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The map label for this gene is pmbA [H]

Identifier: 209548091

GI number: 209548091

Start: 498147

End: 499493

Strand: Direct

Name: pmbA [H]

Synonym: Rleg2_0485

Alternate gene names: 209548091

Gene position: 498147-499493 (Clockwise)

Preceding gene: 209548089

Following gene: 209548092

Centisome position: 10.98

GC content: 65.18

Gene sequence:

>1347_bases
ATGTCCTCTGAAATCGATTCCTCGACACTTCTTTCCCGCGCAAGTCAATTGATCGATCTGGCCAGGAAGGCAGGGGCCGA
TGCCGCCGACGCCGTCGTCGTCCGGTCGCGCTCACAATCGGTCAGCGTCCGCCTCGGCAAGGTCGAGGGCACGGAATCTT
CCGAAAGCGACGATTTTTCGCTGCGTGTCTTTATCGGCAAGCGCGTCGCCAGCGTTTCGGCCAATCCGGGCTTCGATCTC
CAGGCGCTGGCCGAACGCGCCGTCGCCATGGCCAAGGTCTCGCCGGAAGACCCCTTCGCCTGCCTCGCGGATGAGGCGAG
CCTGGCGAAATCCTATCCCGACCTGCAATTGCTCGATACCACCGAGGTCTCCTCCGAGATGCTGCGCGAGGCAGGCCTTG
CCGCCGAAGCCGCGGCCCTTGCGGTCAAGGGTGTCACCAATTCCTCCGGCGCCGGTGCCTCGGCCGGCATGGGCGGCTTG
GTTCTTGCCACCTCGCACGGTTTCGAAGGCAGCTACATGGCCTCGCGTTTCGGCCATTCCGTCAGCGTCATCGCCGGCGA
AGGCACCGGCATGGAGCGCGATTATGATTTCGACAGCCGGCTCTATTATGCCGAGCTCGACGATCCCGCCGACATCGGCC
GCCGCGCCGGCGAACGGGTGATCAAGCGCATCAATCCGCGCCAGGTGCCGACCGGCAAGGACGTCACCGTCATCTTCGAC
CCGCGCGTCGCCCGCGGCTTCGTCGGCCATATCGCCGGCGCGATCAATGGTGCCGCCGTCGCCCGTAAGTCGAGCTTCCT
GCGCGACAGGATGGGTCAGCAGGTGCTGAAATCGGGCCTGTCGATCACCGACGACCCATTGATCGTGCGCGGCCCCTCCT
CGCGGCCCTTCGACGGCGAGGGCGTATCGGGCGAGCGGATGGTGATGATCGAGGACGGCGTCTTGAAGCACTGGTTCCTG
TCGACCTCGACCGGTCGCGAGCTCGGCCTTCAGACCAACGGCCGCGGCGTGCGCGGCGGCACTGCCGTCTCGCCGTCCTC
CACCAATCTTGCCCTTGAACCCGGCGACATCACGCCGGAGGAACTGATCCGCAGCGTCGGCAACGGTTTTTATGTCACCG
AGCTGATCGGCCACGGCGTCAATATGATCACCGGCGAATACAGCCGCGGCGCCACCGGCTTCTGGATCGAGAACGGCGAA
CTGACCTTCCCGGTCTCCGAGGTGACGATCGCCTCGAACCTCAAGGACATGTTCATGCGGCTGACGCCGGCAAACGACAT
CGACCGCAAGTTCGGCGTCGCGGCCCCGACCTTCGCCATCGAAGGCATGACGCTGGCAGGGCGCTAG

Upstream 100 bases:

>100_bases
GGCGGGGAACAGGCCGGCAGCCCCGACAAAGAATCGGCGGCGGCGGCCTTGATGCTTGGGTGAGTGCACAATAAATGGAA
GCCGAAGGAAAGGCCAAATC

Downstream 100 bases:

>100_bases
AGCATGATGCCGAAAAGTGTGTGCGGTTTTCGGACGACATCATGCTCAAACGAAAGTTGGACGGATTGATGCGATGAGCG
ATAGGGAAAAGGCCCGCTGG

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSSEIDSSTLLSRASQLIDLARKAGADAADAVVVRSRSQSVSVRLGKVEGTESSESDDFSLRVFIGKRVASVSANPGFDL
QALAERAVAMAKVSPEDPFACLADEASLAKSYPDLQLLDTTEVSSEMLREAGLAAEAAALAVKGVTNSSGAGASAGMGGL
VLATSHGFEGSYMASRFGHSVSVIAGEGTGMERDYDFDSRLYYAELDDPADIGRRAGERVIKRINPRQVPTGKDVTVIFD
PRVARGFVGHIAGAINGAAVARKSSFLRDRMGQQVLKSGLSITDDPLIVRGPSSRPFDGEGVSGERMVMIEDGVLKHWFL
STSTGRELGLQTNGRGVRGGTAVSPSSTNLALEPGDITPEELIRSVGNGFYVTELIGHGVNMITGEYSRGATGFWIENGE
LTFPVSEVTIASNLKDMFMRLTPANDIDRKFGVAAPTFAIEGMTLAGR

Sequences:

>Translated_448_residues
MSSEIDSSTLLSRASQLIDLARKAGADAADAVVVRSRSQSVSVRLGKVEGTESSESDDFSLRVFIGKRVASVSANPGFDL
QALAERAVAMAKVSPEDPFACLADEASLAKSYPDLQLLDTTEVSSEMLREAGLAAEAAALAVKGVTNSSGAGASAGMGGL
VLATSHGFEGSYMASRFGHSVSVIAGEGTGMERDYDFDSRLYYAELDDPADIGRRAGERVIKRINPRQVPTGKDVTVIFD
PRVARGFVGHIAGAINGAAVARKSSFLRDRMGQQVLKSGLSITDDPLIVRGPSSRPFDGEGVSGERMVMIEDGVLKHWFL
STSTGRELGLQTNGRGVRGGTAVSPSSTNLALEPGDITPEELIRSVGNGFYVTELIGHGVNMITGEYSRGATGFWIENGE
LTFPVSEVTIASNLKDMFMRLTPANDIDRKFGVAAPTFAIEGMTLAGR
>Mature_447_residues
SSEIDSSTLLSRASQLIDLARKAGADAADAVVVRSRSQSVSVRLGKVEGTESSESDDFSLRVFIGKRVASVSANPGFDLQ
ALAERAVAMAKVSPEDPFACLADEASLAKSYPDLQLLDTTEVSSEMLREAGLAAEAAALAVKGVTNSSGAGASAGMGGLV
LATSHGFEGSYMASRFGHSVSVIAGEGTGMERDYDFDSRLYYAELDDPADIGRRAGERVIKRINPRQVPTGKDVTVIFDP
RVARGFVGHIAGAINGAAVARKSSFLRDRMGQQVLKSGLSITDDPLIVRGPSSRPFDGEGVSGERMVMIEDGVLKHWFLS
TSTGRELGLQTNGRGVRGGTAVSPSSTNLALEPGDITPEELIRSVGNGFYVTELIGHGVNMITGEYSRGATGFWIENGEL
TFPVSEVTIASNLKDMFMRLTPANDIDRKFGVAAPTFAIEGMTLAGR

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=428, Percent_Identity=38.3177570093458, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1789640, Length=218, Percent_Identity=28.8990825688073, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47281; Mature: 47150

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEIDSSTLLSRASQLIDLARKAGADAADAVVVRSRSQSVSVRLGKVEGTESSESDDFS
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCEEEEEEEEECCCCCCCCCCEE
LRVFIGKRVASVSANPGFDLQALAERAVAMAKVSPEDPFACLADEASLAKSYPDLQLLDT
EEEEECCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHCCCCEEEEEH
TEVSSEMLREAGLAAEAAALAVKGVTNSSGAGASAGMGGLVLATSHGFEGSYMASRFGHS
HHHHHHHHHHCCCHHHHHHEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCE
VSVIAGEGTGMERDYDFDSRLYYAELDDPADIGRRAGERVIKRINPRQVPTGKDVTVIFD
EEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
PRVARGFVGHIAGAINGAAVARKSSFLRDRMGQQVLKSGLSITDDPLIVRGPSSRPFDGE
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
GVSGERMVMIEDGVLKHWFLSTSTGRELGLQTNGRGVRGGTAVSPSSTNLALEPGDITPE
CCCCCEEEEEECCCEEEEEEECCCCCEECCCCCCCCCCCCCEECCCCCEEEEECCCCCHH
ELIRSVGNGFYVTELIGHGVNMITGEYSRGATGFWIENGELTFPVSEVTIASNLKDMFMR
HHHHHHCCCEEEEHHHHCCCEEEECCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHHHC
LTPANDIDRKFGVAAPTFAIEGMTLAGR
CCCCCCHHHHHCCCCCCEEECCEEECCC
>Mature Secondary Structure 
SSEIDSSTLLSRASQLIDLARKAGADAADAVVVRSRSQSVSVRLGKVEGTESSESDDFS
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCEEEEEEEEECCCCCCCCCCEE
LRVFIGKRVASVSANPGFDLQALAERAVAMAKVSPEDPFACLADEASLAKSYPDLQLLDT
EEEEECCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHCCCCEEEEEH
TEVSSEMLREAGLAAEAAALAVKGVTNSSGAGASAGMGGLVLATSHGFEGSYMASRFGHS
HHHHHHHHHHCCCHHHHHHEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCE
VSVIAGEGTGMERDYDFDSRLYYAELDDPADIGRRAGERVIKRINPRQVPTGKDVTVIFD
EEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
PRVARGFVGHIAGAINGAAVARKSSFLRDRMGQQVLKSGLSITDDPLIVRGPSSRPFDGE
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
GVSGERMVMIEDGVLKHWFLSTSTGRELGLQTNGRGVRGGTAVSPSSTNLALEPGDITPE
CCCCCEEEEEECCCEEEEEEECCCCCEECCCCCCCCCCCCCEECCCCCEEEEECCCCCHH
ELIRSVGNGFYVTELIGHGVNMITGEYSRGATGFWIENGELTFPVSEVTIASNLKDMFMR
HHHHHHCCCEEEEHHHHCCCEEEECCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHHHC
LTPANDIDRKFGVAAPTFAIEGMTLAGR
CCCCCCHHHHHCCCCCCEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]