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The map label for this gene is fadA [H]

Identifier: 209547823

GI number: 209547823

Start: 216691

End: 217899

Strand: Direct

Name: fadA [H]

Synonym: Rleg2_0214

Alternate gene names: 209547823

Gene position: 216691-217899 (Clockwise)

Preceding gene: 209547822

Following gene: 209547824

Centisome position: 4.78

GC content: 64.76

Gene sequence:

>1209_bases
ATGACCGAGGTTTTCATTTACGACCACGTCCGCACGCCGCGCGGCCGCGGCAAGAAGGATGGCGCCCTGCATGAGGTGCC
GTCCGTCCGCCTGGCGGCCAAGACGCTGGAAGCAATCCGCGACCGCAACGGGCTGGACACTGCAACCGTCGACGACATCA
TCATGGGCTGCGTCGATCCGGTCATGGATGCCGGCGCCGTCATCCCCAAGGCCGCTGCCTTCGAGGCCGGTTATTCCACC
CGGGCGCCCGGAATGCAGATCTCCCGCTTTTGCGCCTCCGGCCTCGATGCCGTCAATTTCGCCGCCGGCAAGATTGCCCA
AGGGGCCGACGATATCGTCATCGCGGGCGGCGTCGAAAGCATGTCCCGCGTCGGCCTCGGCATGTCCGGCGGCGCCTGGT
TCATGGACCCCTCGGTGAATTTCCCGGCCTATTTCATGCCGCAGGGCGTCTCGGCCGATCTCATCGCCACCAAATACGGT
TTCTCCAGAACCGATGTCGACGCCTACGCCGTCGAGAGCCAGAAGCGCGCCGGACATGCCTGGGCCCAGGGCTGGTTCGA
CAGATCGGTCGTTCCGGTCAAGGACCAGAACGGCCTGACGATCCTGGATAAGGATGAGCATATGCGCCCCGGCACCGACA
TGCAGGCGCTCGCCTCCCTCAATCCCTCCTTCCAGATGCCCGGCGAGATGGGCGGCTTCGAAGCCGTCGGCATCCAGGCC
CATCCGGAAATCGAGCGTATCAACTATGTCCACCATGCCGGCAATTCCTCCGGCATCGTCGATGGTGCCGGCGTCGTTCT
GCTGGGCTCCAAGGCCGGCGGCGAAAGCATGGGGCTGAAGCCGCGCGCCCGCATCAAGGCCTTCGCCAATATCGGCTCCG
ATCCGGCCCTGATGCTGACCGGACCCGTCGATGTCACCGAGAAGCTGTTGAAGCGGACCGGCATGCGCCTTGCCGATATC
GACCTCTTCGAACTGAACGAGGCCTTCGCCGCAGTCGTGCTGCGCTACATGCAGGCCTTCGACATCGATCACGACACGAT
CAACGTCAATGGCGGCGCCATCGCCATGGGCCATCCGCTCGGCGCCACCGGCGCGATGATCCTCGGCACGGTGCTCGACG
AGCTCGAACGCCGCGATTTGAACACCGCGCTGGTGACGCTCTGCATCGGCGCCGGCATGGGCACGGCAACGGTTATCGAA
AGGGTCTGA

Upstream 100 bases:

>100_bases
CCGCGAGCGCGCCCTCATCCGACCTTCGGCCACCTTCTCCCGAGGGGAGAAGGTGAGTTGAGATTTACAGGCGCCGCCGC
GCCGCCAGGGAGACGAGAAT

Downstream 100 bases:

>100_bases
CGATGAGCTTCAACACCGGCACGATCGAAACCGAATATGCCGAGGTCACCGAGCACTGGTCGCCTCGCGTAATCGCTGAT
CTCAACGGCCAGAGCGTCAA

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTEVFIYDHVRTPRGRGKKDGALHEVPSVRLAAKTLEAIRDRNGLDTATVDDIIMGCVDPVMDAGAVIPKAAAFEAGYST
RAPGMQISRFCASGLDAVNFAAGKIAQGADDIVIAGGVESMSRVGLGMSGGAWFMDPSVNFPAYFMPQGVSADLIATKYG
FSRTDVDAYAVESQKRAGHAWAQGWFDRSVVPVKDQNGLTILDKDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIQA
HPEIERINYVHHAGNSSGIVDGAGVVLLGSKAGGESMGLKPRARIKAFANIGSDPALMLTGPVDVTEKLLKRTGMRLADI
DLFELNEAFAAVVLRYMQAFDIDHDTINVNGGAIAMGHPLGATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIE
RV

Sequences:

>Translated_402_residues
MTEVFIYDHVRTPRGRGKKDGALHEVPSVRLAAKTLEAIRDRNGLDTATVDDIIMGCVDPVMDAGAVIPKAAAFEAGYST
RAPGMQISRFCASGLDAVNFAAGKIAQGADDIVIAGGVESMSRVGLGMSGGAWFMDPSVNFPAYFMPQGVSADLIATKYG
FSRTDVDAYAVESQKRAGHAWAQGWFDRSVVPVKDQNGLTILDKDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIQA
HPEIERINYVHHAGNSSGIVDGAGVVLLGSKAGGESMGLKPRARIKAFANIGSDPALMLTGPVDVTEKLLKRTGMRLADI
DLFELNEAFAAVVLRYMQAFDIDHDTINVNGGAIAMGHPLGATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIE
RV
>Mature_401_residues
TEVFIYDHVRTPRGRGKKDGALHEVPSVRLAAKTLEAIRDRNGLDTATVDDIIMGCVDPVMDAGAVIPKAAAFEAGYSTR
APGMQISRFCASGLDAVNFAAGKIAQGADDIVIAGGVESMSRVGLGMSGGAWFMDPSVNFPAYFMPQGVSADLIATKYGF
SRTDVDAYAVESQKRAGHAWAQGWFDRSVVPVKDQNGLTILDKDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIQAH
PEIERINYVHHAGNSSGIVDGAGVVLLGSKAGGESMGLKPRARIKAFANIGSDPALMLTGPVDVTEKLLKRTGMRLADID
LFELNEAFAAVVLRYMQAFDIDHDTINVNGGAIAMGHPLGATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIER
V

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=411, Percent_Identity=31.8734793187348, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI148539872, Length=407, Percent_Identity=33.1695331695332, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4501853, Length=410, Percent_Identity=32.9268292682927, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI4557237, Length=418, Percent_Identity=29.4258373205742, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4504327, Length=446, Percent_Identity=29.1479820627803, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI194353979, Length=124, Percent_Identity=36.2903225806452, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=38.4976525821596, Blast_Score=247, Evalue=9e-67,
Organism=Escherichia coli, GI48994986, Length=413, Percent_Identity=37.772397094431, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI87082165, Length=417, Percent_Identity=38.1294964028777, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788554, Length=419, Percent_Identity=34.6062052505967, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788683, Length=444, Percent_Identity=26.5765765765766, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI133906874, Length=413, Percent_Identity=31.7191283292978, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17535921, Length=419, Percent_Identity=30.3102625298329, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147385, Length=410, Percent_Identity=29.2682926829268, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17551802, Length=450, Percent_Identity=28, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=25.3731343283582, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322031, Length=358, Percent_Identity=33.5195530726257, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6325229, Length=414, Percent_Identity=29.7101449275362, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24655093, Length=415, Percent_Identity=35.1807228915663, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17648125, Length=397, Percent_Identity=33.2493702770781, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24640423, Length=418, Percent_Identity=31.1004784688995, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI17137578, Length=448, Percent_Identity=28.7946428571429, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42524; Mature: 42393

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVFIYDHVRTPRGRGKKDGALHEVPSVRLAAKTLEAIRDRNGLDTATVDDIIMGCVDP
CCEEEEEECCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VMDAGAVIPKAAAFEAGYSTRAPGMQISRFCASGLDAVNFAAGKIAQGADDIVIAGGVES
HHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCHHH
MSRVGLGMSGGAWFMDPSVNFPAYFMPQGVSADLIATKYGFSRTDVDAYAVESQKRAGHA
HHHHCCCCCCCEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHH
WAQGWFDRSVVPVKDQNGLTILDKDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIQA
HHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECC
HPEIERINYVHHAGNSSGIVDGAGVVLLGSKAGGESMGLKPRARIKAFANIGSDPALMLT
CCCHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GPVDVTEKLLKRTGMRLADIDLFELNEAFAAVVLRYMQAFDIDHDTINVNGGAIAMGHPL
CCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCC
GATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIERV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TEVFIYDHVRTPRGRGKKDGALHEVPSVRLAAKTLEAIRDRNGLDTATVDDIIMGCVDP
CEEEEEECCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VMDAGAVIPKAAAFEAGYSTRAPGMQISRFCASGLDAVNFAAGKIAQGADDIVIAGGVES
HHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCHHH
MSRVGLGMSGGAWFMDPSVNFPAYFMPQGVSADLIATKYGFSRTDVDAYAVESQKRAGHA
HHHHCCCCCCCEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHH
WAQGWFDRSVVPVKDQNGLTILDKDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIQA
HHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECC
HPEIERINYVHHAGNSSGIVDGAGVVLLGSKAGGESMGLKPRARIKAFANIGSDPALMLT
CCCHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GPVDVTEKLLKRTGMRLADIDLFELNEAFAAVVLRYMQAFDIDHDTINVNGGAIAMGHPL
CCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCC
GATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIERV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]