Click here to switch to the map view.

The map label for this gene is pbpF [H]

Identifier: 209548371

GI number: 209548371

Start: 783927

End: 786218

Strand: Reverse

Name: pbpF [H]

Synonym: Rleg2_0767

Alternate gene names: 209548371

Gene position: 786218-783927 (Counterclockwise)

Preceding gene: 209548372

Following gene: 209548370

Centisome position: 17.33

GC content: 62.04

Gene sequence:

>2292_bases
ATGCCCAATTCTCCCGAATCCTCCGGTGAAAGCGTCAGCAGCTCGAGCTCGGATCGCGAGAAGACACTGGATTCGGCAGT
GCAAGCGGACGCCTTGGATTCGCCCGGAACCCAAGGCGAAGACCCGCAGCCGCCCCATGGATCCTTCGAGCAAACCAGGC
GGGCCGCCAGGACGCTATTCGGTGCGATACGCCAGGATTTGCAGGCAAGTTCTGCTTTGGCAAAGCTCAAGGCGGCCGCC
TTGTTATTGAAGACGAAGCTAACAAGGTCGGGGCGGCCTGGAGTCACTTCTCCCCACAAATGGCTGCCGACGGCTTGGGT
CAAATCCTGGCGCGGTATTCGTGATCGGCGTTCAAAGCCGCGTGAAAATCGTCGGAGCGCATCGTTCATTGCGGGCTGGC
GGAAATTCGCTCTCGGGTTCACCCTTCTTGTCTGCCTCCTCGTCGGAAGCGTGCTGGTCTGGGCCCTGAAAGACGTGCCC
TGGAGCGAAATCCGGGATGGAACGTTAAAGCCGGTCGTGGTGCTGGAAACCGCCGACGGCGCGCCGCTGGTCAGACAGGG
ACCTTATCAAGGGCCATATGCTCGATACGACCAGTTTCCGCCCCATCTGATCGATGCCGTCCTTTCCATCGAGGATCGCC
GGTTCATGGATCATTTCGGCATCGATCCCAGGGGCATCGCCAGGGCGTTTTGGCGGAACCTGGAGGCTGGCTCGGTGGTG
GAGGGTGGCAGTACCATCACCCAGCAACTGATCAAGCTGCAATATCTTGACAGCGACCGAACGATAAAACGAAAGATTCA
GGAGGTGGTGATCGCCTTCTGGCTGGAGTGGAAGCTCGGCAAGGCGGAAATCCTGACGCGTTATCTCAACAGCGTCTATC
TTGGCGCGGGCGCCACCGGCATGCCGGCCGCGGCGCGCATCTATTTCAACAAGGATATCGGCGCCCTCAACCTGCCGGAG
TCGGCGATGCTGGCGGGATTGCTGCGGGCGCCGAGCCAATGGAATCCCATCGACAATTTCGAAGGCGCCCGGCAACGCAC
CATGGTCGTTCTCGATGCGATGGCGGCCAATGGCAAGATCACCGCACCACAGGCGGCCGAGGCCAAGACGAGCTTTGCCA
GCCTCCACCCAACAACACCAACGCCGCGTTCCGGAAGCTGGTTTGCCGACTGGATTTCGCCGCAAGCGAGCGAAATCGCC
GGCGCCTCGCCGGGTTCGACCACGGTGCGCACCACGCTGGTGCCGACATTGCAGCAGATCGCCGAAAGGGTGGTGAAAAA
AGGGCTCGACGGTGAAGGAAAGACGGTCGGAGCATCGCAGGCCGCCTTGGTTGCGATGACGCCCGACGGCGCGGTCGTCG
CGATGGTTGGCGGGCGCGACTACAAGGCAAGCCAGTTCAACCGCGCCGTCACCGCAATGCGCCAGCCGGGTTCCACCTTC
AAGCTGTTCGTCTATTACGCAGCATTGAAGGCTGGGCTCACTCTGTCCGACCAGGTTCTGGACGCGCCCATAGACATTGA
CGGCTGGTCGCCCGAAAATTCCGGCGGCGATTATCGCGGTTGGGTCACGCTTGCCGAGGCGTTCGCTCGATCGTTGAATG
CGGCCTCGGTGACGCTTGCGCAAGAGGTCGGCCTCGACAATGTGATTGCCGCCGCGCGCGAACTCGGCATCGATGCGCCA
CTGGCCAACACGCCGTCATTGGCGCTTGGCACATCCGAAGTCAATCTGCTCAACCTCACCAGCGCCTATGCGTCGGTCCA
GCTCGGCAGGGCCCCTGTCAAACCCTGGGGCATCATCGACTTTCAAGCGGCGGGGCAGCCCCAGACATTTCGTGTCGGCT
TACAATCCAAGCCGACTGTCGATCTTTCGCCCTACCAGTCCGATCTGCTCGGCCTCCTGCAGCTGGTGGTCGAGCGCGGC
ACGGGACGCGGAGCAGATCCGGGCACCTTTGCCGCCGGCAAGACCGGCACGAGCCAGAACAATCGCGATGCCTGGTTCGT
CGGCTTCACGGAAGCGCTCGTCGTCGGTGTCTGGGTTGGCAATGATGACGACGCGCCGATGAAGGGAGTGACCGGCGGCG
CCCTGCCGGCCCATATCTGGCGGGACTTCATGCGCGAGGCGATGAGCAAACCGACATTGGACGGGGTGCGATCGACAGAA
GCAGCCGTCGATGGACAAGGCGCGCCGCAATCCTGCAACATCACCGCCTGCTCTCGCAGCTACCGCTCCTTCCGGCCGTC
CGATTGTACCTATCAGCCATATTCCGGCGGCCGACGGCTTTGCGAAAAGTGA

Upstream 100 bases:

>100_bases
AAAACGCGCTCGACACTCACTGCGCGGTTGCTCAGTGGGTTCCTGACCCTCTACGACTGGGCGGTAGATCATCAAAACGA
CCTGTCCGAGGCGGTAACCG

Downstream 100 bases:

>100_bases
TGTGGATGAGCCGAGATTGCGCCGGCCGCCCTGCCTGACGCGATATTGCCGATCCGTGAGGCGCTGCCCACACGCATCGG
CGCCACTTATCCACAAAACG

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MPNSPESSGESVSSSSSDREKTLDSAVQADALDSPGTQGEDPQPPHGSFEQTRRAARTLFGAIRQDLQASSALAKLKAAA
LLLKTKLTRSGRPGVTSPHKWLPTAWVKSWRGIRDRRSKPRENRRSASFIAGWRKFALGFTLLVCLLVGSVLVWALKDVP
WSEIRDGTLKPVVVLETADGAPLVRQGPYQGPYARYDQFPPHLIDAVLSIEDRRFMDHFGIDPRGIARAFWRNLEAGSVV
EGGSTITQQLIKLQYLDSDRTIKRKIQEVVIAFWLEWKLGKAEILTRYLNSVYLGAGATGMPAAARIYFNKDIGALNLPE
SAMLAGLLRAPSQWNPIDNFEGARQRTMVVLDAMAANGKITAPQAAEAKTSFASLHPTTPTPRSGSWFADWISPQASEIA
GASPGSTTVRTTLVPTLQQIAERVVKKGLDGEGKTVGASQAALVAMTPDGAVVAMVGGRDYKASQFNRAVTAMRQPGSTF
KLFVYYAALKAGLTLSDQVLDAPIDIDGWSPENSGGDYRGWVTLAEAFARSLNAASVTLAQEVGLDNVIAAARELGIDAP
LANTPSLALGTSEVNLLNLTSAYASVQLGRAPVKPWGIIDFQAAGQPQTFRVGLQSKPTVDLSPYQSDLLGLLQLVVERG
TGRGADPGTFAAGKTGTSQNNRDAWFVGFTEALVVGVWVGNDDDAPMKGVTGGALPAHIWRDFMREAMSKPTLDGVRSTE
AAVDGQGAPQSCNITACSRSYRSFRPSDCTYQPYSGGRRLCEK

Sequences:

>Translated_763_residues
MPNSPESSGESVSSSSSDREKTLDSAVQADALDSPGTQGEDPQPPHGSFEQTRRAARTLFGAIRQDLQASSALAKLKAAA
LLLKTKLTRSGRPGVTSPHKWLPTAWVKSWRGIRDRRSKPRENRRSASFIAGWRKFALGFTLLVCLLVGSVLVWALKDVP
WSEIRDGTLKPVVVLETADGAPLVRQGPYQGPYARYDQFPPHLIDAVLSIEDRRFMDHFGIDPRGIARAFWRNLEAGSVV
EGGSTITQQLIKLQYLDSDRTIKRKIQEVVIAFWLEWKLGKAEILTRYLNSVYLGAGATGMPAAARIYFNKDIGALNLPE
SAMLAGLLRAPSQWNPIDNFEGARQRTMVVLDAMAANGKITAPQAAEAKTSFASLHPTTPTPRSGSWFADWISPQASEIA
GASPGSTTVRTTLVPTLQQIAERVVKKGLDGEGKTVGASQAALVAMTPDGAVVAMVGGRDYKASQFNRAVTAMRQPGSTF
KLFVYYAALKAGLTLSDQVLDAPIDIDGWSPENSGGDYRGWVTLAEAFARSLNAASVTLAQEVGLDNVIAAARELGIDAP
LANTPSLALGTSEVNLLNLTSAYASVQLGRAPVKPWGIIDFQAAGQPQTFRVGLQSKPTVDLSPYQSDLLGLLQLVVERG
TGRGADPGTFAAGKTGTSQNNRDAWFVGFTEALVVGVWVGNDDDAPMKGVTGGALPAHIWRDFMREAMSKPTLDGVRSTE
AAVDGQGAPQSCNITACSRSYRSFRPSDCTYQPYSGGRRLCEK
>Mature_762_residues
PNSPESSGESVSSSSSDREKTLDSAVQADALDSPGTQGEDPQPPHGSFEQTRRAARTLFGAIRQDLQASSALAKLKAAAL
LLKTKLTRSGRPGVTSPHKWLPTAWVKSWRGIRDRRSKPRENRRSASFIAGWRKFALGFTLLVCLLVGSVLVWALKDVPW
SEIRDGTLKPVVVLETADGAPLVRQGPYQGPYARYDQFPPHLIDAVLSIEDRRFMDHFGIDPRGIARAFWRNLEAGSVVE
GGSTITQQLIKLQYLDSDRTIKRKIQEVVIAFWLEWKLGKAEILTRYLNSVYLGAGATGMPAAARIYFNKDIGALNLPES
AMLAGLLRAPSQWNPIDNFEGARQRTMVVLDAMAANGKITAPQAAEAKTSFASLHPTTPTPRSGSWFADWISPQASEIAG
ASPGSTTVRTTLVPTLQQIAERVVKKGLDGEGKTVGASQAALVAMTPDGAVVAMVGGRDYKASQFNRAVTAMRQPGSTFK
LFVYYAALKAGLTLSDQVLDAPIDIDGWSPENSGGDYRGWVTLAEAFARSLNAASVTLAQEVGLDNVIAAARELGIDAPL
ANTPSLALGTSEVNLLNLTSAYASVQLGRAPVKPWGIIDFQAAGQPQTFRVGLQSKPTVDLSPYQSDLLGLLQLVVERGT
GRGADPGTFAAGKTGTSQNNRDAWFVGFTEALVVGVWVGNDDDAPMKGVTGGALPAHIWRDFMREAMSKPTLDGVRSTEA
AVDGQGAPQSCNITACSRSYRSFRPSDCTYQPYSGGRRLCEK

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=551, Percent_Identity=31.3974591651543, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788867, Length=584, Percent_Identity=30.3082191780822, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI87082258, Length=306, Percent_Identity=34.9673202614379, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1789601, Length=203, Percent_Identity=32.512315270936, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 82035; Mature: 81904

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSPESSGESVSSSSSDREKTLDSAVQADALDSPGTQGEDPQPPHGSFEQTRRAARTLF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GAIRQDLQASSALAKLKAAALLLKTKLTRSGRPGVTSPHKWLPTAWVKSWRGIRDRRSKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCHHHHHCCC
RENRRSASFIAGWRKFALGFTLLVCLLVGSVLVWALKDVPWSEIRDGTLKPVVVLETADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCC
APLVRQGPYQGPYARYDQFPPHLIDAVLSIEDRRFMDHFGIDPRGIARAFWRNLEAGSVV
CCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEE
EGGSTITQQLIKLQYLDSDRTIKRKIQEVVIAFWLEWKLGKAEILTRYLNSVYLGAGATG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCC
MPAAARIYFNKDIGALNLPESAMLAGLLRAPSQWNPIDNFEGARQRTMVVLDAMAANGKI
CCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEEEHHCCCEE
TAPQAAEAKTSFASLHPTTPTPRSGSWFADWISPQASEIAGASPGSTTVRTTLVPTLQQI
CCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCHHHHCCCCCCCCEEHHHHHHHHHHH
AERVVKKGLDGEGKTVGASQAALVAMTPDGAVVAMVGGRDYKASQFNRAVTAMRQPGSTF
HHHHHHCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCE
KLFVYYAALKAGLTLSDQVLDAPIDIDGWSPENSGGDYRGWVTLAEAFARSLNAASVTLA
EHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
QEVGLDNVIAAARELGIDAPLANTPSLALGTSEVNLLNLTSAYASVQLGRAPVKPWGIID
HHHCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEHHHHHHHHCCCCCCCCCCCEEE
FQAAGQPQTFRVGLQSKPTVDLSPYQSDLLGLLQLVVERGTGRGADPGTFAAGKTGTSQN
EECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
NRDAWFVGFTEALVVGVWVGNDDDAPMKGVTGGALPAHIWRDFMREAMSKPTLDGVRSTE
CCCEEEEEEHHEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
AAVDGQGAPQSCNITACSRSYRSFRPSDCTYQPYSGGRRLCEK
HHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
PNSPESSGESVSSSSSDREKTLDSAVQADALDSPGTQGEDPQPPHGSFEQTRRAARTLF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GAIRQDLQASSALAKLKAAALLLKTKLTRSGRPGVTSPHKWLPTAWVKSWRGIRDRRSKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCHHHHHCCC
RENRRSASFIAGWRKFALGFTLLVCLLVGSVLVWALKDVPWSEIRDGTLKPVVVLETADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCC
APLVRQGPYQGPYARYDQFPPHLIDAVLSIEDRRFMDHFGIDPRGIARAFWRNLEAGSVV
CCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEE
EGGSTITQQLIKLQYLDSDRTIKRKIQEVVIAFWLEWKLGKAEILTRYLNSVYLGAGATG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCC
MPAAARIYFNKDIGALNLPESAMLAGLLRAPSQWNPIDNFEGARQRTMVVLDAMAANGKI
CCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEEEHHCCCEE
TAPQAAEAKTSFASLHPTTPTPRSGSWFADWISPQASEIAGASPGSTTVRTTLVPTLQQI
CCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCHHHHCCCCCCCCEEHHHHHHHHHHH
AERVVKKGLDGEGKTVGASQAALVAMTPDGAVVAMVGGRDYKASQFNRAVTAMRQPGSTF
HHHHHHCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCE
KLFVYYAALKAGLTLSDQVLDAPIDIDGWSPENSGGDYRGWVTLAEAFARSLNAASVTLA
EHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
QEVGLDNVIAAARELGIDAPLANTPSLALGTSEVNLLNLTSAYASVQLGRAPVKPWGIID
HHHCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEHHHHHHHHCCCCCCCCCCCEEE
FQAAGQPQTFRVGLQSKPTVDLSPYQSDLLGLLQLVVERGTGRGADPGTFAAGKTGTSQN
EECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
NRDAWFVGFTEALVVGVWVGNDDDAPMKGVTGGALPAHIWRDFMREAMSKPTLDGVRSTE
CCCEEEEEEHHEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
AAVDGQGAPQSCNITACSRSYRSFRPSDCTYQPYSGGRRLCEK
HHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]