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The map label for this gene is dinF [H]

Identifier: 209547803

GI number: 209547803

Start: 193230

End: 194570

Strand: Reverse

Name: dinF [H]

Synonym: Rleg2_0194

Alternate gene names: 209547803

Gene position: 194570-193230 (Counterclockwise)

Preceding gene: 209547804

Following gene: 209547800

Centisome position: 4.29

GC content: 65.25

Gene sequence:

>1341_bases
ATGGATACGCACAATAACAGGTTCGCCTTCGAGGTGACGCACCGGCTGGTGCTGTCGATCGCCATTCCGATGACGCTCGG
CTTCATGACGACGCCGATGCTCGGGCTGACCAACACCGCCGTCGTCGGCCGTATAGGCGACCCGGAAGCGCTGGCGGGGC
TGGCGATCGGTGCGATGCTGTTCGATCTGATCATGGGCAGCTTCAATTTCCTGCGCGCCTCGACGACCGGACTGACGGCG
CAGGCCTATGGCCGGCGCGATCAGCACGAGCAGCAGGCGGTCTTCTCCCGGGCGCTGATTTCGGCGCTCGGCTGCGGGCT
GGCGCTGCTCTGTCTTTCGCCGCTGCTGAATGCGGCGGGCTTGCGGCTGATGGGGGCGGAAGGCGCAATCGCTGAGGCGA
CCAGCACCTATTTCTCGATCCGGATTCTCGCTGCACCGGCTGCGCTCGCCAATTACGCCATCCTCGGCTTCGTGCTCGGG
CGCGGACAGGGCAAGATCGGGCTGCTGCTGCAGGCGATCATCAACGGCATCAATATCCTGCTGTCGATCTATCTCGGTCT
GACCCTCGGCTGGGGCGTGGCGGGGGTCGCCTGGGGAACGATGGCCGGCGAGACGGCCGGCGCCCTTGCCGGGCTTTTCA
TCGTGCTTAGCGGCTTTGCCAAGGCCGAGCGGCCGGCATGGGCGGAGATCTCTTCGCGGCATCGGCTGGCCGAGCTTTTC
GCCCTCAACCGCGACATCCTGATCCGCACCTTCGTGCTGATCGGCGCCTTCACCATCATGACGCGGATCGGCACCAGTTT
CGGCGCCGTCACCCTTGCCGCCAATGCCGTGCTGATGAATTTCGTCCTGCTCTCCAGCTATTATCTCGACGGGCTTGCCA
ATGCCGCCGAGCAGATCACCGGCCGGGCGATCGGCGCACGTTACCGGCCGGCCTTCGACCGCGGGCTGAAGCTGACGATC
CTGTGGTCCTTCGGCCTGGCAGCGATCGTTTCCGCCTTTTTCTTCCTCGCCGGTCCCTGGCTGATCTCGGTGCTGACGAC
CTCACCCGAGGTGCGGCAGGCGGCAGCGGCCTATCTGCCCTGGGCGGCGGTGACCGGGTTGACCGGCGCGCTCGCCTTCC
TGATGGACGGGGTCTTCATCGGCGCCACATGGTCGGCCGAGATGCGCAACCGGATGCTGATGTCCTTTGCCGGCTATCTG
CTGATGCTCGCCGTCTTCGTGCCGCTCTTCGGCAATCACGGCCTGTGGCTTGCTATGAACGCCTTTCTGCTCTTCCGCGG
CTTTTTCCTGGCGCTGCTGGTCAAGCCGCGGGCCGATCAGACTTTTCGCGCCGCCCAGTAA

Upstream 100 bases:

>100_bases
TCGGCGTCGCCGTCGCCCACAATTCATCTTCCCGGAACCTGCCCTATTGGGCCATGGTGCTGTCTAGCTGAGGCGATTCG
GCCTCGATATCAGGCCATGG

Downstream 100 bases:

>100_bases
TCCAGCCTGCTGTCGCGCAGCTCGCCGATCCCGGCCGCCTTTTCGCGGTCCATGAGTTTGGAAAGGCCGCGGACGATATC
GCCGGGCAGACCGGGGCCTT

Product: MATE efflux family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MDTHNNRFAFEVTHRLVLSIAIPMTLGFMTTPMLGLTNTAVVGRIGDPEALAGLAIGAMLFDLIMGSFNFLRASTTGLTA
QAYGRRDQHEQQAVFSRALISALGCGLALLCLSPLLNAAGLRLMGAEGAIAEATSTYFSIRILAAPAALANYAILGFVLG
RGQGKIGLLLQAIINGINILLSIYLGLTLGWGVAGVAWGTMAGETAGALAGLFIVLSGFAKAERPAWAEISSRHRLAELF
ALNRDILIRTFVLIGAFTIMTRIGTSFGAVTLAANAVLMNFVLLSSYYLDGLANAAEQITGRAIGARYRPAFDRGLKLTI
LWSFGLAAIVSAFFFLAGPWLISVLTTSPEVRQAAAAYLPWAAVTGLTGALAFLMDGVFIGATWSAEMRNRMLMSFAGYL
LMLAVFVPLFGNHGLWLAMNAFLLFRGFFLALLVKPRADQTFRAAQ

Sequences:

>Translated_446_residues
MDTHNNRFAFEVTHRLVLSIAIPMTLGFMTTPMLGLTNTAVVGRIGDPEALAGLAIGAMLFDLIMGSFNFLRASTTGLTA
QAYGRRDQHEQQAVFSRALISALGCGLALLCLSPLLNAAGLRLMGAEGAIAEATSTYFSIRILAAPAALANYAILGFVLG
RGQGKIGLLLQAIINGINILLSIYLGLTLGWGVAGVAWGTMAGETAGALAGLFIVLSGFAKAERPAWAEISSRHRLAELF
ALNRDILIRTFVLIGAFTIMTRIGTSFGAVTLAANAVLMNFVLLSSYYLDGLANAAEQITGRAIGARYRPAFDRGLKLTI
LWSFGLAAIVSAFFFLAGPWLISVLTTSPEVRQAAAAYLPWAAVTGLTGALAFLMDGVFIGATWSAEMRNRMLMSFAGYL
LMLAVFVPLFGNHGLWLAMNAFLLFRGFFLALLVKPRADQTFRAAQ
>Mature_446_residues
MDTHNNRFAFEVTHRLVLSIAIPMTLGFMTTPMLGLTNTAVVGRIGDPEALAGLAIGAMLFDLIMGSFNFLRASTTGLTA
QAYGRRDQHEQQAVFSRALISALGCGLALLCLSPLLNAAGLRLMGAEGAIAEATSTYFSIRILAAPAALANYAILGFVLG
RGQGKIGLLLQAIINGINILLSIYLGLTLGWGVAGVAWGTMAGETAGALAGLFIVLSGFAKAERPAWAEISSRHRLAELF
ALNRDILIRTFVLIGAFTIMTRIGTSFGAVTLAANAVLMNFVLLSSYYLDGLANAAEQITGRAIGARYRPAFDRGLKLTI
LWSFGLAAIVSAFFFLAGPWLISVLTTSPEVRQAAAAYLPWAAVTGLTGALAFLMDGVFIGATWSAEMRNRMLMSFAGYL
LMLAVFVPLFGNHGLWLAMNAFLLFRGFFLALLVKPRADQTFRAAQ

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=425, Percent_Identity=36.9411764705882, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 47534; Mature: 47534

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHNNRFAFEVTHRLVLSIAIPMTLGFMTTPMLGLTNTAVVGRIGDPEALAGLAIGAML
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
FDLIMGSFNFLRASTTGLTAQAYGRRDQHEQQAVFSRALISALGCGLALLCLSPLLNAAG
HHHHHCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LRLMGAEGAIAEATSTYFSIRILAAPAALANYAILGFVLGRGQGKIGLLLQAIINGINIL
CEEECCCCCHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LSIYLGLTLGWGVAGVAWGTMAGETAGALAGLFIVLSGFAKAERPAWAEISSRHRLAELF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALNRDILIRTFVLIGAFTIMTRIGTSFGAVTLAANAVLMNFVLLSSYYLDGLANAAEQIT
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAIGARYRPAFDRGLKLTILWSFGLAAIVSAFFFLAGPWLISVLTTSPEVRQAAAAYLP
HHHHCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
WAAVTGLTGALAFLMDGVFIGATWSAEMRNRMLMSFAGYLLMLAVFVPLFGNHGLWLAMN
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AFLLFRGFFLALLVKPRADQTFRAAQ
HHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDTHNNRFAFEVTHRLVLSIAIPMTLGFMTTPMLGLTNTAVVGRIGDPEALAGLAIGAML
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
FDLIMGSFNFLRASTTGLTAQAYGRRDQHEQQAVFSRALISALGCGLALLCLSPLLNAAG
HHHHHCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LRLMGAEGAIAEATSTYFSIRILAAPAALANYAILGFVLGRGQGKIGLLLQAIINGINIL
CEEECCCCCHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LSIYLGLTLGWGVAGVAWGTMAGETAGALAGLFIVLSGFAKAERPAWAEISSRHRLAELF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALNRDILIRTFVLIGAFTIMTRIGTSFGAVTLAANAVLMNFVLLSSYYLDGLANAAEQIT
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAIGARYRPAFDRGLKLTILWSFGLAAIVSAFFFLAGPWLISVLTTSPEVRQAAAAYLP
HHHHCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
WAAVTGLTGALAFLMDGVFIGATWSAEMRNRMLMSFAGYLLMLAVFVPLFGNHGLWLAMN
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AFLLFRGFFLALLVKPRADQTFRAAQ
HHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]