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The map label for this gene is cspA [H]

Identifier: 209548412

GI number: 209548412

Start: 828591

End: 828800

Strand: Direct

Name: cspA [H]

Synonym: Rleg2_0809

Alternate gene names: 209548412

Gene position: 828591-828800 (Clockwise)

Preceding gene: 209548411

Following gene: 209548413

Centisome position: 18.26

GC content: 53.81

Gene sequence:

>210_bases
ATGAATTCAGGCGTCGTCAAGTGGTTCAATGGCACCAAGGGTTTTGGCTTCATTCAGCCCGATGATGGTTCTACGGATGT
TTTTGTCCACATTTCAGCGGTCGAACGCGCCGGCATGCGCGAACTCGTTGAAGGCCAGAAGATCCGCTACGATCTCGTCC
GCGACAAGAAGTCGGGCAAGAATTCGGCTGACAACCTTCAGGCCGCATGA

Upstream 100 bases:

>100_bases
TCCACGCGCTGAAGCGCTCCGTCAGATATCCTCTCAACATCGATACGGCTTGTCGGACATTCTTCCGCAGGCGAACACAA
CGGTTGAAAAGGAGATCGTT

Downstream 100 bases:

>100_bases
TTTGTCATTCGCCTATGCTGTCCAGATGCTGGCAGCGCGCTGACCACGGATGTACTGGCAGCGAGCTGACCGACTGGCGG
AGAAATGGCTGAGTGACTGG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 69

Protein sequence:

>69_residues
MNSGVVKWFNGTKGFGFIQPDDGSTDVFVHISAVERAGMRELVEGQKIRYDLVRDKKSGKNSADNLQAA

Sequences:

>Translated_69_residues
MNSGVVKWFNGTKGFGFIQPDDGSTDVFVHISAVERAGMRELVEGQKIRYDLVRDKKSGKNSADNLQAA
>Mature_69_residues
MNSGVVKWFNGTKGFGFIQPDDGSTDVFVHISAVERAGMRELVEGQKIRYDLVRDKKSGKNSADNLQAA

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=50, Percent_Identity=60, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1786841, Length=54, Percent_Identity=53.7037037037037, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1787839, Length=50, Percent_Identity=58, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI2367114, Length=50, Percent_Identity=56, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1788126, Length=49, Percent_Identity=57.1428571428571, Blast_Score=66, Evalue=3e-13,
Organism=Escherichia coli, GI1787834, Length=50, Percent_Identity=52, Blast_Score=63, Evalue=3e-12,
Organism=Escherichia coli, GI1787107, Length=62, Percent_Identity=43.5483870967742, Blast_Score=62, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7588; Mature: 7588

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSGVVKWFNGTKGFGFIQPDDGSTDVFVHISAVERAGMRELVEGQKIRYDLVRDKKSGK
CCCCEEEECCCCCCCEEECCCCCCCEEEEEEECHHHCCHHHHHCCCCEEEEEECCCCCCC
NSADNLQAA
CCCCCCCCC
>Mature Secondary Structure
MNSGVVKWFNGTKGFGFIQPDDGSTDVFVHISAVERAGMRELVEGQKIRYDLVRDKKSGK
CCCCEEEECCCCCCCEEECCCCCCCEEEEEEECHHHCCHHHHHCCCCEEEEEECCCCCCC
NSADNLQAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10618253; 11481430 [H]