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The map label for this gene is pcrA [H]

Identifier: 209547839

GI number: 209547839

Start: 237843

End: 239903

Strand: Reverse

Name: pcrA [H]

Synonym: Rleg2_0230

Alternate gene names: 209547839

Gene position: 239903-237843 (Counterclockwise)

Preceding gene: 209547840

Following gene: 209547833

Centisome position: 5.29

GC content: 65.07

Gene sequence:

>2061_bases
ATGTATCTCGAAAAGCTCAACCCCCAGCAGCGCATGGCCGTCGAGCATGGCACGCTCACCGACGGCAGCCATATTGCCGG
GCCGCTGCTGGTCATTGCCGGCGCCGGTTCGGGCAAGACCAATACGCTGGCGCATCGGGTCGCCCATCTGATCGTCAAGG
GCGCCGATCCGCGCCGCATCCTGCTGATGACCTTTTCGCGCCGGGCCGCCGCCGAGATGGCGCGGCGCGTCGAGCGCATC
TGCCGCGATGTGCTGGGTGCGAATGCCGGCGTCATGGCCGATGCGCTGAACTGGTCCGGCACCTTCCACGGCATCGGCGC
GCGGCTGTTGCGCGATTATGCGCAGCAGATCGGCCTCGACGCCGATTTCACCATCCATGACCGCGAAGACAGCGCCGACC
TGATCAACCTCATCCGGCACGATCTCGGCTTCTCCAAAACGGAAAGCCGGTTTCCGGCCAAAGGCACCTGCCTTGCGATC
TATTCCCGAGCGGTGAATTCCGAGACGGCGCTCGATCAGGTGCTGCGCGACGCCTTTCCCTGGTGCGCGGCCTGGGAGAA
ACAGCTGCGCGAGCTTTTCGCCTGTTATGTCGAGGCCAAGCAGAGCCAGAACGTGCTCGATTACGACGACCTCTTGCTCT
ACTGGGCGCAGATGGTCGGCGAAAGCGTGATTGCCGAGGATATCGGCAGCCGCTTCGACCATGTGCTGGTCGACGAATAT
CAGGACACCAACCGGCTGCAGGCCTCGATCCTGATGGCGCTGAAACCGCAAGGCCAGGGGCTGACCGTGGTCGGCGACGA
CGCGCAGTCGATCTATTCCTTCCGGGCAGCGACGGTGCGCAACATCCTCGATTTTCCCGCCGCCTTCAGCCCGGCCGCCA
ATATCGTCACGCTCGACCGCAATTACCGCTCGACGCAGCCGATCCTGGCGGCGGCCAATGCCGTCATCGATCTCGCCTCG
GAGCGCTTCACCAAGAACCTCTGGACCGAGCGGCAATCTGCCGAACGGCCGCGCCTGGTCACCGTGCGCGACGAGGCCGA
CCAGGCGCGTTATGTCGCCGACATGGTGCTCGACAATCGCGAGGAAGGCCTGACGCTCAAGCAGCAGGCGGTGCTCTTTC
GCGCCTCGCATCACAGCGGACCGCTGGAGGTCGAGCTGACCCGGCGCAACATTCCCTTCGTCAAGTTCGGCGGGCTGAAA
TTTCTCGACAGCGCCCATGTCAAGGACATGCTGGCGGCGCTGCGCTTCGCACAGAACCCGCGCGACCGGGTGGCAGGCTT
CCGGCTGATGCAGATCCTGCCGGGCGTCGGGCCATCGACGGCGCAGAAGGCGCTCGACCTGATGGCCGAGGATGCGAGCC
CGATCACGGCGCTCGCCGCCATGCCGGCGCCACCGCGCTCCGGTGAGGACTGGACTGATTTCGTTTCCATGCTGCAGGAG
ATGAAATCCGGCAAGGCCGGCTGGCCGGCGGAAATCGAGCTGGCGCGGCAATGGTATGCGCCGCATCTGGAGCGGCTGCA
TGAAGATGCGGCGACGCGGCAGGCCGATCTGCTGCAGCTCGAGCAGATTGCCGGCGGTTATGCCTCGCGCGAGCGTTTCC
TCACCGAACTCACCCTCGATCCGCCGGATGCGACTAGCGACCAGGCCGGCGTGCCGCTGCTTGACGAGGATTACCTCATT
CTTTCCACCATCCATTCCGCCAAGGGCCAGGAATGGACAAGGGTCTTCATGCTGAACGTCGTCGACGGCTGCATTCCGAG
CGATCTTGCCGTCGGCGCCAGCGCCGAAATCGAGGAGGAACGCCGGCTGCTCTATGTGGCGATGACCCGCGCCCGCGACG
GTCTCGACCTTGTGGTGCCGCAGCGCTTCTTCACCTATGGGCAGAATGCGCAGGGCGACCGGCACGTCTATGCCTCGCGC
AGCCGCTTCATTCCGGCAACGCTGCTGCAGTTCTTCGAAGCCTGTAGCTGGCCGCAGGTGAAATCCGAGGCGGCCGCGCA
AGCGCAGGCGCGGCAGGTGCGCATCGATGTCGGCGCCCGCATGCGCGGCATGTGGCGATAA

Upstream 100 bases:

>100_bases
CCCGGACACCTCAATGCTTGACAGCGGCTGCAATCGCGAACATTCAAGGAACATCTAATCTTTCCGCAGTGATTCTGCGG
GAGCCCGACCGGACGGCTGG

Downstream 100 bases:

>100_bases
GCAAACGAAGTCAGAGTGATAGCGGCGGCTCGGCCGGTTTCAGGAAGCGCTCGACCATTGCCGGTTCCACAGCCTCGACA
CTTGCCGGCGACCATTTCGG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MYLEKLNPQQRMAVEHGTLTDGSHIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRILLMTFSRRAAAEMARRVERI
CRDVLGANAGVMADALNWSGTFHGIGARLLRDYAQQIGLDADFTIHDREDSADLINLIRHDLGFSKTESRFPAKGTCLAI
YSRAVNSETALDQVLRDAFPWCAAWEKQLRELFACYVEAKQSQNVLDYDDLLLYWAQMVGESVIAEDIGSRFDHVLVDEY
QDTNRLQASILMALKPQGQGLTVVGDDAQSIYSFRAATVRNILDFPAAFSPAANIVTLDRNYRSTQPILAAANAVIDLAS
ERFTKNLWTERQSAERPRLVTVRDEADQARYVADMVLDNREEGLTLKQQAVLFRASHHSGPLEVELTRRNIPFVKFGGLK
FLDSAHVKDMLAALRFAQNPRDRVAGFRLMQILPGVGPSTAQKALDLMAEDASPITALAAMPAPPRSGEDWTDFVSMLQE
MKSGKAGWPAEIELARQWYAPHLERLHEDAATRQADLLQLEQIAGGYASRERFLTELTLDPPDATSDQAGVPLLDEDYLI
LSTIHSAKGQEWTRVFMLNVVDGCIPSDLAVGASAEIEEERRLLYVAMTRARDGLDLVVPQRFFTYGQNAQGDRHVYASR
SRFIPATLLQFFEACSWPQVKSEAAAQAQARQVRIDVGARMRGMWR

Sequences:

>Translated_686_residues
MYLEKLNPQQRMAVEHGTLTDGSHIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRILLMTFSRRAAAEMARRVERI
CRDVLGANAGVMADALNWSGTFHGIGARLLRDYAQQIGLDADFTIHDREDSADLINLIRHDLGFSKTESRFPAKGTCLAI
YSRAVNSETALDQVLRDAFPWCAAWEKQLRELFACYVEAKQSQNVLDYDDLLLYWAQMVGESVIAEDIGSRFDHVLVDEY
QDTNRLQASILMALKPQGQGLTVVGDDAQSIYSFRAATVRNILDFPAAFSPAANIVTLDRNYRSTQPILAAANAVIDLAS
ERFTKNLWTERQSAERPRLVTVRDEADQARYVADMVLDNREEGLTLKQQAVLFRASHHSGPLEVELTRRNIPFVKFGGLK
FLDSAHVKDMLAALRFAQNPRDRVAGFRLMQILPGVGPSTAQKALDLMAEDASPITALAAMPAPPRSGEDWTDFVSMLQE
MKSGKAGWPAEIELARQWYAPHLERLHEDAATRQADLLQLEQIAGGYASRERFLTELTLDPPDATSDQAGVPLLDEDYLI
LSTIHSAKGQEWTRVFMLNVVDGCIPSDLAVGASAEIEEERRLLYVAMTRARDGLDLVVPQRFFTYGQNAQGDRHVYASR
SRFIPATLLQFFEACSWPQVKSEAAAQAQARQVRIDVGARMRGMWR
>Mature_686_residues
MYLEKLNPQQRMAVEHGTLTDGSHIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRILLMTFSRRAAAEMARRVERI
CRDVLGANAGVMADALNWSGTFHGIGARLLRDYAQQIGLDADFTIHDREDSADLINLIRHDLGFSKTESRFPAKGTCLAI
YSRAVNSETALDQVLRDAFPWCAAWEKQLRELFACYVEAKQSQNVLDYDDLLLYWAQMVGESVIAEDIGSRFDHVLVDEY
QDTNRLQASILMALKPQGQGLTVVGDDAQSIYSFRAATVRNILDFPAAFSPAANIVTLDRNYRSTQPILAAANAVIDLAS
ERFTKNLWTERQSAERPRLVTVRDEADQARYVADMVLDNREEGLTLKQQAVLFRASHHSGPLEVELTRRNIPFVKFGGLK
FLDSAHVKDMLAALRFAQNPRDRVAGFRLMQILPGVGPSTAQKALDLMAEDASPITALAAMPAPPRSGEDWTDFVSMLQE
MKSGKAGWPAEIELARQWYAPHLERLHEDAATRQADLLQLEQIAGGYASRERFLTELTLDPPDATSDQAGVPLLDEDYLI
LSTIHSAKGQEWTRVFMLNVVDGCIPSDLAVGASAEIEEERRLLYVAMTRARDGLDLVVPQRFFTYGQNAQGDRHVYASR
SRFIPATLLQFFEACSWPQVKSEAAAQAQARQVRIDVGARMRGMWR

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=683, Percent_Identity=30.7467057101025, Blast_Score=259, Evalue=5e-70,
Organism=Escherichia coli, GI48994965, Length=664, Percent_Identity=28.4638554216867, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322369, Length=529, Percent_Identity=28.1663516068053, Blast_Score=146, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6324477, Length=416, Percent_Identity=23.5576923076923, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76322; Mature: 76322

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLEKLNPQQRMAVEHGTLTDGSHIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRI
CCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
LLMTFSRRAAAEMARRVERICRDVLGANAGVMADALNWSGTFHGIGARLLRDYAQQIGLD
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
ADFTIHDREDSADLINLIRHDLGFSKTESRFPAKGTCLAIYSRAVNSETALDQVLRDAFP
CCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
WCAAWEKQLRELFACYVEAKQSQNVLDYDDLLLYWAQMVGESVIAEDIGSRFDHVLVDEY
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
QDTNRLQASILMALKPQGQGLTVVGDDAQSIYSFRAATVRNILDFPAAFSPAANIVTLDR
CCHHHHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
NYRSTQPILAAANAVIDLASERFTKNLWTERQSAERPRLVTVRDEADQARYVADMVLDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCC
EEGLTLKQQAVLFRASHHSGPLEVELTRRNIPFVKFGGLKFLDSAHVKDMLAALRFAQNP
CCCCCHHHHHHHHEECCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCC
RDRVAGFRLMQILPGVGPSTAQKALDLMAEDASPITALAAMPAPPRSGEDWTDFVSMLQE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
MKSGKAGWPAEIELARQWYAPHLERLHEDAATRQADLLQLEQIAGGYASRERFLTELTLD
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECC
PPDATSDQAGVPLLDEDYLILSTIHSAKGQEWTRVFMLNVVDGCIPSDLAVGASAEIEEE
CCCCCCCCCCCCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RRLLYVAMTRARDGLDLVVPQRFFTYGQNAQGDRHVYASRSRFIPATLLQFFEACSWPQV
HHEEEEEEHHCCCCCEEEECHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCHH
KSEAAAQAQARQVRIDVGARMRGMWR
HHHHHHHHHHHEEEEECCCCCCCCCC
>Mature Secondary Structure
MYLEKLNPQQRMAVEHGTLTDGSHIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRI
CCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
LLMTFSRRAAAEMARRVERICRDVLGANAGVMADALNWSGTFHGIGARLLRDYAQQIGLD
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
ADFTIHDREDSADLINLIRHDLGFSKTESRFPAKGTCLAIYSRAVNSETALDQVLRDAFP
CCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
WCAAWEKQLRELFACYVEAKQSQNVLDYDDLLLYWAQMVGESVIAEDIGSRFDHVLVDEY
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
QDTNRLQASILMALKPQGQGLTVVGDDAQSIYSFRAATVRNILDFPAAFSPAANIVTLDR
CCHHHHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
NYRSTQPILAAANAVIDLASERFTKNLWTERQSAERPRLVTVRDEADQARYVADMVLDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCC
EEGLTLKQQAVLFRASHHSGPLEVELTRRNIPFVKFGGLKFLDSAHVKDMLAALRFAQNP
CCCCCHHHHHHHHEECCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCC
RDRVAGFRLMQILPGVGPSTAQKALDLMAEDASPITALAAMPAPPRSGEDWTDFVSMLQE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
MKSGKAGWPAEIELARQWYAPHLERLHEDAATRQADLLQLEQIAGGYASRERFLTELTLD
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECC
PPDATSDQAGVPLLDEDYLILSTIHSAKGQEWTRVFMLNVVDGCIPSDLAVGASAEIEEE
CCCCCCCCCCCCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RRLLYVAMTRARDGLDLVVPQRFFTYGQNAQGDRHVYASRSRFIPATLLQFFEACSWPQV
HHEEEEEEHHCCCCCEEEECHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCHH
KSEAAAQAQARQVRIDVGARMRGMWR
HHHHHHHHHHHEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]