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The map label for this gene is hipO [H]

Identifier: 209548234

GI number: 209548234

Start: 650276

End: 651436

Strand: Reverse

Name: hipO [H]

Synonym: Rleg2_0628

Alternate gene names: 209548234

Gene position: 651436-650276 (Counterclockwise)

Preceding gene: 209548235

Following gene: 209548233

Centisome position: 14.36

GC content: 64.43

Gene sequence:

>1161_bases
ATGAGCGTCGACAGCCGGAAGCTGGAACAGGACATGACCGCCTGGAGGCGCGACCTTCACAGCCATCCCGAATTCGGCTT
CGACGAGAAGCGGACGGCGGCTTTCGTCGCGCGGAAATTGCGGGAGTTCGGGCTCGACGAGGTTGTCGAAGGTGTCGGCG
GCACAGGGATTGTCGGAACGCTCCGGCGTGGCGGCGGCAACCGCTCCATTGCGCTGCGCGCCGATATGGATGCCTTGAGG
ATTGCCGAACAGGGCGATCGGCCCTATCGATCGCAGACCGCCGGCGTGATGCATGCCTGCGGCCATGACGGGCATACGGC
AATGCTACTTGGCGCCGCCCAGATGCTGTCTGAGGACGGCAATTTCGACGGCACGGTCCGCTTCATCTTTCAGCCGGCCG
AGGAATGGGGCAAAGGCGCCTTGGCGATGCTCGACGATGGGCTGATGACGCGCTTTCCCTTCGACGAGATCTACGGCCTG
CACAATATGCCCGGCCTTCCGGTCGGTGTTTTCCAGACCCGCGCCGGCGCGATCATGTCGGCTGAGGATAATTTCGAGAT
CGTGCTCAAGGGCGTCGGCGGCCACGCCGCGCGTCCGCACTCCGGCAACGAGGTCCTGGTCGCCGCCTGCGCTTTGGTGA
CGAACCTCCAGACCATCGTTTCCCGGCGGCTCGATCCGACCGATATCGGCGTCGTCTCCGTCACCGAGCTTCTGACCGAC
GGCACCCGCAACGCTTTGCCTGGGCTGGCCCGCATCCTCGGCGATGCCCGCAGCTTCCGCCCCGAAGTCAGCGCGGCGAT
CGAAAAGCACATGCGCCGCATCGCCGAAGGCACCGCGCTTGCCTACAATGTCTCGGCCGAGGTGAACTATACGAGAGAAT
TCGTTCCCCTGCTCAACGACGCCGCCCTGGCCGAGGAAGCCTTTGCCGCAGCCCGCAGCGTCTTCCCGTCCGAGAACGTC
AAGGTCCGGCGCGAGCCGATGACCGGATCGGAAGACTTCGCCCGCTTCCTCGACCATGTCCCCGGCTGCTTCGTCTTCCT
CGGCAATGGCGAAGGTTCCGCGCCGCTGCACAATCCGAATTACGATTTCAACGACGCCGGACTGATCCACGGTGCCAAAT
TCCACGCGAGCATCGTGCGTCGGCGGTTGAACGCCAGCTGA

Upstream 100 bases:

>100_bases
GTCACAATCCGAAGGAATTCACATCAGATACGGAGCTTGTCGCGGGGGCGAATATCCTCCTCGACGTCGTTTGCGGACTT
GCAACAGGGGAACTGCCGAG

Downstream 100 bases:

>100_bases
TGATCGGCCGCGGCCCGCCGTCCTTGACCCGGTTTTCGGATGGCAGCGGGTTACGCGAATGATGAGGCATTTGCAACCGC
CTCCGCCAAACCATTGACCA

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSVDSRKLEQDMTAWRRDLHSHPEFGFDEKRTAAFVARKLREFGLDEVVEGVGGTGIVGTLRRGGGNRSIALRADMDALR
IAEQGDRPYRSQTAGVMHACGHDGHTAMLLGAAQMLSEDGNFDGTVRFIFQPAEEWGKGALAMLDDGLMTRFPFDEIYGL
HNMPGLPVGVFQTRAGAIMSAEDNFEIVLKGVGGHAARPHSGNEVLVAACALVTNLQTIVSRRLDPTDIGVVSVTELLTD
GTRNALPGLARILGDARSFRPEVSAAIEKHMRRIAEGTALAYNVSAEVNYTREFVPLLNDAALAEEAFAAARSVFPSENV
KVRREPMTGSEDFARFLDHVPGCFVFLGNGEGSAPLHNPNYDFNDAGLIHGAKFHASIVRRRLNAS

Sequences:

>Translated_386_residues
MSVDSRKLEQDMTAWRRDLHSHPEFGFDEKRTAAFVARKLREFGLDEVVEGVGGTGIVGTLRRGGGNRSIALRADMDALR
IAEQGDRPYRSQTAGVMHACGHDGHTAMLLGAAQMLSEDGNFDGTVRFIFQPAEEWGKGALAMLDDGLMTRFPFDEIYGL
HNMPGLPVGVFQTRAGAIMSAEDNFEIVLKGVGGHAARPHSGNEVLVAACALVTNLQTIVSRRLDPTDIGVVSVTELLTD
GTRNALPGLARILGDARSFRPEVSAAIEKHMRRIAEGTALAYNVSAEVNYTREFVPLLNDAALAEEAFAAARSVFPSENV
KVRREPMTGSEDFARFLDHVPGCFVFLGNGEGSAPLHNPNYDFNDAGLIHGAKFHASIVRRRLNAS
>Mature_385_residues
SVDSRKLEQDMTAWRRDLHSHPEFGFDEKRTAAFVARKLREFGLDEVVEGVGGTGIVGTLRRGGGNRSIALRADMDALRI
AEQGDRPYRSQTAGVMHACGHDGHTAMLLGAAQMLSEDGNFDGTVRFIFQPAEEWGKGALAMLDDGLMTRFPFDEIYGLH
NMPGLPVGVFQTRAGAIMSAEDNFEIVLKGVGGHAARPHSGNEVLVAACALVTNLQTIVSRRLDPTDIGVVSVTELLTDG
TRNALPGLARILGDARSFRPEVSAAIEKHMRRIAEGTALAYNVSAEVNYTREFVPLLNDAALAEEAFAAARSVFPSENVK
VRREPMTGSEDFARFLDHVPGCFVFLGNGEGSAPLHNPNYDFNDAGLIHGAKFHASIVRRRLNAS

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=428, Percent_Identity=30.3738317757009, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41872; Mature: 41741

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDSRKLEQDMTAWRRDLHSHPEFGFDEKRTAAFVARKLREFGLDEVVEGVGGTGIVGT
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHH
LRRGGGNRSIALRADMDALRIAEQGDRPYRSQTAGVMHACGHDGHTAMLLGAAQMLSEDG
HHCCCCCCEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
NFDGTVRFIFQPAEEWGKGALAMLDDGLMTRFPFDEIYGLHNMPGLPVGVFQTRAGAIMS
CCCCEEEEEEECHHHHCCCCEEHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHCCCCEEE
AEDNFEIVLKGVGGHAARPHSGNEVLVAACALVTNLQTIVSRRLDPTDIGVVSVTELLTD
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GTRNALPGLARILGDARSFRPEVSAAIEKHMRRIAEGTALAYNVSAEVNYTREFVPLLND
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
AALAEEAFAAARSVFPSENVKVRREPMTGSEDFARFLDHVPGCFVFLGNGEGSAPLHNPN
HHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
YDFNDAGLIHGAKFHASIVRRRLNAS
CCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVDSRKLEQDMTAWRRDLHSHPEFGFDEKRTAAFVARKLREFGLDEVVEGVGGTGIVGT
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHH
LRRGGGNRSIALRADMDALRIAEQGDRPYRSQTAGVMHACGHDGHTAMLLGAAQMLSEDG
HHCCCCCCEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
NFDGTVRFIFQPAEEWGKGALAMLDDGLMTRFPFDEIYGLHNMPGLPVGVFQTRAGAIMS
CCCCEEEEEEECHHHHCCCCEEHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHCCCCEEE
AEDNFEIVLKGVGGHAARPHSGNEVLVAACALVTNLQTIVSRRLDPTDIGVVSVTELLTD
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GTRNALPGLARILGDARSFRPEVSAAIEKHMRRIAEGTALAYNVSAEVNYTREFVPLLND
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
AALAEEAFAAARSVFPSENVKVRREPMTGSEDFARFLDHVPGCFVFLGNGEGSAPLHNPN
HHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
YDFNDAGLIHGAKFHASIVRRRLNAS
CCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]