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The map label for this gene is pcm [H]

Identifier: 209549074

GI number: 209549074

Start: 1500709

End: 1501371

Strand: Direct

Name: pcm [H]

Synonym: Rleg2_1473

Alternate gene names: 209549074

Gene position: 1500709-1501371 (Clockwise)

Preceding gene: 209549071

Following gene: 209549075

Centisome position: 33.07

GC content: 62.29

Gene sequence:

>663_bases
ATGGATTTCGAAGCAGCGCGCGTAAAGATGGTCGACACCCAGGTTCGCACGACGGACGTTACCTCGCATTCCGTGCTGAC
AGCGTTTCTCACGGTCCCGCGTGAGGCATTCGTGCCGGAGAAGGCGAAGCTTCTGGCTTACATCGACAACGATGTCGAGA
TATCGGCCGCCGCGCCCGGAAAGCCGGCGCGCTTCCTGATGGAGGCATCGCCACTCGCCAAGCTGCTGCAGCTCGCCGCA
ATCACCAAGGATGATTTCGTCCTCGAAGTCGGTTGCGGCACCGGTTACACATCGGCGCTGCTGTCGATCATTGCCGGCTC
CGTCATCGCGCTCGAATGCGACGAGGCGCTGGCCGCCGAGGCGAAGACCCAGCTCGCCGGCTACGCCAAGGTCGAGGTGG
TCTCCGGATCGCTCGAAAAGGGCTACGCTGCCGGCGCTCCCTATGATCTGATTTTTATCAACGGTTCTGTTGAGGAGGTG
CCCGCAGCCCTTCTCGATCAATTGCGCGATGGCGGCCGTCTGATCACGGTCGAAGGTCACGGCAATGCCGCCCGCGCCAA
GGTGTTCGTCGCCGAGCGCGGCGCCGTTTCGGAAAACGTCTTCTTCAATGCCTCCGTCAAGCCGCTGCCGGGCTTCGCCA
AGGCGCGCGAATTCGTCTTCTGA

Upstream 100 bases:

>100_bases
GCCGGATTCTTTTCGCCGGCGGCTGCCAAGCCTTGAAAGCATCGGTGGCGGGGATTAAGAGACGAGAGACAGGAACCGCT
TTCAAGAGCGAGAGGACATG

Downstream 100 bases:

>100_bases
CACCGTTTGCCGGTGAACCAGCAACGGGCGCCACTGGCGCCCGTTTTTATTGCAGCGCAGCCGCGTCTTCTCCGACGCCC
AAAGGACGCTGTAACAGTTA

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MDFEAARVKMVDTQVRTTDVTSHSVLTAFLTVPREAFVPEKAKLLAYIDNDVEISAAAPGKPARFLMEASPLAKLLQLAA
ITKDDFVLEVGCGTGYTSALLSIIAGSVIALECDEALAAEAKTQLAGYAKVEVVSGSLEKGYAAGAPYDLIFINGSVEEV
PAALLDQLRDGGRLITVEGHGNAARAKVFVAERGAVSENVFFNASVKPLPGFAKAREFVF

Sequences:

>Translated_220_residues
MDFEAARVKMVDTQVRTTDVTSHSVLTAFLTVPREAFVPEKAKLLAYIDNDVEISAAAPGKPARFLMEASPLAKLLQLAA
ITKDDFVLEVGCGTGYTSALLSIIAGSVIALECDEALAAEAKTQLAGYAKVEVVSGSLEKGYAAGAPYDLIFINGSVEEV
PAALLDQLRDGGRLITVEGHGNAARAKVFVAERGAVSENVFFNASVKPLPGFAKAREFVF
>Mature_220_residues
MDFEAARVKMVDTQVRTTDVTSHSVLTAFLTVPREAFVPEKAKLLAYIDNDVEISAAAPGKPARFLMEASPLAKLLQLAA
ITKDDFVLEVGCGTGYTSALLSIIAGSVIALECDEALAAEAKTQLAGYAKVEVVSGSLEKGYAAGAPYDLIFINGSVEEV
PAALLDQLRDGGRLITVEGHGNAARAKVFVAERGAVSENVFFNASVKPLPGFAKAREFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=171, Percent_Identity=30.9941520467836, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23253; Mature: 23253

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEAARVKMVDTQVRTTDVTSHSVLTAFLTVPREAFVPEKAKLLAYIDNDVEISAAAPG
CCCCCCEEEEEECEEEEECCCHHHHHHHHHHCCHHHCCCCCCEEEEEECCCEEEEECCCC
KPARFLMEASPLAKLLQLAAITKDDFVLEVGCGTGYTSALLSIIAGSVIALECDEALAAE
CCHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCHHHHHH
AKTQLAGYAKVEVVSGSLEKGYAAGAPYDLIFINGSVEEVPAALLDQLRDGGRLITVEGH
HHHHHCCEEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECC
GNAARAKVFVAERGAVSENVFFNASVKPLPGFAKAREFVF
CCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MDFEAARVKMVDTQVRTTDVTSHSVLTAFLTVPREAFVPEKAKLLAYIDNDVEISAAAPG
CCCCCCEEEEEECEEEEECCCHHHHHHHHHHCCHHHCCCCCCEEEEEECCCEEEEECCCC
KPARFLMEASPLAKLLQLAAITKDDFVLEVGCGTGYTSALLSIIAGSVIALECDEALAAE
CCHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCHHHHHH
AKTQLAGYAKVEVVSGSLEKGYAAGAPYDLIFINGSVEEVPAALLDQLRDGGRLITVEGH
HHHHHCCEEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECC
GNAARAKVFVAERGAVSENVFFNASVKPLPGFAKAREFVF
CCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA