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The map label for this gene is ligE [H]

Identifier: 209548681

GI number: 209548681

Start: 1099285

End: 1099974

Strand: Direct

Name: ligE [H]

Synonym: Rleg2_1078

Alternate gene names: 209548681

Gene position: 1099285-1099974 (Clockwise)

Preceding gene: 209548674

Following gene: 209548682

Centisome position: 24.22

GC content: 63.48

Gene sequence:

>690_bases
ATGACCAGAGCCCTCTATTCCCTCTGCGGCGCCGATCAACAGCACCTCTTCTCGCCTCATTGCTGGAAGGCGGTGATGGC
ACTGGCGCATAAGGGGCTCGATTTCGAGGAAATCCCGACGACCTATGCCCGCATCCGCGCAATCGGCGGCGGCGTCTCGC
AGACCGTGCCCGTGCTCGACGACAACGGCCGGCTGATCCCCGACAGTTTCGACATCGCCCTCTATCTCGAAGAGGCCTAT
CCTGAGCGGCCGTCGCTGTTTAACGGCGAGGGCGGCGAGGCGCTGTCGCGCATGGTCGAGGGCTATTCGCAGATGATCAT
CCATCCGGCGATCACGCGGATTGCCCTTCTCGACATCCACGCCAATCTTGACGAGGGAGACAAGGCCTATTTCCGCGAGA
GCCGCGAAGCTCGTCTCGGCAAATCGTTCGAAGTCGTTGCTGCGGGTAGCGAGGCGGAGAAGGCCGCCTTCGGCGCCAAG
CTGGAGCCGCTCCGGCACATGCTGAAATTCCAGCCCTTCATCGGCGGGCAGGCGCCGCTCTTTGCCGATTATATCGTCTT
CGGCGCGCTGCAATGGCTGCGTGTCTGCACCGGCCTGACGATGCTGGCCGCCGACGATCCGGTCCTGGGCTGGTTCGAAC
GCTGCCTCGATCTCCATGAAAGCCGCGGCCGGACTGTGACAGCGGCGTGA

Upstream 100 bases:

>100_bases
TCCCTAGAAGCGGGAGTGATGACGCCATGAAATTTCCTGATTTGATCGCGGATGCTTTCTGTCGTGGAATGCTTGCAATT
GCGATTTCGAAAGGAATGTC

Downstream 100 bases:

>100_bases
AATTGCCGCCGACCTCTTGTTTTCGGGCGGTGGGGCGGGTAAAGACCGCCCACTTTTCCCGAGAAAACAGAGGATTTGAC
TATGGCGATTGAACGCACCT

Product: glutathione S-transferase domain

Products: NA

Alternate protein names: Beta-aryl ether cleaving enzyme [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTRALYSLCGADQQHLFSPHCWKAVMALAHKGLDFEEIPTTYARIRAIGGGVSQTVPVLDDNGRLIPDSFDIALYLEEAY
PERPSLFNGEGGEALSRMVEGYSQMIIHPAITRIALLDIHANLDEGDKAYFRESREARLGKSFEVVAAGSEAEKAAFGAK
LEPLRHMLKFQPFIGGQAPLFADYIVFGALQWLRVCTGLTMLAADDPVLGWFERCLDLHESRGRTVTAA

Sequences:

>Translated_229_residues
MTRALYSLCGADQQHLFSPHCWKAVMALAHKGLDFEEIPTTYARIRAIGGGVSQTVPVLDDNGRLIPDSFDIALYLEEAY
PERPSLFNGEGGEALSRMVEGYSQMIIHPAITRIALLDIHANLDEGDKAYFRESREARLGKSFEVVAAGSEAEKAAFGAK
LEPLRHMLKFQPFIGGQAPLFADYIVFGALQWLRVCTGLTMLAADDPVLGWFERCLDLHESRGRTVTAA
>Mature_228_residues
TRALYSLCGADQQHLFSPHCWKAVMALAHKGLDFEEIPTTYARIRAIGGGVSQTVPVLDDNGRLIPDSFDIALYLEEAYP
ERPSLFNGEGGEALSRMVEGYSQMIIHPAITRIALLDIHANLDEGDKAYFRESREARLGKSFEVVAAGSEAEKAAFGAKL
EPLRHMLKFQPFIGGQAPLFADYIVFGALQWLRVCTGLTMLAADDPVLGWFERCLDLHESRGRTVTAA

Specific function: Able to degrade various dimeric lignin compounds. Catalyzes the unique and reductive cleavage of arylglycerol-beta- aryl ether [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 25273; Mature: 25142

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRALYSLCGADQQHLFSPHCWKAVMALAHKGLDFEEIPTTYARIRAIGGGVSQTVPVLD
CCHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEC
DNGRLIPDSFDIALYLEEAYPERPSLFNGEGGEALSRMVEGYSQMIIHPAITRIALLDIH
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ANLDEGDKAYFRESREARLGKSFEVVAAGSEAEKAAFGAKLEPLRHMLKFQPFIGGQAPL
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCHH
FADYIVFGALQWLRVCTGLTMLAADDPVLGWFERCLDLHESRGRTVTAA
HHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure 
TRALYSLCGADQQHLFSPHCWKAVMALAHKGLDFEEIPTTYARIRAIGGGVSQTVPVLD
CHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEC
DNGRLIPDSFDIALYLEEAYPERPSLFNGEGGEALSRMVEGYSQMIIHPAITRIALLDIH
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ANLDEGDKAYFRESREARLGKSFEVVAAGSEAEKAAFGAKLEPLRHMLKFQPFIGGQAPL
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCHH
FADYIVFGALQWLRVCTGLTMLAADDPVLGWFERCLDLHESRGRTVTAA
HHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]