Click here to switch to the map view.

The map label for this gene is rlmE [H]

Identifier: 209548035

GI number: 209548035

Start: 439166

End: 439885

Strand: Direct

Name: rlmE [H]

Synonym: Rleg2_0428

Alternate gene names: 209548035

Gene position: 439166-439885 (Clockwise)

Preceding gene: 209548034

Following gene: 209548036

Centisome position: 9.68

GC content: 62.78

Gene sequence:

>720_bases
ATGACCAAGGCACCGATCGCGGGAAACCGCACCGGCCGCAAGCTCGGCCAGCGCGTCAAGAACAAGAAGATGAAGGCCTC
CTCTCGCCAATGGCTGCAGCGCCACATCAACGATCCCTATGTGCAGCGCGCCCAGCTGGAAGGCTATCGCGCCCGCGCCG
CCTTCAAGCTTTTGGAGATCGACGAGAAGCACCATATCCTGCGCGGCGCCAAGCGCATTATCGATCTGGGTGCCGCCCCC
GGCAGCTGGTCGCAGATCGCCGCCAAGGTCACCGGCTCGACGGACGAGGATATCCGTGTGGCCGCGATCGATTTTCTCGA
AATGGCCCAGCTGCCCGGCGTCAAGATCCTGCAGCTCGATTTCCTCGATCCGACTGCGCCGGAAAAGCTGCTGGAAGCGG
TCGGCGGCACGCCCGATCTTGTCATCTCCGATATGGCGGCGCCAACCACCGGCCACCATCGCACCGACCATCTGCGCACC
ATGCATCTCTGCGAAGTCGCGGCGCAATTTGCCGTCGAGGTGTTGGGGGAGGGCGGACATTTCCTCACCAAGACCTTCCA
GGGCGGCACCGAGCGTGAGCTGCTCGCCATGCTGAAGCAGAACTTCCGCCAGGTCGTCCATGTCAAGCCCAATGCCTCAC
GCGCCGAATCGGTCGAGATGTTTTTGCTCGCCAAGGGCTTCAAGGGGCGCAAGGTCGAAGGCGACGCTGAGAAAGCTTGA

Upstream 100 bases:

>100_bases
CGGCTTGAGGGAAGGCCTGCTCACCGACATGATGGCCGATGACGGCGTGTGGCGGCGCAACCGCAACCGCCGCGGCCAGC
GAGCCAGATAAGGAAAAGGC

Downstream 100 bases:

>100_bases
TCTTGGGCCGGTCCGGGCCGATGATGACGGTCCAAAGAGCTGAACTGCTGATCCCATGCTTCTTTACATCACGCTCGGAA
CCAACGACCTTTATCGCGCA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTKAPIAGNRTGRKLGQRVKNKKMKASSRQWLQRHINDPYVQRAQLEGYRARAAFKLLEIDEKHHILRGAKRIIDLGAAP
GSWSQIAAKVTGSTDEDIRVAAIDFLEMAQLPGVKILQLDFLDPTAPEKLLEAVGGTPDLVISDMAAPTTGHHRTDHLRT
MHLCEVAAQFAVEVLGEGGHFLTKTFQGGTERELLAMLKQNFRQVVHVKPNASRAESVEMFLLAKGFKGRKVEGDAEKA

Sequences:

>Translated_239_residues
MTKAPIAGNRTGRKLGQRVKNKKMKASSRQWLQRHINDPYVQRAQLEGYRARAAFKLLEIDEKHHILRGAKRIIDLGAAP
GSWSQIAAKVTGSTDEDIRVAAIDFLEMAQLPGVKILQLDFLDPTAPEKLLEAVGGTPDLVISDMAAPTTGHHRTDHLRT
MHLCEVAAQFAVEVLGEGGHFLTKTFQGGTERELLAMLKQNFRQVVHVKPNASRAESVEMFLLAKGFKGRKVEGDAEKA
>Mature_238_residues
TKAPIAGNRTGRKLGQRVKNKKMKASSRQWLQRHINDPYVQRAQLEGYRARAAFKLLEIDEKHHILRGAKRIIDLGAAPG
SWSQIAAKVTGSTDEDIRVAAIDFLEMAQLPGVKILQLDFLDPTAPEKLLEAVGGTPDLVISDMAAPTTGHHRTDHLRTM
HLCEVAAQFAVEVLGEGGHFLTKTFQGGTERELLAMLKQNFRQVVHVKPNASRAESVEMFLLAKGFKGRKVEGDAEKA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=224, Percent_Identity=39.7321428571429, Blast_Score=165, Evalue=3e-41,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=37.17277486911, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=37.17277486911, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=37.17277486911, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI194097365, Length=192, Percent_Identity=34.8958333333333, Blast_Score=111, Evalue=6e-25,
Organism=Escherichia coli, GI1789569, Length=209, Percent_Identity=41.6267942583732, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=38.974358974359, Blast_Score=134, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17553474, Length=213, Percent_Identity=35.6807511737089, Blast_Score=126, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=29.5336787564767, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=29.5336787564767, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=29.5336787564767, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=35.678391959799, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6319796, Length=197, Percent_Identity=36.0406091370558, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=108, Percent_Identity=35.1851851851852, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356387, Length=226, Percent_Identity=37.1681415929204, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=37.2448979591837, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=36.3636363636364, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859957, Length=192, Percent_Identity=32.2916666666667, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 26407; Mature: 26276

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAPIAGNRTGRKLGQRVKNKKMKASSRQWLQRHINDPYVQRAQLEGYRARAAFKLLEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHH
DEKHHILRGAKRIIDLGAAPGSWSQIAAKVTGSTDEDIRVAAIDFLEMAQLPGVKILQLD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCEEEEEE
FLDPTAPEKLLEAVGGTPDLVISDMAAPTTGHHRTDHLRTMHLCEVAAQFAVEVLGEGGH
CCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FLTKTFQGGTERELLAMLKQNFRQVVHVKPNASRAESVEMFLLAKGFKGRKVEGDAEKA
EEHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKAPIAGNRTGRKLGQRVKNKKMKASSRQWLQRHINDPYVQRAQLEGYRARAAFKLLEI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHH
DEKHHILRGAKRIIDLGAAPGSWSQIAAKVTGSTDEDIRVAAIDFLEMAQLPGVKILQLD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCEEEEEE
FLDPTAPEKLLEAVGGTPDLVISDMAAPTTGHHRTDHLRTMHLCEVAAQFAVEVLGEGGH
CCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FLTKTFQGGTERELLAMLKQNFRQVVHVKPNASRAESVEMFLLAKGFKGRKVEGDAEKA
EEHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA