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The map label for this gene is phoB [H]

Identifier: 209547782

GI number: 209547782

Start: 172420

End: 173103

Strand: Direct

Name: phoB [H]

Synonym: Rleg2_0173

Alternate gene names: 209547782

Gene position: 172420-173103 (Clockwise)

Preceding gene: 209547781

Following gene: 209547784

Centisome position: 3.8

GC content: 64.77

Gene sequence:

>684_bases
ATGATCCCGAGAGTTGCAGTTGTTGAAGACGAAGAGGCCCTGAGCGTGCTTCTTCGCTACAATCTCGAAGCTGAGGGCTT
CGAGGTCGATACCATCCTTCGTGGCGACGAGGCCGAAATCAGGCTTCAGGAGCGCACGCCCGACCTTCTCATCCTCGACT
GGATGCTGCCCGGCGTCTCCGGCATCGAGCTCTGCCGGCGCCTGCGCATGCGGCCGGAAACTGAGCGCCTGCCGATCATC
ATGCTGACGGCGCGCGGCGAAGAGAGCGAGCGCGTCCGCGGGCTTTCGACCGGGGCCGACGATTACGTCGTCAAGCCTTT
CTCGACCCCGGAACTCGTCGCCCGCGTCAAGGCGATGCTGCGCCGCGCCCGCCCCGAAGTGCTGTCGACCGTGCTGAAAT
GCGGTGATATCGAACTCGACCGCGAAACCCATCGCGTCCACCGCAAGAGCCGCGAAGTCCGCCTCGGCCCGACCGAATTC
CGCCTGCTGGAATTCCTGATGTCGTCGCCCGGCCGGGTCTTCTCCCGCTCGCAGCTGCTCGACGGCGTCTGGGGCCACGA
CATCTATGTCGACGAACGCACCGTCGACGTCCATGTCGGCCGCCTGCGCAAGGCGCTGAACTTCTCCAACATGCAGGACG
TCATCCGCACCGTCCGCGGCGCCGGTTATTCGATGGAAGCCTGA

Upstream 100 bases:

>100_bases
GGGCGCGCAAAGAGGTCGCAGCCATTTTTCTGATTTTGCGCAGGATGCATTGCTTAAATCACCCCCGGTTTAAGGATCAT
GCGCGAGGAGACCAACACGC

Downstream 100 bases:

>100_bases
GGGGATTCTGACAAAGTCGAGCAAGCTCCGCCCCTCACCCTAACCGACCGGGGTCGAGCCACACGTCTCGACCCGTCCTT
CGGACCCCCGTAAACGGGGC

Product: PhoB family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MIPRVAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERTPDLLILDWMLPGVSGIELCRRLRMRPETERLPII
MLTARGEESERVRGLSTGADDYVVKPFSTPELVARVKAMLRRARPEVLSTVLKCGDIELDRETHRVHRKSREVRLGPTEF
RLLEFLMSSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSNMQDVIRTVRGAGYSMEA

Sequences:

>Translated_227_residues
MIPRVAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERTPDLLILDWMLPGVSGIELCRRLRMRPETERLPII
MLTARGEESERVRGLSTGADDYVVKPFSTPELVARVKAMLRRARPEVLSTVLKCGDIELDRETHRVHRKSREVRLGPTEF
RLLEFLMSSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSNMQDVIRTVRGAGYSMEA
>Mature_227_residues
MIPRVAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERTPDLLILDWMLPGVSGIELCRRLRMRPETERLPII
MLTARGEESERVRGLSTGADDYVVKPFSTPELVARVKAMLRRARPEVLSTVLKCGDIELDRETHRVHRKSREVRLGPTEF
RLLEFLMSSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSNMQDVIRTVRGAGYSMEA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=49.3273542600897, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=43.3628318584071, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788394, Length=230, Percent_Identity=40.8695652173913, Blast_Score=156, Evalue=9e-40,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=37.219730941704, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=39.0350877192982, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=36.1233480176211, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=35.2678571428571, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=36.4864864864865, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=35.8744394618834, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=33.4821428571429, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=33.1896551724138, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=36.9565217391304, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787229, Length=234, Percent_Identity=32.4786324786325, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=31.1965811965812, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1788550, Length=144, Percent_Identity=31.25, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=33.6283185840708, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25998; Mature: 25998

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRVAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERTPDLLILDWMLPGVS
CCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCC
GIELCRRLRMRPETERLPIIMLTARGEESERVRGLSTGADDYVVKPFSTPELVARVKAML
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHH
RRARPEVLSTVLKCGDIELDRETHRVHRKSREVRLGPTEFRLLEFLMSSPGRVFSRSQLL
HHCCHHHHHHHHHHCCCEECHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
DGVWGHDIYVDERTVDVHVGRLRKALNFSNMQDVIRTVRGAGYSMEA
CCCCCCEEEEECCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIPRVAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQERTPDLLILDWMLPGVS
CCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCC
GIELCRRLRMRPETERLPIIMLTARGEESERVRGLSTGADDYVVKPFSTPELVARVKAML
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHH
RRARPEVLSTVLKCGDIELDRETHRVHRKSREVRLGPTEFRLLEFLMSSPGRVFSRSQLL
HHCCHHHHHHHHHHCCCEECHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
DGVWGHDIYVDERTVDVHVGRLRKALNFSNMQDVIRTVRGAGYSMEA
CCCCCCEEEEECCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]