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The map label for this gene is argD [C]

Identifier: 209548555

GI number: 209548555

Start: 975767

End: 977134

Strand: Direct

Name: argD [C]

Synonym: Rleg2_0952

Alternate gene names: 209548555

Gene position: 975767-977134 (Clockwise)

Preceding gene: 209548553

Following gene: 209548560

Centisome position: 21.5

GC content: 62.94

Gene sequence:

>1368_bases
ATGTCCGATACCTACCCGCCCTCGAACCTTGCCGCGATCGACGCCGCACACCATCTGCATCCCTTCGCCGACATGAAGAA
GCTGAATGCCGATGGCGCGCGCATCATCCAGCGCGGCGAGGGCGTCCACATCTTCGACAATCACGGCAGGAAATATCTCG
ACGGCTTCGCCGGCCTCTGGTGCGTCAATATCGGTTATGGCCGCCGGGAGATCACTAACGCCGTCGCAAGGCAGATGAAC
GAACTGCCCTATTACAACACCTTCTTCGGCACGAGTTCGGCGCCGGCGACACTGCTGGCGCAGAAGGTCACCTCACATGC
CGGAGAACGTTTCAATCATATCTTCTTCACCGGCTCCGGATCGGAGGCGAACGACACCTGGTTCCGCATGGCCCGCGTTT
ACTGGAGCGCCGTCGGCAAGCCGTCGAAGAAGATCGTGATCGCCAGGAAGAACGGCTATCACGGTTCCACCGTCGCCGGC
GCCAGCCTTTGCGGCATGAAATATATGCACGAGCAGGGCGACCTGCCGATCCCAGGCATCGTCCATATCGGCCAACCCTA
TTGGTATGGCGAGGGCGGCGATCTCTCGCCTGATGAATTCGGCCTCAAGGTCGCCCGCGAGCTCGAAACGAAGATCGACG
AGTTGGGCGAGGAGAATGTCGCAGCCTTCGTCGCCGAGCCGGTGCAGGGGGCTGCCGGCGTCATCATCCCACCGGAGACC
TACTGGCCGGAGATCGACCGCATCTGCAAGGCGCGTGATATTCTGCTGGTGACCGACGAGGTGATCTGCGGTTTCGGCCG
GTTGGGTGCCTGGTTCGGCCACCAGCATTTCGGCGTCGAGCCGGATCTCGCGCCGATCGCCAAGGGGCTATCCTCCGGCT
ACCTGCCGATCGGCGGCGTCCTCGTCAGCGACCGCATCGCCGACGTGCTGATCAACGAGGTCGGCGAGTTCAACCACGGC
TTCACCTATTCCGGCCATCCGGTCTGCGCGGCCGCCGCCCTGGAAAATCTGCGCATCATCGAGGAGGAGAAACTGGTCGA
ACGCGTGCGTGACGATATTGGCCCCTATTTCGGCAAGGCCTGGGCAGCACTCGCCGACCATGACCTGGTCGGCGAAGCCG
AGAGTGTCGGCCTGATGGGCGGCCTGCAGCTTGCAGCCGACAAGAGCACGCGCCGCCGTTTCGACAAACCCGATCAGATC
GGTGCCCTGGTACGCAACCACGCGCTCGCCAACGGCCTCGTGCTGCGCGCCACCGGCGATCGCATGCTGGCCTCGCCACC
GCTCGTCATCAGCCATGCGGAGGTGGACGAGATGGCAAGGATTACCAGGCTGGCGCTGGATTTGGCCTGGAAGGAGCTGA
AATCGTGA

Upstream 100 bases:

>100_bases
TCCATCCTGTCGCTCGCCATGACCTTGAAAATCCTCCGGGACAAGGCCAAGCTCCTATCCCATCGATAGCAGCCTTTCAA
TCCGCGAGGGTCTTCACCTT

Downstream 100 bases:

>100_bases
CTGCGCTCAGCCTGGCTGGTTGATCCAAGCATAGCCGAATCGGTAGCGGTCCTCGCCGCGCTGATAGAAATAGGGCACGT
CGATCACCAATCCCTCAGGC

Product: aminotransferase

Products: N-Acetyl-L-glutamate 5-semialdehyde; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSDTYPPSNLAAIDAAHHLHPFADMKKLNADGARIIQRGEGVHIFDNHGRKYLDGFAGLWCVNIGYGRREITNAVARQMN
ELPYYNTFFGTSSAPATLLAQKVTSHAGERFNHIFFTGSGSEANDTWFRMARVYWSAVGKPSKKIVIARKNGYHGSTVAG
ASLCGMKYMHEQGDLPIPGIVHIGQPYWYGEGGDLSPDEFGLKVARELETKIDELGEENVAAFVAEPVQGAAGVIIPPET
YWPEIDRICKARDILLVTDEVICGFGRLGAWFGHQHFGVEPDLAPIAKGLSSGYLPIGGVLVSDRIADVLINEVGEFNHG
FTYSGHPVCAAAALENLRIIEEEKLVERVRDDIGPYFGKAWAALADHDLVGEAESVGLMGGLQLAADKSTRRRFDKPDQI
GALVRNHALANGLVLRATGDRMLASPPLVISHAEVDEMARITRLALDLAWKELKS

Sequences:

>Translated_455_residues
MSDTYPPSNLAAIDAAHHLHPFADMKKLNADGARIIQRGEGVHIFDNHGRKYLDGFAGLWCVNIGYGRREITNAVARQMN
ELPYYNTFFGTSSAPATLLAQKVTSHAGERFNHIFFTGSGSEANDTWFRMARVYWSAVGKPSKKIVIARKNGYHGSTVAG
ASLCGMKYMHEQGDLPIPGIVHIGQPYWYGEGGDLSPDEFGLKVARELETKIDELGEENVAAFVAEPVQGAAGVIIPPET
YWPEIDRICKARDILLVTDEVICGFGRLGAWFGHQHFGVEPDLAPIAKGLSSGYLPIGGVLVSDRIADVLINEVGEFNHG
FTYSGHPVCAAAALENLRIIEEEKLVERVRDDIGPYFGKAWAALADHDLVGEAESVGLMGGLQLAADKSTRRRFDKPDQI
GALVRNHALANGLVLRATGDRMLASPPLVISHAEVDEMARITRLALDLAWKELKS
>Mature_454_residues
SDTYPPSNLAAIDAAHHLHPFADMKKLNADGARIIQRGEGVHIFDNHGRKYLDGFAGLWCVNIGYGRREITNAVARQMNE
LPYYNTFFGTSSAPATLLAQKVTSHAGERFNHIFFTGSGSEANDTWFRMARVYWSAVGKPSKKIVIARKNGYHGSTVAGA
SLCGMKYMHEQGDLPIPGIVHIGQPYWYGEGGDLSPDEFGLKVARELETKIDELGEENVAAFVAEPVQGAAGVIIPPETY
WPEIDRICKARDILLVTDEVICGFGRLGAWFGHQHFGVEPDLAPIAKGLSSGYLPIGGVLVSDRIADVLINEVGEFNHGF
TYSGHPVCAAAALENLRIIEEEKLVERVRDDIGPYFGKAWAALADHDLVGEAESVGLMGGLQLAADKSTRRRFDKPDQIG
ALVRNHALANGLVLRATGDRMLASPPLVISHAEVDEMARITRLALDLAWKELKS

Specific function: Involved In Both The Arginine And Lysine Biosynthetic Pathways. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=433, Percent_Identity=27.7136258660508, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4557809, Length=435, Percent_Identity=28.735632183908, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI37574042, Length=367, Percent_Identity=27.5204359673025, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI226442705, Length=364, Percent_Identity=27.7472527472527, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI284507298, Length=299, Percent_Identity=30.7692307692308, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI226442709, Length=335, Percent_Identity=27.1641791044776, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI24119277, Length=369, Percent_Identity=25.4742547425474, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1789759, Length=402, Percent_Identity=28.6069651741293, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693181, Length=415, Percent_Identity=26.0240963855422, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1786991, Length=440, Percent_Identity=27.2727272727273, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788044, Length=399, Percent_Identity=27.8195488721804, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789016, Length=425, Percent_Identity=27.7647058823529, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=28.978622327791, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786349, Length=321, Percent_Identity=23.98753894081, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32564660, Length=444, Percent_Identity=30.6306306306306, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25144271, Length=416, Percent_Identity=27.8846153846154, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71992977, Length=445, Percent_Identity=27.1910112359551, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI25144274, Length=230, Percent_Identity=33.4782608695652, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323470, Length=446, Percent_Identity=26.9058295964126, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324432, Length=327, Percent_Identity=26.9113149847095, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324386, Length=501, Percent_Identity=23.9520958083832, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28574759, Length=439, Percent_Identity=27.3348519362187, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI161085790, Length=387, Percent_Identity=28.6821705426357, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21356575, Length=435, Percent_Identity=28.735632183908, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21357415, Length=438, Percent_Identity=27.3972602739726, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI281366494, Length=373, Percent_Identity=24.3967828418231, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24667139, Length=373, Percent_Identity=24.3967828418231, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24667143, Length=373, Percent_Identity=24.3967828418231, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11 [C]

Molecular weight: Translated: 49654; Mature: 49523

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTYPPSNLAAIDAAHHLHPFADMKKLNADGARIIQRGEGVHIFDNHGRKYLDGFAGLW
CCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCEEEECCCCHHHHHHHCCEE
CVNIGYGRREITNAVARQMNELPYYNTFFGTSSAPATLLAQKVTSHAGERFNHIFFTGSG
EEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
SEANDTWFRMARVYWSAVGKPSKKIVIARKNGYHGSTVAGASLCGMKYMHEQGDLPIPGI
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VHIGQPYWYGEGGDLSPDEFGLKVARELETKIDELGEENVAAFVAEPVQGAAGVIIPPET
EECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCC
YWPEIDRICKARDILLVTDEVICGFGRLGAWFGHQHFGVEPDLAPIAKGLSSGYLPIGGV
CCCHHHHHHHHCCEEEEEHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCE
LVSDRIADVLINEVGEFNHGFTYSGHPVCAAAALENLRIIEEEKLVERVRDDIGPYFGKA
EHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
WAALADHDLVGEAESVGLMGGLQLAADKSTRRRFDKPDQIGALVRNHALANGLVLRATGD
HHHHHCCCCCCCHHHCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCC
RMLASPPLVISHAEVDEMARITRLALDLAWKELKS
CEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTYPPSNLAAIDAAHHLHPFADMKKLNADGARIIQRGEGVHIFDNHGRKYLDGFAGLW
CCCCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCEEEECCCCHHHHHHHCCEE
CVNIGYGRREITNAVARQMNELPYYNTFFGTSSAPATLLAQKVTSHAGERFNHIFFTGSG
EEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
SEANDTWFRMARVYWSAVGKPSKKIVIARKNGYHGSTVAGASLCGMKYMHEQGDLPIPGI
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VHIGQPYWYGEGGDLSPDEFGLKVARELETKIDELGEENVAAFVAEPVQGAAGVIIPPET
EECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCC
YWPEIDRICKARDILLVTDEVICGFGRLGAWFGHQHFGVEPDLAPIAKGLSSGYLPIGGV
CCCHHHHHHHHCCEEEEEHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCE
LVSDRIADVLINEVGEFNHGFTYSGHPVCAAAALENLRIIEEEKLVERVRDDIGPYFGKA
EHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
WAALADHDLVGEAESVGLMGGLQLAADKSTRRRFDKPDQIGALVRNHALANGLVLRATGD
HHHHHCCCCCCCHHHCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCC
RMLASPPLVISHAEVDEMARITRLALDLAWKELKS
CEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-Acetyl-L-ornithine; 2-Oxoglutarate [C]

Specific reaction: N2-Acetyl-L-ornithine + 2-Oxoglutarate <==> N-Acetyl-L-glutamate 5-semialdehyde + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]