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The map label for this gene is rrmA [C]

Identifier: 209548030

GI number: 209548030

Start: 432661

End: 433473

Strand: Direct

Name: rrmA [C]

Synonym: Rleg2_0422

Alternate gene names: 209548030

Gene position: 432661-433473 (Clockwise)

Preceding gene: 209548029

Following gene: 209548031

Centisome position: 9.53

GC content: 55.35

Gene sequence:

>813_bases
ATGTCATTTGTTCGTACGACTCTCAACCGGTTTCCGGTGTTGAAGAGCCAGCTGAAGCGATTGCGTGAACGGCTGCAGCC
CGCGGCATCGGTATCGTCCCACTACGTCGAGATCGATTCAGCTCGGCGAGACAGCGAAAGCTCGCGTCTTGCCGCATCAT
GGAAAGCGAATGATCTGCCTGCGCGCCAGAGAGCGCTCGTCGAGCGCCAGTTGAAGGAATATCGCGGCGGCGCTTCCATC
GATGTATTCGATGTTTTTACCGCTGCGCTGCGTAAAATCGACAATCTCCCGGCCGCCGGTTCGTTGCTCGAAATCGGTTG
CTCCAGCGGATATTACTCCGAGGTCCTGGAGGTAAATGGCCTGCCGTTGCGGTATCACGGCTGCGATTATTCCGATGCCT
TCATCGACATGGCGCGCCGCATCTATCCGGCACTGTCGTTCGATGTGGAGGATGCAACACGGCTCAGTTATCAGGACAAT
GCTTTTGATATTGTGATTTCGGGATGCTGCCTGCTGCATATCGCCGACTATGAGGCGGCGATTGCAGAAAGCGCCCGCGT
CGCCAAAGACTACGTCATCTTTCACCGCACTCCCATCGTCTATGGCGAACCGACCAAGTATTTCCGCAAGCAGGCCTATG
GCGTCGAGACAATGGAGATCCATTTCTCCGAACCGCAGCTATTGGACAGCTTTCGGGTTCACGGCCTCGAAGTCCTTTCG
ACCTTCACGCTGAATGAAAACGCGGATCCGCGTGACAGCAGCAAGGGCAATGCAAACCGCACTTATTTGTGCAGGAAACA
ATCCCAGTCATGA

Upstream 100 bases:

>100_bases
CGAAGGCCGGCCAGGCGAGGACGCTGAAAGATCACACCTACAAGTCGAGAATGAAAGAGTTGATGCCGATCCTCGAGAGA
CATCTGGAGAAACAGGGCTG

Downstream 100 bases:

>100_bases
CGACCTACTTCTGTACCCTTTTCGACAGCGGTTATCTCATCAAGGGGATGGCCATGATGGAGAGCCTCGTTCGATGGTGC
CCAGACGCGCATATTTTTGT

Product: type 11 methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: Radical SAM Domain Protein

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSFVRTTLNRFPVLKSQLKRLRERLQPAASVSSHYVEIDSARRDSESSRLAASWKANDLPARQRALVERQLKEYRGGASI
DVFDVFTAALRKIDNLPAAGSLLEIGCSSGYYSEVLEVNGLPLRYHGCDYSDAFIDMARRIYPALSFDVEDATRLSYQDN
AFDIVISGCCLLHIADYEAAIAESARVAKDYVIFHRTPIVYGEPTKYFRKQAYGVETMEIHFSEPQLLDSFRVHGLEVLS
TFTLNENADPRDSSKGNANRTYLCRKQSQS

Sequences:

>Translated_270_residues
MSFVRTTLNRFPVLKSQLKRLRERLQPAASVSSHYVEIDSARRDSESSRLAASWKANDLPARQRALVERQLKEYRGGASI
DVFDVFTAALRKIDNLPAAGSLLEIGCSSGYYSEVLEVNGLPLRYHGCDYSDAFIDMARRIYPALSFDVEDATRLSYQDN
AFDIVISGCCLLHIADYEAAIAESARVAKDYVIFHRTPIVYGEPTKYFRKQAYGVETMEIHFSEPQLLDSFRVHGLEVLS
TFTLNENADPRDSSKGNANRTYLCRKQSQS
>Mature_269_residues
SFVRTTLNRFPVLKSQLKRLRERLQPAASVSSHYVEIDSARRDSESSRLAASWKANDLPARQRALVERQLKEYRGGASID
VFDVFTAALRKIDNLPAAGSLLEIGCSSGYYSEVLEVNGLPLRYHGCDYSDAFIDMARRIYPALSFDVEDATRLSYQDNA
FDIVISGCCLLHIADYEAAIAESARVAKDYVIFHRTPIVYGEPTKYFRKQAYGVETMEIHFSEPQLLDSFRVHGLEVLST
FTLNENADPRDSSKGNANRTYLCRKQSQS

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 30500; Mature: 30368

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVRTTLNRFPVLKSQLKRLRERLQPAASVSSHYVEIDSARRDSESSRLAASWKANDLP
CCHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHEEEECCCCCCCCCHHEEECCCCCCCC
ARQRALVERQLKEYRGGASIDVFDVFTAALRKIDNLPAAGSLLEIGCSSGYYSEVLEVNG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHCCC
LPLRYHGCDYSDAFIDMARRIYPALSFDVEDATRLSYQDNAFDIVISGCCLLHIADYEAA
CEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHCCCEEEECCHHHH
IAESARVAKDYVIFHRTPIVYGEPTKYFRKQAYGVETMEIHFSEPQLLDSFRVHGLEVLS
HHHHHHHHHHEEEEECCCEEECCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
TFTLNENADPRDSSKGNANRTYLCRKQSQS
EEEECCCCCCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
SFVRTTLNRFPVLKSQLKRLRERLQPAASVSSHYVEIDSARRDSESSRLAASWKANDLP
CHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHEEEECCCCCCCCCHHEEECCCCCCCC
ARQRALVERQLKEYRGGASIDVFDVFTAALRKIDNLPAAGSLLEIGCSSGYYSEVLEVNG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHCCC
LPLRYHGCDYSDAFIDMARRIYPALSFDVEDATRLSYQDNAFDIVISGCCLLHIADYEAA
CEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHCCCEEEECCHHHH
IAESARVAKDYVIFHRTPIVYGEPTKYFRKQAYGVETMEIHFSEPQLLDSFRVHGLEVLS
HHHHHHHHHHEEEEECCCEEECCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
TFTLNENADPRDSSKGNANRTYLCRKQSQS
EEEECCCCCCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA