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHH
RKFFEEVALLSQAFVINPDLTVAAAVKEAEKAVGAPIEVAGMARLLLGEGVEKEETDFAA
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
EVAAAVKG
HHHHHHCC
>Mature Secondary Structure 
SEITAAMVKELREKTGAGMMDCKKALAETGGDMEAAIDWLRAKGIAKADKKSGRTAAEG
CHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHH
LIGVSSQGTKAVVVEVNSETDFVARNDAFQDLVRGIAKVAVSTNGSVDAVAAATYPASGK
CEECCCCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
SVSDTIKDAIATIGENMNLRRSVALSVEDGVVATYIHNAVSDGLGKLGVLVALKSTGDKE
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHH
ALNAIGRQVAMHIAATAPLAIRPEEVDAAVAERERNVFIEQSRASGKPDNIIEKMVDGRM
HHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHH
RKFFEEVALLSQAFVINPDLTVAAAVKEAEKAVGAPIEVAGMARLLLGEGVEKEETDFAA
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
EVAAAVKG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA