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The map label for this gene is yafC [H]

Identifier: 209547992

GI number: 209547992

Start: 397229

End: 398110

Strand: Reverse

Name: yafC [H]

Synonym: Rleg2_0384

Alternate gene names: 209547992

Gene position: 398110-397229 (Counterclockwise)

Preceding gene: 209547993

Following gene: 209547989

Centisome position: 8.77

GC content: 63.15

Gene sequence:

>882_bases
ATGAACTGGGACGACGTCCGGATTTTCCTTGCCGTCGCCCGCACCGGGCAGATCCTTGCCGCCTCGAAGCGGCTGGGGCT
CAATCACGCTACGCTCTCTCGCCGGCTTACCTCGCTGGAAGAGGCGCTGAAGACGCGGCTTTTCATTCGCCGGACCAATG
GCTGCGAGCTGACGGCGGAAGGCGAAGTGTTCTTGGCATCGGCCGAGCGGATGGAAACGGAAATGCTCGCTGCACAGGCG
AGCCTCGGCCACACCGATACGGCGATCGCCGGGACGGTGCGCGTCGGCGCGCCTGACGGCTTCGGCGTTTCTTTTCTGGC
GCCGCGCATGGGCCGGCTGATTGAGCGTCATCCGGCACTGAAGATCCAGCTCGTCCCGGTGCCGCGCTCTTTTTCGCTGT
CGCAGCGCGAGGCCGATATCGCCATTACGCTGGAGCGGCCCGAACAGGGCCGGCTCGTCTCCTCCAAGCTCACAGACTAC
ACGCTCGGCCTCTATGCCTCGCGTGACTATCTCGCCTCCCGGGGCACGCCAAGCGATATCGAGGCGCTGAAGGCGCATCC
ACGCATCGGCTATGTCGAAGACCTGATCTTCACCGCGTCGCTGAATTTCTCCGGCGAGGTGATGCGAAGCTGGGATGCAA
GCTTCGAGATTTCGACGGCGATCGGGCAGACCGAGGCTGTGCGCTCGGGCGCCGGCATCTGTATTCTGCACGATTATATT
GCCCGGCAATATCCGGAGCTCCAGCGCATCCTGCCTGATATTTCGATCCGGCGCGCCTACTGGACGACCTATCACGAAAC
GGCGCGCGACCTGGTGCGCGTCAGGAGCGTTGTCGATTTCCTGCAGGAACTCATCGGCGCCGAGCGGCAGATCTTTCTTT
AG

Upstream 100 bases:

>100_bases
TGCGGACACCGCATTTCCTCAAATACAAAGTCGAGACGGCCGGCATGGTGACATCGCTGACGCTTCTCGACGTCGATCCG
ATCGCAATGCGGGCCAAGCC

Downstream 100 bases:

>100_bases
AGCCAACGTGCCTTGCCGGCTCGTCATTCTCATCCGGATTGGGAACCGGCGCCTCGTCAGGCAGCCTGTCCGGCTCGGGC
TCCGTGATCGGCGGCACCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNWDDVRIFLAVARTGQILAASKRLGLNHATLSRRLTSLEEALKTRLFIRRTNGCELTAEGEVFLASAERMETEMLAAQA
SLGHTDTAIAGTVRVGAPDGFGVSFLAPRMGRLIERHPALKIQLVPVPRSFSLSQREADIAITLERPEQGRLVSSKLTDY
TLGLYASRDYLASRGTPSDIEALKAHPRIGYVEDLIFTASLNFSGEVMRSWDASFEISTAIGQTEAVRSGAGICILHDYI
ARQYPELQRILPDISIRRAYWTTYHETARDLVRVRSVVDFLQELIGAERQIFL

Sequences:

>Translated_293_residues
MNWDDVRIFLAVARTGQILAASKRLGLNHATLSRRLTSLEEALKTRLFIRRTNGCELTAEGEVFLASAERMETEMLAAQA
SLGHTDTAIAGTVRVGAPDGFGVSFLAPRMGRLIERHPALKIQLVPVPRSFSLSQREADIAITLERPEQGRLVSSKLTDY
TLGLYASRDYLASRGTPSDIEALKAHPRIGYVEDLIFTASLNFSGEVMRSWDASFEISTAIGQTEAVRSGAGICILHDYI
ARQYPELQRILPDISIRRAYWTTYHETARDLVRVRSVVDFLQELIGAERQIFL
>Mature_293_residues
MNWDDVRIFLAVARTGQILAASKRLGLNHATLSRRLTSLEEALKTRLFIRRTNGCELTAEGEVFLASAERMETEMLAAQA
SLGHTDTAIAGTVRVGAPDGFGVSFLAPRMGRLIERHPALKIQLVPVPRSFSLSQREADIAITLERPEQGRLVSSKLTDY
TLGLYASRDYLASRGTPSDIEALKAHPRIGYVEDLIFTASLNFSGEVMRSWDASFEISTAIGQTEAVRSGAGICILHDYI
ARQYPELQRILPDISIRRAYWTTYHETARDLVRVRSVVDFLQELIGAERQIFL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=28.6689419795222, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1789440, Length=283, Percent_Identity=24.0282685512367, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=166, Percent_Identity=28.3132530120482, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI145693193, Length=191, Percent_Identity=26.7015706806283, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789639, Length=187, Percent_Identity=24.5989304812834, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994962, Length=173, Percent_Identity=30.635838150289, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=23.7931034482759, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32651; Mature: 32651

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWDDVRIFLAVARTGQILAASKRLGLNHATLSRRLTSLEEALKTRLFIRRTNGCELTAE
CCCCCEEEEEEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEC
GEVFLASAERMETEMLAAQASLGHTDTAIAGTVRVGAPDGFGVSFLAPRMGRLIERHPAL
CCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCE
KIQLVPVPRSFSLSQREADIAITLERPEQGRLVSSKLTDYTLGLYASRDYLASRGTPSDI
EEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCHHEEEEEECCHHHHCCCCCHHH
EALKAHPRIGYVEDLIFTASLNFSGEVMRSWDASFEISTAIGQTEAVRSGAGICILHDYI
HHHHCCCCCCHHHHEEEEEECCCCCHHHHHCCCCEEEEHHHCCHHHHHCCCCEEEEEHHH
ARQYPELQRILPDISIRRAYWTTYHETARDLVRVRSVVDFLQELIGAERQIFL
HHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MNWDDVRIFLAVARTGQILAASKRLGLNHATLSRRLTSLEEALKTRLFIRRTNGCELTAE
CCCCCEEEEEEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEC
GEVFLASAERMETEMLAAQASLGHTDTAIAGTVRVGAPDGFGVSFLAPRMGRLIERHPAL
CCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCE
KIQLVPVPRSFSLSQREADIAITLERPEQGRLVSSKLTDYTLGLYASRDYLASRGTPSDI
EEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCHHEEEEEECCHHHHCCCCCHHH
EALKAHPRIGYVEDLIFTASLNFSGEVMRSWDASFEISTAIGQTEAVRSGAGICILHDYI
HHHHCCCCCCHHHHEEEEEECCCCCHHHHHCCCCEEEEHHHCCHHHHHCCCCEEEEEHHH
ARQYPELQRILPDISIRRAYWTTYHETARDLVRVRSVVDFLQELIGAERQIFL
HHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]