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The map label for this gene is ysgA [H]

Identifier: 209547650

GI number: 209547650

Start: 40759

End: 41619

Strand: Reverse

Name: ysgA [H]

Synonym: Rleg2_0038

Alternate gene names: 209547650

Gene position: 41619-40759 (Counterclockwise)

Preceding gene: 209547651

Following gene: 209547649

Centisome position: 0.92

GC content: 65.51

Gene sequence:

>861_bases
ATGAGCAAGGACTTCCACGATCAGGGACCGCGCAGGGTCGGACAGGTGAAGGAAGTCACCTCGCTGGCCAATCCGATCAT
CAAGGACATCAAGGCGCTGACGAACAAGAAGTCGCGGGAGGAAAGCGGAACATTCCTCGCCGAAGGACTGAAGCTCGTCA
TCGACGCGATCGAGCTCGGCTGGACGATCCGCACGCTGGTCTATGCCAAAGCCGCCAAGGGCAAGCCGCTGGTCGAGCAG
ATGGCGGCAAGGACCGTCGCCTCGGGCGGGCTGGTGCTCGAAGTCAGCGAAAAGGTGATCGCCTCGATCACCCGGCGCGA
CAATCCGCAGATGGTCGTCGGCATCTTCGAGCAGCGCTGGATGCCGCTGCGGGACATTCGGCCGAGGGATGGCGAGACCT
GGGTGGCGCTCGACCGGGTGCGCGACCCCGGCAATCTCGGCACCATCATCCGCACAGCCGATGCGGCCGGCGCTTCCGGC
ATCATCCTGGTCGGAGAGACGACCGATCCCTTTTCGCTCGAGACGGTGCGGGCCACCATGGGTTCGGTCTTTGCCGTTCC
GGTGGCGCGGGCAGCGCCGGAGGAGTTCATCGCCTGGCGGAAATCGGCCGGCGTTTCCGTGGTCGCGACGCATCTTGCCG
GCGCGGTCGATTACCGCACCATCGACTACCGGAAAAAGCCCGTCGTGCTGCTGATGGGCAATGAGCAATCCGGCCTGCCG
GACCAGCTGGCCGGGCAAGCCGACGCGCTTGCCCGCATTCCCCAGCAGGGCCGCGCCGATTCCCTGAACCTCGCCGTCGC
CACCGCCGTCATGCTTTTCGAGGCGCGCCGCCATCTCCTCTCACTTGCCGAGGGCAAATGA

Upstream 100 bases:

>100_bases
TGGTCGAATCCGGCGAGCTGGTCATCCGCGAAGCCGGCCTCGACGGCAAGACGCCGGGCCGCGCGCTTTCCACCTCCCTC
TTTTCCCGCTGGGTGCCGAA

Downstream 100 bases:

>100_bases
CCGAACTGACGCCTGCCCGCCCCGCGCTGTTTTCGCGCCCCGCGCCCATCCTCTTCTTCATCATCGTCGCCGTTCTCCTC
GACCAAATCGTCAAGATCGC

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSKDFHDQGPRRVGQVKEVTSLANPIIKDIKALTNKKSREESGTFLAEGLKLVIDAIELGWTIRTLVYAKAAKGKPLVEQ
MAARTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQRWMPLRDIRPRDGETWVALDRVRDPGNLGTIIRTADAAGASG
IILVGETTDPFSLETVRATMGSVFAVPVARAAPEEFIAWRKSAGVSVVATHLAGAVDYRTIDYRKKPVVLLMGNEQSGLP
DQLAGQADALARIPQQGRADSLNLAVATAVMLFEARRHLLSLAEGK

Sequences:

>Translated_286_residues
MSKDFHDQGPRRVGQVKEVTSLANPIIKDIKALTNKKSREESGTFLAEGLKLVIDAIELGWTIRTLVYAKAAKGKPLVEQ
MAARTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQRWMPLRDIRPRDGETWVALDRVRDPGNLGTIIRTADAAGASG
IILVGETTDPFSLETVRATMGSVFAVPVARAAPEEFIAWRKSAGVSVVATHLAGAVDYRTIDYRKKPVVLLMGNEQSGLP
DQLAGQADALARIPQQGRADSLNLAVATAVMLFEARRHLLSLAEGK
>Mature_285_residues
SKDFHDQGPRRVGQVKEVTSLANPIIKDIKALTNKKSREESGTFLAEGLKLVIDAIELGWTIRTLVYAKAAKGKPLVEQM
AARTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQRWMPLRDIRPRDGETWVALDRVRDPGNLGTIIRTADAAGASGI
ILVGETTDPFSLETVRATMGSVFAVPVARAAPEEFIAWRKSAGVSVVATHLAGAVDYRTIDYRKKPVVLLMGNEQSGLPD
QLAGQADALARIPQQGRADSLNLAVATAVMLFEARRHLLSLAEGK

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=144, Percent_Identity=36.1111111111111, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=31.3725490196078, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666840, Length=282, Percent_Identity=23.4042553191489, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 30944; Mature: 30813

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDFHDQGPRRVGQVKEVTSLANPIIKDIKALTNKKSREESGTFLAEGLKLVIDAIELG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHC
WTIRTLVYAKAAKGKPLVEQMAARTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQRW
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCEEEEEHHHCC
MPLRDIRPRDGETWVALDRVRDPGNLGTIIRTADAAGASGIILVGETTDPFSLETVRATM
CCHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
GSVFAVPVARAAPEEFIAWRKSAGVSVVATHLAGAVDYRTIDYRKKPVVLLMGNEQSGLP
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCEEEECCCCCCEEEEECCCCCCCC
DQLAGQADALARIPQQGRADSLNLAVATAVMLFEARRHLLSLAEGK
HHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKDFHDQGPRRVGQVKEVTSLANPIIKDIKALTNKKSREESGTFLAEGLKLVIDAIELG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHC
WTIRTLVYAKAAKGKPLVEQMAARTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQRW
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCEEEEEHHHCC
MPLRDIRPRDGETWVALDRVRDPGNLGTIIRTADAAGASGIILVGETTDPFSLETVRATM
CCHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
GSVFAVPVARAAPEEFIAWRKSAGVSVVATHLAGAVDYRTIDYRKKPVVLLMGNEQSGLP
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCEEEECCCCCCEEEEECCCCCCCC
DQLAGQADALARIPQQGRADSLNLAVATAVMLFEARRHLLSLAEGK
HHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]