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The map label for this gene is ppdK [H]

Identifier: 209548267

GI number: 209548267

Start: 686940

End: 689624

Strand: Direct

Name: ppdK [H]

Synonym: Rleg2_0662

Alternate gene names: 209548267

Gene position: 686940-689624 (Clockwise)

Preceding gene: 209548262

Following gene: 209548270

Centisome position: 15.14

GC content: 64.8

Gene sequence:

>2685_bases
ATGACCAAGTGGGTCTATACGTTCGGCGACGGGCAGGCTGAGGGCCGGGCGCGCGATCACGAAATTCTGGGCGGCAAGGG
CGCCAATCTGGCGGAGATGTGCGCGCTGGGATTGCCCGTTCCGCCGGGGCTGACGATCATCGGCGATGCCTGCAACACCT
ATTACCGGAACGGCCGCCATATCGAGGACGGCGTAAAGGCCGAGGTGCGCGCCGGCATATCAGGCATCGAGGCGATCACC
GGCCGCCGCTTCGGCGCCGGCAGCCAGCCGCTGCTGCTCTCGGTGCGCTCCGGCGCCCGCGTTTCCATGCCCGGCATGAT
GGACACCGTGCTCAATCTCGGCCTCAACGACGAGACGGTGCAGGCGCTCGGCCACGATGCCGGCGACGCGCGTTTTGCCT
GGGACAGCTACCGCCGCTTCATCCAGATGTATGCCGATGTCGTCATGGGTCTCGGCAACGACGCCTTCGAGGAGATCCTC
GAAGACGAGAAGGCCAAGCTCGGCCATGAAACCGATACCGAACTCAGCGCCTCCGAGTGGCAGCATATCGTCTTCCTCTA
CAAGAAGCTGATCGAGGAGGAGCTGGAGCAGGAATTCCCGCAGGACCCTGAGGTTCAGCTCTGGGGCGCCGTCGGCGCGG
TCTTTTCCAGCTGGATGAGCGCGCGCGCCGTCACCTACCGCCAGCTTCATAATATTCCGGAAGGCTGGGGCACGGCGGTC
AACATCCAGGCGATGGTCTTCGGCAATCTCGGCAATGCCTCGGCGACCGGCGTCGCCTTCACCCGCAACCCCTCGACCGG
CGAGAAGGCGCTTTACGGCGAATTCCTGGTCAATGCGCAAGGCGAGGATGTCGTGGCCGGCATCCGCACGCCGCAAAGCA
TCACCGAGGAGGGCCGTCTTTCCTCCGGCTCGGAAAAACCCTCGATGGAAAAGCTGATGCCGGAGGCTTTTCGCGAGCTT
TGCCGCATCTGCAGCGAACTCGAAATCCACTACCGCGACATGCAGGATATCGAATTCACCATCGAGCGCGGCAAGCTGTG
GATGCTGCAGACGCGTTCGGGCAAGCGCTCCACCCGGGCGGCGATGAAAATTGCCGTTGATATGGTCGATGAAAAAGTGA
TTACCGAGGAGCAGGCGGTGCTGCGCATCGAACCCTCCAGCCTCGACCAGCTGCTGCATCCGACGATCGATCCGCGCGTC
ACCCGCCAGGTGATCGGCTCCGGCCTGCCGGCCTCGCCGGGGGCCGCGACCGGCGCCATCGTCTTTACCGCCGAAGAGGC
CGTCGAGGCCGAGTCCGAAGGCCGCAAGGTCATCCTGCTCCGGGTCGAAACCAGCCCGGAGGATATTCACGGCATGCATG
CCGCCGAAGGCATTCTGACGACGCGCGGCGGCATGACCAGCCATGCGGCGGTCGTTGCCCGCGGCATGGGCATCCCCTGC
GTCGTCGGCGCCGGCACCATGCGCATCGATCAGCGCAACGAGCGGCTGCTCGGCATCGGCGTCACCCTGAAAAAGGGCGA
TATCATCACCATCGACGGTTCGGCCGGCCAGGTGCTGAAGGGCGAGGTGCCGATGATCCAGCCGGAGCTTTCGGGCGATT
TCGGCCGCATCATGGGCTGGGCCGATCGCGCCAGGCGCATGACGGTGCGCACCAATGCCGATACGCCGGCCGATGCGCGC
GCCGCCCGCTCCTTCGGCGCCGAAGGCATCGGCCTCTGCCGCACCGAGCACATGTTCTTCGAGGGCGAGCGCATCCATGT
GATGCGCGAGATGATCCTCGCCGTCGATGAGAAGGGCAGGCGGCTGGCGCTCGACAAGCTGCTGCCAATGCAGCGGCTGG
ATTTCACCGGCCTCTTCACCGTCATGCACGGCCTGCCGGTGACGATCCGCCTGCTCGATCCGCCGTTGCACGAATTCCTG
CCGAAGACCGATGACGAGGTCGCCGAAGTCGCCTTCGCCATGGGCATGGAGGCGATAGCCTTGCGCCAGCGTGTCGACGC
GCTGCACGAGTTCAATCCGATGCTTGGCCATCGTGGCTGCCGGCTGGCGATCTCCTATCCCGAAATCGTCGAGATGCAGG
CCCGCGCTATCTTCGAGGCGGCGGTTGCCGCAGCGAAGGAGACCGGGGCTGCCGTCGTGCCGGAGATCATGGTGCCGCTG
GTCGGCCTGCGCTCCGAGCTCGATTACGTCAAGGCGCGCATCGACGAGGTCGCCGGCGATGTGATGCGCGAGGCCGGCAT
GAAGATCGATTATCTCGTCGGCACGATGATCGAGCTGCCGCGCGCGGCCCTGCGCGCCCATGTGATTGCCGAAGCCGCCG
AATTCTTCTCCTTCGGCACCAACGACCTGACGCAGACCACCTTCGGCATCTCGCGCGACGACGCATCCGCCTTCATCCCC
ACCTATCAGCGCAAGGGCATCATCGAGCACGACCCGTTCATCTCGCTCGATTTCGACGGTGTCGGCGAATTGATCAGCAT
TGCCGCCGAGCGCGGAAGGCAGACCCGCAACGACATGAAGCTCGGCATTTGCGGCGAACACGGCGGCGACCCGGCCTCGA
TCCGCTTCTGCGAAACGATCGGGCTCGATTATGTCTCCTGCTCGCCGTTTCGCGTGCCGATCGCCAGACTGGCGGCGGCG
CAGGCGGTTATTTCAGGAGGTCTGAAAGACCTTGGGGGGAAATAG

Upstream 100 bases:

>100_bases
GCCGAAGAATTATTGTGCAACGCAACATTCCGCCGCATTTGTCGATTTTCATTTCAACACCAACGGATTAAGCTAATCCC
GGTTTGGTGTTGGGGGGAAG

Downstream 100 bases:

>100_bases
CCAGAGACCGGCAGCCCCCGGCCTCAATGATGATGCCGCCAGCGATCGCGGTAGATATAGGGCCCGTTCAGATCCCAGGC
CATGCTCTGCATCATCTCGG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 894; Mature: 893

Protein sequence:

>894_residues
MTKWVYTFGDGQAEGRARDHEILGGKGANLAEMCALGLPVPPGLTIIGDACNTYYRNGRHIEDGVKAEVRAGISGIEAIT
GRRFGAGSQPLLLSVRSGARVSMPGMMDTVLNLGLNDETVQALGHDAGDARFAWDSYRRFIQMYADVVMGLGNDAFEEIL
EDEKAKLGHETDTELSASEWQHIVFLYKKLIEEELEQEFPQDPEVQLWGAVGAVFSSWMSARAVTYRQLHNIPEGWGTAV
NIQAMVFGNLGNASATGVAFTRNPSTGEKALYGEFLVNAQGEDVVAGIRTPQSITEEGRLSSGSEKPSMEKLMPEAFREL
CRICSELEIHYRDMQDIEFTIERGKLWMLQTRSGKRSTRAAMKIAVDMVDEKVITEEQAVLRIEPSSLDQLLHPTIDPRV
TRQVIGSGLPASPGAATGAIVFTAEEAVEAESEGRKVILLRVETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGIPC
VVGAGTMRIDQRNERLLGIGVTLKKGDIITIDGSAGQVLKGEVPMIQPELSGDFGRIMGWADRARRMTVRTNADTPADAR
AARSFGAEGIGLCRTEHMFFEGERIHVMREMILAVDEKGRRLALDKLLPMQRLDFTGLFTVMHGLPVTIRLLDPPLHEFL
PKTDDEVAEVAFAMGMEAIALRQRVDALHEFNPMLGHRGCRLAISYPEIVEMQARAIFEAAVAAAKETGAAVVPEIMVPL
VGLRSELDYVKARIDEVAGDVMREAGMKIDYLVGTMIELPRAALRAHVIAEAAEFFSFGTNDLTQTTFGISRDDASAFIP
TYQRKGIIEHDPFISLDFDGVGELISIAAERGRQTRNDMKLGICGEHGGDPASIRFCETIGLDYVSCSPFRVPIARLAAA
QAVISGGLKDLGGK

Sequences:

>Translated_894_residues
MTKWVYTFGDGQAEGRARDHEILGGKGANLAEMCALGLPVPPGLTIIGDACNTYYRNGRHIEDGVKAEVRAGISGIEAIT
GRRFGAGSQPLLLSVRSGARVSMPGMMDTVLNLGLNDETVQALGHDAGDARFAWDSYRRFIQMYADVVMGLGNDAFEEIL
EDEKAKLGHETDTELSASEWQHIVFLYKKLIEEELEQEFPQDPEVQLWGAVGAVFSSWMSARAVTYRQLHNIPEGWGTAV
NIQAMVFGNLGNASATGVAFTRNPSTGEKALYGEFLVNAQGEDVVAGIRTPQSITEEGRLSSGSEKPSMEKLMPEAFREL
CRICSELEIHYRDMQDIEFTIERGKLWMLQTRSGKRSTRAAMKIAVDMVDEKVITEEQAVLRIEPSSLDQLLHPTIDPRV
TRQVIGSGLPASPGAATGAIVFTAEEAVEAESEGRKVILLRVETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGIPC
VVGAGTMRIDQRNERLLGIGVTLKKGDIITIDGSAGQVLKGEVPMIQPELSGDFGRIMGWADRARRMTVRTNADTPADAR
AARSFGAEGIGLCRTEHMFFEGERIHVMREMILAVDEKGRRLALDKLLPMQRLDFTGLFTVMHGLPVTIRLLDPPLHEFL
PKTDDEVAEVAFAMGMEAIALRQRVDALHEFNPMLGHRGCRLAISYPEIVEMQARAIFEAAVAAAKETGAAVVPEIMVPL
VGLRSELDYVKARIDEVAGDVMREAGMKIDYLVGTMIELPRAALRAHVIAEAAEFFSFGTNDLTQTTFGISRDDASAFIP
TYQRKGIIEHDPFISLDFDGVGELISIAAERGRQTRNDMKLGICGEHGGDPASIRFCETIGLDYVSCSPFRVPIARLAAA
QAVISGGLKDLGGK
>Mature_893_residues
TKWVYTFGDGQAEGRARDHEILGGKGANLAEMCALGLPVPPGLTIIGDACNTYYRNGRHIEDGVKAEVRAGISGIEAITG
RRFGAGSQPLLLSVRSGARVSMPGMMDTVLNLGLNDETVQALGHDAGDARFAWDSYRRFIQMYADVVMGLGNDAFEEILE
DEKAKLGHETDTELSASEWQHIVFLYKKLIEEELEQEFPQDPEVQLWGAVGAVFSSWMSARAVTYRQLHNIPEGWGTAVN
IQAMVFGNLGNASATGVAFTRNPSTGEKALYGEFLVNAQGEDVVAGIRTPQSITEEGRLSSGSEKPSMEKLMPEAFRELC
RICSELEIHYRDMQDIEFTIERGKLWMLQTRSGKRSTRAAMKIAVDMVDEKVITEEQAVLRIEPSSLDQLLHPTIDPRVT
RQVIGSGLPASPGAATGAIVFTAEEAVEAESEGRKVILLRVETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGIPCV
VGAGTMRIDQRNERLLGIGVTLKKGDIITIDGSAGQVLKGEVPMIQPELSGDFGRIMGWADRARRMTVRTNADTPADARA
ARSFGAEGIGLCRTEHMFFEGERIHVMREMILAVDEKGRRLALDKLLPMQRLDFTGLFTVMHGLPVTIRLLDPPLHEFLP
KTDDEVAEVAFAMGMEAIALRQRVDALHEFNPMLGHRGCRLAISYPEIVEMQARAIFEAAVAAAKETGAAVVPEIMVPLV
GLRSELDYVKARIDEVAGDVMREAGMKIDYLVGTMIELPRAALRAHVIAEAAEFFSFGTNDLTQTTFGISRDDASAFIPT
YQRKGIIEHDPFISLDFDGVGELISIAAERGRQTRNDMKLGICGEHGGDPASIRFCETIGLDYVSCSPFRVPIARLAAAQ
AVISGGLKDLGGK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=459, Percent_Identity=24.1830065359477, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1787994, Length=726, Percent_Identity=26.0330578512397, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1789193, Length=426, Percent_Identity=24.4131455399061, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI48994992, Length=449, Percent_Identity=24.2761692650334, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1788726, Length=447, Percent_Identity=22.1476510067114, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97383; Mature: 97252

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWVYTFGDGQAEGRARDHEILGGKGANLAEMCALGLPVPPGLTIIGDACNTYYRNGRH
CCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEECCHHHHHHCCCCC
IEDGVKAEVRAGISGIEAITGRRFGAGSQPLLLSVRSGARVSMPGMMDTVLNLGLNDETV
CCCCCHHHHHHCCHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHH
QALGHDAGDARFAWDSYRRFIQMYADVVMGLGNDAFEEILEDEKAKLGHETDTELSASEW
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
QHIVFLYKKLIEEELEQEFPQDPEVQLWGAVGAVFSSWMSARAVTYRQLHNIPEGWGTAV
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
NIQAMVFGNLGNASATGVAFTRNPSTGEKALYGEFLVNAQGEDVVAGIRTPQSITEEGRL
EEEEEEEECCCCCCCCCEEEECCCCCCCHHEEHHHEECCCCCCEEEECCCCHHHHHCCCC
SSGSEKPSMEKLMPEAFRELCRICSELEIHYRDMQDIEFTIERGKLWMLQTRSGKRSTRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHH
AMKIAVDMVDEKVITEEQAVLRIEPSSLDQLLHPTIDPRVTRQVIGSGLPASPGAATGAI
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEE
VFTAEEAVEAESEGRKVILLRVETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGIPC
EEEEHHHHHHHCCCCEEEEEEEECCCHHHCCHHHHCCEEEECCCCCHHHHHHHCCCCCCE
VVGAGTMRIDQRNERLLGIGVTLKKGDIITIDGSAGQVLKGEVPMIQPELSGDFGRIMGW
EECCCEEEEECCCCEEEEEEEEEECCCEEEEECCCCCEEECCCCEECCCCCCCHHHHHHH
ADRARRMTVRTNADTPADARAARSFGAEGIGLCRTEHMFFEGERIHVMREMILAVDEKGR
HHHHCEEEEEECCCCCHHHHHHHHCCCCCCCEEEECCEEECCCHHHHHHHHHHHHCCCCC
RLALDKLLPMQRLDFTGLFTVMHGLPVTIRLLDPPLHEFLPKTDDEVAEVAFAMGMEAIA
EEEHHHHCCHHHCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHH
LRQRVDALHEFNPMLGHRGCRLAISYPEIVEMQARAIFEAAVAAAKETGAAVVPEIMVPL
HHHHHHHHHHCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGLRSELDYVKARIDEVAGDVMREAGMKIDYLVGTMIELPRAALRAHVIAEAAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NDLTQTTFGISRDDASAFIPTYQRKGIIEHDPFISLDFDGVGELISIAAERGRQTRNDMK
CCHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCE
LGICGEHGGDPASIRFCETIGLDYVSCSPFRVPIARLAAAQAVISGGLKDLGGK
EEECCCCCCCCCCEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
TKWVYTFGDGQAEGRARDHEILGGKGANLAEMCALGLPVPPGLTIIGDACNTYYRNGRH
CCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEECCHHHHHHCCCCC
IEDGVKAEVRAGISGIEAITGRRFGAGSQPLLLSVRSGARVSMPGMMDTVLNLGLNDETV
CCCCCHHHHHHCCHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHH
QALGHDAGDARFAWDSYRRFIQMYADVVMGLGNDAFEEILEDEKAKLGHETDTELSASEW
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
QHIVFLYKKLIEEELEQEFPQDPEVQLWGAVGAVFSSWMSARAVTYRQLHNIPEGWGTAV
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
NIQAMVFGNLGNASATGVAFTRNPSTGEKALYGEFLVNAQGEDVVAGIRTPQSITEEGRL
EEEEEEEECCCCCCCCCEEEECCCCCCCHHEEHHHEECCCCCCEEEECCCCHHHHHCCCC
SSGSEKPSMEKLMPEAFRELCRICSELEIHYRDMQDIEFTIERGKLWMLQTRSGKRSTRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHH
AMKIAVDMVDEKVITEEQAVLRIEPSSLDQLLHPTIDPRVTRQVIGSGLPASPGAATGAI
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEE
VFTAEEAVEAESEGRKVILLRVETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGIPC
EEEEHHHHHHHCCCCEEEEEEEECCCHHHCCHHHHCCEEEECCCCCHHHHHHHCCCCCCE
VVGAGTMRIDQRNERLLGIGVTLKKGDIITIDGSAGQVLKGEVPMIQPELSGDFGRIMGW
EECCCEEEEECCCCEEEEEEEEEECCCEEEEECCCCCEEECCCCEECCCCCCCHHHHHHH
ADRARRMTVRTNADTPADARAARSFGAEGIGLCRTEHMFFEGERIHVMREMILAVDEKGR
HHHHCEEEEEECCCCCHHHHHHHHCCCCCCCEEEECCEEECCCHHHHHHHHHHHHCCCCC
RLALDKLLPMQRLDFTGLFTVMHGLPVTIRLLDPPLHEFLPKTDDEVAEVAFAMGMEAIA
EEEHHHHCCHHHCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHH
LRQRVDALHEFNPMLGHRGCRLAISYPEIVEMQARAIFEAAVAAAKETGAAVVPEIMVPL
HHHHHHHHHHCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGLRSELDYVKARIDEVAGDVMREAGMKIDYLVGTMIELPRAALRAHVIAEAAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NDLTQTTFGISRDDASAFIPTYQRKGIIEHDPFISLDFDGVGELISIAAERGRQTRNDMK
CCHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCE
LGICGEHGGDPASIRFCETIGLDYVSCSPFRVPIARLAAAQAVISGGLKDLGGK
EEECCCCCCCCCCEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]