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The map label for this gene is xthA [H]

Identifier: 209549080

GI number: 209549080

Start: 1508786

End: 1509577

Strand: Reverse

Name: xthA [H]

Synonym: Rleg2_1479

Alternate gene names: 209549080

Gene position: 1509577-1508786 (Counterclockwise)

Preceding gene: 209549081

Following gene: 209549073

Centisome position: 33.27

GC content: 60.1

Gene sequence:

>792_bases
ATGAAGATCGCGACCTGGAACATCAACGGCGTCAAGGCGCGCATCGACAATCTCACGCAATGGCTCAAGGATTCAGATCC
GGATATCGTCTGCCTGCAGGAGATAAAGACGATCGACGAGGGTTTTCCCAGGCTGGAGATCGAGGCGCTCGGCTATCACG
TCGAAACACACGGGCAAAAGGGCTTCAACGGAGTGGCGATCCTCTCCAAGACTTCGCCTTCCGAAGTGAACCGCGGCCTG
CCCGGCGACCCGCTGGACGAACAGTCGCGTTTCCTCGAAGCCGTATTCACGCTGCCCGACACACGCATCCTCCGCGTCTG
CTGCATCTACCTACCGAACGGCAATCCTGTCGATACGGAGAAATATCCCTACAAGCTCGCCTGGATGGAGCGCCTGCGGA
GCTTTGCCGCCGAGCGGCTGGCCTATGAGGAGATGCTGGTGCTTGCCGGCGATTACAATGTCATCCCGGAACCGCACGAC
TGCTTCGATCCCAAGGTCTGGGAAAGTGATGCGCTGTTTCTGCCGCAGACGCGGGAGGCGTTCCGCCGGCTCGAAAATCT
CGGGCTGACGGATGCTGTGCGCGCGACGACGGATGCGACGCAGTTCTATTCCTTCTGGGATTATCAGGCCGGCGCCTGGC
CGAAGAACAACGGCATCCGCATCGACCATCTGCTGCTGTCGCCTGAGGCCGCCGACCGGATGACGTCGGCTGCGATCGAA
AAACATGTGCGGGCCTGGGAAAAGCCGTCCGACCACGTGCCTGTGATCGCCTATTTCGATTTCGCTGCCTGA

Upstream 100 bases:

>100_bases
ATCGAACCGGCCGAAGTTTTACTTCCGGCCGGTTTATCGGCTTGTTCGCCGGTGAAAGACCGCGATAAAAGAAGCGCACG
AAGCTAAACAGGACAGAGAG

Downstream 100 bases:

>100_bases
GTTTATTGCTGCTTAAAGCGCGTCGCGATGCATGTCGTTATCCCGGAACCGCTGCACACTTCCGGGCGACATGCATTAGT
CGTCTGAGCCGCTGGCGATG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKIATWNINGVKARIDNLTQWLKDSDPDIVCLQEIKTIDEGFPRLEIEALGYHVETHGQKGFNGVAILSKTSPSEVNRGL
PGDPLDEQSRFLEAVFTLPDTRILRVCCIYLPNGNPVDTEKYPYKLAWMERLRSFAAERLAYEEMLVLAGDYNVIPEPHD
CFDPKVWESDALFLPQTREAFRRLENLGLTDAVRATTDATQFYSFWDYQAGAWPKNNGIRIDHLLLSPEAADRMTSAAIE
KHVRAWEKPSDHVPVIAYFDFAA

Sequences:

>Translated_263_residues
MKIATWNINGVKARIDNLTQWLKDSDPDIVCLQEIKTIDEGFPRLEIEALGYHVETHGQKGFNGVAILSKTSPSEVNRGL
PGDPLDEQSRFLEAVFTLPDTRILRVCCIYLPNGNPVDTEKYPYKLAWMERLRSFAAERLAYEEMLVLAGDYNVIPEPHD
CFDPKVWESDALFLPQTREAFRRLENLGLTDAVRATTDATQFYSFWDYQAGAWPKNNGIRIDHLLLSPEAADRMTSAAIE
KHVRAWEKPSDHVPVIAYFDFAA
>Mature_263_residues
MKIATWNINGVKARIDNLTQWLKDSDPDIVCLQEIKTIDEGFPRLEIEALGYHVETHGQKGFNGVAILSKTSPSEVNRGL
PGDPLDEQSRFLEAVFTLPDTRILRVCCIYLPNGNPVDTEKYPYKLAWMERLRSFAAERLAYEEMLVLAGDYNVIPEPHD
CFDPKVWESDALFLPQTREAFRRLENLGLTDAVRATTDATQFYSFWDYQAGAWPKNNGIRIDHLLLSPEAADRMTSAAIE
KHVRAWEKPSDHVPVIAYFDFAA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=266, Percent_Identity=32.7067669172932, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI18375503, Length=266, Percent_Identity=32.7067669172932, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI18375501, Length=266, Percent_Identity=32.7067669172932, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=36.4312267657993, Blast_Score=151, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71989536, Length=273, Percent_Identity=30.4029304029304, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330655, Length=267, Percent_Identity=30.3370786516854, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17136678, Length=267, Percent_Identity=30.3370786516854, Blast_Score=101, Evalue=4e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29946; Mature: 29946

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNINGVKARIDNLTQWLKDSDPDIVCLQEIKTIDEGFPRLEIEALGYHVETHGQK
CEEEEECCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCEEEEEEEEEEEECCCCC
GFNGVAILSKTSPSEVNRGLPGDPLDEQSRFLEAVFTLPDTRILRVCCIYLPNGNPVDTE
CCCCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCCCC
KYPYKLAWMERLRSFAAERLAYEEMLVLAGDYNVIPEPHDCFDPKVWESDALFLPQTREA
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHH
FRRLENLGLTDAVRATTDATQFYSFWDYQAGAWPKNNGIRIDHLLLSPEAADRMTSAAIE
HHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHH
KHVRAWEKPSDHVPVIAYFDFAA
HHHHHHCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MKIATWNINGVKARIDNLTQWLKDSDPDIVCLQEIKTIDEGFPRLEIEALGYHVETHGQK
CEEEEECCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCEEEEEEEEEEEECCCCC
GFNGVAILSKTSPSEVNRGLPGDPLDEQSRFLEAVFTLPDTRILRVCCIYLPNGNPVDTE
CCCCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCCCC
KYPYKLAWMERLRSFAAERLAYEEMLVLAGDYNVIPEPHDCFDPKVWESDALFLPQTREA
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHH
FRRLENLGLTDAVRATTDATQFYSFWDYQAGAWPKNNGIRIDHLLLSPEAADRMTSAAIE
HHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHH
KHVRAWEKPSDHVPVIAYFDFAA
HHHHHHCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]