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The map label for this gene is rbsB [H]

Identifier: 209548100

GI number: 209548100

Start: 507079

End: 507996

Strand: Direct

Name: rbsB [H]

Synonym: Rleg2_0494

Alternate gene names: 209548100

Gene position: 507079-507996 (Clockwise)

Preceding gene: 209548099

Following gene: 209548103

Centisome position: 11.17

GC content: 57.73

Gene sequence:

>918_bases
ATGAAACCGCAATCCCTGCTGGGAGCCCTTGCGCTTTTGGCTGTCGCTGTAGTCCCGTCCTTGGCGCAGGAGCCGATCAA
GCTCGGCTTTATAACCAAATTCCCGGTACCGTTCTTCGCAACAATGGAGAACGCAGCCAAGGATTATGCAAAGAGAAATC
CCGGCGTCGAGATCATTTACGGGCAGGGAACCTCCGCAACCGACATCGAGGGCCAGATTGCACAGATCGAGTCCATGGTG
ACACGAGGCGTGCAGGGCATTGCGCTCACGCCCGTCGATCCCACCGTGTCCACGGCCCTGGACAAGGCGGTGGCTTCAGG
CGTCAAGGTCGTCCTGATGGATAACAATATTCCGGATTGGAAAGGCAGGACGGCGCTTGCCACGACCAATAATTTTGCCG
CGGGCAAGATCGCCGGCGAATATCTCAAAACGGTTCTTAAAGCTGGCGATACGCTCGGGATTCTCGAGGGTGTGCCCGGT
GTGCCAGCGCTTGATGACCGTGTGAAGGGTATGCTTGAAGGGCTGCAGGGCCTTGACGTCAAGATCGTCGGGAAAGGCGC
AACAAACTGCACCGAGGAACTCGGGATCAGTGTCGCCGAAGATCTGCTGACGAAGAACCCGGACCTCAAGGCCATTTACG
CCGCTTGCGGCCCGCCCGCTGCTGGCGCTGCACGCGCAATCAAGAATTCCGGCACTGCCAATGACAAGATCGTGTTGGTC
GGTTTCGACTTTTGCTGCGGCGAAGAGGAGGCGCTGAAGAGTGGAGTCGAGGATGCTTCGGTGGCGCAGTTCCCCACCAA
GATGGCTGAACTCGGTGTGGATGCGCTGGTAAAATCGATTCGCGGAGAGAAGGTGGAGTCTTTGATCGATTCCGGGGCCG
CGCTCGTGACGCCTGAGAATATGGCGCAATTCAAGTAA

Upstream 100 bases:

>100_bases
ATTGTCGGCGCTGAGGCTTAGGGGAGATCGCTGCTTCGATAGGGCAGTGGACAACGAGATCGCTCTAGAGGAGGGCGATC
GAAACATGGGAGGTGTTATG

Downstream 100 bases:

>100_bases
ACCGTTGCCTCTCTGTCCAATTTGTAGAGAGGGCATATCGACCTTGTTGGTGCTTAGCTAACGGCCCGTCCTTCGTGGGC
GGGCCGGACCTGTGGCCCCT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKPQSLLGALALLAVAVVPSLAQEPIKLGFITKFPVPFFATMENAAKDYAKRNPGVEIIYGQGTSATDIEGQIAQIESMV
TRGVQGIALTPVDPTVSTALDKAVASGVKVVLMDNNIPDWKGRTALATTNNFAAGKIAGEYLKTVLKAGDTLGILEGVPG
VPALDDRVKGMLEGLQGLDVKIVGKGATNCTEELGISVAEDLLTKNPDLKAIYAACGPPAAGAARAIKNSGTANDKIVLV
GFDFCCGEEEALKSGVEDASVAQFPTKMAELGVDALVKSIRGEKVESLIDSGAALVTPENMAQFK

Sequences:

>Translated_305_residues
MKPQSLLGALALLAVAVVPSLAQEPIKLGFITKFPVPFFATMENAAKDYAKRNPGVEIIYGQGTSATDIEGQIAQIESMV
TRGVQGIALTPVDPTVSTALDKAVASGVKVVLMDNNIPDWKGRTALATTNNFAAGKIAGEYLKTVLKAGDTLGILEGVPG
VPALDDRVKGMLEGLQGLDVKIVGKGATNCTEELGISVAEDLLTKNPDLKAIYAACGPPAAGAARAIKNSGTANDKIVLV
GFDFCCGEEEALKSGVEDASVAQFPTKMAELGVDALVKSIRGEKVESLIDSGAALVTPENMAQFK
>Mature_305_residues
MKPQSLLGALALLAVAVVPSLAQEPIKLGFITKFPVPFFATMENAAKDYAKRNPGVEIIYGQGTSATDIEGQIAQIESMV
TRGVQGIALTPVDPTVSTALDKAVASGVKVVLMDNNIPDWKGRTALATTNNFAAGKIAGEYLKTVLKAGDTLGILEGVPG
VPALDDRVKGMLEGLQGLDVKIVGKGATNCTEELGISVAEDLLTKNPDLKAIYAACGPPAAGAARAIKNSGTANDKIVLV
GFDFCCGEEEALKSGVEDASVAQFPTKMAELGVDALVKSIRGEKVESLIDSGAALVTPENMAQFK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=290, Percent_Identity=26.551724137931, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790526, Length=258, Percent_Identity=27.1317829457364, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 31600; Mature: 31600

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQSLLGALALLAVAVVPSLAQEPIKLGFITKFPVPFFATMENAAKDYAKRNPGVEIIY
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEE
GQGTSATDIEGQIAQIESMVTRGVQGIALTPVDPTVSTALDKAVASGVKVVLMDNNIPDW
CCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCC
KGRTALATTNNFAAGKIAGEYLKTVLKAGDTLGILEGVPGVPALDDRVKGMLEGLQGLDV
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHCCCCE
KIVGKGATNCTEELGISVAEDLLTKNPDLKAIYAACGPPAAGAARAIKNSGTANDKIVLV
EEEECCCCCHHHHHCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
GFDFCCGEEEALKSGVEDASVAQFPTKMAELGVDALVKSIRGEKVESLIDSGAALVTPEN
EECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHCCCEEECCHH
MAQFK
HHCCC
>Mature Secondary Structure
MKPQSLLGALALLAVAVVPSLAQEPIKLGFITKFPVPFFATMENAAKDYAKRNPGVEIIY
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEE
GQGTSATDIEGQIAQIESMVTRGVQGIALTPVDPTVSTALDKAVASGVKVVLMDNNIPDW
CCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCC
KGRTALATTNNFAAGKIAGEYLKTVLKAGDTLGILEGVPGVPALDDRVKGMLEGLQGLDV
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCHHHHHHHHHHHHCCCCE
KIVGKGATNCTEELGISVAEDLLTKNPDLKAIYAACGPPAAGAARAIKNSGTANDKIVLV
EEEECCCCCHHHHHCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
GFDFCCGEEEALKSGVEDASVAQFPTKMAELGVDALVKSIRGEKVESLIDSGAALVTPEN
EECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHCCCEEECCHH
MAQFK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]