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The map label for this gene is rhtB [H]

Identifier: 209548648

GI number: 209548648

Start: 1064193

End: 1064828

Strand: Direct

Name: rhtB [H]

Synonym: Rleg2_1045

Alternate gene names: 209548648

Gene position: 1064193-1064828 (Clockwise)

Preceding gene: 209548643

Following gene: 209548650

Centisome position: 23.45

GC content: 63.21

Gene sequence:

>636_bases
ATGTTCGATTATTCGCTCGCGCACTGGTTTGCATTTCTCTCGGCGGCGGTTCTGCTCAACCTGTCGCCCGGCCCCGACAT
CGCTTTCATCCTCGCTCACACGATGAAAGGTGGAAAACGCGCCGGTTTTTCCGCGCTGTTCGGCGTCTGGTCCGGCGCCT
GCCTGCATGTTCTGATGGCAGCGCTCGGCCTTTCCGCCGTGCTCGCCGCTTCCTCAGTCGCTTTCTCCGCGGTCAAATGG
GCGGGCGCCGCCTATCTCGTCTGGCTGGGCATCCAGGCTCTGCGCGCCGGCGGCGGCGATGGCCTGATAAAGGCTGCCGG
TGAAAAATTGCCGGCGTCGAAAATCTACCGGCAGGGCATTCTGGTGTCGCTGCTCAATCCGAAGGTGGCGATTTTCTTCC
TTGCCTTCCTGCCGCAATTCGTCGTCGAAGGGGCAGGGCCGGCATGGGCGCAGTTGATGCTGCATGGCGGGCTCATCATC
GTCGTCGCCGCCTTCATCGAACCGCCGCTCGTCCTTCTCGGAGGGCGGCTTGCCGATGCGCTCAGGCATAATCAAAAAAT
CGGGCTATGGCTCGATCGCGGCCTCGGCGCGCTTTTCCTGGCGCTCGGTGTCCGGCTGGCGCTCAGCAGCCGCTGA

Upstream 100 bases:

>100_bases
TGCCTGAGATAATGGCTATCGCGACGGATCAAAACTAATGATTTTCCTATCAGCGCCTCCGTCCCTATCCTTTGCCCCTG
CTCATCCTGAACGGTGGAAG

Downstream 100 bases:

>100_bases
CGGGGAAGCTATTCCTCGTCGGCGACATAGCGCCGTTCGTGGCGCAGCCCCATGCTGGTCAGGATTTCATATCCGATCGT
GCCCGCCGCCCGCGCCACGT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MFDYSLAHWFAFLSAAVLLNLSPGPDIAFILAHTMKGGKRAGFSALFGVWSGACLHVLMAALGLSAVLAASSVAFSAVKW
AGAAYLVWLGIQALRAGGGDGLIKAAGEKLPASKIYRQGILVSLLNPKVAIFFLAFLPQFVVEGAGPAWAQLMLHGGLII
VVAAFIEPPLVLLGGRLADALRHNQKIGLWLDRGLGALFLALGVRLALSSR

Sequences:

>Translated_211_residues
MFDYSLAHWFAFLSAAVLLNLSPGPDIAFILAHTMKGGKRAGFSALFGVWSGACLHVLMAALGLSAVLAASSVAFSAVKW
AGAAYLVWLGIQALRAGGGDGLIKAAGEKLPASKIYRQGILVSLLNPKVAIFFLAFLPQFVVEGAGPAWAQLMLHGGLII
VVAAFIEPPLVLLGGRLADALRHNQKIGLWLDRGLGALFLALGVRLALSSR
>Mature_211_residues
MFDYSLAHWFAFLSAAVLLNLSPGPDIAFILAHTMKGGKRAGFSALFGVWSGACLHVLMAALGLSAVLAASSVAFSAVKW
AGAAYLVWLGIQALRAGGGDGLIKAAGEKLPASKIYRQGILVSLLNPKVAIFFLAFLPQFVVEGAGPAWAQLMLHGGLII
VVAAFIEPPLVLLGGRLADALRHNQKIGLWLDRGLGALFLALGVRLALSSR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=213, Percent_Identity=31.924882629108, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI1788099, Length=205, Percent_Identity=23.9024390243902, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI48994978, Length=192, Percent_Identity=26.0416666666667, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22100; Mature: 22100

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYSLAHWFAFLSAAVLLNLSPGPDIAFILAHTMKGGKRAGFSALFGVWSGACLHVLMA
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALGLSAVLAASSVAFSAVKWAGAAYLVWLGIQALRAGGGDGLIKAAGEKLPASKIYRQGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHH
LVSLLNPKVAIFFLAFLPQFVVEGAGPAWAQLMLHGGLIIVVAAFIEPPLVLLGGRLADA
HHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
LRHNQKIGLWLDRGLGALFLALGVRLALSSR
HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDYSLAHWFAFLSAAVLLNLSPGPDIAFILAHTMKGGKRAGFSALFGVWSGACLHVLMA
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALGLSAVLAASSVAFSAVKWAGAAYLVWLGIQALRAGGGDGLIKAAGEKLPASKIYRQGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHH
LVSLLNPKVAIFFLAFLPQFVVEGAGPAWAQLMLHGGLIIVVAAFIEPPLVLLGGRLADA
HHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
LRHNQKIGLWLDRGLGALFLALGVRLALSSR
HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]