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The map label for this gene is rpoN [H]

Identifier: 209547660

GI number: 209547660

Start: 48393

End: 49955

Strand: Direct

Name: rpoN [H]

Synonym: Rleg2_0048

Alternate gene names: 209547660

Gene position: 48393-49955 (Clockwise)

Preceding gene: 209547659

Following gene: 209547661

Centisome position: 1.07

GC content: 63.34

Gene sequence:

>1563_bases
ATGGCACTGTCCGCCAGTCTTTTCCTGCGCCAGAACCAATCGCTGGTGATGACACCGCAGCTGATGCAATCCATCCAGCT
GCTGCAGATGACCCATGTCGAACTCACTCAGTTCATCGCCCAGGAAGTGGAGAAGAACCCGCTGCTCGAATTTCCGTCGA
ATGACGGCGAGGCGGGCGCAGAACGCGGCGAATCCGAGGACGAGCCCTATGGTCATTCCCCGGAGGATGCGGGCTCGGAC
GACGGCTACGACAACCGCACCGACGCGCTCTCCAGCGACTGGTACGACAATGGCGGCAGCGCCAGCACCAGCCGGCTGAA
CGACGAGCTCGACGCCAACTACACCAACGTCTATCCTGACGACAGCGCGCCGCAGCGCCTCGATGCGCCCGAACTCGTCG
GCCAGTGGAAATCGATGCCGGGCAGCGGCGGCGAGGGCGCCGATTACGATCTCGACGATTTCGTCGCCGGCCAGGTGTCG
CTGCGCGATCATCTCGCCCAGCAGATCCCCTTCGTCCTGCCCGACATGGCCGATCGGCTGATTGCCCAGAATTTCGTCGA
TCAGCTCGACGATGCCGGTTATCTGCAAGTCGATCTCGTCGAGACCGGCGAGCGGCTGGGCACGAGCCTGACTGCGGCCG
AGCGTGTGCTTGCCGCCCTCCAGACGCTCGATCCGCCCGGGGTTTTCGCCCGCAGCCTCTCGGAATGCCTGGCGATCCAG
CTCAGGCAGAAGGACCGGTACGACCCCGCCATGCAGGCGCTGGTCGAAAATCTCGAACTGCTGGCGCGGCGCGATTTTGC
CACGCTGAAGCGGCTCTGCGGCGTCGACGAGGAAGACCTTCTCGATATGCTCGGCGAAATCCGGCAGCTCAATCCCAAGC
CCGGCAGCGGTTTCGAGACCGGCGTTTCCGAGGCGATCATGCCCGATGTGGTCGTCCGGCCTTCCCTCGATGGCGGCTGG
CTGGTCGAGCTCAATCCGGATACGCTGCCGCGCGTGCTCGTCAATCAGTCCTATTTTTCCCGGGTGACGAGGAATGGCGA
CGATCACGCCTTCCTTTCCGAATGCTTGCAGAGCGCCAACTGGCTGACGCGCAGCCTCGACCAGCGGGCAAAAACCATCC
TGAAGGTGGCAAGCGAGATCGTCCGCCAGCAGGATGCCTTCCTGCTGAATGGCGTCGACCACCTGCGCCCGCTGAACCTG
AAGACGGTCGCCGAGGCGATCAAGATGCACGAATCCACCGTCAGCCGCGTCACCTCGAACAAATACATGCTGACGCCGCG
CGGCCTCTTCGAGCTCAAATATTTCTTCACCGTCTCGATCAGCGCGGTTGCCGGCGGCGACAGCCATTCGGCCGAGGCGG
TGCGTCACAAGATCCGCGCGCTGATCATGCAGGAGAGCCCTGACGCGGTGCTTTCCGACGACGATATCGTCGACATGCTG
AAGAAGGGCGGTGTGGATCTCGCCCGCCGCACGGTTGCCAAATACCGCGAGGCTATGAACATCGCCTCCTCAGTCCAGCG
GCGGCGCGAAAAACGCGCGCTCGCCAAGGTCGCCGGTTTCTGA

Upstream 100 bases:

>100_bases
ACAACAAAAGAGCAATTTTTGGGCCAAGTTGGATTTCCGTGGCCTGAAGCCTTGATCGGACGCGGTTTCCCGGAGGAATT
GAGGGAGTTTCGCGTCCGTC

Downstream 100 bases:

>100_bases
CGCCGCAATGCTTCGGTCTTGATCGAAGCATCCAGCTCCCAATCCCCGTTATCTCTCCGACGTCTTCAACGAACTGGGGA
AAGCCGACCATGACGCCGCA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MALSASLFLRQNQSLVMTPQLMQSIQLLQMTHVELTQFIAQEVEKNPLLEFPSNDGEAGAERGESEDEPYGHSPEDAGSD
DGYDNRTDALSSDWYDNGGSASTSRLNDELDANYTNVYPDDSAPQRLDAPELVGQWKSMPGSGGEGADYDLDDFVAGQVS
LRDHLAQQIPFVLPDMADRLIAQNFVDQLDDAGYLQVDLVETGERLGTSLTAAERVLAALQTLDPPGVFARSLSECLAIQ
LRQKDRYDPAMQALVENLELLARRDFATLKRLCGVDEEDLLDMLGEIRQLNPKPGSGFETGVSEAIMPDVVVRPSLDGGW
LVELNPDTLPRVLVNQSYFSRVTRNGDDHAFLSECLQSANWLTRSLDQRAKTILKVASEIVRQQDAFLLNGVDHLRPLNL
KTVAEAIKMHESTVSRVTSNKYMLTPRGLFELKYFFTVSISAVAGGDSHSAEAVRHKIRALIMQESPDAVLSDDDIVDML
KKGGVDLARRTVAKYREAMNIASSVQRRREKRALAKVAGF

Sequences:

>Translated_520_residues
MALSASLFLRQNQSLVMTPQLMQSIQLLQMTHVELTQFIAQEVEKNPLLEFPSNDGEAGAERGESEDEPYGHSPEDAGSD
DGYDNRTDALSSDWYDNGGSASTSRLNDELDANYTNVYPDDSAPQRLDAPELVGQWKSMPGSGGEGADYDLDDFVAGQVS
LRDHLAQQIPFVLPDMADRLIAQNFVDQLDDAGYLQVDLVETGERLGTSLTAAERVLAALQTLDPPGVFARSLSECLAIQ
LRQKDRYDPAMQALVENLELLARRDFATLKRLCGVDEEDLLDMLGEIRQLNPKPGSGFETGVSEAIMPDVVVRPSLDGGW
LVELNPDTLPRVLVNQSYFSRVTRNGDDHAFLSECLQSANWLTRSLDQRAKTILKVASEIVRQQDAFLLNGVDHLRPLNL
KTVAEAIKMHESTVSRVTSNKYMLTPRGLFELKYFFTVSISAVAGGDSHSAEAVRHKIRALIMQESPDAVLSDDDIVDML
KKGGVDLARRTVAKYREAMNIASSVQRRREKRALAKVAGF
>Mature_519_residues
ALSASLFLRQNQSLVMTPQLMQSIQLLQMTHVELTQFIAQEVEKNPLLEFPSNDGEAGAERGESEDEPYGHSPEDAGSDD
GYDNRTDALSSDWYDNGGSASTSRLNDELDANYTNVYPDDSAPQRLDAPELVGQWKSMPGSGGEGADYDLDDFVAGQVSL
RDHLAQQIPFVLPDMADRLIAQNFVDQLDDAGYLQVDLVETGERLGTSLTAAERVLAALQTLDPPGVFARSLSECLAIQL
RQKDRYDPAMQALVENLELLARRDFATLKRLCGVDEEDLLDMLGEIRQLNPKPGSGFETGVSEAIMPDVVVRPSLDGGWL
VELNPDTLPRVLVNQSYFSRVTRNGDDHAFLSECLQSANWLTRSLDQRAKTILKVASEIVRQQDAFLLNGVDHLRPLNLK
TVAEAIKMHESTVSRVTSNKYMLTPRGLFELKYFFTVSISAVAGGDSHSAEAVRHKIRALIMQESPDAVLSDDDIVDMLK
KGGVDLARRTVAKYREAMNIASSVQRRREKRALAKVAGF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes (nif operon), glnA and dctA for dicar

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=519, Percent_Identity=36.0308285163777, Blast_Score=301, Evalue=5e-83,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 57462; Mature: 57331

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSASLFLRQNQSLVMTPQLMQSIQLLQMTHVELTQFIAQEVEKNPLLEFPSNDGEAGA
CCCCHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
ERGESEDEPYGHSPEDAGSDDGYDNRTDALSSDWYDNGGSASTSRLNDELDANYTNVYPD
HCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCC
DSAPQRLDAPELVGQWKSMPGSGGEGADYDLDDFVAGQVSLRDHLAQQIPFVLPDMADRL
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IAQNFVDQLDDAGYLQVDLVETGERLGTSLTAAERVLAALQTLDPPGVFARSLSECLAIQ
HHHHHHHHHCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRQKDRYDPAMQALVENLELLARRDFATLKRLCGVDEEDLLDMLGEIRQLNPKPGSGFET
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHH
GVSEAIMPDVVVRPSLDGGWLVELNPDTLPRVLVNQSYFSRVTRNGDDHAFLSECLQSAN
HHHHHHCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
WLTRSLDQRAKTILKVASEIVRQQDAFLLNGVDHLRPLNLKTVAEAIKMHESTVSRVTSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
KYMLTPRGLFELKYFFTVSISAVAGGDSHSAEAVRHKIRALIMQESPDAVLSDDDIVDML
CEEECCCCHHHEEEEHEEEEEHEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KKGGVDLARRTVAKYREAMNIASSVQRRREKRALAKVAGF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALSASLFLRQNQSLVMTPQLMQSIQLLQMTHVELTQFIAQEVEKNPLLEFPSNDGEAGA
CCCHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
ERGESEDEPYGHSPEDAGSDDGYDNRTDALSSDWYDNGGSASTSRLNDELDANYTNVYPD
HCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCC
DSAPQRLDAPELVGQWKSMPGSGGEGADYDLDDFVAGQVSLRDHLAQQIPFVLPDMADRL
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IAQNFVDQLDDAGYLQVDLVETGERLGTSLTAAERVLAALQTLDPPGVFARSLSECLAIQ
HHHHHHHHHCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRQKDRYDPAMQALVENLELLARRDFATLKRLCGVDEEDLLDMLGEIRQLNPKPGSGFET
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHH
GVSEAIMPDVVVRPSLDGGWLVELNPDTLPRVLVNQSYFSRVTRNGDDHAFLSECLQSAN
HHHHHHCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
WLTRSLDQRAKTILKVASEIVRQQDAFLLNGVDHLRPLNLKTVAEAIKMHESTVSRVTSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
KYMLTPRGLFELKYFFTVSISAVAGGDSHSAEAVRHKIRALIMQESPDAVLSDDDIVDML
CEEECCCCHHHEEEEHEEEEEHEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KKGGVDLARRTVAKYREAMNIASSVQRRREKRALAKVAGF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537369 [H]