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The map label for this gene is gloA [H]

Identifier: 209547752

GI number: 209547752

Start: 136828

End: 137223

Strand: Reverse

Name: gloA [H]

Synonym: Rleg2_0141

Alternate gene names: 209547752

Gene position: 137223-136828 (Counterclockwise)

Preceding gene: 209547753

Following gene: 209547751

Centisome position: 3.02

GC content: 59.85

Gene sequence:

>396_bases
TTGGCAAAGATGATCCACTCGATGATCCGCGTTCTCGACGAGGCGCGCTCGGTCGAATTCTATCGCAAGGCCTTCGGTCT
TTCGGTCGCCGACCGCGTCGATTTCGAAACCTTCACGCTGATCTATCTCAGCAGCGCCGAGACCGGCTTTGAACTGGAGC
TGACCGTCAACAAAGGCCGGAGCGAACCCTACGATCTCGGCAATGCCTACGGCCATCTCGCCATCTCCGTCGAGGAGGTG
ACCGTCGAGCGTGAACGGCTGTCGAAGCTGGGGCTGAAGCCGGGTCAGCTGGTGGAACTCAACCGCGACGGCAAACTCTT
CGGCCTGTTCTTCTTCGTCAGCGATCCCGACGGCTACAAGATCGAAGTGCTGCAGCGCCACGGCCGGTTTCTCTAG

Upstream 100 bases:

>100_bases
CCATGGGTCTTGTCGAAGCGGCGATCACCCGAAGAATCGGCTTGCGCCCGGCGTGAAAGGACCGCTACCACAGCTGTGAC
CGAAAGAATGGGAGGCAGAC

Downstream 100 bases:

>100_bases
GAATATTTGATGATCGAAAAGACCGAGCTCAATTCCGGCTGGACGCTTCACTGCAACGATACAGGAAGGCCTGGCCTGCC
GGAAACAATCCCGGCGACGG

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MAKMIHSMIRVLDEARSVEFYRKAFGLSVADRVDFETFTLIYLSSAETGFELELTVNKGRSEPYDLGNAYGHLAISVEEV
TVERERLSKLGLKPGQLVELNRDGKLFGLFFFVSDPDGYKIEVLQRHGRFL

Sequences:

>Translated_131_residues
MAKMIHSMIRVLDEARSVEFYRKAFGLSVADRVDFETFTLIYLSSAETGFELELTVNKGRSEPYDLGNAYGHLAISVEEV
TVERERLSKLGLKPGQLVELNRDGKLFGLFFFVSDPDGYKIEVLQRHGRFL
>Mature_130_residues
AKMIHSMIRVLDEARSVEFYRKAFGLSVADRVDFETFTLIYLSSAETGFELELTVNKGRSEPYDLGNAYGHLAISVEEVT
VERERLSKLGLKPGQLVELNRDGKLFGLFFFVSDPDGYKIEVLQRHGRFL

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Escherichia coli, GI1787940, Length=128, Percent_Identity=32.8125, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14938; Mature: 14807

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMIHSMIRVLDEARSVEFYRKAFGLSVADRVDFETFTLIYLSSAETGFELELTVNKGR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEECCCCCEEEEEEEECCC
SEPYDLGNAYGHLAISVEEVTVERERLSKLGLKPGQLVELNRDGKLFGLFFFVSDPDGYK
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCCCCE
IEVLQRHGRFL
EEEEECCCCCC
>Mature Secondary Structure 
AKMIHSMIRVLDEARSVEFYRKAFGLSVADRVDFETFTLIYLSSAETGFELELTVNKGR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEECCCCCEEEEEEEECCC
SEPYDLGNAYGHLAISVEEVTVERERLSKLGLKPGQLVELNRDGKLFGLFFFVSDPDGYK
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCCCCE
IEVLQRHGRFL
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]