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The map label for this gene is acsA1 [H]

Identifier: 209547988

GI number: 209547988

Start: 389521

End: 391473

Strand: Direct

Name: acsA1 [H]

Synonym: Rleg2_0380

Alternate gene names: 209547988

Gene position: 389521-391473 (Clockwise)

Preceding gene: 209547986

Following gene: 209547990

Centisome position: 8.58

GC content: 63.9

Gene sequence:

>1953_bases
ATGCAGGATAACAGACCACTTTGGGTGCCCTCGGCGGAAGCCGTCGCAAGAAGCCCGATCCACGCCTTCATGGCGCGCTG
CAATGCCGATTTCGGGCTTTCGCTTTCGGGCTTTGAAGATCTGCATGCCTGGTCGGTGGCCGAGCGGGAGAATTTCTGGT
CGGCCGTCTGGGATTTCTGCGGCGTGAAGGGGGAGCGCGGCGCAGAGGTGCTCATTAATGGCGACCGCATGCTGGAGGCT
CGCTTCTTTCCAAATGCGACGCTGAACTTCGCTGAGAACCTGCTGGCCGGCCGCGGTGATGGTGATGCCATCGTCTTTCG
CGGCGAGGACAAGGCTGAGGATCGCTGGTCGTGGGACCGGCTGCGGGCGCTGGTCTCGAAGCTGCAGCAGGCCTTCAAGG
CGCAGGGGATCGGCGAGGGCGACCGTATTGCTGCGATGATGCCGAACATGCCGGAGACCGTCGCCGCCATGCTGGCGGCC
GCCTCGATCGGCGCTATCTGGTCGTCCTGCTCCCCCGATTTCGGCGAGCAGGGCGTGCTCGACCGCTTCGGCCAGATCGG
CCCGAAGCTGTTCATCGCCTGCGATGCCTATTGGTATTCCGGCAAGCTGCAGGATGTCGGCCCCAAGGTCGCCGCGGTCG
CCAAAAGCTTGGGCGTGCCGACGATCGTCGTCCACTATGCCGGCGATGCCGAGGCGGTGGCGCGCGCAACGCCTGGGGCT
TCGACGCTGGAAACCTTCGTCGCGCCCTATCAGGCAAGAGAGGTCGAGTTCACGCCGCTTGCCTTTGCCCATCCACTCTA
CATTCTGTTTTCCTCCGGCACCACGGGCGTGCCGAAATGCATCGTCCATTCGGCCGGCGGCACGCTGTTGCAGCATCTCA
AGGAGCAGCGGCTGCATTGCGGCCTTAAGGCCGGCGAGAAGCTCTTCTACTTCACCACCTGCGGCTGGATGATGTGGAAC
TGGCTGGTCAGCGGTCTTGCCAGCGGCGCCACGCTCTGCCTGTTCGACGGTTCGCCTTTCGCGCCGGATGGCAATGTGCT
GTTCGACTACGCCGCGGCCGAGAACTTCGCGATCTTCGGCACCTCTGCGAAATATATCGATGCGGTGCGCAAAAGCGGGC
TGACGCCGCGCACGAGCCACGATCTTTCCAGCCTGCGGCTTATGACCTCGACCGGTTCGCCGCTGTCGCCTGAGGGCTTC
ACCTTCGTCTACGAGGGCATCAAGGCGGATGTGCAGCTCGCCTCGATCTCCGGCGGCACCGATATCGTCTCCTGCTTCGT
GCTCGGCAATCCGCTGCAGCCGGTCTGGCGCGGCGAGATCCAGGGGGCCGGCCTCGGCCTTGCGGTCGATGTCTGGAACG
ATGACGGCAAGCCGGTGCGCGGCGAAAAGGGTGAACTCGTCTGCACCAAGGCCTTCCCGTCGATGCCGGTGATGTTCTGG
AACGATCCCGACGGCGCCAAATATCGCGCCGCCTATTTCGACCGGTTCGACAATGTCTGGTGCCATGGCGATTTTGCCGA
ATGGACCGAGCATGGCGGCCTCGTCATCCACGGCCGTTCGGATGCGACGCTCAATCCCGGCGGCGTGCGCATCGGCACGG
CCGAGATTTATAATCAGGTGGAGCAAATGGAGGAAGTGGCCGAGGCGCTGTGCATCGGCCAGGAGTGGGACGACGACGTG
CGCGTCATCCTCTTCGTTCGCCTTGCCCCCGGCGTGACGCTGACCGAGGATCTGATCAAGGCGATCAAGACGCGGATCCG
CACCGGCGCCTCGCCGCGGCACGTGCCGGCCAAGATCATCGCGGTCGCCGATATCCCCCGCACCAAATCCGGCAAGATCG
TCGAGCTTGCGGTGCGCGAGGTCGTTCACAACAGGCCGGTGAAAAACCAGGAAGCGCTCGCCAATCCCGAGGCGTTGCGG
CTCTTTGCCGGGCTGGAGGAACTCAAGGATTGA

Upstream 100 bases:

>100_bases
ACGCATCGGCCTTCTGAATGCATAGGTTGGGATCGCGTCGGTTCCGCACTTTATAATGCAATGCAGCATGATATAAAGGC
GGGATTGGGAGGAGGATTGA

Downstream 100 bases:

>100_bases
GAGCGGACCGGAAGCTACAAGCTTTTATCACTTATCGATGCACTGTCATGGAAACCTTTCGTTAAGAAAGGTTTGTCAAA
TCTAAAAGCCAACTTGGGGG

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MQDNRPLWVPSAEAVARSPIHAFMARCNADFGLSLSGFEDLHAWSVAERENFWSAVWDFCGVKGERGAEVLINGDRMLEA
RFFPNATLNFAENLLAGRGDGDAIVFRGEDKAEDRWSWDRLRALVSKLQQAFKAQGIGEGDRIAAMMPNMPETVAAMLAA
ASIGAIWSSCSPDFGEQGVLDRFGQIGPKLFIACDAYWYSGKLQDVGPKVAAVAKSLGVPTIVVHYAGDAEAVARATPGA
STLETFVAPYQAREVEFTPLAFAHPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEQRLHCGLKAGEKLFYFTTCGWMMWN
WLVSGLASGATLCLFDGSPFAPDGNVLFDYAAAENFAIFGTSAKYIDAVRKSGLTPRTSHDLSSLRLMTSTGSPLSPEGF
TFVYEGIKADVQLASISGGTDIVSCFVLGNPLQPVWRGEIQGAGLGLAVDVWNDDGKPVRGEKGELVCTKAFPSMPVMFW
NDPDGAKYRAAYFDRFDNVWCHGDFAEWTEHGGLVIHGRSDATLNPGGVRIGTAEIYNQVEQMEEVAEALCIGQEWDDDV
RVILFVRLAPGVTLTEDLIKAIKTRIRTGASPRHVPAKIIAVADIPRTKSGKIVELAVREVVHNRPVKNQEALANPEALR
LFAGLEELKD

Sequences:

>Translated_650_residues
MQDNRPLWVPSAEAVARSPIHAFMARCNADFGLSLSGFEDLHAWSVAERENFWSAVWDFCGVKGERGAEVLINGDRMLEA
RFFPNATLNFAENLLAGRGDGDAIVFRGEDKAEDRWSWDRLRALVSKLQQAFKAQGIGEGDRIAAMMPNMPETVAAMLAA
ASIGAIWSSCSPDFGEQGVLDRFGQIGPKLFIACDAYWYSGKLQDVGPKVAAVAKSLGVPTIVVHYAGDAEAVARATPGA
STLETFVAPYQAREVEFTPLAFAHPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEQRLHCGLKAGEKLFYFTTCGWMMWN
WLVSGLASGATLCLFDGSPFAPDGNVLFDYAAAENFAIFGTSAKYIDAVRKSGLTPRTSHDLSSLRLMTSTGSPLSPEGF
TFVYEGIKADVQLASISGGTDIVSCFVLGNPLQPVWRGEIQGAGLGLAVDVWNDDGKPVRGEKGELVCTKAFPSMPVMFW
NDPDGAKYRAAYFDRFDNVWCHGDFAEWTEHGGLVIHGRSDATLNPGGVRIGTAEIYNQVEQMEEVAEALCIGQEWDDDV
RVILFVRLAPGVTLTEDLIKAIKTRIRTGASPRHVPAKIIAVADIPRTKSGKIVELAVREVVHNRPVKNQEALANPEALR
LFAGLEELKD
>Mature_650_residues
MQDNRPLWVPSAEAVARSPIHAFMARCNADFGLSLSGFEDLHAWSVAERENFWSAVWDFCGVKGERGAEVLINGDRMLEA
RFFPNATLNFAENLLAGRGDGDAIVFRGEDKAEDRWSWDRLRALVSKLQQAFKAQGIGEGDRIAAMMPNMPETVAAMLAA
ASIGAIWSSCSPDFGEQGVLDRFGQIGPKLFIACDAYWYSGKLQDVGPKVAAVAKSLGVPTIVVHYAGDAEAVARATPGA
STLETFVAPYQAREVEFTPLAFAHPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEQRLHCGLKAGEKLFYFTTCGWMMWN
WLVSGLASGATLCLFDGSPFAPDGNVLFDYAAAENFAIFGTSAKYIDAVRKSGLTPRTSHDLSSLRLMTSTGSPLSPEGF
TFVYEGIKADVQLASISGGTDIVSCFVLGNPLQPVWRGEIQGAGLGLAVDVWNDDGKPVRGEKGELVCTKAFPSMPVMFW
NDPDGAKYRAAYFDRFDNVWCHGDFAEWTEHGGLVIHGRSDATLNPGGVRIGTAEIYNQVEQMEEVAEALCIGQEWDDDV
RVILFVRLAPGVTLTEDLIKAIKTRIRTGASPRHVPAKIIAVADIPRTKSGKIVELAVREVVHNRPVKNQEALANPEALR
LFAGLEELKD

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=658, Percent_Identity=43.0091185410334, Blast_Score=546, Evalue=1e-155,
Organism=Homo sapiens, GI8923896, Length=660, Percent_Identity=26.6666666666667, Blast_Score=178, Evalue=2e-44,
Organism=Homo sapiens, GI253970489, Length=673, Percent_Identity=25.7057949479941, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI13375727, Length=645, Percent_Identity=24.8062015503876, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI28416953, Length=588, Percent_Identity=23.8095238095238, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI38505220, Length=520, Percent_Identity=22.8846153846154, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1790505, Length=573, Percent_Identity=26.3525305410122, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1786529, Length=650, Percent_Identity=22.4615384615385, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17569507, Length=638, Percent_Identity=38.0877742946708, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71983001, Length=622, Percent_Identity=23.7942122186495, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71982997, Length=622, Percent_Identity=23.7942122186495, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323182, Length=669, Percent_Identity=25.2615844544096, Blast_Score=170, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6319264, Length=644, Percent_Identity=25.776397515528, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24667955, Length=607, Percent_Identity=26.5238879736409, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI62472339, Length=560, Percent_Identity=26.6071428571429, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24667959, Length=530, Percent_Identity=26.6037735849057, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21355225, Length=573, Percent_Identity=23.5602094240838, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 70640; Mature: 70640

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNRPLWVPSAEAVARSPIHAFMARCNADFGLSLSGFEDLHAWSVAERENFWSAVWDFC
CCCCCCEECCCHHHHHHCHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
GVKGERGAEVLINGDRMLEARFFPNATLNFAENLLAGRGDGDAIVFRGEDKAEDRWSWDR
CCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHH
LRALVSKLQQAFKAQGIGEGDRIAAMMPNMPETVAAMLAAASIGAIWSSCSPDFGEQGVL
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DRFGQIGPKLFIACDAYWYSGKLQDVGPKVAAVAKSLGVPTIVVHYAGDAEAVARATPGA
HHHHCCCCEEEEEEEEEEECCCCHHCCHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCH
STLETFVAPYQAREVEFTPLAFAHPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEQRLHC
HHHHHHHCCCCCCCEEECCCHHHCEEEEEEECCCCCCCHHEEECCCHHHHHHHHHHHHHC
GLKAGEKLFYFTTCGWMMWNWLVSGLASGATLCLFDGSPFAPDGNVLFDYAAAENFAIFG
CCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEE
TSAKYIDAVRKSGLTPRTSHDLSSLRLMTSTGSPLSPEGFTFVYEGIKADVQLASISGGT
CCHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCH
DIVSCFVLGNPLQPVWRGEIQGAGLGLAVDVWNDDGKPVRGEKGELVCTKAFPSMPVMFW
HHEEEEEECCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEE
NDPDGAKYRAAYFDRFDNVWCHGDFAEWTEHGGLVIHGRSDATLNPGGVRIGTAEIYNQV
ECCCCCCCHHHHHHHCCCEEECCCHHHHHHCCCEEEECCCCCEECCCCEEEEHHHHHHHH
EQMEEVAEALCIGQEWDDDVRVILFVRLAPGVTLTEDLIKAIKTRIRTGASPRHVPAKII
HHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
AVADIPRTKSGKIVELAVREVVHNRPVKNQEALANPEALRLFAGLEELKD
EEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDNRPLWVPSAEAVARSPIHAFMARCNADFGLSLSGFEDLHAWSVAERENFWSAVWDFC
CCCCCCEECCCHHHHHHCHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
GVKGERGAEVLINGDRMLEARFFPNATLNFAENLLAGRGDGDAIVFRGEDKAEDRWSWDR
CCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHH
LRALVSKLQQAFKAQGIGEGDRIAAMMPNMPETVAAMLAAASIGAIWSSCSPDFGEQGVL
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
DRFGQIGPKLFIACDAYWYSGKLQDVGPKVAAVAKSLGVPTIVVHYAGDAEAVARATPGA
HHHHCCCCEEEEEEEEEEECCCCHHCCHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCH
STLETFVAPYQAREVEFTPLAFAHPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEQRLHC
HHHHHHHCCCCCCCEEECCCHHHCEEEEEEECCCCCCCHHEEECCCHHHHHHHHHHHHHC
GLKAGEKLFYFTTCGWMMWNWLVSGLASGATLCLFDGSPFAPDGNVLFDYAAAENFAIFG
CCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEE
TSAKYIDAVRKSGLTPRTSHDLSSLRLMTSTGSPLSPEGFTFVYEGIKADVQLASISGGT
CCHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCH
DIVSCFVLGNPLQPVWRGEIQGAGLGLAVDVWNDDGKPVRGEKGELVCTKAFPSMPVMFW
HHEEEEEECCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEE
NDPDGAKYRAAYFDRFDNVWCHGDFAEWTEHGGLVIHGRSDATLNPGGVRIGTAEIYNQV
ECCCCCCCHHHHHHHCCCEEECCCHHHHHHCCCEEEECCCCCEECCCCEEEEHHHHHHHH
EQMEEVAEALCIGQEWDDDVRVILFVRLAPGVTLTEDLIKAIKTRIRTGASPRHVPAKII
HHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
AVADIPRTKSGKIVELAVREVVHNRPVKNQEALANPEALRLFAGLEELKD
EEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]