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The map label for this gene is proS [H]

Identifier: 209548882

GI number: 209548882

Start: 1296935

End: 1298257

Strand: Direct

Name: proS [H]

Synonym: Rleg2_1279

Alternate gene names: 209548882

Gene position: 1296935-1298257 (Clockwise)

Preceding gene: 209548881

Following gene: 209548883

Centisome position: 28.58

GC content: 60.85

Gene sequence:

>1323_bases
ATGCGTCTGTCCCGCTATTTCATGCCCATCCTTAAGGAAAACCCCAAGGAGGCGGAAATCGTCTCCCACCGGCTGATGCT
GCGCGCCGGCATGATCCGCCAGCAGTCGCAGGGCATCTATTCCTGGCTGCCGCTCGGCAAGCGCGTGCTCGACAAAGTCA
ACGCCATTATCCGCGACGAGCAGAACCGGGCAGGTGCGATCGAGCTGTCGATGCCGACCTTGCAGTCGGCTGAACTCTGG
CAGGAAAGCGGCCGCTACGACGCCTATGGCAAGGAGATGCTGCGCATCAAGGACCGCCAGGATCGGCCGATGCTCTATGG
CCCCACTAACGAGGAGATGGTGACGGATATTTTCCGCTCCTCCGTCAAGTCCTACAAGGATCTGCCGCTCAATCTCTATC
ACATCCAGCTGAAGTTCCGCGACGAGATCCGCCCGCGCTTCGGCACCATGCGCTCGCGCGAATTCATGATGAAGGATGCC
TATTCCTTCGATCTGACGCGCGAAGGGGCGGAGCATTCCTACAACAAGATGTTCGCCGCCTATCTCAGGACCTTCGACCG
GCTCGGCTTGCGCGCCATTCCGATGCGCGCCGACACCGGCCCGATCGGCGGCAATCTCAGCCATGAATTCATCATCCTTG
CCGACACCGGTGAGTCCGAAGTCTTCTGCCACAAGGATTTCGTCGGCTTCGATATTCCCGACGACCGCACTGATTTCGAC
AGCGTCGACGGCCTGAAGGCGATCTTCGACAAGTGGACCTCGCTCTATGCGGCGACCTCGGAAATGCATGACGAGGCGGC
CTTCAACGCCGTTCCCGAAGGCGACCGCCTGTCGGCGCGCGGCATCGAAGTCGGCCATATCTTCTATTTCGGCACGAAAT
ATTCCGAGGCGATGGGCGCGAAAGTGCAGGGGCCCGACGGCAAGGAACACTTCGTTCACATGGGTTCCTACGGTATCGGC
CCGACACGCCTTGTTCCCGCCATCATCGAAGCATCGCATGACGACAACGGAATCATCTGGCCGGCGTCGGTCGCGCCCTT
CGATATCGTGGTGATCAACATGAAGGCGGGCGATCAGGCCTGCGACGATACCTGCGAGCTGATCTATGCCGCGCTGACCA
AGGCCGGCAAGGATGTGCTCTACGACGATACCGACGACCGGGCCGGCACGAAGTTCGCGACCGCCGACCTGATCGGCGTG
CCTTTCCAGATCATCGCTGGCCCGCGCGCGGTCGCCAACGGCGAAGTCGAGGTGAAGGACCGCAAGACCGGCGCCCGCGA
AACGATGACCATCGAAGCGGCGATCAACAGGTTCGTGGCTTAA

Upstream 100 bases:

>100_bases
GGTTTCCTTCCTGCCGCGAAGCGGCTATGAACGCTCCCGACATTAACCATTGTCTCTCGATTCCGCTTGCGGCGGGATCT
CAACTGCTTCGGAATCCGCC

Downstream 100 bases:

>100_bases
GGAAACGGAGGCGAAATGGCAGAGGCAGCAGTGGACCGGAGTTCGAAATCCGGCTTGGGTCCGGCTGGCAAGCCATTTTC
CACCTTCGAACGCCTCGTGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRLSRYFMPILKENPKEAEIVSHRLMLRAGMIRQQSQGIYSWLPLGKRVLDKVNAIIRDEQNRAGAIELSMPTLQSAELW
QESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMVTDIFRSSVKSYKDLPLNLYHIQLKFRDEIRPRFGTMRSREFMMKDA
YSFDLTREGAEHSYNKMFAAYLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFVGFDIPDDRTDFD
SVDGLKAIFDKWTSLYAATSEMHDEAAFNAVPEGDRLSARGIEVGHIFYFGTKYSEAMGAKVQGPDGKEHFVHMGSYGIG
PTRLVPAIIEASHDDNGIIWPASVAPFDIVVINMKAGDQACDDTCELIYAALTKAGKDVLYDDTDDRAGTKFATADLIGV
PFQIIAGPRAVANGEVEVKDRKTGARETMTIEAAINRFVA

Sequences:

>Translated_440_residues
MRLSRYFMPILKENPKEAEIVSHRLMLRAGMIRQQSQGIYSWLPLGKRVLDKVNAIIRDEQNRAGAIELSMPTLQSAELW
QESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMVTDIFRSSVKSYKDLPLNLYHIQLKFRDEIRPRFGTMRSREFMMKDA
YSFDLTREGAEHSYNKMFAAYLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFVGFDIPDDRTDFD
SVDGLKAIFDKWTSLYAATSEMHDEAAFNAVPEGDRLSARGIEVGHIFYFGTKYSEAMGAKVQGPDGKEHFVHMGSYGIG
PTRLVPAIIEASHDDNGIIWPASVAPFDIVVINMKAGDQACDDTCELIYAALTKAGKDVLYDDTDDRAGTKFATADLIGV
PFQIIAGPRAVANGEVEVKDRKTGARETMTIEAAINRFVA
>Mature_440_residues
MRLSRYFMPILKENPKEAEIVSHRLMLRAGMIRQQSQGIYSWLPLGKRVLDKVNAIIRDEQNRAGAIELSMPTLQSAELW
QESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMVTDIFRSSVKSYKDLPLNLYHIQLKFRDEIRPRFGTMRSREFMMKDA
YSFDLTREGAEHSYNKMFAAYLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFVGFDIPDDRTDFD
SVDGLKAIFDKWTSLYAATSEMHDEAAFNAVPEGDRLSARGIEVGHIFYFGTKYSEAMGAKVQGPDGKEHFVHMGSYGIG
PTRLVPAIIEASHDDNGIIWPASVAPFDIVVINMKAGDQACDDTCELIYAALTKAGKDVLYDDTDDRAGTKFATADLIGV
PFQIIAGPRAVANGEVEVKDRKTGARETMTIEAAINRFVA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=436, Percent_Identity=36.0091743119266, Blast_Score=265, Evalue=6e-71,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=58.3710407239819, Blast_Score=288, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI115532348, Length=438, Percent_Identity=32.1917808219178, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI193203271, Length=95, Percent_Identity=35.7894736842105, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=308, Percent_Identity=31.8181818181818, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24656200, Length=424, Percent_Identity=31.6037735849057, Blast_Score=221, Evalue=7e-58,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 49475; Mature: 49475

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFMPILKENPKEAEIVSHRLMLRAGMIRQQSQGIYSWLPLGKRVLDKVNAIIRDE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
QNRAGAIELSMPTLQSAELWQESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMVTDIFRS
CCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
SVKSYKDLPLNLYHIQLKFRDEIRPRFGTMRSREFMMKDAYSFDLTREGAEHSYNKMFAA
HHHHHHCCCEEEEEEEEEECCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFVGFDIPDDRTDFD
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
SVDGLKAIFDKWTSLYAATSEMHDEAAFNAVPEGDRLSARGIEVGHIFYFGTKYSEAMGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCC
KVQGPDGKEHFVHMGSYGIGPTRLVPAIIEASHDDNGIIWPASVAPFDIVVINMKAGDQA
EECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCCH
CDDTCELIYAALTKAGKDVLYDDTDDRAGTKFATADLIGVPFQIIAGPRAVANGEVEVKD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCCCEEECCCCEECCCEEEEEE
RKTGARETMTIEAAINRFVA
CCCCCCCEEEHHHHHHHHCC
>Mature Secondary Structure
MRLSRYFMPILKENPKEAEIVSHRLMLRAGMIRQQSQGIYSWLPLGKRVLDKVNAIIRDE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
QNRAGAIELSMPTLQSAELWQESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMVTDIFRS
CCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
SVKSYKDLPLNLYHIQLKFRDEIRPRFGTMRSREFMMKDAYSFDLTREGAEHSYNKMFAA
HHHHHHCCCEEEEEEEEEECCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFVGFDIPDDRTDFD
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
SVDGLKAIFDKWTSLYAATSEMHDEAAFNAVPEGDRLSARGIEVGHIFYFGTKYSEAMGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCC
KVQGPDGKEHFVHMGSYGIGPTRLVPAIIEASHDDNGIIWPASVAPFDIVVINMKAGDQA
EECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCCH
CDDTCELIYAALTKAGKDVLYDDTDDRAGTKFATADLIGVPFQIIAGPRAVANGEVEVKD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCCCEEECCCCEECCCEEEEEE
RKTGARETMTIEAAINRFVA
CCCCCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA