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The map label for this gene is nemA [H]

Identifier: 209547724

GI number: 209547724

Start: 105645

End: 106739

Strand: Direct

Name: nemA [H]

Synonym: Rleg2_0113

Alternate gene names: 209547724

Gene position: 105645-106739 (Clockwise)

Preceding gene: 209547723

Following gene: 209547726

Centisome position: 2.33

GC content: 61.64

Gene sequence:

>1095_bases
ATGACCAGCAAGACACTCTTTGAACCCTTCGTACTCGGCTCTCTCACACTATCCAACCGCATCGTCATGGCGCCATTGAC
GCGCAACAGAGCGGGTGCCGGGTTTGTGCCGGGCGATCTGATTGCCGAATATTATGCTCAACGGGCCTCGGCAGGCCTGA
TCATCTCGGAGGCGACCCAGATTTCGCAGCAGGGTCAAGGCTACCAGGACACGCCCGGCATATACACGCAGGCGCAGATC
GACGGCTGGCGCAAGGTGACCGATGCGGTGCATGCCAAGGGCGGCCACATCTTTCTGCAGCTCTGGCATGTCGGTCGGGT
ATCGCATGTCGACCTACAGCCGAATGGGCAGCACCCGGTGGCGCCTTCCGCGATCAAGGCCGAAACCAAGACCTTTGTGA
ACAACAGCTTCGTCGACGTGTCGGCCCCTCGGGCGCTCGAATTGCATGAGATCTCCGGCATCGTCGAGGATTTCCGCCGG
GCCGCCGCCAATGCCATTGCCGCCGGGTTCGATGGTGTCGAAATTCACGGCGCCAACGGCTACCTGCTCGACCAGTTCGC
CAGGGACGGAGCCAACACCCGCACCGACGCCTATGGCGGCTCGATCGAAAACCGGGCCCGGCTGATGCTCGAGGTGACGG
CGGCGGTCATTAAGGAAGTGGGGGCGAAGCGTACCGGAGTTCGGATTTCACCGGTGTCGCCGGCCAACGGAATATCAGCA
ACCGATCCGCAGGCGCAGTTCAACTACATCGTCGAGAAGCTCGACGAGATGGGCATTGCCTACCTCCATGTCGTCGAAGG
TGCGACGGGCGGGCCGCGCGATGTTGCGCCCTTCGATTACGAGGCGCTTCGCCGCAGGTTCAGTAACACTTACATCGCCA
ATAACGGCTACGATCGGGAACTCGCGACGGCCAGGCTGGAGCAGGGGCTGGCGGACCTGTTTGCCTTCGGCAGGCCGTTC
ATCGCCAACCCCGATTTTGTCGAGCGTCTGAAAATCGGTGCATCACTGGCCAATCTCGATATGGCGACGCTCTATGGCGG
CGGCGCGGCGGGCTATACGGACTATCCCGAAATGGCCGAACCGGTTAGCGCTTGA

Upstream 100 bases:

>100_bases
GCAAGGTGGTCATCACGGTCGCAGACTGAGGTCGACCTGACCGACGCGGCGTGCGTTGCGAGACGAACGCGAGAAATAAT
TCCAACTCTGAAAGCGAAAA

Downstream 100 bases:

>100_bases
CGGCATAGGGCCGCCTATACGGCGGCCCTATTATCTCGGGCCCGATTTTCGATGGCGTCGCGGCAATCCGCCCGGCAGGC
GTCGAGCAGTTCGTCCGCCA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTSKTLFEPFVLGSLTLSNRIVMAPLTRNRAGAGFVPGDLIAEYYAQRASAGLIISEATQISQQGQGYQDTPGIYTQAQI
DGWRKVTDAVHAKGGHIFLQLWHVGRVSHVDLQPNGQHPVAPSAIKAETKTFVNNSFVDVSAPRALELHEISGIVEDFRR
AAANAIAAGFDGVEIHGANGYLLDQFARDGANTRTDAYGGSIENRARLMLEVTAAVIKEVGAKRTGVRISPVSPANGISA
TDPQAQFNYIVEKLDEMGIAYLHVVEGATGGPRDVAPFDYEALRRRFSNTYIANNGYDRELATARLEQGLADLFAFGRPF
IANPDFVERLKIGASLANLDMATLYGGGAAGYTDYPEMAEPVSA

Sequences:

>Translated_364_residues
MTSKTLFEPFVLGSLTLSNRIVMAPLTRNRAGAGFVPGDLIAEYYAQRASAGLIISEATQISQQGQGYQDTPGIYTQAQI
DGWRKVTDAVHAKGGHIFLQLWHVGRVSHVDLQPNGQHPVAPSAIKAETKTFVNNSFVDVSAPRALELHEISGIVEDFRR
AAANAIAAGFDGVEIHGANGYLLDQFARDGANTRTDAYGGSIENRARLMLEVTAAVIKEVGAKRTGVRISPVSPANGISA
TDPQAQFNYIVEKLDEMGIAYLHVVEGATGGPRDVAPFDYEALRRRFSNTYIANNGYDRELATARLEQGLADLFAFGRPF
IANPDFVERLKIGASLANLDMATLYGGGAAGYTDYPEMAEPVSA
>Mature_363_residues
TSKTLFEPFVLGSLTLSNRIVMAPLTRNRAGAGFVPGDLIAEYYAQRASAGLIISEATQISQQGQGYQDTPGIYTQAQID
GWRKVTDAVHAKGGHIFLQLWHVGRVSHVDLQPNGQHPVAPSAIKAETKTFVNNSFVDVSAPRALELHEISGIVEDFRRA
AANAIAAGFDGVEIHGANGYLLDQFARDGANTRTDAYGGSIENRARLMLEVTAAVIKEVGAKRTGVRISPVSPANGISAT
DPQAQFNYIVEKLDEMGIAYLHVVEGATGGPRDVAPFDYEALRRRFSNTYIANNGYDRELATARLEQGLADLFAFGRPFI
ANPDFVERLKIGASLANLDMATLYGGGAAGYTDYPEMAEPVSA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=51.2328767123288, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=30.835734870317, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17565138, Length=345, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=293, Percent_Identity=27.9863481228669, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=282, Percent_Identity=27.3049645390071, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=269, Percent_Identity=31.9702602230483, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=267, Percent_Identity=28.4644194756554, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17564188, Length=270, Percent_Identity=26.2962962962963, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17566914, Length=362, Percent_Identity=25.1381215469613, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=390, Percent_Identity=35.3846153846154, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321973, Length=387, Percent_Identity=37.2093023255814, Blast_Score=204, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39163; Mature: 39032

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKTLFEPFVLGSLTLSNRIVMAPLTRNRAGAGFVPGDLIAEYYAQRASAGLIISEATQ
CCCCHHHHHHHHHEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHH
ISQQGQGYQDTPGIYTQAQIDGWRKVTDAVHAKGGHIFLQLWHVGRVSHVDLQPNGQHPV
HHHCCCCCCCCCCCEEEHHHCHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCC
APSAIKAETKTFVNNSFVDVSAPRALELHEISGIVEDFRRAAANAIAAGFDGVEIHGANG
CCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFARDGANTRTDAYGGSIENRARLMLEVTAAVIKEVGAKRTGVRISPVSPANGISA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
TDPQAQFNYIVEKLDEMGIAYLHVVEGATGGPRDVAPFDYEALRRRFSNTYIANNGYDRE
CCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCCCHH
LATARLEQGLADLFAFGRPFIANPDFVERLKIGASLANLDMATLYGGGAAGYTDYPEMAE
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCHHHCC
PVSA
CCCC
>Mature Secondary Structure 
TSKTLFEPFVLGSLTLSNRIVMAPLTRNRAGAGFVPGDLIAEYYAQRASAGLIISEATQ
CCCHHHHHHHHHEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHH
ISQQGQGYQDTPGIYTQAQIDGWRKVTDAVHAKGGHIFLQLWHVGRVSHVDLQPNGQHPV
HHHCCCCCCCCCCCEEEHHHCHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCC
APSAIKAETKTFVNNSFVDVSAPRALELHEISGIVEDFRRAAANAIAAGFDGVEIHGANG
CCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFARDGANTRTDAYGGSIENRARLMLEVTAAVIKEVGAKRTGVRISPVSPANGISA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
TDPQAQFNYIVEKLDEMGIAYLHVVEGATGGPRDVAPFDYEALRRRFSNTYIANNGYDRE
CCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCCCHH
LATARLEQGLADLFAFGRPFIANPDFVERLKIGASLANLDMATLYGGGAAGYTDYPEMAE
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCHHHCC
PVSA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]