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The map label for this gene is dadA [H]

Identifier: 209548004

GI number: 209548004

Start: 409494

End: 410741

Strand: Reverse

Name: dadA [H]

Synonym: Rleg2_0396

Alternate gene names: 209548004

Gene position: 410741-409494 (Counterclockwise)

Preceding gene: 209548005

Following gene: 209547993

Centisome position: 9.05

GC content: 64.5

Gene sequence:

>1248_bases
ATGACGAAAAACGCAATCGTGCTCGGCGCCGGCATCGTCGGCGTGTCGACGGCTATCCATCTTCAACGGCGCGGCCGGCA
GGTGACGCTGATCGACCGCAAGGATCCCGGCAACGAAACCTCCTTCGGCAATGCCGGGCTGATCCAGCGCGAAGGTGTTG
CGCCCTACGGCTTTCCGCAGCAGCTCGGCCTGTTGCTGCGTTATGCGCTGAACAACCGCATCGATGCGCATTACCATCTG
CGCACGCTGCCGAGCCAGCTTGCCTTTCTCGCACGCTACTGGTGGAATTCGAACGCCCGGCGCCACGCGATCATCACCCG
GGCCTATGCGCCACTGATCGAAAATTCGATTACCGAGCACAAGGACCTGATCGAAGCGTCCGAGGCGCAAGCGCTGATCC
GCAGGGATGGCTGGATAAAGATTTTCCGCACGCCTGCCAGGCGTGACGAAGCCCTTGGTGAGGCAGCACTTTGGCGGAAC
GAATTCGGGGTGGCATATGACAGCCTGACCTCGGCCGATATCGCCCGCATGGAGCCTGACATGACAGGCGATTTTGCCGG
TGGCATCCGCTGGCGTGATCCCTGGTCGGTGCTCGATCCGCATGCGCTGACATCAGCCTATCGCGGCTATTTCGAAAGCC
TCGGCGGCAGGTTCGTCACAGGCGACGCCGCCTCGCTAGGCCAGTTCGGCTCCGGTTGGAAGATCATGACCGGGGAAGGC
CCGCTCGAAGCCGACGATGCGGTGATCGCACTCGGCCCCTGGGCAGCGCTCGCGACGCGCCGCCTCGGCTATTCCTTGTC
CCTCGGCGTCAAGCGCGGCTATCACATGCATTATGCCGCCAAGGGCAATGCGGTGCTCAACAACTGGATGCTCGATGCCG
AACGCGGCTATCTCCTGGCGCCGATGCGCCGCGGCATCCGGCTGACGACGGGCGCGGAGTTCGCAACGGTTGATGCGCCG
AAGACACCGGTCCAGCTCGACCGGGCCGAGGCGGTGGCCCGTACGATCTTCCCGCTCGGCGACAGGCTCGATCCCGAACC
CTGGATGGGCGCCCGCCCCTGCACGCCGGACATGATGCCGATCATCGGCAAAGCGCCGCGCCATCAGGGCCTGTGGTTCG
CCTTCGGACATGCCCATCACGGGCTGACGCTCGGGCCGGTGACCGGCCGGGTGCTGGCCGAACTCATCACCGGCGAGACG
CCGTTCATCGATATTGCGGCTTATGCGCCGCAGCGTTTCAACCCGTGA

Upstream 100 bases:

>100_bases
ACTGAAAAGCGCAATGATTTTGAAATGAAGGCGATTTTTCGTTTGCCCCGCGGTATCGGCAGGGGCACTTTCACTGCGAT
AACAATGCGGAATGCAGGGA

Downstream 100 bases:

>100_bases
CGCCGAGCGTGGCAAGCCCGTCGAGGGCCGTTGCCGCGATGCCTTGCACCGTATCGTCAATATCGACGGTGACGACACCT
TGCTCGCCTGTCGGCACCTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTKNAIVLGAGIVGVSTAIHLQRRGRQVTLIDRKDPGNETSFGNAGLIQREGVAPYGFPQQLGLLLRYALNNRIDAHYHL
RTLPSQLAFLARYWWNSNARRHAIITRAYAPLIENSITEHKDLIEASEAQALIRRDGWIKIFRTPARRDEALGEAALWRN
EFGVAYDSLTSADIARMEPDMTGDFAGGIRWRDPWSVLDPHALTSAYRGYFESLGGRFVTGDAASLGQFGSGWKIMTGEG
PLEADDAVIALGPWAALATRRLGYSLSLGVKRGYHMHYAAKGNAVLNNWMLDAERGYLLAPMRRGIRLTTGAEFATVDAP
KTPVQLDRAEAVARTIFPLGDRLDPEPWMGARPCTPDMMPIIGKAPRHQGLWFAFGHAHHGLTLGPVTGRVLAELITGET
PFIDIAAYAPQRFNP

Sequences:

>Translated_415_residues
MTKNAIVLGAGIVGVSTAIHLQRRGRQVTLIDRKDPGNETSFGNAGLIQREGVAPYGFPQQLGLLLRYALNNRIDAHYHL
RTLPSQLAFLARYWWNSNARRHAIITRAYAPLIENSITEHKDLIEASEAQALIRRDGWIKIFRTPARRDEALGEAALWRN
EFGVAYDSLTSADIARMEPDMTGDFAGGIRWRDPWSVLDPHALTSAYRGYFESLGGRFVTGDAASLGQFGSGWKIMTGEG
PLEADDAVIALGPWAALATRRLGYSLSLGVKRGYHMHYAAKGNAVLNNWMLDAERGYLLAPMRRGIRLTTGAEFATVDAP
KTPVQLDRAEAVARTIFPLGDRLDPEPWMGARPCTPDMMPIIGKAPRHQGLWFAFGHAHHGLTLGPVTGRVLAELITGET
PFIDIAAYAPQRFNP
>Mature_414_residues
TKNAIVLGAGIVGVSTAIHLQRRGRQVTLIDRKDPGNETSFGNAGLIQREGVAPYGFPQQLGLLLRYALNNRIDAHYHLR
TLPSQLAFLARYWWNSNARRHAIITRAYAPLIENSITEHKDLIEASEAQALIRRDGWIKIFRTPARRDEALGEAALWRNE
FGVAYDSLTSADIARMEPDMTGDFAGGIRWRDPWSVLDPHALTSAYRGYFESLGGRFVTGDAASLGQFGSGWKIMTGEGP
LEADDAVIALGPWAALATRRLGYSLSLGVKRGYHMHYAAKGNAVLNNWMLDAERGYLLAPMRRGIRLTTGAEFATVDAPK
TPVQLDRAEAVARTIFPLGDRLDPEPWMGARPCTPDMMPIIGKAPRHQGLWFAFGHAHHGLTLGPVTGRVLAELITGETP
FIDIAAYAPQRFNP

Specific function: Oxidative deamination of D-amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=427, Percent_Identity=25.0585480093677, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076
- InterPro:   IPR006036 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45759; Mature: 45628

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNAIVLGAGIVGVSTAIHLQRRGRQVTLIDRKDPGNETSFGNAGLIQREGVAPYGFPQ
CCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCHH
QLGLLLRYALNNRIDAHYHLRTLPSQLAFLARYWWNSNARRHAIITRAYAPLIENSITEH
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHH
KDLIEASEAQALIRRDGWIKIFRTPARRDEALGEAALWRNEFGVAYDSLTSADIARMEPD
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHCCCC
MTGDFAGGIRWRDPWSVLDPHALTSAYRGYFESLGGRFVTGDAASLGQFGSGWKIMTGEG
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCHHHCCCCCCCEEEECCC
PLEADDAVIALGPWAALATRRLGYSLSLGVKRGYHMHYAAKGNAVLNNWMLDAERGYLLA
CCCCCCEEEEECCHHHHHHHHCCCEEEECCCCCEEEEEECCCCEEEECEEEECCCCEEEE
PMRRGIRLTTGAEFATVDAPKTPVQLDRAEAVARTIFPLGDRLDPEPWMGARPCTPDMMP
HHHCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
IIGKAPRHQGLWFAFGHAHHGLTLGPVTGRVLAELITGETPFIDIAAYAPQRFNP
HHCCCCCCCCCEEEECCCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TKNAIVLGAGIVGVSTAIHLQRRGRQVTLIDRKDPGNETSFGNAGLIQREGVAPYGFPQ
CCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCHH
QLGLLLRYALNNRIDAHYHLRTLPSQLAFLARYWWNSNARRHAIITRAYAPLIENSITEH
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHH
KDLIEASEAQALIRRDGWIKIFRTPARRDEALGEAALWRNEFGVAYDSLTSADIARMEPD
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHCCCC
MTGDFAGGIRWRDPWSVLDPHALTSAYRGYFESLGGRFVTGDAASLGQFGSGWKIMTGEG
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCHHHCCCCCCCEEEECCC
PLEADDAVIALGPWAALATRRLGYSLSLGVKRGYHMHYAAKGNAVLNNWMLDAERGYLLA
CCCCCCEEEEECCHHHHHHHHCCCEEEECCCCCEEEEEECCCCEEEECEEEECCCCEEEE
PMRRGIRLTTGAEFATVDAPKTPVQLDRAEAVARTIFPLGDRLDPEPWMGARPCTPDMMP
HHHCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
IIGKAPRHQGLWFAFGHAHHGLTLGPVTGRVLAELITGETPFIDIAAYAPQRFNP
HHCCCCCCCCCEEEECCCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]