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The map label for this gene is estB [H]

Identifier: 209548708

GI number: 209548708

Start: 1129267

End: 1130436

Strand: Reverse

Name: estB [H]

Synonym: Rleg2_1105

Alternate gene names: 209548708

Gene position: 1130436-1129267 (Counterclockwise)

Preceding gene: 209548710

Following gene: 209548706

Centisome position: 24.91

GC content: 65.13

Gene sequence:

>1170_bases
ATGAGTCTTCCCCTCGAAAAGGCAGAGGCCGACAGGCATTCATCGCTTGGCGCGGCTGTTGACGCGGCAATCACAAATGC
GCTCGACGAAAAGCGGCTGGTCGGGACGGTCGTGCTCGTCGCCCGCAACGGCGAGATCATTCACCGCCGCGCGGCAGGCT
TCGCCGACCGCGAAAACGGCCAGGCAATGCGCGAGGACGCGATCTTCAGGCTTGCCTCCATCACCAAGCCGATCGTCACC
ATCGCCGCGATGCGGCTCGTCGAGCAATCGAGGATCGGGCTCGACGACTCCGTGACCAGATGGCTGCCGGACTTCCGGCC
GAAGCTTCCCGACGGCGGCGAAGCCACCATTCGCATCCGTCACCTGCTGACACATACATCAGGCCTCGGCTACGGCTTCT
CCGAAGAGGACGGCGGTCCCTATATCAGCGCCGGCATTTCCGACGGCCTCGACCAGCCGGGACTGCGGCTCACCGAAAAT
CTGCGGCGGATCGCCAGCGTGCCGCTGCGTTTTGCGCCGGGCAGCGACTGGCAATATTCCCTTGCCATGGATGTTCTCGG
CGGCGTCATCGAGGCGGAAACCGGTGTCCCGTTGGGTGAGGCCGTTGCCGAACTGGTGACAAGACCGCTTGGCCTTGCCG
ACACGGCGTTTTCTGTTGGTGACCACAGCCGGCTTGCCGCCGCCTATATGGACGCTTCACCGGAACCGGCGCTCATGGGC
GAGAATGCGGTGGTGATGTCGCTGATGGGTCCCATCCGCTTCGCGCCGAACCGCATCTTCGACCCCGCCTCTTATCACTC
CGGCGGCGCCGGCATGGCGGGAACGGCCGGTGATGTTCTGGCCGTACTCGACACCATCCGTCGAGGCGGCGCACCGCTGC
TTTCGGCGGAAACCGTAGCGATGATGACGACCGACCAGGCCGGCGGGCACCGCCAGCAGCACGAACCCGGCTCCGGCTTT
GGCTTCGGCTGGTCTGTCATCACCGATCCGGCCGAGGCAGGCGTCCCCTTCCCCAAGGGCACGCTGAAATGGGGCGGCGT
CTACGGCCACAGCTGGTTCATCGACCCGGTCAACGGGCTGACCGTCGTTGCGCTGACGAACACGACGCTGGAGGGCATGT
GGGGCAAGTTCACGGTCGATCTTCGCGAGGCGATCTACGCGGCATTGTGA

Upstream 100 bases:

>100_bases
TTTTTACGTCGGCATTATTTTCCACAGTGATCACTCTAGAAATTCGAGACGGCTTGCTATATTCAGGAACGGTCATTGTG
ATTATAGAAGGTTTAATCTG

Downstream 100 bases:

>100_bases
ACAATCCGGATAAGCAAAAGGCCCGGCGACAGCGGTCGCCGGGCCTTTTGCATTCAAAAATGGGACGACTCAGTCCTTCT
TGACCGGCGGGATCGGCCGG

Product: beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSLPLEKAEADRHSSLGAAVDAAITNALDEKRLVGTVVLVARNGEIIHRRAAGFADRENGQAMREDAIFRLASITKPIVT
IAAMRLVEQSRIGLDDSVTRWLPDFRPKLPDGGEATIRIRHLLTHTSGLGYGFSEEDGGPYISAGISDGLDQPGLRLTEN
LRRIASVPLRFAPGSDWQYSLAMDVLGGVIEAETGVPLGEAVAELVTRPLGLADTAFSVGDHSRLAAAYMDASPEPALMG
ENAVVMSLMGPIRFAPNRIFDPASYHSGGAGMAGTAGDVLAVLDTIRRGGAPLLSAETVAMMTTDQAGGHRQQHEPGSGF
GFGWSVITDPAEAGVPFPKGTLKWGGVYGHSWFIDPVNGLTVVALTNTTLEGMWGKFTVDLREAIYAAL

Sequences:

>Translated_389_residues
MSLPLEKAEADRHSSLGAAVDAAITNALDEKRLVGTVVLVARNGEIIHRRAAGFADRENGQAMREDAIFRLASITKPIVT
IAAMRLVEQSRIGLDDSVTRWLPDFRPKLPDGGEATIRIRHLLTHTSGLGYGFSEEDGGPYISAGISDGLDQPGLRLTEN
LRRIASVPLRFAPGSDWQYSLAMDVLGGVIEAETGVPLGEAVAELVTRPLGLADTAFSVGDHSRLAAAYMDASPEPALMG
ENAVVMSLMGPIRFAPNRIFDPASYHSGGAGMAGTAGDVLAVLDTIRRGGAPLLSAETVAMMTTDQAGGHRQQHEPGSGF
GFGWSVITDPAEAGVPFPKGTLKWGGVYGHSWFIDPVNGLTVVALTNTTLEGMWGKFTVDLREAIYAAL
>Mature_388_residues
SLPLEKAEADRHSSLGAAVDAAITNALDEKRLVGTVVLVARNGEIIHRRAAGFADRENGQAMREDAIFRLASITKPIVTI
AAMRLVEQSRIGLDDSVTRWLPDFRPKLPDGGEATIRIRHLLTHTSGLGYGFSEEDGGPYISAGISDGLDQPGLRLTENL
RRIASVPLRFAPGSDWQYSLAMDVLGGVIEAETGVPLGEAVAELVTRPLGLADTAFSVGDHSRLAAAYMDASPEPALMGE
NAVVMSLMGPIRFAPNRIFDPASYHSGGAGMAGTAGDVLAVLDTIRRGGAPLLSAETVAMMTTDQAGGHRQQHEPGSGFG
FGWSVITDPAEAGVPFPKGTLKWGGVYGHSWFIDPVNGLTVVALTNTTLEGMWGKFTVDLREAIYAAL

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=357, Percent_Identity=29.1316526610644, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 41212; Mature: 41081

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPLEKAEADRHSSLGAAVDAAITNALDEKRLVGTVVLVARNGEIIHRRAAGFADRENG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEHHHCCCCCCCCC
QAMREDAIFRLASITKPIVTIAAMRLVEQSRIGLDDSVTRWLPDFRPKLPDGGEATIRIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEEE
HLLTHTSGLGYGFSEEDGGPYISAGISDGLDQPGLRLTENLRRIASVPLRFAPGSDWQYS
EHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCHH
LAMDVLGGVIEAETGVPLGEAVAELVTRPLGLADTAFSVGDHSRLAAAYMDASPEPALMG
HHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCC
ENAVVMSLMGPIRFAPNRIFDPASYHSGGAGMAGTAGDVLAVLDTIRRGGAPLLSAETVA
CCEEEEEHHCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCEEE
MMTTDQAGGHRQQHEPGSGFGFGWSVITDPAEAGVPFPKGTLKWGGVYGHSWFIDPVNGL
EEEECCCCCCCCCCCCCCCCCCCCCEECCHHHCCCCCCCCCEEECEEECCCEEECCCCCE
TVVALTNTTLEGMWGKFTVDLREAIYAAL
EEEEEECCCHHCCCCEEEHHHHHHHHHCC
>Mature Secondary Structure 
SLPLEKAEADRHSSLGAAVDAAITNALDEKRLVGTVVLVARNGEIIHRRAAGFADRENG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEHHHCCCCCCCCC
QAMREDAIFRLASITKPIVTIAAMRLVEQSRIGLDDSVTRWLPDFRPKLPDGGEATIRIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEEE
HLLTHTSGLGYGFSEEDGGPYISAGISDGLDQPGLRLTENLRRIASVPLRFAPGSDWQYS
EHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCHH
LAMDVLGGVIEAETGVPLGEAVAELVTRPLGLADTAFSVGDHSRLAAAYMDASPEPALMG
HHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCC
ENAVVMSLMGPIRFAPNRIFDPASYHSGGAGMAGTAGDVLAVLDTIRRGGAPLLSAETVA
CCEEEEEHHCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCEEE
MMTTDQAGGHRQQHEPGSGFGFGWSVITDPAEAGVPFPKGTLKWGGVYGHSWFIDPVNGL
EEEECCCCCCCCCCCCCCCCCCCCCEECCHHHCCCCCCCCCEEECEEECCCEEECCCCCE
TVVALTNTTLEGMWGKFTVDLREAIYAAL
EEEEEECCCHHCCCCEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]