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The map label for this gene is cmcT [H]

Identifier: 209548504

GI number: 209548504

Start: 916986

End: 918386

Strand: Reverse

Name: cmcT [H]

Synonym: Rleg2_0901

Alternate gene names: 209548504

Gene position: 918386-916986 (Counterclockwise)

Preceding gene: 209548506

Following gene: 209548503

Centisome position: 20.24

GC content: 67.52

Gene sequence:

>1401_bases
ATGACACTTTCCAAATCGAAAGGCGCTGCGGGGCTGGCATTGCTGCTGCTCTGCGCCGCCAACTTTCTCGACGCCATGGA
CGTCTCCACCATCGGTGTCGCGCTTCCCGCCATCCAGGCCGAACTCGGCATGGAGGCGACGTCGCTGCAATGGGCCGTCA
GCGCTTATGTGCTCGGCTATGGCGGCTTTCTGCTGCTCGGCGGCCGGGTGGCCGACGTCTTCGGCCATCGCCGCGTTTTC
CTCTGGTCGCTGGCGATTTTTGCCGCCGCCAGCATCGCCGGCGGCTTCGTCGACAGCGGCCCGACGCTGATCGCCGCCCG
GCTGATCAAGGGCATTGCCGCCGCCTTCACCGCGCCTGCCGCGCTGGCGCTGCTGCTCTCCGTCTTCGGCGAGGGATCGG
CGCGGGCCAAAGCGCTCGGGGTCTTCGCCTCCACCGGCGCCACCGGCTTCGTGCTCGGCATGGTGCTTGGCGGTGCCGCG
ACGATCTTCAGCTGGCGGGCAACGCTGGTCATGGGTGCGCCGGTCGCCATCCTGACGCTCTTTCTTGCGCCGCTGGCGCT
GCCGGCCGACCCGAAAAGGACAGGACCGCGGCCCGCTTTCGACTGGGCCGGCGCGCTGACAATCACCCCCGGGCTGCTGC
TCTTCGTCTTCGGCATCACCAATGCGGCGGCCGCCGGCTGGCAGGCTTTTGCGACCTGGGGCTCGCTCGCGGCATCGCTG
GCGCTGATCCTGCTCTTCCTCGCGGTCGAAGCGCGGCACACCGATCCGATGGTGCCGCTCGGCATGTTCCGCCGGGCGAA
ACTCAGGCACGCCAATGCAATTGCCGCCCTCTTCCAGGGCGCTTATGTCGGCTTCCAGTTCCTGGCGACGCTCTATTATC
AGAACGTCCTCGGCTGGTCGGCCTTCACCACCGGCTTCTGCTTTGCGCTCGGCGGTGTCTTCGTCATGTTCCTGGCGCCC
CGTTTTGCAACGCTGGCACAAAACCGCGGCGCCACCGGGCTGGTGGCGGCAGGTGTCGCCCTGCAGGCCTTCAGCTACAT
CTTCTGGGTAAGCGCGCTCGGACATGTCGACCCGATCCTACTCGTGCTGTTCTCGCAGATCCCGCTCGGCCTCGGTTACG
CACTGACCTACCCTTCCGTGCAGGTCGCGGCCCTTTCCGACGTCGAGGATGACAAATCGGGGCTCGCTTCCGGACTGCTC
TTCGCCTCCTTCCAGATCGGCGGCGGCATCGTGCTCGCAGCCGCCTCGGCAGTGTTCGGCGCGGCACCGCATTTCGGCTG
GGACCCTTATGTCGCCGGCATCGCCTTCGTGGCGCTGCTTGCGGTCGCGATCACCCTGCTTGCCGCTGCCGGTCCGCGGA
CATCGGCCGCCCGGACGCCGGCCTATCAGGCCGCGGAATGA

Upstream 100 bases:

>100_bases
TACTATCAATTTGCAATAGGTCAAGAAGCTGTACATTTTTTCGTTCCCTGCCGTCCGCGCAACCTCCCTCGACGAGGCGG
CAGCAAAAAGGAACGTTTCG

Downstream 100 bases:

>100_bases
CCGTTGCGCCCGCACCCTGACCGGTGCGGGCGCTTCATAAGATAAAACGAATATTAGCGACAGAATCCCATTGCCTTGCC
CCGGCGAAAGGAGCATGGTC

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTLSKSKGAAGLALLLLCAANFLDAMDVSTIGVALPAIQAELGMEATSLQWAVSAYVLGYGGFLLLGGRVADVFGHRRVF
LWSLAIFAAASIAGGFVDSGPTLIAARLIKGIAAAFTAPAALALLLSVFGEGSARAKALGVFASTGATGFVLGMVLGGAA
TIFSWRATLVMGAPVAILTLFLAPLALPADPKRTGPRPAFDWAGALTITPGLLLFVFGITNAAAAGWQAFATWGSLAASL
ALILLFLAVEARHTDPMVPLGMFRRAKLRHANAIAALFQGAYVGFQFLATLYYQNVLGWSAFTTGFCFALGGVFVMFLAP
RFATLAQNRGATGLVAAGVALQAFSYIFWVSALGHVDPILLVLFSQIPLGLGYALTYPSVQVAALSDVEDDKSGLASGLL
FASFQIGGGIVLAAASAVFGAAPHFGWDPYVAGIAFVALLAVAITLLAAAGPRTSAARTPAYQAAE

Sequences:

>Translated_466_residues
MTLSKSKGAAGLALLLLCAANFLDAMDVSTIGVALPAIQAELGMEATSLQWAVSAYVLGYGGFLLLGGRVADVFGHRRVF
LWSLAIFAAASIAGGFVDSGPTLIAARLIKGIAAAFTAPAALALLLSVFGEGSARAKALGVFASTGATGFVLGMVLGGAA
TIFSWRATLVMGAPVAILTLFLAPLALPADPKRTGPRPAFDWAGALTITPGLLLFVFGITNAAAAGWQAFATWGSLAASL
ALILLFLAVEARHTDPMVPLGMFRRAKLRHANAIAALFQGAYVGFQFLATLYYQNVLGWSAFTTGFCFALGGVFVMFLAP
RFATLAQNRGATGLVAAGVALQAFSYIFWVSALGHVDPILLVLFSQIPLGLGYALTYPSVQVAALSDVEDDKSGLASGLL
FASFQIGGGIVLAAASAVFGAAPHFGWDPYVAGIAFVALLAVAITLLAAAGPRTSAARTPAYQAAE
>Mature_465_residues
TLSKSKGAAGLALLLLCAANFLDAMDVSTIGVALPAIQAELGMEATSLQWAVSAYVLGYGGFLLLGGRVADVFGHRRVFL
WSLAIFAAASIAGGFVDSGPTLIAARLIKGIAAAFTAPAALALLLSVFGEGSARAKALGVFASTGATGFVLGMVLGGAAT
IFSWRATLVMGAPVAILTLFLAPLALPADPKRTGPRPAFDWAGALTITPGLLLFVFGITNAAAAGWQAFATWGSLAASLA
LILLFLAVEARHTDPMVPLGMFRRAKLRHANAIAALFQGAYVGFQFLATLYYQNVLGWSAFTTGFCFALGGVFVMFLAPR
FATLAQNRGATGLVAAGVALQAFSYIFWVSALGHVDPILLVLFSQIPLGLGYALTYPSVQVAALSDVEDDKSGLASGLLF
ASFQIGGGIVLAAASAVFGAAPHFGWDPYVAGIAFVALLAVAITLLAAAGPRTSAARTPAYQAAE

Specific function: Involved in cephamycin export [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=437, Percent_Identity=26.7734553775744, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081983, Length=387, Percent_Identity=24.5478036175711, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788710, Length=399, Percent_Identity=23.5588972431078, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789042, Length=353, Percent_Identity=23.7960339943343, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=256, Percent_Identity=27.734375, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323520, Length=293, Percent_Identity=24.5733788395904, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47787; Mature: 47656

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSKSKGAAGLALLLLCAANFLDAMDVSTIGVALPAIQAELGMEATSLQWAVSAYVLGY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GGFLLLGGRVADVFGHRRVFLWSLAIFAAASIAGGFVDSGPTLIAARLIKGIAAAFTAPA
CCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALALLLSVFGEGSARAKALGVFASTGATGFVLGMVLGGAATIFSWRATLVMGAPVAILTL
HHHHHHHHHCCCCCHHHHHEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FLAPLALPADPKRTGPRPAFDWAGALTITPGLLLFVFGITNAAAAGWQAFATWGSLAASL
HHHHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALILLFLAVEARHTDPMVPLGMFRRAKLRHANAIAALFQGAYVGFQFLATLYYQNVLGWS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTTGFCFALGGVFVMFLAPRFATLAQNRGATGLVAAGVALQAFSYIFWVSALGHVDPIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LVLFSQIPLGLGYALTYPSVQVAALSDVEDDKSGLASGLLFASFQIGGGIVLAAASAVFG
HHHHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
AAPHFGWDPYVAGIAFVALLAVAITLLAAAGPRTSAARTPAYQAAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLSKSKGAAGLALLLLCAANFLDAMDVSTIGVALPAIQAELGMEATSLQWAVSAYVLGY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GGFLLLGGRVADVFGHRRVFLWSLAIFAAASIAGGFVDSGPTLIAARLIKGIAAAFTAPA
CCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALALLLSVFGEGSARAKALGVFASTGATGFVLGMVLGGAATIFSWRATLVMGAPVAILTL
HHHHHHHHHCCCCCHHHHHEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FLAPLALPADPKRTGPRPAFDWAGALTITPGLLLFVFGITNAAAAGWQAFATWGSLAASL
HHHHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALILLFLAVEARHTDPMVPLGMFRRAKLRHANAIAALFQGAYVGFQFLATLYYQNVLGWS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTTGFCFALGGVFVMFLAPRFATLAQNRGATGLVAAGVALQAFSYIFWVSALGHVDPIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LVLFSQIPLGLGYALTYPSVQVAALSDVEDDKSGLASGLLFASFQIGGGIVLAAASAVFG
HHHHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
AAPHFGWDPYVAGIAFVALLAVAITLLAAAGPRTSAARTPAYQAAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8440253 [H]