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The map label for this gene is mrpD [H]

Identifier: 209548480

GI number: 209548480

Start: 895554

End: 897107

Strand: Direct

Name: mrpD [H]

Synonym: Rleg2_0877

Alternate gene names: 209548480

Gene position: 895554-897107 (Clockwise)

Preceding gene: 209548479

Following gene: 209548481

Centisome position: 19.73

GC content: 65.32

Gene sequence:

>1554_bases
ATGGCCGCTCCCACCTCCGCCGTCGATCTCTCCGCCGCCCTGGTCACCGCCCCGGTGCCGCTCGGCCATTGGCTTGCCAT
CCTGCCGGTCGTCCATTGCATCACGCTCGGCGCCGTCCTGCTGATGCTGCGCGCCTACCCCCGCCTGCAGGCCTTGCTGG
CCATATCGGGCCTTGCTTTGCTGGCGCTGATCGATGCGGCGCTGCTGGCCAAGGTCGTGGCCCAAGGGCCGCTCACCATG
GTCATGGGCCGCTGGCTGCCGCCCTTCGGCATCGCCTTCACCATCGACCTCTTCGGGGCGCTGATGGCCTTCACCTCGGC
CCTTGCGGCGCTCGCCGCCGGCATCTATGCGCTGACCGATATCGGTGACAGCGGCCGGCGATACGGGTTTTTTCCGCTGC
TGATGCTGCTGATGGCCGGTGTCAGCGGTGCGTTTCTCACCGGCGACGTCTTCAATCTCTATGTCTGGTTCGAGGTGCTG
CTGATCTCCTCCTTCGGGCTGATCATTCTCGGCTCCGAACGTCAGCAGATTGATGGCGCGCTGAAATATGCGGTGCTGAA
CCTGATCGCCACGACGCTGTTCCTGGTTGGCGTCGGCATCCTCTATGCCGTCTTCGGCACCCTCAACATGGCCGATATCG
CGGCAAGGGCGAGGGATTTGCGCACCACGGCGCCGCTGATGACGCTGGCAAGCCTCTTCCTGCTCGCCTTCGCAATGAAG
GCGGCTGCTTTCCCGGTCAATTTCTGGCTGCCCGCCGCCTATCATACGCCGCGCATTGTGGTCTCGGCGCTGTTTGCCGG
CCTGCTCACCAAGGTCGGCATTTACGCATTGATCCGGGTGGTGGTCATGCTGCTGCCGGTGGAGCGCCAGGAATTGAGCC
TGCTGATTGCGTTTATCGCCGCCGCGACCATCCTCGTCGGCGCGCTCGGCGCGCTTGCCGAAAACGATATCCGCAGGCTG
TTCGGCTATGTCGTCATATCGGGCATCGGCAATATGCTCGCCGGCGTCGCACTTGGCGGCACGGGCGGCGGCATCAGCGG
CGCAGTCTTTTACGCGCTCCATTCCATGGTGCTGATGACGGCGCTTTATCTTGCCGCTGGTGAAATCGCCCGGCGCGGCG
GCGGCTTTTTATTGTCGGGGCTCGGCGGCCTCTATCGCCAAAGCGGCGGCTTTACCGCGCTCTCCCTCGTGCTCTTCCTT
GCCGCCTGCGGCCTGCCGCCCTTTTCCGGCTTCTGGCCGAAGGTCATTCTCGTCAAGGCCGCGCTCGATAGCGGCGCCTG
GTGGCTGGCGGCTGTCATCCTCGCCGGCGGTTTTCTGACGACGATCGCTTTCGGCCGCCTCTTTCTGCTCGCCTATTGGC
GGCCGGCGCCGATGGCCGCGAAACCGGCGGCAGCTCTCTGGCGCGCCGGCCTGCCGCTTGCCGCGCTGACGGCGCTGGTC
GTCGGCTTCGGCATCCTGCCGGAACAGCTGCTGGCGCTGTCGCAATCGGCCGCTGCGGGTCTTGCCGATCCGGAAGCCTA
TGTTCATTCGGTCTTTCCCGGAGGCATCAGGTGA

Upstream 100 bases:

>100_bases
CGCGCGTCTTGCCGATCTCCCCCCTTGTGGGGGAGATGCCCGGCAGGGCAGAGGGGGGTATCCCACGGCACGCCCCTCGC
GTCCCCAAAGGCCACGCCTG

Downstream 100 bases:

>100_bases
TCGCCTTTGTCTTCAATCTGCTGCTCGCCATTGCCTGGGTCGCCGTCACCGGCAGCGCCTCGCTGCACAATCTCGTTCTC
GGTTTCCTGCTTGCCGCCCT

Product: monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MAAPTSAVDLSAALVTAPVPLGHWLAILPVVHCITLGAVLLMLRAYPRLQALLAISGLALLALIDAALLAKVVAQGPLTM
VMGRWLPPFGIAFTIDLFGALMAFTSALAALAAGIYALTDIGDSGRRYGFFPLLMLLMAGVSGAFLTGDVFNLYVWFEVL
LISSFGLIILGSERQQIDGALKYAVLNLIATTLFLVGVGILYAVFGTLNMADIAARARDLRTTAPLMTLASLFLLAFAMK
AAAFPVNFWLPAAYHTPRIVVSALFAGLLTKVGIYALIRVVVMLLPVERQELSLLIAFIAAATILVGALGALAENDIRRL
FGYVVISGIGNMLAGVALGGTGGGISGAVFYALHSMVLMTALYLAAGEIARRGGGFLLSGLGGLYRQSGGFTALSLVLFL
AACGLPPFSGFWPKVILVKAALDSGAWWLAAVILAGGFLTTIAFGRLFLLAYWRPAPMAAKPAAALWRAGLPLAALTALV
VGFGILPEQLLALSQSAAAGLADPEAYVHSVFPGGIR

Sequences:

>Translated_517_residues
MAAPTSAVDLSAALVTAPVPLGHWLAILPVVHCITLGAVLLMLRAYPRLQALLAISGLALLALIDAALLAKVVAQGPLTM
VMGRWLPPFGIAFTIDLFGALMAFTSALAALAAGIYALTDIGDSGRRYGFFPLLMLLMAGVSGAFLTGDVFNLYVWFEVL
LISSFGLIILGSERQQIDGALKYAVLNLIATTLFLVGVGILYAVFGTLNMADIAARARDLRTTAPLMTLASLFLLAFAMK
AAAFPVNFWLPAAYHTPRIVVSALFAGLLTKVGIYALIRVVVMLLPVERQELSLLIAFIAAATILVGALGALAENDIRRL
FGYVVISGIGNMLAGVALGGTGGGISGAVFYALHSMVLMTALYLAAGEIARRGGGFLLSGLGGLYRQSGGFTALSLVLFL
AACGLPPFSGFWPKVILVKAALDSGAWWLAAVILAGGFLTTIAFGRLFLLAYWRPAPMAAKPAAALWRAGLPLAALTALV
VGFGILPEQLLALSQSAAAGLADPEAYVHSVFPGGIR
>Mature_516_residues
AAPTSAVDLSAALVTAPVPLGHWLAILPVVHCITLGAVLLMLRAYPRLQALLAISGLALLALIDAALLAKVVAQGPLTMV
MGRWLPPFGIAFTIDLFGALMAFTSALAALAAGIYALTDIGDSGRRYGFFPLLMLLMAGVSGAFLTGDVFNLYVWFEVLL
ISSFGLIILGSERQQIDGALKYAVLNLIATTLFLVGVGILYAVFGTLNMADIAARARDLRTTAPLMTLASLFLLAFAMKA
AAFPVNFWLPAAYHTPRIVVSALFAGLLTKVGIYALIRVVVMLLPVERQELSLLIAFIAAATILVGALGALAENDIRRLF
GYVVISGIGNMLAGVALGGTGGGISGAVFYALHSMVLMTALYLAAGEIARRGGGFLLSGLGGLYRQSGGFTALSLVLFLA
ACGLPPFSGFWPKVILVKAALDSGAWWLAAVILAGGFLTTIAFGRLFLLAYWRPAPMAAKPAAALWRAGLPLAALTALVV
GFGILPEQLLALSQSAAAGLADPEAYVHSVFPGGIR

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=354, Percent_Identity=28.5310734463277, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788613, Length=335, Percent_Identity=27.1641791044776, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693160, Length=423, Percent_Identity=23.1678486997636, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788829, Length=375, Percent_Identity=25.3333333333333, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788831, Length=311, Percent_Identity=28.2958199356913, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788614, Length=449, Percent_Identity=23.8307349665924, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53873; Mature: 53742

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPTSAVDLSAALVTAPVPLGHWLAILPVVHCITLGAVLLMLRAYPRLQALLAISGLAL
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALIDAALLAKVVAQGPLTMVMGRWLPPFGIAFTIDLFGALMAFTSALAALAAGIYALTD
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IGDSGRRYGFFPLLMLLMAGVSGAFLTGDVFNLYVWFEVLLISSFGLIILGSERQQIDGA
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
LKYAVLNLIATTLFLVGVGILYAVFGTLNMADIAARARDLRTTAPLMTLASLFLLAFAMK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAFPVNFWLPAAYHTPRIVVSALFAGLLTKVGIYALIRVVVMLLPVERQELSLLIAFIA
HHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AATILVGALGALAENDIRRLFGYVVISGIGNMLAGVALGGTGGGISGAVFYALHSMVLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ALYLAAGEIARRGGGFLLSGLGGLYRQSGGFTALSLVLFLAACGLPPFSGFWPKVILVKA
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ALDSGAWWLAAVILAGGFLTTIAFGRLFLLAYWRPAPMAAKPAAALWRAGLPLAALTALV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHH
VGFGILPEQLLALSQSAAAGLADPEAYVHSVFPGGIR
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
AAPTSAVDLSAALVTAPVPLGHWLAILPVVHCITLGAVLLMLRAYPRLQALLAISGLAL
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALIDAALLAKVVAQGPLTMVMGRWLPPFGIAFTIDLFGALMAFTSALAALAAGIYALTD
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IGDSGRRYGFFPLLMLLMAGVSGAFLTGDVFNLYVWFEVLLISSFGLIILGSERQQIDGA
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
LKYAVLNLIATTLFLVGVGILYAVFGTLNMADIAARARDLRTTAPLMTLASLFLLAFAMK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAFPVNFWLPAAYHTPRIVVSALFAGLLTKVGIYALIRVVVMLLPVERQELSLLIAFIA
HHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AATILVGALGALAENDIRRLFGYVVISGIGNMLAGVALGGTGGGISGAVFYALHSMVLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ALYLAAGEIARRGGGFLLSGLGGLYRQSGGFTALSLVLFLAACGLPPFSGFWPKVILVKA
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ALDSGAWWLAAVILAGGFLTTIAFGRLFLLAYWRPAPMAAKPAAALWRAGLPLAALTALV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHH
VGFGILPEQLLALSQSAAAGLADPEAYVHSVFPGGIR
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]