Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 209547973

GI number: 209547973

Start: 370842

End: 372278

Strand: Reverse

Name: puuA [H]

Synonym: Rleg2_0365

Alternate gene names: 209547973

Gene position: 372278-370842 (Counterclockwise)

Preceding gene: 209547978

Following gene: 209547972

Centisome position: 8.2

GC content: 61.73

Gene sequence:

>1437_bases
ATGTCCCCAAAAAAGACGACGCTGAAGCCTGCCAGAAACGTACCCGCCTCGAAGAAAACTCCCCCGGACCCCGGGTCTCT
GCGCGGCGTTGCGAACTGGAAGGAAGCAGCGCGGTGGCTGAGGGACAGGGGCATCGAAGACATCGAGTGCATCACGCCGG
ACCTTGCCGGCGTTCCGCGCGGCAAGATGATGCCGAGCTCGAAATTCACCTCGAACACCTCGCTGGCGCTGCCTTCGGCG
ATCTACCGGCATACGATTTCCGGCGAGTATCCGGAGGAGACAGAAAGCTTCCGCTACGAGCCGCGCGACAGCGACCTGAA
GCTGATGCCCGATCTTTCGACGCTTTCGGTGGTGCCCTGGGAGACCGACCCGACGGCACAGGTGATCTGCGACATCGTCG
ATTCCGACGGCGGCGAAGTGCCCTATACGCCGCGCAACGTGCTGAAGCGGATCATGAGCCTTTATCACGACCGTGGCTGG
AAGCCGATCGTGGCGCCGGAGATCGAATTCTACCTGGTCGCCAAGAATGACGACCCCGACTATCCGCTGCACCCGCCCAA
GGGCCGGTCCGGCCGCTCGATCCTCGGCGGCCAGGGCTATTCAATCGCCGGCATCAACGAATTCGACGAGTTGATCGACG
ACATTTACCATTTCTCGGAGAAGCAGGGTCTCGAGATCGATACGCTGATCCATGAAGAGGGACCGGCGCAGCTCGAGATC
AACCTGCGCCACGGCAATCCGATCGAACTTGCCGACCAGGTGTTCCTGTTCAAACGCACGATCCGCGAGGCGGCGTTGAA
GCACGACATTTATGCGACCTTCATGGCCAAGCCGATGCAGGCCCAGCCGGGTTCGGCGATGCATATCCATCAGTCGGTGG
TCAATATCGAAACGGGCAAGAACGTCTTCTCCAATCCGGACGGATCGGCCTCGAAGGAGTTCTTCCATTTCATCGGCGGC
ATGCAGAAATTCGTGCCGAGCGCGATGGCGATGCTGGCGCCCTATGTGAATTCCTACCGGCGCCTGCAGCCCGATATGTC
CTGCCCGGTCAACAATGCCTGGGGTTACGACAATCGGACGACCGCCTTCCGCGTGCCGGTATCCGATCCGCAGGCCAGGC
GCGTCGAAAACCGGCTGCCGAGCTCGGACGCCAATCCTTATCTGGCGCTTGCCGCATCGCTCGCCGCCGGCCTGCTCGGC
ATCATGAAGCAGATCGAGCCGACGGCGCCGACCGAGGATTCGGCCAATGAGGGCTCGATCGACCTGCCGCGCGGCCTGTT
GGAAGCCGTGGCGCTGCTCGAGGACGAGCCGGCTTTCGAGGAGATTTTCGGCAAGCAGTTCATCGGCCTCTACGCCGGCG
TCAAGCGCGGCGAATTCGAGACCTTCATGCAGGTGATCAGCCCCTGGGAGCGGGAATTCCTTCTGCTCAACGTGTGA

Upstream 100 bases:

>100_bases
CTTCTTTTTCCATCAAAGGGCGAAAGTCAACGTTCTCAACCTCTCGCCAATTGGCAAAGTCCGGTTATGATGGCGCCACG
ATCAACCAAACCGGTGTGTC

Downstream 100 bases:

>100_bases
GGCAGCGTCATGGCATCGCAGGAGACGTGGCAGAGCCCGATCTCGCCGGGCCTATCCTGGTATCAGGCAACCGCCGGGGA
GCGGCCGAGCTATGCCGCTC

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSPKKTTLKPARNVPASKKTPPDPGSLRGVANWKEAARWLRDRGIEDIECITPDLAGVPRGKMMPSSKFTSNTSLALPSA
IYRHTISGEYPEETESFRYEPRDSDLKLMPDLSTLSVVPWETDPTAQVICDIVDSDGGEVPYTPRNVLKRIMSLYHDRGW
KPIVAPEIEFYLVAKNDDPDYPLHPPKGRSGRSILGGQGYSIAGINEFDELIDDIYHFSEKQGLEIDTLIHEEGPAQLEI
NLRHGNPIELADQVFLFKRTIREAALKHDIYATFMAKPMQAQPGSAMHIHQSVVNIETGKNVFSNPDGSASKEFFHFIGG
MQKFVPSAMAMLAPYVNSYRRLQPDMSCPVNNAWGYDNRTTAFRVPVSDPQARRVENRLPSSDANPYLALAASLAAGLLG
IMKQIEPTAPTEDSANEGSIDLPRGLLEAVALLEDEPAFEEIFGKQFIGLYAGVKRGEFETFMQVISPWEREFLLLNV

Sequences:

>Translated_478_residues
MSPKKTTLKPARNVPASKKTPPDPGSLRGVANWKEAARWLRDRGIEDIECITPDLAGVPRGKMMPSSKFTSNTSLALPSA
IYRHTISGEYPEETESFRYEPRDSDLKLMPDLSTLSVVPWETDPTAQVICDIVDSDGGEVPYTPRNVLKRIMSLYHDRGW
KPIVAPEIEFYLVAKNDDPDYPLHPPKGRSGRSILGGQGYSIAGINEFDELIDDIYHFSEKQGLEIDTLIHEEGPAQLEI
NLRHGNPIELADQVFLFKRTIREAALKHDIYATFMAKPMQAQPGSAMHIHQSVVNIETGKNVFSNPDGSASKEFFHFIGG
MQKFVPSAMAMLAPYVNSYRRLQPDMSCPVNNAWGYDNRTTAFRVPVSDPQARRVENRLPSSDANPYLALAASLAAGLLG
IMKQIEPTAPTEDSANEGSIDLPRGLLEAVALLEDEPAFEEIFGKQFIGLYAGVKRGEFETFMQVISPWEREFLLLNV
>Mature_477_residues
SPKKTTLKPARNVPASKKTPPDPGSLRGVANWKEAARWLRDRGIEDIECITPDLAGVPRGKMMPSSKFTSNTSLALPSAI
YRHTISGEYPEETESFRYEPRDSDLKLMPDLSTLSVVPWETDPTAQVICDIVDSDGGEVPYTPRNVLKRIMSLYHDRGWK
PIVAPEIEFYLVAKNDDPDYPLHPPKGRSGRSILGGQGYSIAGINEFDELIDDIYHFSEKQGLEIDTLIHEEGPAQLEIN
LRHGNPIELADQVFLFKRTIREAALKHDIYATFMAKPMQAQPGSAMHIHQSVVNIETGKNVFSNPDGSASKEFFHFIGGM
QKFVPSAMAMLAPYVNSYRRLQPDMSCPVNNAWGYDNRTTAFRVPVSDPQARRVENRLPSSDANPYLALAASLAAGLLGI
MKQIEPTAPTEDSANEGSIDLPRGLLEAVALLEDEPAFEEIFGKQFIGLYAGVKRGEFETFMQVISPWEREFLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=425, Percent_Identity=27.2941176470588, Blast_Score=152, Evalue=6e-37,
Organism=Escherichia coli, GI87081870, Length=462, Percent_Identity=35.0649350649351, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1790301, Length=423, Percent_Identity=29.5508274231678, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 53220; Mature: 53089

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKKTTLKPARNVPASKKTPPDPGSLRGVANWKEAARWLRDRGIEDIECITPDLAGVPR
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCC
GKMMPSSKFTSNTSLALPSAIYRHTISGEYPEETESFRYEPRDSDLKLMPDLSTLSVVPW
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEECCCCCCEEEECC
ETDPTAQVICDIVDSDGGEVPYTPRNVLKRIMSLYHDRGWKPIVAPEIEFYLVAKNDDPD
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCC
YPLHPPKGRSGRSILGGQGYSIAGINEFDELIDDIYHFSEKQGLEIDTLIHEEGPAQLEI
CCCCCCCCCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEE
NLRHGNPIELADQVFLFKRTIREAALKHDIYATFMAKPMQAQPGSAMHIHQSVVNIETGK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHCCCC
NVFSNPDGSASKEFFHFIGGMQKFVPSAMAMLAPYVNSYRRLQPDMSCPVNNAWGYDNRT
CHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TAFRVPVSDPQARRVENRLPSSDANPYLALAASLAAGLLGIMKQIEPTAPTEDSANEGSI
EEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DLPRGLLEAVALLEDEPAFEEIFGKQFIGLYAGVKRGEFETFMQVISPWEREFLLLNV
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
SPKKTTLKPARNVPASKKTPPDPGSLRGVANWKEAARWLRDRGIEDIECITPDLAGVPR
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCC
GKMMPSSKFTSNTSLALPSAIYRHTISGEYPEETESFRYEPRDSDLKLMPDLSTLSVVPW
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEECCCCCCEEEECC
ETDPTAQVICDIVDSDGGEVPYTPRNVLKRIMSLYHDRGWKPIVAPEIEFYLVAKNDDPD
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCC
YPLHPPKGRSGRSILGGQGYSIAGINEFDELIDDIYHFSEKQGLEIDTLIHEEGPAQLEI
CCCCCCCCCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEE
NLRHGNPIELADQVFLFKRTIREAALKHDIYATFMAKPMQAQPGSAMHIHQSVVNIETGK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHCCCC
NVFSNPDGSASKEFFHFIGGMQKFVPSAMAMLAPYVNSYRRLQPDMSCPVNNAWGYDNRT
CHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TAFRVPVSDPQARRVENRLPSSDANPYLALAASLAAGLLGIMKQIEPTAPTEDSANEGSI
EEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DLPRGLLEAVALLEDEPAFEEIFGKQFIGLYAGVKRGEFETFMQVISPWEREFLLLNV
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]