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The map label for this gene is ubiE [H]

Identifier: 209548995

GI number: 209548995

Start: 1414225

End: 1415067

Strand: Direct

Name: ubiE [H]

Synonym: Rleg2_1392

Alternate gene names: 209548995

Gene position: 1414225-1415067 (Clockwise)

Preceding gene: 209548991

Following gene: 209548996

Centisome position: 31.16

GC content: 62.4

Gene sequence:

>843_bases
GTGACAATTTCTGCTGACATCGAAAACGGCGCTCCCGCCACCTCTGCCCCACCATTCGATGAAGGTATGGCCGAGCTTTA
CCAGACCCTCCTGGTGCCGATCCTCTTCGCGCCTTATGCGACCGAGATGGCGATCGCCGCCGACCAGTCGAAACCTGGAA
GCGTTCTCGAACTCGCTGCGGGGACGGGCGCCTTGACGCGCGCCCTGCGCGCGACGCTTGATCCGCCAACGGAAATTGTT
GCCACCGACCTCAGCCAGGCGATGATCGACATCGGCGCGCCGACGGTGACGATGTCGCGGACCCACTGGATGCACGCCGA
TGCGCAAAATCTTCCCTTCGCGCCGGAGATGTTCGATCTGGTCATCTGCCAGTTCGGCGCAATGTTCTTTCCCGACAAGG
TGAAGGCCTATAGCGAGGCAGAAAGAGTGTTGCGCAGCAAAGGCCGGTTTCTGTTTTCGACGTGGGATTCACTCTCGGTC
AACGACTTCGCGAGCTGTGTCGACGAATGCCTGGCAGGTCTCTTCCCATCCGACCCTCCGGATTTTCTCCGGCGCTTGCC
ATACTCCTACTTCGACCCCGGCGCCATCAAGGCGCAGATGTCTTCGGCAGGATTTGAGGCAGTGACCTGCGATCGGCTCG
AATTGACCTCCGTCGCCGCCAGCGCGCACGACGTCGCAGCCGCATTCTGCCAGGGCACGCTTCTGCGGGGCGAGATCGAA
TGGCGGGCGCCGGAACGCATGTCCGAAATCGTCGATGAGGTGGCCGAGCGCGTGGAAGCGCGGCACGGAACTCGTCCTAG
CGGCGGCATGAGCGCCTTGGTCGTCGCCGGCCGCGTTTCGTAA

Upstream 100 bases:

>100_bases
TCGGTCTGACAGGCCTCGGACCTAGGTCCGGGCCCCGTAGACGTCACCAGATTCAGTCATATTTCAGCACTGACTGGTTC
GGGGTTCTGGAGATTTGCGA

Downstream 100 bases:

>100_bases
TTCCCTCGGCCGAGTGCCGGCGCATTAATCATACAATTCCTACAATCATCCTCTCATTTTATAATCATTCTTAATTGGAA
CATGCTTCACTCGTCCGAAG

Product: type 11 methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTISADIENGAPATSAPPFDEGMAELYQTLLVPILFAPYATEMAIAADQSKPGSVLELAAGTGALTRALRATLDPPTEIV
ATDLSQAMIDIGAPTVTMSRTHWMHADAQNLPFAPEMFDLVICQFGAMFFPDKVKAYSEAERVLRSKGRFLFSTWDSLSV
NDFASCVDECLAGLFPSDPPDFLRRLPYSYFDPGAIKAQMSSAGFEAVTCDRLELTSVAASAHDVAAAFCQGTLLRGEIE
WRAPERMSEIVDEVAERVEARHGTRPSGGMSALVVAGRVS

Sequences:

>Translated_280_residues
MTISADIENGAPATSAPPFDEGMAELYQTLLVPILFAPYATEMAIAADQSKPGSVLELAAGTGALTRALRATLDPPTEIV
ATDLSQAMIDIGAPTVTMSRTHWMHADAQNLPFAPEMFDLVICQFGAMFFPDKVKAYSEAERVLRSKGRFLFSTWDSLSV
NDFASCVDECLAGLFPSDPPDFLRRLPYSYFDPGAIKAQMSSAGFEAVTCDRLELTSVAASAHDVAAAFCQGTLLRGEIE
WRAPERMSEIVDEVAERVEARHGTRPSGGMSALVVAGRVS
>Mature_279_residues
TISADIENGAPATSAPPFDEGMAELYQTLLVPILFAPYATEMAIAADQSKPGSVLELAAGTGALTRALRATLDPPTEIVA
TDLSQAMIDIGAPTVTMSRTHWMHADAQNLPFAPEMFDLVICQFGAMFFPDKVKAYSEAERVLRSKGRFLFSTWDSLSVN
DFASCVDECLAGLFPSDPPDFLRRLPYSYFDPGAIKAQMSSAGFEAVTCDRLELTSVAASAHDVAAAFCQGTLLRGEIEW
RAPERMSEIVDEVAERVEARHGTRPSGGMSALVVAGRVS

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=183, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30120; Mature: 29989

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISADIENGAPATSAPPFDEGMAELYQTLLVPILFAPYATEMAIAADQSKPGSVLELAA
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCEEEECC
GTGALTRALRATLDPPTEIVATDLSQAMIDIGAPTVTMSRTHWMHADAQNLPFAPEMFDL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCHHHHHH
VICQFGAMFFPDKVKAYSEAERVLRSKGRFLFSTWDSLSVNDFASCVDECLAGLFPSDPP
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCH
DFLRRLPYSYFDPGAIKAQMSSAGFEAVTCDRLELTSVAASAHDVAAAFCQGTLLRGEIE
HHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCHHEECEEC
WRAPERMSEIVDEVAERVEARHGTRPSGGMSALVVAGRVS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
TISADIENGAPATSAPPFDEGMAELYQTLLVPILFAPYATEMAIAADQSKPGSVLELAA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCEEEECC
GTGALTRALRATLDPPTEIVATDLSQAMIDIGAPTVTMSRTHWMHADAQNLPFAPEMFDL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCHHHHHH
VICQFGAMFFPDKVKAYSEAERVLRSKGRFLFSTWDSLSVNDFASCVDECLAGLFPSDPP
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCH
DFLRRLPYSYFDPGAIKAQMSSAGFEAVTCDRLELTSVAASAHDVAAAFCQGTLLRGEIE
HHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCHHEECEEC
WRAPERMSEIVDEVAERVEARHGTRPSGGMSALVVAGRVS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA