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The map label for this gene is yfbT [H]

Identifier: 209548811

GI number: 209548811

Start: 1221711

End: 1222379

Strand: Direct

Name: yfbT [H]

Synonym: Rleg2_1208

Alternate gene names: 209548811

Gene position: 1221711-1222379 (Clockwise)

Preceding gene: 209548810

Following gene: 209548813

Centisome position: 26.92

GC content: 66.07

Gene sequence:

>669_bases
GTGCCCGCTCTCCACGATATCCTCGACACGTCCTATGACGCCTTTCTTTTCGACATGGACGGAACGCTGCTGAATTCCAT
CGCCGTCGTCGAACGCGTCTGGAGCGAGTGGGCAAGACGCCACGGTTTCGAGCCGGAAGTCTTCCTGAAGACCATCCACG
GCATCCGCGCCTCCGACGTCATCCGCGGGCTCGGCCTTGCCGGCGTCGATCCGGCCCATGAGGCGGACCTGCTGCTCGCC
GAGGAGATGGAGGATGTCTCGGGCATCGTCGAAATCCCCGGCGCCATTCGCTTCCTCTCCGCCATTGCAGACGGCAAATG
GGCGATCGTCACCTCGGCGCCGATCGAGCTTGCCAGGCGCCGCATGGCCGCCGCCGGCATCCCGATGCCGAAGGTCATCG
TCAGCGGCCAGGAGGTCAAATCCGGCAAGCCCAGCCCCGAAGGCTATCTGCTCGGCGCCAGCCGCCTCGGTGTCGATCCC
GCGAAATGCCTGGTCTTCGAAGACGCCGTCGCCGGCATTCTCGCCGGCGAGGCCGCCGGCGCCGATGTCGCCGTCATTAC
CGAAACCCACGCCACCCCCTTCGAAACCCCGCATTTCTCGATCGCCAACTACCAGGCATGGCAACCCGGCCACACCGCCG
AAGGCCGGCTGACGCTCGCGGCGATCTGA

Upstream 100 bases:

>100_bases
GTTGACGTCGGAGTCAACGACTGACGTTGACGCCGCCGCCGCTCTCCGCTACTCGCCTGTGCAGGGGTCGCCCCGGCCTC
GCACCCGCAAGGAACGCTCC

Downstream 100 bases:

>100_bases
TCAATCCGACAAACCGCCTCCTTTTTGTGGGAGGCGAGTTTTTCCGCTTGCATTGCACGCGCTGAAAAACTAAACGAAGC
AAGCCGTTTCGGCAGGTCGG

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MPALHDILDTSYDAFLFDMDGTLLNSIAVVERVWSEWARRHGFEPEVFLKTIHGIRASDVIRGLGLAGVDPAHEADLLLA
EEMEDVSGIVEIPGAIRFLSAIADGKWAIVTSAPIELARRRMAAAGIPMPKVIVSGQEVKSGKPSPEGYLLGASRLGVDP
AKCLVFEDAVAGILAGEAAGADVAVITETHATPFETPHFSIANYQAWQPGHTAEGRLTLAAI

Sequences:

>Translated_222_residues
MPALHDILDTSYDAFLFDMDGTLLNSIAVVERVWSEWARRHGFEPEVFLKTIHGIRASDVIRGLGLAGVDPAHEADLLLA
EEMEDVSGIVEIPGAIRFLSAIADGKWAIVTSAPIELARRRMAAAGIPMPKVIVSGQEVKSGKPSPEGYLLGASRLGVDP
AKCLVFEDAVAGILAGEAAGADVAVITETHATPFETPHFSIANYQAWQPGHTAEGRLTLAAI
>Mature_221_residues
PALHDILDTSYDAFLFDMDGTLLNSIAVVERVWSEWARRHGFEPEVFLKTIHGIRASDVIRGLGLAGVDPAHEADLLLAE
EMEDVSGIVEIPGAIRFLSAIADGKWAIVTSAPIELARRRMAAAGIPMPKVIVSGQEVKSGKPSPEGYLLGASRLGVDPA
KCLVFEDAVAGILAGEAAGADVAVITETHATPFETPHFSIANYQAWQPGHTAEGRLTLAAI

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI87082080, Length=190, Percent_Identity=45.7894736842105, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1789046, Length=181, Percent_Identity=30.3867403314917, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321833, Length=201, Percent_Identity=32.8358208955224, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321834, Length=201, Percent_Identity=32.3383084577114, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320905, Length=172, Percent_Identity=30.8139534883721, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322136, Length=181, Percent_Identity=30.939226519337, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23617; Mature: 23486

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALHDILDTSYDAFLFDMDGTLLNSIAVVERVWSEWARRHGFEPEVFLKTIHGIRASDV
CCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
IRGLGLAGVDPAHEADLLLAEEMEDVSGIVEIPGAIRFLSAIADGKWAIVTSAPIELARR
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
RMAAAGIPMPKVIVSGQEVKSGKPSPEGYLLGASRLGVDPAKCLVFEDAVAGILAGEAAG
HHHHCCCCCCCEEECCCHHCCCCCCCCCEEEEHHHCCCCHHHHEEEHHHHHHHHHCCCCC
ADVAVITETHATPFETPHFSIANYQAWQPGHTAEGRLTLAAI
CCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
PALHDILDTSYDAFLFDMDGTLLNSIAVVERVWSEWARRHGFEPEVFLKTIHGIRASDV
CHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
IRGLGLAGVDPAHEADLLLAEEMEDVSGIVEIPGAIRFLSAIADGKWAIVTSAPIELARR
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHH
RMAAAGIPMPKVIVSGQEVKSGKPSPEGYLLGASRLGVDPAKCLVFEDAVAGILAGEAAG
HHHHCCCCCCCEEECCCHHCCCCCCCCCEEEEHHHCCCCHHHHEEEHHHHHHHHHCCCCC
ADVAVITETHATPFETPHFSIANYQAWQPGHTAEGRLTLAAI
CCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]