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The map label for this gene is gcvA [H]

Identifier: 209547704

GI number: 209547704

Start: 87910

End: 88785

Strand: Reverse

Name: gcvA [H]

Synonym: Rleg2_0093

Alternate gene names: 209547704

Gene position: 88785-87910 (Counterclockwise)

Preceding gene: 209547707

Following gene: 209547701

Centisome position: 1.96

GC content: 68.84

Gene sequence:

>876_bases
ATGGCCCAACCCCGCCGCAAGCTGCCGCCGCTGAATGCGCTGCGCGCCTTCGAAGTCTCGGGCCGCAGGTTGAATTTCCG
CGCCGCCGCCGAGGAGCTGGGGGTCTCGCAGGGTGCGGTCGCCCAGCAGGTGCGCGCGCTCGAAGATCAGCTCGGCCTGA
TGTTGTTTCAGCGCCTGCCGCGCGGCCTGGCTCTGACCCCGCATGGCGCTGCCTATCTGACGGATGTGACCCGCGCCTTC
GATACGCTCGGTGAGGCGACCGGGCGGCTTCTCGCGCGGCCGGATGCCGTGACGATCAGCGTCACCCCGACGGTCGCCGC
CAAGCTGCTGATCCCGCGCCTCGCCGAGTTCAAATCCGCCCTCCCCGACGTGGAATTGCGGACGGTCGCCACCGAGGCGC
TGTCCGATTTCGACCGCGATCAGGTGGACATTGCGGTGCGGCTGACCCGCAGGCCTTTTCCGGCCTCGCTGGAGGCAAGG
CTGCTGTTCCGCCAGGAACTGGTCGCCGTCGCCAGCCCATACCTGGTCAAGGGCCTGCCCCTTCCATTGACGGCCGACGC
GCTTCGCAAGCTGCCGCTTCTGCATGACGCCGTCAGCCCCTGGCCGCTCGTGCTGCGATCGCGCGAGAAGCTGCCGGGGG
CGGTGTTCAACCATACGACGCTGGCGCTGGATGCCGCCCTCGCCGGCCAGGGCGTCGCCTTGGCCTGCCGGGCGTTCGTG
GCCGCCGATCTCGAGGCGGGGCGGCTGGTGCAGGTGATCGATGCGACGACGCTCATCGAACCCGACTACTTCCTTATCCG
CAAACGCGCGTCATCACCGCGAAAGGCGGTCGATGCGGTATGGTATTGGTGCGCGGCGCGGCTGTCGGTCGGTTAA

Upstream 100 bases:

>100_bases
GTTGAATTGCGAAGCAGTATGCCAGAGACAGCGCCGGCTTTCGCGCCAGAAAGAAACGTGTCAGACTGTAGAAATTCTAC
TGGAATATCCGGAAATCTCA

Downstream 100 bases:

>100_bases
ACCGGCAAACCGACACCAAGTCGGATTTCAACCCTTGGGATCAGGGATCAGGCGTATCAGCGTTCCGTTACGCGCCGCGC
CGCTGATTGCCGTTGAGATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAQPRRKLPPLNALRAFEVSGRRLNFRAAAEELGVSQGAVAQQVRALEDQLGLMLFQRLPRGLALTPHGAAYLTDVTRAF
DTLGEATGRLLARPDAVTISVTPTVAAKLLIPRLAEFKSALPDVELRTVATEALSDFDRDQVDIAVRLTRRPFPASLEAR
LLFRQELVAVASPYLVKGLPLPLTADALRKLPLLHDAVSPWPLVLRSREKLPGAVFNHTTLALDAALAGQGVALACRAFV
AADLEAGRLVQVIDATTLIEPDYFLIRKRASSPRKAVDAVWYWCAARLSVG

Sequences:

>Translated_291_residues
MAQPRRKLPPLNALRAFEVSGRRLNFRAAAEELGVSQGAVAQQVRALEDQLGLMLFQRLPRGLALTPHGAAYLTDVTRAF
DTLGEATGRLLARPDAVTISVTPTVAAKLLIPRLAEFKSALPDVELRTVATEALSDFDRDQVDIAVRLTRRPFPASLEAR
LLFRQELVAVASPYLVKGLPLPLTADALRKLPLLHDAVSPWPLVLRSREKLPGAVFNHTTLALDAALAGQGVALACRAFV
AADLEAGRLVQVIDATTLIEPDYFLIRKRASSPRKAVDAVWYWCAARLSVG
>Mature_290_residues
AQPRRKLPPLNALRAFEVSGRRLNFRAAAEELGVSQGAVAQQVRALEDQLGLMLFQRLPRGLALTPHGAAYLTDVTRAFD
TLGEATGRLLARPDAVTISVTPTVAAKLLIPRLAEFKSALPDVELRTVATEALSDFDRDQVDIAVRLTRRPFPASLEARL
LFRQELVAVASPYLVKGLPLPLTADALRKLPLLHDAVSPWPLVLRSREKLPGAVFNHTTLALDAALAGQGVALACRAFVA
ADLEAGRLVQVIDATTLIEPDYFLIRKRASSPRKAVDAVWYWCAARLSVG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=37.9310344827586, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=29.6928327645051, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788706, Length=270, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI145693193, Length=259, Percent_Identity=30.1158301158301, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=29.3172690763052, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081978, Length=272, Percent_Identity=28.6764705882353, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=23.859649122807, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=284, Percent_Identity=25.7042253521127, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=179, Percent_Identity=30.7262569832402, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31647; Mature: 31516

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPRRKLPPLNALRAFEVSGRRLNFRAAAEELGVSQGAVAQQVRALEDQLGLMLFQRLP
CCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
RGLALTPHGAAYLTDVTRAFDTLGEATGRLLARPDAVTISVTPTVAAKLLIPRLAEFKSA
CCCEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHH
LPDVELRTVATEALSDFDRDQVDIAVRLTRRPFPASLEARLLFRQELVAVASPYLVKGLP
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCCC
LPLTADALRKLPLLHDAVSPWPLVLRSREKLPGAVFNHTTLALDAALAGQGVALACRAFV
CCCCHHHHHHCCHHHHCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHH
AADLEAGRLVQVIDATTLIEPDYFLIRKRASSPRKAVDAVWYWCAARLSVG
HHCCCCCCEEEEHHHHHEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQPRRKLPPLNALRAFEVSGRRLNFRAAAEELGVSQGAVAQQVRALEDQLGLMLFQRLP
CCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
RGLALTPHGAAYLTDVTRAFDTLGEATGRLLARPDAVTISVTPTVAAKLLIPRLAEFKSA
CCCEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHH
LPDVELRTVATEALSDFDRDQVDIAVRLTRRPFPASLEARLLFRQELVAVASPYLVKGLP
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCCC
LPLTADALRKLPLLHDAVSPWPLVLRSREKLPGAVFNHTTLALDAALAGQGVALACRAFV
CCCCHHHHHHCCHHHHCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHH
AADLEAGRLVQVIDATTLIEPDYFLIRKRASSPRKAVDAVWYWCAARLSVG
HHCCCCCCEEEEHHHHHEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]