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The map label for this gene is lptD [H]

Identifier: 209548669

GI number: 209548669

Start: 1083696

End: 1086038

Strand: Reverse

Name: lptD [H]

Synonym: Rleg2_1066

Alternate gene names: 209548669

Gene position: 1086038-1083696 (Counterclockwise)

Preceding gene: 209548670

Following gene: 209548668

Centisome position: 23.93

GC content: 59.92

Gene sequence:

>2343_bases
GTGGCGGCAGGCGACCGCAAGTATTTTAGTAAACAGTTGGTTGCCCTGCTCGTCGGCGCGACTCTATGTTCCTATTTCGG
CAGTGTCCCGGTCTCCTACGGCCAGGCCAATACGTCCGCTCAAGATATCGGGAAGAATATTCCGGAAGGGGCCAAGCTTC
TGCTTTCGGCCAATGAACTCGTCTACAACCGTGACGCCGATCTGGTGTCGGCGGTCGGTGGCGTGCAGATCAACTATGCC
GGCTACAAAATGGTCGCGCAGAAGGTCGAGTACAATCAGAAGACCGGCCGGATGATGGCGCTCGGCAATGTCGAGCTGGT
CAGCCCGGACGGTAACCGCATCTATGCGGACAACCTTGACGTGACCGACAATTTCGCCGACGGGTTCCTGAACTCGCTGC
GCATCGAAACCTCCGACAATACGCGTATCGTCGCCGAAAGCGGCCAGCGTGTCGGCGGCACGATGATGATTCTCAACAAG
GGCGTCTACACCGCCTGCCTTCCCTGCGCCGAAGATCCGAAGCGCGCGCCTTTCTGGCAGGTCAAGGCCAAGCGGGTGAT
TCAAAACGGCGTGACCCACACGATCCGTCTGGAGCGGGCGCGCTTTGAGCTGCTCGGTTACCCCATTGCCTTCGTGCCTT
TCATCGAAGTCCCCGATAATACGGTGAAGCGCAAGTCGGGCTTTCTCTTCCCGACGATGAGCCTGTCGCAGAATCTCGGC
TTCGGTCTTTCCATTCCTTATTATTACGTGATCTCGCCGAGCATGGACGCGACGGTCACCACGACCGGCTACACCGCGCA
GGGCTTCCTCGTCGAAGGCGAATTCCGCCAGCGCTTCGAAAACGGCACGCATATCCTGCGCGTCGCCGGCATAGACCAGG
CAAAGCCGGACAATTTCAGCTCCGGCACCAGCGATGCCGAAGCCAGTCGGCGTGGCATGGTCGCCTCAAAGGCCGAATTC
CGTATCAATCCGCGCTGGACGTTCGGCTGGGACGTCATGGTGCAGAGCGACAACAATTTCTCGAAGACCTACAAGCTGAA
GGACTTCACCGGCACGGATCGCACCAACCAGATCTACCTGACCGGACTTGGGAAACGCAATTATTTCGACATGCGGGCGT
TCTATTTCGACGTCCAGGATGCCGATCGGAAGAACACGGCCGAAAAGCAGCAGGCGATCGTCTATCCGGCGCTAGACTAC
CACTATGTGGCGCCGCAGCCGCTGGCCGGCGGCGAATTGTCGGCTGATGTCAACCTGACGAATATTTCGCGCACCCATGA
CGACTTCTATACCGTCGACGGCTTCGACCGCTTCCGCGGCCTGAAGGGACAGACCTCGCGCCTGACGGCCGAACTGCAGT
GGAAGCGCACCTATGTCACGCCGACCGGCCTGGTGATCACGCCGCTTCTGGCCGCCCGCGGCGACGCCTTCGCGCTCAAC
ATGGACAGTCCGGTCAATGCCAACGGCGTTCCCTATGCCGGCAATTATCTCGACGACAATTCCGCCACCCGCTCGATGTT
CACCGCCGGGCTGGAGCTGCGCTATCCGATCCTGATGACGACCGAGAACAGCACCCATATTCTCGAACCGATCGCGCAGA
TCTATGCCCGCCCCGACGAGCAACTCGCCGGCCGGCTGCCGAACGAGGATGCGCAGAGCTTCGTCTTCGACGCCACCTCG
CTGTTCGATCGCGATAAGTTCTCCGGCTACGACCGCGTCGAAGGCGGCACCCGCGCCAATGTCGGCATCCAGTATACCGG
GACGTTCGACAGCGGCTACAAACTGCACGGCATCTTCGGCCAATCCTATCAGATTGCCGGCCAGAACTCCTTTGCCACCG
ATGATCTCGTCAATGTCGGTGCGGATTCGGGCCTGGAAACCGACCGTTCCGATTATGTCGGCCTTGGCGGCGTCGAAACA
CCATACGGCGTTTCCGTCGCGGCCTCCTACCGCCTCGACGAGAAGGATTTCGAGTTCCGCCGCGGCGACCTGACGACGGC
CTACCAGAACGACACCTTCCAGACGCAGGTGACCTACACCCATCTCAGCGCCCAGCCGGCCTATGGCTTTGCCGAGGACA
ACGACGAAATCCAGACCAGCAGCAAAGTCAAATTCAAGGATTATTGGGCGATCTTCGGCGGCATCGCCTGGGATCTGAAC
AATGATGTGCTCAGCCGCCGGACGCTCGGTCTCTCCTATGAGGACGAATGCACGATCTTCACGATCGCCTATACCGATAC
CCGGGACTCCGACGACGAGTCGGCCAGCGACTGGACGATCGGCGCAAGGCTGACCTTCCGGACGCTCGGCGACATCAAGA
TTGGCACAAACACCCTTGAGTGA

Upstream 100 bases:

>100_bases
GAAGCAGTGGAGTTGTGCCCCCCTTCGTGGCGACGTGGATACCGATGATCGTCGCGCTGGCCTTGGGTGCGACTATTCTG
CTTCATCAGGAGGACGGCTA

Downstream 100 bases:

>100_bases
CGGGCGGCCGGCGCAAACATGGCCTGAAGCCATTCTTTCGCCGTATGCCTGTGCAGGACATTGCCGCCAGTCGGTATCTG
AGGCATAGGGGTTTCGCCAC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 780; Mature: 779

Protein sequence:

>780_residues
MAAGDRKYFSKQLVALLVGATLCSYFGSVPVSYGQANTSAQDIGKNIPEGAKLLLSANELVYNRDADLVSAVGGVQINYA
GYKMVAQKVEYNQKTGRMMALGNVELVSPDGNRIYADNLDVTDNFADGFLNSLRIETSDNTRIVAESGQRVGGTMMILNK
GVYTACLPCAEDPKRAPFWQVKAKRVIQNGVTHTIRLERARFELLGYPIAFVPFIEVPDNTVKRKSGFLFPTMSLSQNLG
FGLSIPYYYVISPSMDATVTTTGYTAQGFLVEGEFRQRFENGTHILRVAGIDQAKPDNFSSGTSDAEASRRGMVASKAEF
RINPRWTFGWDVMVQSDNNFSKTYKLKDFTGTDRTNQIYLTGLGKRNYFDMRAFYFDVQDADRKNTAEKQQAIVYPALDY
HYVAPQPLAGGELSADVNLTNISRTHDDFYTVDGFDRFRGLKGQTSRLTAELQWKRTYVTPTGLVITPLLAARGDAFALN
MDSPVNANGVPYAGNYLDDNSATRSMFTAGLELRYPILMTTENSTHILEPIAQIYARPDEQLAGRLPNEDAQSFVFDATS
LFDRDKFSGYDRVEGGTRANVGIQYTGTFDSGYKLHGIFGQSYQIAGQNSFATDDLVNVGADSGLETDRSDYVGLGGVET
PYGVSVAASYRLDEKDFEFRRGDLTTAYQNDTFQTQVTYTHLSAQPAYGFAEDNDEIQTSSKVKFKDYWAIFGGIAWDLN
NDVLSRRTLGLSYEDECTIFTIAYTDTRDSDDESASDWTIGARLTFRTLGDIKIGTNTLE

Sequences:

>Translated_780_residues
MAAGDRKYFSKQLVALLVGATLCSYFGSVPVSYGQANTSAQDIGKNIPEGAKLLLSANELVYNRDADLVSAVGGVQINYA
GYKMVAQKVEYNQKTGRMMALGNVELVSPDGNRIYADNLDVTDNFADGFLNSLRIETSDNTRIVAESGQRVGGTMMILNK
GVYTACLPCAEDPKRAPFWQVKAKRVIQNGVTHTIRLERARFELLGYPIAFVPFIEVPDNTVKRKSGFLFPTMSLSQNLG
FGLSIPYYYVISPSMDATVTTTGYTAQGFLVEGEFRQRFENGTHILRVAGIDQAKPDNFSSGTSDAEASRRGMVASKAEF
RINPRWTFGWDVMVQSDNNFSKTYKLKDFTGTDRTNQIYLTGLGKRNYFDMRAFYFDVQDADRKNTAEKQQAIVYPALDY
HYVAPQPLAGGELSADVNLTNISRTHDDFYTVDGFDRFRGLKGQTSRLTAELQWKRTYVTPTGLVITPLLAARGDAFALN
MDSPVNANGVPYAGNYLDDNSATRSMFTAGLELRYPILMTTENSTHILEPIAQIYARPDEQLAGRLPNEDAQSFVFDATS
LFDRDKFSGYDRVEGGTRANVGIQYTGTFDSGYKLHGIFGQSYQIAGQNSFATDDLVNVGADSGLETDRSDYVGLGGVET
PYGVSVAASYRLDEKDFEFRRGDLTTAYQNDTFQTQVTYTHLSAQPAYGFAEDNDEIQTSSKVKFKDYWAIFGGIAWDLN
NDVLSRRTLGLSYEDECTIFTIAYTDTRDSDDESASDWTIGARLTFRTLGDIKIGTNTLE
>Mature_779_residues
AAGDRKYFSKQLVALLVGATLCSYFGSVPVSYGQANTSAQDIGKNIPEGAKLLLSANELVYNRDADLVSAVGGVQINYAG
YKMVAQKVEYNQKTGRMMALGNVELVSPDGNRIYADNLDVTDNFADGFLNSLRIETSDNTRIVAESGQRVGGTMMILNKG
VYTACLPCAEDPKRAPFWQVKAKRVIQNGVTHTIRLERARFELLGYPIAFVPFIEVPDNTVKRKSGFLFPTMSLSQNLGF
GLSIPYYYVISPSMDATVTTTGYTAQGFLVEGEFRQRFENGTHILRVAGIDQAKPDNFSSGTSDAEASRRGMVASKAEFR
INPRWTFGWDVMVQSDNNFSKTYKLKDFTGTDRTNQIYLTGLGKRNYFDMRAFYFDVQDADRKNTAEKQQAIVYPALDYH
YVAPQPLAGGELSADVNLTNISRTHDDFYTVDGFDRFRGLKGQTSRLTAELQWKRTYVTPTGLVITPLLAARGDAFALNM
DSPVNANGVPYAGNYLDDNSATRSMFTAGLELRYPILMTTENSTHILEPIAQIYARPDEQLAGRLPNEDAQSFVFDATSL
FDRDKFSGYDRVEGGTRANVGIQYTGTFDSGYKLHGIFGQSYQIAGQNSFATDDLVNVGADSGLETDRSDYVGLGGVETP
YGVSVAASYRLDEKDFEFRRGDLTTAYQNDTFQTQVTYTHLSAQPAYGFAEDNDEIQTSSKVKFKDYWAIFGGIAWDLNN
DVLSRRTLGLSYEDECTIFTIAYTDTRDSDDESASDWTIGARLTFRTLGDIKIGTNTLE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=206, Percent_Identity=26.6990291262136, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 86466; Mature: 86335

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGDRKYFSKQLVALLVGATLCSYFGSVPVSYGQANTSAQDIGKNIPEGAKLLLSANEL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHCCCCCCCEEEEECCCE
VYNRDADLVSAVGGVQINYAGYKMVAQKVEYNQKTGRMMALGNVELVSPDGNRIYADNLD
EEECCCHHHHHCCCEEEEECHHHHHHHHHHCCCCCCCEEEECCEEEECCCCCEEEECCCC
VTDNFADGFLNSLRIETSDNTRIVAESGQRVGGTMMILNKGVYTACLPCAEDPKRAPFWQ
CCCCHHHHHHHHEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHH
VKAKRVIQNGVTHTIRLERARFELLGYPIAFVPFIEVPDNTVKRKSGFLFPTMSLSQNLG
HHHHHHHHCCCEEEEEEECHHEEHHCCCEEEEEEEECCCCHHHHCCCCEEEEHHHHCCCC
FGLSIPYYYVISPSMDATVTTTGYTAQGFLVEGEFRQRFENGTHILRVAGIDQAKPDNFS
CCCCCCEEEEECCCCCCEEEECCEEEEEEEEECHHHHHHCCCCEEEEEECCCCCCCCCCC
SGTSDAEASRRGMVASKAEFRINPRWTFGWDVMVQSDNNFSKTYKLKDFTGTDRTNQIYL
CCCCCHHHHHCCCEECCCEEEECCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCEEEE
TGLGKRNYFDMRAFYFDVQDADRKNTAEKQQAIVYPALDYHYVAPQPLAGGELSADVNLT
EECCCCCEEEEEEEEEECCCCCCCCCCCHHHEEEEECCCEEEECCCCCCCCCEECCEEEE
NISRTHDDFYTVDGFDRFRGLKGQTSRLTAELQWKRTYVTPTGLVITPLLAARGDAFALN
ECCCCCCCEEEECCHHHHCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHCCCCEEEEE
MDSPVNANGVPYAGNYLDDNSATRSMFTAGLELRYPILMTTENSTHILEPIAQIYARPDE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCH
QLAGRLPNEDAQSFVFDATSLFDRDKFSGYDRVEGGTRANVGIQYTGTFDSGYKLHGIFG
HHHCCCCCCCHHHHEEEHHHHHCCCCCCCCCCCCCCCEECEEEEEEECCCCCEEEEEEEC
QSYQIAGQNSFATDDLVNVGADSGLETDRSDYVGLGGVETPYGVSVAASYRLDEKDFEFR
CEEEECCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCCCEE
RGDLTTAYQNDTFQTQVTYTHLSAQPAYGFAEDNDEIQTSSKVKFKDYWAIFGGIAWDLN
CCCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHEEEECCEEEECC
NDVLSRRTLGLSYEDECTIFTIAYTDTRDSDDESASDWTIGARLTFRTLGDIKIGTNTLE
CHHHHHEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEEEEECCEEECCCCCC
>Mature Secondary Structure 
AAGDRKYFSKQLVALLVGATLCSYFGSVPVSYGQANTSAQDIGKNIPEGAKLLLSANEL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHCCCCCCCEEEEECCCE
VYNRDADLVSAVGGVQINYAGYKMVAQKVEYNQKTGRMMALGNVELVSPDGNRIYADNLD
EEECCCHHHHHCCCEEEEECHHHHHHHHHHCCCCCCCEEEECCEEEECCCCCEEEECCCC
VTDNFADGFLNSLRIETSDNTRIVAESGQRVGGTMMILNKGVYTACLPCAEDPKRAPFWQ
CCCCHHHHHHHHEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHH
VKAKRVIQNGVTHTIRLERARFELLGYPIAFVPFIEVPDNTVKRKSGFLFPTMSLSQNLG
HHHHHHHHCCCEEEEEEECHHEEHHCCCEEEEEEEECCCCHHHHCCCCEEEEHHHHCCCC
FGLSIPYYYVISPSMDATVTTTGYTAQGFLVEGEFRQRFENGTHILRVAGIDQAKPDNFS
CCCCCCEEEEECCCCCCEEEECCEEEEEEEEECHHHHHHCCCCEEEEEECCCCCCCCCCC
SGTSDAEASRRGMVASKAEFRINPRWTFGWDVMVQSDNNFSKTYKLKDFTGTDRTNQIYL
CCCCCHHHHHCCCEECCCEEEECCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCEEEE
TGLGKRNYFDMRAFYFDVQDADRKNTAEKQQAIVYPALDYHYVAPQPLAGGELSADVNLT
EECCCCCEEEEEEEEEECCCCCCCCCCCHHHEEEEECCCEEEECCCCCCCCCEECCEEEE
NISRTHDDFYTVDGFDRFRGLKGQTSRLTAELQWKRTYVTPTGLVITPLLAARGDAFALN
ECCCCCCCEEEECCHHHHCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHCCCCEEEEE
MDSPVNANGVPYAGNYLDDNSATRSMFTAGLELRYPILMTTENSTHILEPIAQIYARPDE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCH
QLAGRLPNEDAQSFVFDATSLFDRDKFSGYDRVEGGTRANVGIQYTGTFDSGYKLHGIFG
HHHCCCCCCCHHHHEEEHHHHHCCCCCCCCCCCCCCCEECEEEEEEECCCCCEEEEEEEC
QSYQIAGQNSFATDDLVNVGADSGLETDRSDYVGLGGVETPYGVSVAASYRLDEKDFEFR
CEEEECCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCCCEE
RGDLTTAYQNDTFQTQVTYTHLSAQPAYGFAEDNDEIQTSSKVKFKDYWAIFGGIAWDLN
CCCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHEEEECCEEEECC
NDVLSRRTLGLSYEDECTIFTIAYTDTRDSDDESASDWTIGARLTFRTLGDIKIGTNTLE
CHHHHHEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEEEEECCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA