Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 209548707

GI number: 209548707

Start: 1127407

End: 1129197

Strand: Direct

Name: aspS [H]

Synonym: Rleg2_1104

Alternate gene names: 209548707

Gene position: 1127407-1129197 (Clockwise)

Preceding gene: 209548702

Following gene: 209548709

Centisome position: 24.84

GC content: 63.99

Gene sequence:

>1791_bases
ATGCATCGCTATCGCAGCCACACATGCGCCGCCCTCCGCAAGACGGATGTCGGCTCGACCGTCCGTATCTCCGGCTGGGT
CCATCGCGTCCGCGACCATGGCGGCGTTCTCTTCATCGACCTTCGCGACCATTATGGCATCACCCAGGTCGTCGCCGATC
CGGACAGCCCGGCCTTCAAGATGGCCGAGACCGTGCGCGGCGAATGGGTCATCCGCATCGACGGCCTCGTCAAGGCCCGC
ACCGAAGATACGGTCAACAAGGCGATGGCGACCGGCGAAATCGAGCTCTACGCCCAGGAGATCGAAGTGCTCTCCGCTGC
CAAGGAATTGCCGCTTCCGGTGTTCGGCGAGCCGGACTATCCGGAAGACGTCCGCCTCAAATACCGCTTCCTCGATCTCC
GCCGCGAAACGCTGCATAAGAACATCGTCAAGCGCACCCAGGTGATCTCGGCGATGCGCCGCGAAATGGGCAATGTCGGC
TTCACCGAATATACGACGCCGATCCTGACGGCCTCCTCGCCGGAAGGCGCGCGCGACTTCCTCGTGCCGTCGCGCATCCA
TCCCGGCACCTTCTACGCCCTGCCGCAGGCGCCGCAGCAGTACAAGCAGCTGCTGATGGTTGCCGGCTTCGACCGCTACT
TCCAGATCGCGCCGTGCTTCCGCGATGAAGACCCGCGCGCCGACCGCCTGCCGGGCGAATTCTACCAGCTCGACCTCGAA
ATGAGCTTCGTCACCCAGGAAGACGTCTGGGACACGATGGGCCCGCTGATGACAAGCGTGTTCGAGGAGTTCGCCGAAGG
CAAGCCGGTCACCAAGGAGTGGCCGCGCATCCCCTATGACGAAGCGATCCGCAAATATGGTTCGGACAAGCCGGACCTGC
GCAACCCGATCGTCATGGAAGCGGTGACCGAGCATTTCGCCGGCTCCGGCTTCAAGGTCTTCGCCGGGATGATCGCCTCC
AACCCGAAGGTCCAGATCTGGGCGATCCCGGCAAAGACCGGCGGCTCGCGCGCCTTCTGCGACCGCATGAACGCCTGGGC
GCAGAGCCAGGGCCAGCCGGGTCTCGGCTATATCTTCTGGCGCAGCGAGAACGACAAGCTCGAAGGCGCCGGCCCGCTCG
CCAAGAACATCGGCGAGGAGCGCACCGATGCGATCCGCACCCAGCTCGGTCTCGGCGATGGCGACGCCTGCTTCTTCGTC
GCTGGCGAGCCGGAAAAATTCTACAAGTTTGCCGGCGAGGCGCGCACCAAGGCCGGCGAGGAACTGAACCTCGTCGACCG
CGACCGCTTCGAACTGTGCTGGATTGTCGACTTCCCCTTCTTCGAATGGAGCGAGGAAGACAAGAAGGTCGACTTCGCCC
ACAACCCGTTCTCGATGCCGCAGGGCGGCCTGGATGCGCTGCAGAATCAGGATCCGTTGACGATCAAGGCTTTCCAATAT
GACGCCGTCTGCAACGGCTTCGAGATCGCCTCGGGCTCGATCCGCAACCAGTCGCCGGAAACCATGGTCGCCGCCTTCGA
GAAGGTCGGCCTCAGCCAGCAGGACGTCGAGGATCGTTTCGGCGGCCTCTATCGCGCCTTCCAGTACGGAGCGCCGCCGC
ATGGTGGTGCTGCCTTCGGTATCGACCGCATCGTCATGCTGCTCGTCGGCGCCAAGAACCTGCGCGAAATCTCGGTATTC
CCGATGAACCAGCAGGCCCAGGATCTGCTGATGGGCGCGCCCAGCCCGGCAACCCCGACGCAGCTGCGCGAACTCTCGAT
CCGGCCGATCCCGCCGGTCAAGAAGGACTGA

Upstream 100 bases:

>100_bases
TCGGAGCGTCCATGCGCTTTTCCGCCCGATTTTCTTGTCGGCGCTTGAGGGTCTTTGAACCCTTGCTGCCGTCTTCAGCC
ACATGCCAGGATTTGAGATC

Downstream 100 bases:

>100_bases
GTCGTCCCATTTTTGAATGCAAAAGGCCCGGCGACCGCTGTCGCCGGGCCTTTTGCTTATCCGGATTGTTCACAATGCCG
CGTAGATCGCCTCGCGAAGA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MHRYRSHTCAALRKTDVGSTVRISGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFKMAETVRGEWVIRIDGLVKAR
TEDTVNKAMATGEIELYAQEIEVLSAAKELPLPVFGEPDYPEDVRLKYRFLDLRRETLHKNIVKRTQVISAMRREMGNVG
FTEYTTPILTASSPEGARDFLVPSRIHPGTFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEDVWDTMGPLMTSVFEEFAEGKPVTKEWPRIPYDEAIRKYGSDKPDLRNPIVMEAVTEHFAGSGFKVFAGMIAS
NPKVQIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWRSENDKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFV
AGEPEKFYKFAGEARTKAGEELNLVDRDRFELCWIVDFPFFEWSEEDKKVDFAHNPFSMPQGGLDALQNQDPLTIKAFQY
DAVCNGFEIASGSIRNQSPETMVAAFEKVGLSQQDVEDRFGGLYRAFQYGAPPHGGAAFGIDRIVMLLVGAKNLREISVF
PMNQQAQDLLMGAPSPATPTQLRELSIRPIPPVKKD

Sequences:

>Translated_596_residues
MHRYRSHTCAALRKTDVGSTVRISGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFKMAETVRGEWVIRIDGLVKAR
TEDTVNKAMATGEIELYAQEIEVLSAAKELPLPVFGEPDYPEDVRLKYRFLDLRRETLHKNIVKRTQVISAMRREMGNVG
FTEYTTPILTASSPEGARDFLVPSRIHPGTFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEDVWDTMGPLMTSVFEEFAEGKPVTKEWPRIPYDEAIRKYGSDKPDLRNPIVMEAVTEHFAGSGFKVFAGMIAS
NPKVQIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWRSENDKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFV
AGEPEKFYKFAGEARTKAGEELNLVDRDRFELCWIVDFPFFEWSEEDKKVDFAHNPFSMPQGGLDALQNQDPLTIKAFQY
DAVCNGFEIASGSIRNQSPETMVAAFEKVGLSQQDVEDRFGGLYRAFQYGAPPHGGAAFGIDRIVMLLVGAKNLREISVF
PMNQQAQDLLMGAPSPATPTQLRELSIRPIPPVKKD
>Mature_596_residues
MHRYRSHTCAALRKTDVGSTVRISGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFKMAETVRGEWVIRIDGLVKAR
TEDTVNKAMATGEIELYAQEIEVLSAAKELPLPVFGEPDYPEDVRLKYRFLDLRRETLHKNIVKRTQVISAMRREMGNVG
FTEYTTPILTASSPEGARDFLVPSRIHPGTFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEDVWDTMGPLMTSVFEEFAEGKPVTKEWPRIPYDEAIRKYGSDKPDLRNPIVMEAVTEHFAGSGFKVFAGMIAS
NPKVQIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWRSENDKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFV
AGEPEKFYKFAGEARTKAGEELNLVDRDRFELCWIVDFPFFEWSEEDKKVDFAHNPFSMPQGGLDALQNQDPLTIKAFQY
DAVCNGFEIASGSIRNQSPETMVAAFEKVGLSQQDVEDRFGGLYRAFQYGAPPHGGAAFGIDRIVMLLVGAKNLREISVF
PMNQQAQDLLMGAPSPATPTQLRELSIRPIPPVKKD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=603, Percent_Identity=33.8308457711443, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI45439306, Length=286, Percent_Identity=28.6713286713287, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI226491502, Length=259, Percent_Identity=26.2548262548263, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=596, Percent_Identity=40.9395973154362, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789256, Length=300, Percent_Identity=28.6666666666667, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790571, Length=301, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=32.3920265780731, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17551876, Length=300, Percent_Identity=27.6666666666667, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71984122, Length=296, Percent_Identity=26.6891891891892, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=250, Percent_Identity=26.8, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=250, Percent_Identity=26.8, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=250, Percent_Identity=26.8, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=654, Percent_Identity=28.1345565749235, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323011, Length=302, Percent_Identity=28.476821192053, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=251, Percent_Identity=25.0996015936255, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24584738, Length=601, Percent_Identity=33.4442595673877, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI17136276, Length=265, Percent_Identity=30.188679245283, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921528, Length=280, Percent_Identity=27.1428571428571, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66935; Mature: 66935

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRSHTCAALRKTDVGSTVRISGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFK
CCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHH
MAETVRGEWVIRIDGLVKARTEDTVNKAMATGEIELYAQEIEVLSAAKELPLPVFGEPDY
HHHHHCCCEEEEECCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDVRLKYRFLDLRRETLHKNIVKRTQVISAMRREMGNVGFTEYTTPILTASSPEGARDF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHC
LVPSRIHPGTFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVWDTMGPLMTSVFEEFAEGKPVTKEWPRIPYDEAIRKYGSDKPDLRNPIVME
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
AVTEHFAGSGFKVFAGMIASNPKVQIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFW
HHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
RSENDKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFVAGEPEKFYKFAGEARTKAGE
ECCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCC
ELNLVDRDRFELCWIVDFPFFEWSEEDKKVDFAHNPFSMPQGGLDALQNQDPLTIKAFQY
CCCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCEEEEEEE
DAVCNGFEIASGSIRNQSPETMVAAFEKVGLSQQDVEDRFGGLYRAFQYGAPPHGGAAFG
CHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
IDRIVMLLVGAKNLREISVFPMNQQAQDLLMGAPSPATPTQLRELSIRPIPPVKKD
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHRYRSHTCAALRKTDVGSTVRISGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFK
CCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHH
MAETVRGEWVIRIDGLVKARTEDTVNKAMATGEIELYAQEIEVLSAAKELPLPVFGEPDY
HHHHHCCCEEEEECCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDVRLKYRFLDLRRETLHKNIVKRTQVISAMRREMGNVGFTEYTTPILTASSPEGARDF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHC
LVPSRIHPGTFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVWDTMGPLMTSVFEEFAEGKPVTKEWPRIPYDEAIRKYGSDKPDLRNPIVME
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
AVTEHFAGSGFKVFAGMIASNPKVQIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFW
HHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
RSENDKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFVAGEPEKFYKFAGEARTKAGE
ECCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCC
ELNLVDRDRFELCWIVDFPFFEWSEEDKKVDFAHNPFSMPQGGLDALQNQDPLTIKAFQY
CCCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCEEEEEEE
DAVCNGFEIASGSIRNQSPETMVAAFEKVGLSQQDVEDRFGGLYRAFQYGAPPHGGAAFG
CHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
IDRIVMLLVGAKNLREISVFPMNQQAQDLLMGAPSPATPTQLRELSIRPIPPVKKD
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA