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The map label for this gene is thcR [H]

Identifier: 209548531

GI number: 209548531

Start: 945088

End: 946056

Strand: Direct

Name: thcR [H]

Synonym: Rleg2_0928

Alternate gene names: 209548531

Gene position: 945088-946056 (Clockwise)

Preceding gene: 209548530

Following gene: 209548535

Centisome position: 20.83

GC content: 58.62

Gene sequence:

>969_bases
ATGAAACCCGTTACCTTCTCCATGCCGGCGATCGCGATGACAACAAGCGATGTCTCCCAATGTCTCTCAGCTGAAAAGAC
GGTCAATTCCTTTCGCCCGATCAGCAAGGATTTGGTGGGTGACTTTTCCTTCAGCGCGCAGGCGCGGGGTGAATTCGTCG
GATGGGCAGGCTCAAGCTCTGTTCATCGATCGCTGCAGGTCGGCCTGGAAATCGATGACTATCTCTTCTTTACCGACCAT
GGGAACGGCCATTCCATCGCGACCGGGAGTATCAGTTTCGATGTCGGTCATTCCCGTGGCCTGCTGACGTCGGCCGACCG
GTATTCAGGTCTGGAAATCGGGGCAGGCTCGATCGCCGAAGGCTTTTCGGTTCCGAAAGACCTGGTGCACAGGGCGGTTG
CCGATTCTCTGGAATGTTTCGTGCCGAGCGGATTCGAATTTTGTCCGTCTTTTGACCTGGCGACGGGACCGGCCGTGCAA
TTGATGAACCTGATGCGGTTCTTCCGGACGGAAATCTGCGGGCAGCTCGTCGTCTCTCCGATCGCGCTGGCCGGCTTTCA
GGAGATGTTCTGCTCGCTGATGGCCCAGAACATGCAGCATTCGCTGTCGCAAAAGCTTGTGTCGGCTCGGGTGAACTCCA
TCACCCCCGGTCAGTTGAGACGAGCGATGGAATTTGCAAGGGCAAACGCGGCGCTGCCGATCACCATCGCCGACATGGCC
TCAGTGGCCGGTGTCAGCGTACGCACACTGCAAGCCAATTTTCGCAGCTTTCTGAACACGACGCCGACGTCTTTCCTCCG
CCAGCTCCGCTTCGAAGGCGCACGCCGCGACTTAATTCACGCCGCGCCGACAGCGACCGTGACCCACATTGCGCGGCAGT
GGGGATTTGTCCACATGGGACGGTTTTCCGCCGAATACCGGTCGCATTTCGGTGTCTCGCCGTCTGCCGATTTAGGCCGA
CGCAGCTAG

Upstream 100 bases:

>100_bases
GTGAAGGTCTGATGGAGACCGACTATCGGTACTCACAAGTTCCCATTAAGATCCGGAGGCATTCAGCGACCCAAAAGGGG
TCCATATCCAACGAGGCAGA

Downstream 100 bases:

>100_bases
CAACGTCTAAACATCGTCCGGAATGCGCAGCGAGATCACCGTGCCCACGGCTTCGCGGGAGCGGATCTTCATGCGGCCGC
CATGCAGCGCGGTCAGCGAG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKPVTFSMPAIAMTTSDVSQCLSAEKTVNSFRPISKDLVGDFSFSAQARGEFVGWAGSSSVHRSLQVGLEIDDYLFFTDH
GNGHSIATGSISFDVGHSRGLLTSADRYSGLEIGAGSIAEGFSVPKDLVHRAVADSLECFVPSGFEFCPSFDLATGPAVQ
LMNLMRFFRTEICGQLVVSPIALAGFQEMFCSLMAQNMQHSLSQKLVSARVNSITPGQLRRAMEFARANAALPITIADMA
SVAGVSVRTLQANFRSFLNTTPTSFLRQLRFEGARRDLIHAAPTATVTHIARQWGFVHMGRFSAEYRSHFGVSPSADLGR
RS

Sequences:

>Translated_322_residues
MKPVTFSMPAIAMTTSDVSQCLSAEKTVNSFRPISKDLVGDFSFSAQARGEFVGWAGSSSVHRSLQVGLEIDDYLFFTDH
GNGHSIATGSISFDVGHSRGLLTSADRYSGLEIGAGSIAEGFSVPKDLVHRAVADSLECFVPSGFEFCPSFDLATGPAVQ
LMNLMRFFRTEICGQLVVSPIALAGFQEMFCSLMAQNMQHSLSQKLVSARVNSITPGQLRRAMEFARANAALPITIADMA
SVAGVSVRTLQANFRSFLNTTPTSFLRQLRFEGARRDLIHAAPTATVTHIARQWGFVHMGRFSAEYRSHFGVSPSADLGR
RS
>Mature_322_residues
MKPVTFSMPAIAMTTSDVSQCLSAEKTVNSFRPISKDLVGDFSFSAQARGEFVGWAGSSSVHRSLQVGLEIDDYLFFTDH
GNGHSIATGSISFDVGHSRGLLTSADRYSGLEIGAGSIAEGFSVPKDLVHRAVADSLECFVPSGFEFCPSFDLATGPAVQ
LMNLMRFFRTEICGQLVVSPIALAGFQEMFCSLMAQNMQHSLSQKLVSARVNSITPGQLRRAMEFARANAALPITIADMA
SVAGVSVRTLQANFRSFLNTTPTSFLRQLRFEGARRDLIHAAPTATVTHIARQWGFVHMGRFSAEYRSHFGVSPSADLGR
RS

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=104, Percent_Identity=37.5, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 34903; Mature: 34903

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVTFSMPAIAMTTSDVSQCLSAEKTVNSFRPISKDLVGDFSFSAQARGEFVGWAGSSS
CCCCEECCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEECCCCHH
VHRSLQVGLEIDDYLFFTDHGNGHSIATGSISFDVGHSRGLLTSADRYSGLEIGAGSIAE
HHHHHHCCCEEEEEEEEEECCCCCEEEECEEEEECCCCCCCEECCCCCCCCEECCCCHHH
GFSVPKDLVHRAVADSLECFVPSGFEFCPSFDLATGPAVQLMNLMRFFRTEICGQLVVSP
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IALAGFQEMFCSLMAQNMQHSLSQKLVSARVNSITPGQLRRAMEFARANAALPITIADMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
SVAGVSVRTLQANFRSFLNTTPTSFLRQLRFEGARRDLIHAAPTATVTHIARQWGFVHMG
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHH
RFSAEYRSHFGVSPSADLGRRS
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKPVTFSMPAIAMTTSDVSQCLSAEKTVNSFRPISKDLVGDFSFSAQARGEFVGWAGSSS
CCCCEECCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEECCCCHH
VHRSLQVGLEIDDYLFFTDHGNGHSIATGSISFDVGHSRGLLTSADRYSGLEIGAGSIAE
HHHHHHCCCEEEEEEEEEECCCCCEEEECEEEEECCCCCCCEECCCCCCCCEECCCCHHH
GFSVPKDLVHRAVADSLECFVPSGFEFCPSFDLATGPAVQLMNLMRFFRTEICGQLVVSP
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IALAGFQEMFCSLMAQNMQHSLSQKLVSARVNSITPGQLRRAMEFARANAALPITIADMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
SVAGVSVRTLQANFRSFLNTTPTSFLRQLRFEGARRDLIHAAPTATVTHIARQWGFVHMG
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHH
RFSAEYRSHFGVSPSADLGRRS
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]