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The map label for this gene is phrA [H]

Identifier: 209549047

GI number: 209549047

Start: 1468402

End: 1469853

Strand: Reverse

Name: phrA [H]

Synonym: Rleg2_1444

Alternate gene names: 209549047

Gene position: 1469853-1468402 (Counterclockwise)

Preceding gene: 209549048

Following gene: 209549046

Centisome position: 32.39

GC content: 63.57

Gene sequence:

>1452_bases
TTGGCAAAGGACGCGGTAAAACCTGTCATTTTATGGTTTCGCAGGGATTTGCGCCTTGACGACAATCAGGCGCTGAATGC
CGCCCATCTCTCCGGGCGACCGATCATCCCCGTTTATATCAACGAGCCGGCGGCAGCCGGCACGGGTCCGCTCGGCGCTG
CGCAGGCCTGGTGGCTGCATCATTCGCTGGAAGCGCTTGATAGATCATTGCACGAGCGGCAGGGCGAGCTGGTGCTTGCC
AGCGGCGATGCGCTCGAGGTGCTCCGCGCCGTCATCAAAAAGAGCGGTGCCGAAGCCGTTTTCTGGAATCGACGCTACGA
TCCGTCGGGCATCTCGGTCGACACCCACATCAAGCAGGAATTGGAAAAACAGGCGATCGAGGCGAGAAGTTTCGGCGGCC
AGCTGCTGCACGAACCGTCCAGGCTGATGACCGGCAATGGCACGCCCTATCGGGTGTATACGCCATTCTGGCGCGCGCTG
GAGGGTGCGGGCGAAGCCGAGCCGCCGCTGGAGGCGCCGGCGAAGCTGCGGCTGGCGTCGCAGCGTCCGGCCTCGGAAAC
ATTGAAGAGCTGGAAACTGCTGCCGACGAAGCCGGACTGGGCGAAGGGCTTTGCCGACCTCTGGACGCCGGGCGAGCAAG
GCGCAAGAGAGAGGCTCAGCGCCTTCGTCGAGGACGAACTCAAGGGTTACAAGGAAAACCGCGACTATCCAGCGAAACCG
GCGACCTCAATGCTGTCGCCGCATCTGGCGCTCGGCGAGATCTCCCCTGCCCGCATCTGGGATGCGACGCGCGGTCTGTC
GAACCGGGTGCCGGCGGCCGACATCGTGCATTTCCGCAAGGAGATCGCCTGGCGGGAATTCTCCTACCACCTGCTTTTCC
ATTTCCCGCGTCTTGCCTCGGAGAACTGGAACGATCGTTTCGACGGCTTCAAATGGCGCAACGACGACGGCGATTTCGAG
GCGTGGCGCCGCGGCATGACCGGCTACCCGATCGTCGATGCCGGCATGCGCCAGCTCTGGCGCCACGGCTGGATGCACAA
TCGGGTGCGCATGATCGTCGCCTCCTTCCTCATCAAGGATCTGATGATCGACTGGCGCGACGGCGAGGCCTGGTTCCGCG
ACACGCTGGTCGATGCCGATCCCGCCAACAATGCGGCAAGCTGGCAATGGGTGGCGGGCTCGGGAGCCGACGCTTCACCC
TTCTTCCGCATCTTCAATCCGATGCTGCAGGGCGAGACTTTCGATCCCGACGGCGACTATGTCAGAGCCCATGTGCCGGA
GCTTCAACGGCTCGGGGCAAAATACATCCACCGGCCGTTCGAGGCGCCGAAGAGCGCGCTCGACGAGGCCGGCATCATCC
TCGGGCAGACCTATCCGAAACCGATCGTCGACCATGCCAGCGCCCGCGACCGGGCGCTCGCCGCTTACAAAGCCACAAAG
GACGCCGCATGA

Upstream 100 bases:

>100_bases
CCACCACGATGAAAAGCTTTCCCTTCGGATGAAACTTTTGATCCGGGCGTTCCGTACATCCGTAGCAGGGTGCAAAGAAC
AAGAGCAGGGAACGGCTTCA

Downstream 100 bases:

>100_bases
ACGCGCATGTCCGCCCCGCCTCGCGCCGGCTGAAGATCGGCGTCATCGGCTCCGGCATCTCCGGCGCCTCGGCGGCCTGG
GCGCTCGACCCCGTGCACGA

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAKDAVKPVILWFRRDLRLDDNQALNAAHLSGRPIIPVYINEPAAAGTGPLGAAQAWWLHHSLEALDRSLHERQGELVLA
SGDALEVLRAVIKKSGAEAVFWNRRYDPSGISVDTHIKQELEKQAIEARSFGGQLLHEPSRLMTGNGTPYRVYTPFWRAL
EGAGEAEPPLEAPAKLRLASQRPASETLKSWKLLPTKPDWAKGFADLWTPGEQGARERLSAFVEDELKGYKENRDYPAKP
ATSMLSPHLALGEISPARIWDATRGLSNRVPAADIVHFRKEIAWREFSYHLLFHFPRLASENWNDRFDGFKWRNDDGDFE
AWRRGMTGYPIVDAGMRQLWRHGWMHNRVRMIVASFLIKDLMIDWRDGEAWFRDTLVDADPANNAASWQWVAGSGADASP
FFRIFNPMLQGETFDPDGDYVRAHVPELQRLGAKYIHRPFEAPKSALDEAGIILGQTYPKPIVDHASARDRALAAYKATK
DAA

Sequences:

>Translated_483_residues
MAKDAVKPVILWFRRDLRLDDNQALNAAHLSGRPIIPVYINEPAAAGTGPLGAAQAWWLHHSLEALDRSLHERQGELVLA
SGDALEVLRAVIKKSGAEAVFWNRRYDPSGISVDTHIKQELEKQAIEARSFGGQLLHEPSRLMTGNGTPYRVYTPFWRAL
EGAGEAEPPLEAPAKLRLASQRPASETLKSWKLLPTKPDWAKGFADLWTPGEQGARERLSAFVEDELKGYKENRDYPAKP
ATSMLSPHLALGEISPARIWDATRGLSNRVPAADIVHFRKEIAWREFSYHLLFHFPRLASENWNDRFDGFKWRNDDGDFE
AWRRGMTGYPIVDAGMRQLWRHGWMHNRVRMIVASFLIKDLMIDWRDGEAWFRDTLVDADPANNAASWQWVAGSGADASP
FFRIFNPMLQGETFDPDGDYVRAHVPELQRLGAKYIHRPFEAPKSALDEAGIILGQTYPKPIVDHASARDRALAAYKATK
DAA
>Mature_482_residues
AKDAVKPVILWFRRDLRLDDNQALNAAHLSGRPIIPVYINEPAAAGTGPLGAAQAWWLHHSLEALDRSLHERQGELVLAS
GDALEVLRAVIKKSGAEAVFWNRRYDPSGISVDTHIKQELEKQAIEARSFGGQLLHEPSRLMTGNGTPYRVYTPFWRALE
GAGEAEPPLEAPAKLRLASQRPASETLKSWKLLPTKPDWAKGFADLWTPGEQGARERLSAFVEDELKGYKENRDYPAKPA
TSMLSPHLALGEISPARIWDATRGLSNRVPAADIVHFRKEIAWREFSYHLLFHFPRLASENWNDRFDGFKWRNDDGDFEA
WRRGMTGYPIVDAGMRQLWRHGWMHNRVRMIVASFLIKDLMIDWRDGEAWFRDTLVDADPANNAASWQWVAGSGADASPF
FRIFNPMLQGETFDPDGDYVRAHVPELQRLGAKYIHRPFEAPKSALDEAGIILGQTYPKPIVDHASARDRALAAYKATKD
AA

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=477, Percent_Identity=30.398322851153, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI4758072, Length=476, Percent_Identity=29.4117647058824, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI188536103, Length=440, Percent_Identity=29.3181818181818, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1786926, Length=485, Percent_Identity=36.4948453608247, Blast_Score=279, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6324962, Length=516, Percent_Identity=28.8759689922481, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17137248, Length=500, Percent_Identity=27.4, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24585455, Length=500, Percent_Identity=27.4, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24648152, Length=513, Percent_Identity=26.1208576998051, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54483; Mature: 54352

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDAVKPVILWFRRDLRLDDNQALNAAHLSGRPIIPVYINEPAAAGTGPLGAAQAWWLH
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
HSLEALDRSLHERQGELVLASGDALEVLRAVIKKSGAEAVFWNRRYDPSGISVDTHIKQE
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHH
LEKQAIEARSFGGQLLHEPSRLMTGNGTPYRVYTPFWRALEGAGEAEPPLEAPAKLRLAS
HHHHHHHHHHCCCHHHCCCCHHCCCCCCCEEEECHHHHHHHCCCCCCCCCCCCHHEEECC
QRPASETLKSWKLLPTKPDWAKGFADLWTPGEQGARERLSAFVEDELKGYKENRDYPAKP
CCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSMLSPHLALGEISPARIWDATRGLSNRVPAADIVHFRKEIAWREFSYHLLFHFPRLAS
HHHHHCCCHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENWNDRFDGFKWRNDDGDFEAWRRGMTGYPIVDAGMRQLWRHGWMHNRVRMIVASFLIKD
CCCCCCCCCCEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LMIDWRDGEAWFRDTLVDADPANNAASWQWVAGSGADASPFFRIFNPMLQGETFDPDGDY
HHCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCE
VRAHVPELQRLGAKYIHRPFEAPKSALDEAGIILGQTYPKPIVDHASARDRALAAYKATK
EEECCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCHHHHCCCHHHHHHHHHHHHC
DAA
CCC
>Mature Secondary Structure 
AKDAVKPVILWFRRDLRLDDNQALNAAHLSGRPIIPVYINEPAAAGTGPLGAAQAWWLH
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
HSLEALDRSLHERQGELVLASGDALEVLRAVIKKSGAEAVFWNRRYDPSGISVDTHIKQE
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHH
LEKQAIEARSFGGQLLHEPSRLMTGNGTPYRVYTPFWRALEGAGEAEPPLEAPAKLRLAS
HHHHHHHHHHCCCHHHCCCCHHCCCCCCCEEEECHHHHHHHCCCCCCCCCCCCHHEEECC
QRPASETLKSWKLLPTKPDWAKGFADLWTPGEQGARERLSAFVEDELKGYKENRDYPAKP
CCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSMLSPHLALGEISPARIWDATRGLSNRVPAADIVHFRKEIAWREFSYHLLFHFPRLAS
HHHHHCCCHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENWNDRFDGFKWRNDDGDFEAWRRGMTGYPIVDAGMRQLWRHGWMHNRVRMIVASFLIKD
CCCCCCCCCCEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LMIDWRDGEAWFRDTLVDADPANNAASWQWVAGSGADASPFFRIFNPMLQGETFDPDGDY
HHCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCE
VRAHVPELQRLGAKYIHRPFEAPKSALDEAGIILGQTYPKPIVDHASARDRALAAYKATK
EEECCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCHHHHCCCHHHHHHHHHHHHC
DAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]