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The map label for this gene is 209549108

Identifier: 209549108

GI number: 209549108

Start: 1539179

End: 1540954

Strand: Reverse

Name: 209549108

Synonym: Rleg2_1507

Alternate gene names: NA

Gene position: 1540954-1539179 (Counterclockwise)

Preceding gene: 209549109

Following gene: 209549103

Centisome position: 33.96

GC content: 60.02

Gene sequence:

>1776_bases
TTGTCCCATGTCTTGGAAGCTGCGCGCCGACGTTTTCAGCTAATCCTGATCAAGCCGTCGCATTATGACGACGACGGCTA
TGTCATCCGCTGGTGGCGGGCGATGATCCCTTCCAATTCGCTCGCGGCTCTCTATGGCATCGCCGCCGAATGCGCTGAGC
GTAAGGTGCTCGGGCCCGATACGGCGATCGACATCACCGTGATCGACGAGACCAATACGCGGATCGACTTCGCCGGGCTG
CTCGCACAATTCAAGCGCCACGACAATTTCGGCATGATCGCGCTGGTCGGCGTCCAGACCAACCAGTATCCACGTGCGCT
CGATATCGCTCGGCCCTTCCGCGATGCCGGCCTGCCGGTTTCGATCGGCGGCTTCCATGTTTCCGGCTGCCTGTCGATGC
TGGACGGCAAGGCGGTCGGGCTCGAAGCCTGCCGTGATATGGGCATTTCGATGTTTGCCGGCGAGGCCGAGGGCCGGCTG
GAGATGGTGTTGCGCGATGCCGCGAGCGGCGAGCTGAAGCCGCTCTATAATTTCATGAACGACCTTCCCGGCATCGGCGG
CACGCCGGTTCCCTTCCTCCCGAAGGACAATATCCAGCGCACGCTTGGGCTCAGCACCAGCTTCGATGCCGGACGCGGCT
GTCCCTATCAGTGCTCGTTCTGCACCATCATCAACGTGCAGGGACGCAAGTCGCGTTTCCGCTCGGCCGATGACGTCGAA
AAGCTGGTGCGGATGAACTGGGCGCAGGGCATCCATAAATTCTTCATCACCGACGACAATTTCGCCCGCAACAAGGATTG
GGAAGCGATCTTCGACCGGCTGATCGAACTCAAGGAGCGGGACGGCATTCCGCTCGGCCTGATGATTCAGGTCGACACGC
TGTGCCACAAGATCCCGAATTTTATCGAGAAATCCCGGCGGGCCGGCGTCACCCGCGTTTTCATCGGCCTCGAAAACGTC
AACCCTGACAATCTGACGGCCGCCAAGAAGAACCAGAACAAGATCACCGAATATCGCAAGATGCTGCTCGCCTGGAAGGC
GCAGGGCATCATGACGCTCGCCGGTTATATCCTGGGCTTCCCCGCCGATACGCCGGAATCGATCCGCCGCGATATCGCGA
TCATCCAGGAAGAGCTGCCGCTCGACGTCATCGAATTCTTCATCCTGACGCCGCTGCCCGGCTCCGAGGATCATCAGGTT
CTATGGAAGAAGAATGTCGAGATGGACAGCGATCTCAACATCTACGATGTCGAGCACGTCTGCACCGCGCATCCGAAGAT
GAGCAAGCAGGAGTGGGAAGACATCTACCACGAGGCCTGGGCGCTCTATTATTCACCTGATCATATGAAGACGCTGCTGC
GCCGGGCCGTGGCGACCGGGGTACCGCTCGCAAGACTCGTCAAGGTTCTCGTCTCCTTCGCAACCACCGTGCCGCTGGAA
AACGTGCATCCGCTGCAGAGCGGCCTCCTGCGCTTGAAGACACCTTCAGAGCGACGCCCCGACCTGCCGCGCGAAAATCC
GCTGGTCTTCTGGCCGCGTTTTGCCTGGGACACCTTCAGAAAACATGCTTCGCTCGCCGGCACGATCATCGGCCTGACGA
TTTCGGCATTCCTGATTTCAAGGGAAGCGAAGTCGAAGACCTATATGGATCAGGCCCTGACGCCGGTCGCCGACGATGAG
GAAGAGACACTCAGCCTCTTCACCAAAACGGCCGGCGGCACTGCTGCCGTCAGCCATGTGAGGAAAGTCGCGGAGCTGAC
GCGGACCGCGCATTGA

Upstream 100 bases:

>100_bases
TTAGTTTGACCGCCTGAATCGTATTGATACGCCATGCCTGATAGATGCATTCAACGCCATTTTGCCTCCGGCAAAAGCTT
CTGAAGAAAGATAGATAATC

Downstream 100 bases:

>100_bases
ATCGCGATTCCGCTAAAGCGCGTCGCACGGATCACGTTCGACGCCATACGCTTTAGCGGCTTGAGGTTTGAGCGACCTGG
TTTATCTGGGCTATCTCAGC

Product: radical SAM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSHVLEAARRRFQLILIKPSHYDDDGYVIRWWRAMIPSNSLAALYGIAAECAERKVLGPDTAIDITVIDETNTRIDFAGL
LAQFKRHDNFGMIALVGVQTNQYPRALDIARPFRDAGLPVSIGGFHVSGCLSMLDGKAVGLEACRDMGISMFAGEAEGRL
EMVLRDAASGELKPLYNFMNDLPGIGGTPVPFLPKDNIQRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRFRSADDVE
KLVRMNWAQGIHKFFITDDNFARNKDWEAIFDRLIELKERDGIPLGLMIQVDTLCHKIPNFIEKSRRAGVTRVFIGLENV
NPDNLTAAKKNQNKITEYRKMLLAWKAQGIMTLAGYILGFPADTPESIRRDIAIIQEELPLDVIEFFILTPLPGSEDHQV
LWKKNVEMDSDLNIYDVEHVCTAHPKMSKQEWEDIYHEAWALYYSPDHMKTLLRRAVATGVPLARLVKVLVSFATTVPLE
NVHPLQSGLLRLKTPSERRPDLPRENPLVFWPRFAWDTFRKHASLAGTIIGLTISAFLISREAKSKTYMDQALTPVADDE
EETLSLFTKTAGGTAAVSHVRKVAELTRTAH

Sequences:

>Translated_591_residues
MSHVLEAARRRFQLILIKPSHYDDDGYVIRWWRAMIPSNSLAALYGIAAECAERKVLGPDTAIDITVIDETNTRIDFAGL
LAQFKRHDNFGMIALVGVQTNQYPRALDIARPFRDAGLPVSIGGFHVSGCLSMLDGKAVGLEACRDMGISMFAGEAEGRL
EMVLRDAASGELKPLYNFMNDLPGIGGTPVPFLPKDNIQRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRFRSADDVE
KLVRMNWAQGIHKFFITDDNFARNKDWEAIFDRLIELKERDGIPLGLMIQVDTLCHKIPNFIEKSRRAGVTRVFIGLENV
NPDNLTAAKKNQNKITEYRKMLLAWKAQGIMTLAGYILGFPADTPESIRRDIAIIQEELPLDVIEFFILTPLPGSEDHQV
LWKKNVEMDSDLNIYDVEHVCTAHPKMSKQEWEDIYHEAWALYYSPDHMKTLLRRAVATGVPLARLVKVLVSFATTVPLE
NVHPLQSGLLRLKTPSERRPDLPRENPLVFWPRFAWDTFRKHASLAGTIIGLTISAFLISREAKSKTYMDQALTPVADDE
EETLSLFTKTAGGTAAVSHVRKVAELTRTAH
>Mature_590_residues
SHVLEAARRRFQLILIKPSHYDDDGYVIRWWRAMIPSNSLAALYGIAAECAERKVLGPDTAIDITVIDETNTRIDFAGLL
AQFKRHDNFGMIALVGVQTNQYPRALDIARPFRDAGLPVSIGGFHVSGCLSMLDGKAVGLEACRDMGISMFAGEAEGRLE
MVLRDAASGELKPLYNFMNDLPGIGGTPVPFLPKDNIQRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRFRSADDVEK
LVRMNWAQGIHKFFITDDNFARNKDWEAIFDRLIELKERDGIPLGLMIQVDTLCHKIPNFIEKSRRAGVTRVFIGLENVN
PDNLTAAKKNQNKITEYRKMLLAWKAQGIMTLAGYILGFPADTPESIRRDIAIIQEELPLDVIEFFILTPLPGSEDHQVL
WKKNVEMDSDLNIYDVEHVCTAHPKMSKQEWEDIYHEAWALYYSPDHMKTLLRRAVATGVPLARLVKVLVSFATTVPLEN
VHPLQSGLLRLKTPSERRPDLPRENPLVFWPRFAWDTFRKHASLAGTIIGLTISAFLISREAKSKTYMDQALTPVADDEE
ETLSLFTKTAGGTAAVSHVRKVAELTRTAH

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 66338; Mature: 66207

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVLEAARRRFQLILIKPSHYDDDGYVIRWWRAMIPSNSLAALYGIAAECAERKVLGPD
CCHHHHHHHCCEEEEEECCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCC
TAIDITVIDETNTRIDFAGLLAQFKRHDNFGMIALVGVQTNQYPRALDIARPFRDAGLPV
CEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCE
SIGGFHVSGCLSMLDGKAVGLEACRDMGISMFAGEAEGRLEMVLRDAASGELKPLYNFMN
EECCEEHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHH
DLPGIGGTPVPFLPKDNIQRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRFRSADDVE
HCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHCCCHHHHH
KLVRMNWAQGIHKFFITDDNFARNKDWEAIFDRLIELKERDGIPLGLMIQVDTLCHKIPN
HHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHH
FIEKSRRAGVTRVFIGLENVNPDNLTAAKKNQNKITEYRKMLLAWKAQGIMTLAGYILGF
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
PADTPESIRRDIAIIQEELPLDVIEFFILTPLPGSEDHQVLWKKNVEMDSDLNIYDVEHV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEEHHHH
CTAHPKMSKQEWEDIYHEAWALYYSPDHMKTLLRRAVATGVPLARLVKVLVSFATTVPLE
HHCCCCCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
NVHPLQSGLLRLKTPSERRPDLPRENPLVFWPRFAWDTFRKHASLAGTIIGLTISAFLIS
CCCHHHHCCEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAKSKTYMDQALTPVADDEEETLSLFTKTAGGTAAVSHVRKVAELTRTAH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHVLEAARRRFQLILIKPSHYDDDGYVIRWWRAMIPSNSLAALYGIAAECAERKVLGPD
CHHHHHHHCCEEEEEECCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCC
TAIDITVIDETNTRIDFAGLLAQFKRHDNFGMIALVGVQTNQYPRALDIARPFRDAGLPV
CEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCE
SIGGFHVSGCLSMLDGKAVGLEACRDMGISMFAGEAEGRLEMVLRDAASGELKPLYNFMN
EECCEEHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHH
DLPGIGGTPVPFLPKDNIQRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRFRSADDVE
HCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHCCCHHHHH
KLVRMNWAQGIHKFFITDDNFARNKDWEAIFDRLIELKERDGIPLGLMIQVDTLCHKIPN
HHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHH
FIEKSRRAGVTRVFIGLENVNPDNLTAAKKNQNKITEYRKMLLAWKAQGIMTLAGYILGF
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
PADTPESIRRDIAIIQEELPLDVIEFFILTPLPGSEDHQVLWKKNVEMDSDLNIYDVEHV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEEHHHH
CTAHPKMSKQEWEDIYHEAWALYYSPDHMKTLLRRAVATGVPLARLVKVLVSFATTVPLE
HHCCCCCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
NVHPLQSGLLRLKTPSERRPDLPRENPLVFWPRFAWDTFRKHASLAGTIIGLTISAFLIS
CCCHHHHCCEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAKSKTYMDQALTPVADDEEETLSLFTKTAGGTAAVSHVRKVAELTRTAH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]