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The map label for this gene is ppnK [H]

Identifier: 209548911

GI number: 209548911

Start: 1336300

End: 1337073

Strand: Reverse

Name: ppnK [H]

Synonym: Rleg2_1308

Alternate gene names: 209548911

Gene position: 1337073-1336300 (Counterclockwise)

Preceding gene: 209548913

Following gene: 209548910

Centisome position: 29.46

GC content: 63.05

Gene sequence:

>774_bases
ATGGGCCGTTCATTTCAGACTCTTTCCTTTATCGCCTCGCCGACGACGGAGGCGCTCGCCGCGCGCGAGGAGTTGATTCG
TATCTATGGCGACGTGCCGGCCGACGATGCCGATGTCGTCGTCGCGCTCGGCGGCGATGGCTTCATGCTGCAGACGCTGC
ACAACACCATGAACTCCGGCAAGCTGGTCTACGGCATGAACCGCGGCTCGGTCGGCTTTCTGATGAACGACTACCGCAGT
GAACAGCTTCAGGAGCGTATCTGCGTCGCCGTCGAAAACGTCTTCCGGCCGTTGCAGATGACGACGGCCAACGCCGACGG
CACCAACTCAACGGCGCTCGCCATCAATGAGGTCTATCTTTTCCGCCAATCCTATCAGGCGGCGAATCTGAGGGTGACGG
TGGACGGGCGCGTGCGCCTGGAGGAACTGATCTGCGACGGGCTGATGGTGGCGACCCCTGCCGGGTCGACGGCCTATAAT
CTCTCCGCCCATGGCCCGATCCTGCCGCTCGAGGCGCCACTGCTCGCCATGACGCCGGTCAGCGCCTTCCGGCCGCGGCG
CTGGAGGGGCGCACTGCTTCCGAACAAGGTGACCGTCGATATCGACGTCCTCGAGCCCGTGAAGCGGCCGGTGAATGCGG
TGGCCGATAATACCGAGGTCAAATCGGTGCTGCATGTCCGCATTGCCCAGTCGGAGCATATGACGGCCCGCATCCTTTCC
GATCCCGACCGCTCCTGGTCCGACCGTATTCTCGCCGAGCAGTTCAAGGATTGA

Upstream 100 bases:

>100_bases
GCGGCGAGATCAGCCGTCCCCAAACGCTTGGCGATAGAAAACGTCCTTGTCGCACCAGCCCTTTATCCGTTAGCTAGAGC
AGCTTCACAAAGGGCATTGC

Downstream 100 bases:

>100_bases
GACGATGGCTGTGCGACGGATATTGATGACCACGGTTTTCCTCATCGGATGGTCGGCTGCAGCCTTGCCGGCGCAGGATG
CCATCCTGCCGGCATCGGTG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MGRSFQTLSFIASPTTEALAAREELIRIYGDVPADDADVVVALGGDGFMLQTLHNTMNSGKLVYGMNRGSVGFLMNDYRS
EQLQERICVAVENVFRPLQMTTANADGTNSTALAINEVYLFRQSYQAANLRVTVDGRVRLEELICDGLMVATPAGSTAYN
LSAHGPILPLEAPLLAMTPVSAFRPRRWRGALLPNKVTVDIDVLEPVKRPVNAVADNTEVKSVLHVRIAQSEHMTARILS
DPDRSWSDRILAEQFKD

Sequences:

>Translated_257_residues
MGRSFQTLSFIASPTTEALAAREELIRIYGDVPADDADVVVALGGDGFMLQTLHNTMNSGKLVYGMNRGSVGFLMNDYRS
EQLQERICVAVENVFRPLQMTTANADGTNSTALAINEVYLFRQSYQAANLRVTVDGRVRLEELICDGLMVATPAGSTAYN
LSAHGPILPLEAPLLAMTPVSAFRPRRWRGALLPNKVTVDIDVLEPVKRPVNAVADNTEVKSVLHVRIAQSEHMTARILS
DPDRSWSDRILAEQFKD
>Mature_256_residues
GRSFQTLSFIASPTTEALAAREELIRIYGDVPADDADVVVALGGDGFMLQTLHNTMNSGKLVYGMNRGSVGFLMNDYRSE
QLQERICVAVENVFRPLQMTTANADGTNSTALAINEVYLFRQSYQAANLRVTVDGRVRLEELICDGLMVATPAGSTAYNL
SAHGPILPLEAPLLAMTPVSAFRPRRWRGALLPNKVTVDIDVLEPVKRPVNAVADNTEVKSVLHVRIAQSEHMTARILSD
PDRSWSDRILAEQFKD

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=154, Percent_Identity=31.1688311688312, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325068, Length=188, Percent_Identity=31.9148936170213, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 28261; Mature: 28129

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRSFQTLSFIASPTTEALAAREELIRIYGDVPADDADVVVALGGDGFMLQTLHNTMNSG
CCCCCCHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEHHHHHHHCCCC
KLVYGMNRGSVGFLMNDYRSEQLQERICVAVENVFRPLQMTTANADGTNSTALAINEVYL
EEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHEE
FRQSYQAANLRVTVDGRVRLEELICDGLMVATPAGSTAYNLSAHGPILPLEAPLLAMTPV
EHHCCCCCEEEEEECCCEEHHHHHHCCEEEECCCCCCEEEECCCCCCCCCCCCEEEECCH
SAFRPRRWRGALLPNKVTVDIDVLEPVKRPVNAVADNTEVKSVLHVRIAQSEHMTARILS
HHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCEEEEEC
DPDRSWSDRILAEQFKD
CCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GRSFQTLSFIASPTTEALAAREELIRIYGDVPADDADVVVALGGDGFMLQTLHNTMNSG
CCCCCHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEHHHHHHHCCCC
KLVYGMNRGSVGFLMNDYRSEQLQERICVAVENVFRPLQMTTANADGTNSTALAINEVYL
EEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHEE
FRQSYQAANLRVTVDGRVRLEELICDGLMVATPAGSTAYNLSAHGPILPLEAPLLAMTPV
EHHCCCCCEEEEEECCCEEHHHHHHCCEEEECCCCCCEEEECCCCCCCCCCCCEEEECCH
SAFRPRRWRGALLPNKVTVDIDVLEPVKRPVNAVADNTEVKSVLHVRIAQSEHMTARILS
HHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCEEEEEC
DPDRSWSDRILAEQFKD
CCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]