Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 209548141

GI number: 209548141

Start: 549623

End: 550906

Strand: Direct

Name: purD [H]

Synonym: Rleg2_0535

Alternate gene names: 209548141

Gene position: 549623-550906 (Clockwise)

Preceding gene: 209548140

Following gene: 209548142

Centisome position: 12.11

GC content: 64.1

Gene sequence:

>1284_bases
ATGAAGGTTCTGTTGATCGGATCGGGCGGACGCGAGCATGCGCTCGCTTGGAAGCTGGCTCAATCGCCGTTGATGACGGA
ATTTTACGCCGCGCCCGGCAATCCCGGCATTGCCGAACACGCCATGCTGGTGCCCGTCAACATCGAGGATCATGAAGCGG
TCGCCGCCTTCTGTAAGGACAAGGCGATCGATTTCGTCGTCGTTGGCCCCGAGGCGCCGCTGGTGGCCGGCCTTGCCGAC
CGGCTGCGCGCCGATGGCCTTGTGGTTTTCGGACCCTCGGCCGCGGCCGCCCAGCTCGAGGGTTCCAAGGGCTTCACCAA
GGATATCTGCGCCCGCTACGGCATTCCGACTGGCGCCTATCAGCGCTTCAACAATGCGCCGAAGGCCAAAGCCTATATTC
GCGATCAGGGAGTGCCGATCGTCGTCAAGGCTGATGGCCTTGCCGCCGGCAAGGGCGTGACGGTGGCGATGACGCTGGAT
GAAGCCTTGGCCGCCGTCGACGACTGTTTCGAAGGCGCCTTTGGCGCTGCCGGCGCCGAAGTCGTCGTCGAAGCCTATCT
CGACGGTGAGGAGGCGAGTTTCTTCTGCCTCTGCGATGGGAAGAATGCGCTGCCGCTCGCAACCGCCCAGGATCACAAGC
GGGTGGGCGAGGGCGATACCGGCGTCAATACCGGCGGCATGGGCGCCTATTCGCCGGCGCCGGTGATGACCGCCGAAATG
GTCGAGCGCACCATGAAGGAGATCATCGAGCCGACGATGCGCGGCATGGCCGAGAGCGGCCATCCCTTCTCCGGCGTATT
TTTCGCCGGGCTGATGATCACCAGCAGGGGGCCGGAGCTGATCGAATACAATGTCCGCTTCGGCGATCCCGAATGTCAGG
TGATGATGATGCGGCTGAAGAGCGATCTGCTGCCGCTGCTGCTCGCCACTGCCAACGGCACGCTCGATCAGATGAGCGCC
GAATGGAGCGACGATCCGGCCCTGACGGTGGTCATGGCCTCGAAGGGCTATCCCGCCGCTTATGAAAAGAACACGCCGAT
CCTCTCCCTGCCTGAGGCAGGCGAGGGCGAGAAGGTGTTTCATGCCGGCACGGGCCTGAAGGACGGCGCGCTGGTTGCGA
CCGGTGGCCGCGTGCTGAACGTCACTGCGTCAGGCGGCACCGTCGCCGAGGCCAAGGACCGCGCCTACGCGCTGCTCGAC
AGGGTCAAGTGGGACAACGGCTTTTCCAGGCGCGACATCGGCTGGCGGGCGATCGAGCGTGAAACAGGCTCGGACAAAGT
ATAA

Upstream 100 bases:

>100_bases
GCGGTGGCGGAGGCGGGGGAGGCGGGGGCTGGTAAGCTTTCCCGCTTGACCTTTCGCCCCTTGGCCCTTAACCGCCAGAG
GCAAAAGGAAAGGGTGGCGC

Downstream 100 bases:

>100_bases
ACCGCCACGTTCTTTAAATTTTTACCCTTTCCCCTGACGGGATGGAAACAAAGCTGCGCTATACCGCTCTCATAGTGAGA
CGGAGGTGCGTTGATGCGTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKVLLIGSGGREHALAWKLAQSPLMTEFYAAPGNPGIAEHAMLVPVNIEDHEAVAAFCKDKAIDFVVVGPEAPLVAGLAD
RLRADGLVVFGPSAAAAQLEGSKGFTKDICARYGIPTGAYQRFNNAPKAKAYIRDQGVPIVVKADGLAAGKGVTVAMTLD
EALAAVDDCFEGAFGAAGAEVVVEAYLDGEEASFFCLCDGKNALPLATAQDHKRVGEGDTGVNTGGMGAYSPAPVMTAEM
VERTMKEIIEPTMRGMAESGHPFSGVFFAGLMITSRGPELIEYNVRFGDPECQVMMMRLKSDLLPLLLATANGTLDQMSA
EWSDDPALTVVMASKGYPAAYEKNTPILSLPEAGEGEKVFHAGTGLKDGALVATGGRVLNVTASGGTVAEAKDRAYALLD
RVKWDNGFSRRDIGWRAIERETGSDKV

Sequences:

>Translated_427_residues
MKVLLIGSGGREHALAWKLAQSPLMTEFYAAPGNPGIAEHAMLVPVNIEDHEAVAAFCKDKAIDFVVVGPEAPLVAGLAD
RLRADGLVVFGPSAAAAQLEGSKGFTKDICARYGIPTGAYQRFNNAPKAKAYIRDQGVPIVVKADGLAAGKGVTVAMTLD
EALAAVDDCFEGAFGAAGAEVVVEAYLDGEEASFFCLCDGKNALPLATAQDHKRVGEGDTGVNTGGMGAYSPAPVMTAEM
VERTMKEIIEPTMRGMAESGHPFSGVFFAGLMITSRGPELIEYNVRFGDPECQVMMMRLKSDLLPLLLATANGTLDQMSA
EWSDDPALTVVMASKGYPAAYEKNTPILSLPEAGEGEKVFHAGTGLKDGALVATGGRVLNVTASGGTVAEAKDRAYALLD
RVKWDNGFSRRDIGWRAIERETGSDKV
>Mature_427_residues
MKVLLIGSGGREHALAWKLAQSPLMTEFYAAPGNPGIAEHAMLVPVNIEDHEAVAAFCKDKAIDFVVVGPEAPLVAGLAD
RLRADGLVVFGPSAAAAQLEGSKGFTKDICARYGIPTGAYQRFNNAPKAKAYIRDQGVPIVVKADGLAAGKGVTVAMTLD
EALAAVDDCFEGAFGAAGAEVVVEAYLDGEEASFFCLCDGKNALPLATAQDHKRVGEGDTGVNTGGMGAYSPAPVMTAEM
VERTMKEIIEPTMRGMAESGHPFSGVFFAGLMITSRGPELIEYNVRFGDPECQVMMMRLKSDLLPLLLATANGTLDQMSA
EWSDDPALTVVMASKGYPAAYEKNTPILSLPEAGEGEKVFHAGTGLKDGALVATGGRVLNVTASGGTVAEAKDRAYALLD
RVKWDNGFSRRDIGWRAIERETGSDKV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=49.645390070922, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=49.645390070922, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=49.645390070922, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=49.645390070922, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=58.07962529274, Blast_Score=476, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17567511, Length=419, Percent_Identity=41.0501193317422, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=39.5505617977528, Blast_Score=308, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24582400, Length=435, Percent_Identity=42.2988505747126, Blast_Score=304, Evalue=8e-83,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=42.5581395348837, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44981; Mature: 44981

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLIGSGGREHALAWKLAQSPLMTEFYAAPGNPGIAEHAMLVPVNIEDHEAVAAFCKD
CEEEEEECCCCCCCEEEHHHCCCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHC
KAIDFVVVGPEAPLVAGLADRLRADGLVVFGPSAAAAQLEGSKGFTKDICARYGIPTGAY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCEEECCCCCCHHHHHHHCCCCCHHH
QRFNNAPKAKAYIRDQGVPIVVKADGLAAGKGVTVAMTLDEALAAVDDCFEGAFGAAGAE
HHHCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHH
VVVEAYLDGEEASFFCLCDGKNALPLATAQDHKRVGEGDTGVNTGGMGAYSPAPVMTAEM
EEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
VERTMKEIIEPTMRGMAESGHPFSGVFFAGLMITSRGPELIEYNVRFGDPECQVMMMRLK
HHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHH
SDLLPLLLATANGTLDQMSAEWSDDPALTVVMASKGYPAAYEKNTPILSLPEAGEGEKVF
HHHHHEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCEEE
HAGTGLKDGALVATGGRVLNVTASGGTVAEAKDRAYALLDRVKWDNGFSRRDIGWRAIER
EECCCCCCCEEEEECCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEC
ETGSDKV
CCCCCCC
>Mature Secondary Structure
MKVLLIGSGGREHALAWKLAQSPLMTEFYAAPGNPGIAEHAMLVPVNIEDHEAVAAFCKD
CEEEEEECCCCCCCEEEHHHCCCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHC
KAIDFVVVGPEAPLVAGLADRLRADGLVVFGPSAAAAQLEGSKGFTKDICARYGIPTGAY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCEEECCCCCCHHHHHHHCCCCCHHH
QRFNNAPKAKAYIRDQGVPIVVKADGLAAGKGVTVAMTLDEALAAVDDCFEGAFGAAGAE
HHHCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHH
VVVEAYLDGEEASFFCLCDGKNALPLATAQDHKRVGEGDTGVNTGGMGAYSPAPVMTAEM
EEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
VERTMKEIIEPTMRGMAESGHPFSGVFFAGLMITSRGPELIEYNVRFGDPECQVMMMRLK
HHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHH
SDLLPLLLATANGTLDQMSAEWSDDPALTVVMASKGYPAAYEKNTPILSLPEAGEGEKVF
HHHHHEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCEEE
HAGTGLKDGALVATGGRVLNVTASGGTVAEAKDRAYALLDRVKWDNGFSRRDIGWRAIER
EECCCCCCCEEEEECCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEC
ETGSDKV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]