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The map label for this gene is paaF [C]

Identifier: 209548841

GI number: 209548841

Start: 1251734

End: 1252558

Strand: Direct

Name: paaF [C]

Synonym: Rleg2_1238

Alternate gene names: 209548841

Gene position: 1251734-1252558 (Clockwise)

Preceding gene: 209548840

Following gene: 209548844

Centisome position: 27.58

GC content: 63.03

Gene sequence:

>825_bases
ATGGCCGAAATCGTATCCTTCCCGGACGCAGGCAGGGCGGATGCGCTGCTGGTCCGCTCTCTGCGGGACGGAGTGTTGCG
GCTCGTGCTCAACAGCCCGCCGGCCAATGCGCTGTCGATCGCGCTTCTCGAAGCGCTGATGGCCGAATTCGATAAGGCCG
GCTCGGATGCGGATGTGCGCGTCGTCGTCATCGCCTCGACCGGCAATGTGTTTTCCGCCGGCCATGATCTCAAGGAACTC
ACCGCCCATCGCGCCGATCAAGATCGGGGGGCCGGTTTCTTCGAGACGGCCTTCCGGCTCTGCGCCGATCTGATGCTGAA
GATCGCGCATCTGCCGAAGCCTGTCATCGCCGAGGTCGACGGGCTGGCGACGGCGGCCGGCTGCCAGCTCGTCGCCTCCT
GCGATCTGGCGATCTGCACGGATACGTCGACATTCTGCACCCCGGGCGTCAATATCGGCCTGTTCTGCTCGACGCCGATG
GTGGCGGTTACCCGGGCTGCGCATCGCAAGCAGGCGATGGAGATGCTTTTGACCGGCGAGACGATCGACGCCTCCACAGC
CAAGGATTTCGGCCTCGTCAACCGCATCGTGCCGAAGCAGTATCTGGCGCAAGTCGTTGCCAAATATGCCGGAGTGATCG
CCAGCAAATCGCCGCTGACCCTGAAGATCGGCAAGGAAGCTTTCTACCGGCAGCTCGGACTGCCGGTCGAGGCAGCCTAT
GATTATGCCGCCAAAGTGATGGTCGACAACATGCTGACGCAGGATGCTGAGGAGGGGATCGGCGCTTTCCTCAACAAGCG
CACGCCGGAATGGACGGGCGAGTGA

Upstream 100 bases:

>100_bases
TAACGGCTGCCGCCTCAGCGGAAAGCGGTGATCCCGCTCGAAATTGCCGGCGCCTTGTGGCAATACCTCAGTCTGGAAAT
CGAGCGTAGGGAGCAGCATC

Downstream 100 bases:

>100_bases
TCACGCCAGTTTCAGAACCAGCCCGCCGATGGCGATAATGACGCCGGCGACGTAGCGCCAGAGGCTGGCCTTTTCCTTGA
AGACAAGAATCGAGATCACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAEIVSFPDAGRADALLVRSLRDGVLRLVLNSPPANALSIALLEALMAEFDKAGSDADVRVVVIASTGNVFSAGHDLKEL
TAHRADQDRGAGFFETAFRLCADLMLKIAHLPKPVIAEVDGLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPM
VAVTRAAHRKQAMEMLLTGETIDASTAKDFGLVNRIVPKQYLAQVVAKYAGVIASKSPLTLKIGKEAFYRQLGLPVEAAY
DYAAKVMVDNMLTQDAEEGIGAFLNKRTPEWTGE

Sequences:

>Translated_274_residues
MAEIVSFPDAGRADALLVRSLRDGVLRLVLNSPPANALSIALLEALMAEFDKAGSDADVRVVVIASTGNVFSAGHDLKEL
TAHRADQDRGAGFFETAFRLCADLMLKIAHLPKPVIAEVDGLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPM
VAVTRAAHRKQAMEMLLTGETIDASTAKDFGLVNRIVPKQYLAQVVAKYAGVIASKSPLTLKIGKEAFYRQLGLPVEAAY
DYAAKVMVDNMLTQDAEEGIGAFLNKRTPEWTGE
>Mature_273_residues
AEIVSFPDAGRADALLVRSLRDGVLRLVLNSPPANALSIALLEALMAEFDKAGSDADVRVVVIASTGNVFSAGHDLKELT
AHRADQDRGAGFFETAFRLCADLMLKIAHLPKPVIAEVDGLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMV
AVTRAAHRKQAMEMLLTGETIDASTAKDFGLVNRIVPKQYLAQVVAKYAGVIASKSPLTLKIGKEAFYRQLGLPVEAAYD
YAAKVMVDNMLTQDAEEGIGAFLNKRTPEWTGE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=46.6403162055336, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=30.6772908366534, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI70995211, Length=260, Percent_Identity=28.4615384615385, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260274832, Length=260, Percent_Identity=25.3846153846154, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI45643119, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502327, Length=277, Percent_Identity=27.4368231046931, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=32.8185328185328, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=30.7692307692308, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=29.4820717131474, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=26.0700389105058, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=25.9541984732824, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=268, Percent_Identity=41.7910447761194, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=30.1960784313725, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.1960784313725, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=26.7716535433071, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=267, Percent_Identity=25.4681647940075, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=28.8990825688073, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=212, Percent_Identity=25.9433962264151, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922422, Length=273, Percent_Identity=36.6300366300366, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=28.1124497991968, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=28.1124497991968, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19920382, Length=266, Percent_Identity=28.1954887218045, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=29.6992481203008, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654903, Length=179, Percent_Identity=27.3743016759777, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583165, Length=209, Percent_Identity=23.9234449760766, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45550169, Length=253, Percent_Identity=26.8774703557312, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29149; Mature: 29018

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIVSFPDAGRADALLVRSLRDGVLRLVLNSPPANALSIALLEALMAEFDKAGSDADVR
CCCCCCCCCCCCHHHHHHHHHHHHHHEEHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
VVVIASTGNVFSAGHDLKELTAHRADQDRGAGFFETAFRLCADLMLKIAHLPKPVIAEVD
EEEEEECCCEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMVAVTRAAHRKQAMEMLLTGE
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
TIDASTAKDFGLVNRIVPKQYLAQVVAKYAGVIASKSPLTLKIGKEAFYRQLGLPVEAAY
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCHHHHH
DYAAKVMVDNMLTQDAEEGIGAFLNKRTPEWTGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEIVSFPDAGRADALLVRSLRDGVLRLVLNSPPANALSIALLEALMAEFDKAGSDADVR
CCCCCCCCCCCHHHHHHHHHHHHHHEEHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
VVVIASTGNVFSAGHDLKELTAHRADQDRGAGFFETAFRLCADLMLKIAHLPKPVIAEVD
EEEEEECCCEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMVAVTRAAHRKQAMEMLLTGE
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
TIDASTAKDFGLVNRIVPKQYLAQVVAKYAGVIASKSPLTLKIGKEAFYRQLGLPVEAAY
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCHHHHH
DYAAKVMVDNMLTQDAEEGIGAFLNKRTPEWTGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA