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The map label for this gene is yafC [H]

Identifier: 209548369

GI number: 209548369

Start: 781737

End: 782657

Strand: Direct

Name: yafC [H]

Synonym: Rleg2_0765

Alternate gene names: 209548369

Gene position: 781737-782657 (Clockwise)

Preceding gene: 209548368

Following gene: 209548376

Centisome position: 17.23

GC content: 63.95

Gene sequence:

>921_bases
ATGGATAAGGTCGGCCTGTCTTTTGACAGAATGCGCACCTTTGTTCGCGTCGCCGAGCGCGGCAACCTGTCGATGGTCGC
AAGGGAGCTGGATGTCGGCCAATCCACCGTGACGCGGCACCTCAATAAACTTGAGGAGGCTGTCGGGGTGCCGCTTCTCA
GCCGCACCACCCGTCGCGTCACCCTGACCGAGGAAGGCAGCCGCTATTATACCAACTGCCTGCAGATATTGCGCCTGGTC
GAACAGGCTGCTGAAGAAGCGAGAGATGCACGTCAGGCCCCCGCCGGCGGCGTTCGCCTTTCCTGTACGGCAGCGCTTGG
CGTCATGCATGTCACGCGCATGATCTTTGAGTTTCAGGACAGGCATCCGGATATCCGCGTCGACCTCAACCTGACGGACG
AGCGTATCGACCTTGTGCGCGAGGCCGTCGACGTGGCGCTCCGTCTTGGGCCTCTCGCCGACAGCGCAATGAAGCTTCAT
GCGATCGGAGAGAGCCAGCGGCTGCTGGTCGGCGCCCCTGCCTATCTGTCCGCCCGCGGACGGCCGCAGCGCCCGGCTGA
TCTGTCGCGCTACGAAACCGTCGTGATGTCCAACGTGGCAGGCAGCAATCAGCTGCTTCTTTCCGCTCCCGATGGCACCA
GCCTGATGATCCCGGTCAGCGGTCGGTTGCGCGTCGATCACGGGCTTGCGGCGCGCGAAGCTCTTGCCGCCGGACGCGGC
ATTGGTCCCACACATCTCTGGCTGGTTCACGACCTGCTGGACGACGGCCGGCTGGAGATCGTGCTTGGCGACTATCGGCC
TTCGCCGGTTCCGGTGAGCCTGCTGATTGTTCCGGAGCGTGCGGCCATTGCCCGCGTTCGGCTCCTCGTCGACTTTTTGG
CCGCCGAGGTTGCCAAGCTGCCGGGAATCCGGCCGTCGTAA

Upstream 100 bases:

>100_bases
CCGCGACAATAGACGTGGAGCCGAGCCTTGCTGAAGCCTTCACTAGGCATGGTCGGGATTATTCCGTATATGGTCAGAAT
GGCAATCACTGTGCTGAAAA

Downstream 100 bases:

>100_bases
GCCTCTTGTTTTCAGGCATCCCGTTATCGCAAAACCGCTGCCGCACTTTTGCGAGCCATGCTTCAACCTGCGAAAGACCG
CTTGTCGCCGCGCCACCCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MDKVGLSFDRMRTFVRVAERGNLSMVARELDVGQSTVTRHLNKLEEAVGVPLLSRTTRRVTLTEEGSRYYTNCLQILRLV
EQAAEEARDARQAPAGGVRLSCTAALGVMHVTRMIFEFQDRHPDIRVDLNLTDERIDLVREAVDVALRLGPLADSAMKLH
AIGESQRLLVGAPAYLSARGRPQRPADLSRYETVVMSNVAGSNQLLLSAPDGTSLMIPVSGRLRVDHGLAAREALAAGRG
IGPTHLWLVHDLLDDGRLEIVLGDYRPSPVPVSLLIVPERAAIARVRLLVDFLAAEVAKLPGIRPS

Sequences:

>Translated_306_residues
MDKVGLSFDRMRTFVRVAERGNLSMVARELDVGQSTVTRHLNKLEEAVGVPLLSRTTRRVTLTEEGSRYYTNCLQILRLV
EQAAEEARDARQAPAGGVRLSCTAALGVMHVTRMIFEFQDRHPDIRVDLNLTDERIDLVREAVDVALRLGPLADSAMKLH
AIGESQRLLVGAPAYLSARGRPQRPADLSRYETVVMSNVAGSNQLLLSAPDGTSLMIPVSGRLRVDHGLAAREALAAGRG
IGPTHLWLVHDLLDDGRLEIVLGDYRPSPVPVSLLIVPERAAIARVRLLVDFLAAEVAKLPGIRPS
>Mature_306_residues
MDKVGLSFDRMRTFVRVAERGNLSMVARELDVGQSTVTRHLNKLEEAVGVPLLSRTTRRVTLTEEGSRYYTNCLQILRLV
EQAAEEARDARQAPAGGVRLSCTAALGVMHVTRMIFEFQDRHPDIRVDLNLTDERIDLVREAVDVALRLGPLADSAMKLH
AIGESQRLLVGAPAYLSARGRPQRPADLSRYETVVMSNVAGSNQLLLSAPDGTSLMIPVSGRLRVDHGLAAREALAAGRG
IGPTHLWLVHDLLDDGRLEIVLGDYRPSPVPVSLLIVPERAAIARVRLLVDFLAAEVAKLPGIRPS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=29.8305084745763, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=30.0675675675676, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=31.1864406779661, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=27.3684210526316, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=28.9752650176678, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=28.0405405405405, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787589, Length=187, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=224, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=171, Percent_Identity=25.1461988304094, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=212, Percent_Identity=24.5283018867925, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=148, Percent_Identity=27.7027027027027, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789173, Length=185, Percent_Identity=24.8648648648649, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=118, Percent_Identity=27.9661016949153, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33493; Mature: 33493

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVGLSFDRMRTFVRVAERGNLSMVARELDVGQSTVTRHLNKLEEAVGVPLLSRTTRRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCEEE
TLTEEGSRYYTNCLQILRLVEQAAEEARDARQAPAGGVRLSCTAALGVMHVTRMIFEFQD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHC
RHPDIRVDLNLTDERIDLVREAVDVALRLGPLADSAMKLHAIGESQRLLVGAPAYLSARG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHCCCC
RPQRPADLSRYETVVMSNVAGSNQLLLSAPDGTSLMIPVSGRLRVDHGLAAREALAAGRG
CCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCEEECCCHHHHHHHHHCCC
IGPTHLWLVHDLLDDGRLEIVLGDYRPSPVPVSLLIVPERAAIARVRLLVDFLAAEVAKL
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHC
PGIRPS
CCCCCC
>Mature Secondary Structure
MDKVGLSFDRMRTFVRVAERGNLSMVARELDVGQSTVTRHLNKLEEAVGVPLLSRTTRRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCEEE
TLTEEGSRYYTNCLQILRLVEQAAEEARDARQAPAGGVRLSCTAALGVMHVTRMIFEFQD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHC
RHPDIRVDLNLTDERIDLVREAVDVALRLGPLADSAMKLHAIGESQRLLVGAPAYLSARG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHCCCC
RPQRPADLSRYETVVMSNVAGSNQLLLSAPDGTSLMIPVSGRLRVDHGLAAREALAAGRG
CCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCEEECCCHHHHHHHHHCCC
IGPTHLWLVHDLLDDGRLEIVLGDYRPSPVPVSLLIVPERAAIARVRLLVDFLAAEVAKL
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHC
PGIRPS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]