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The map label for this gene is dnaA [H]

Identifier: 209547613

GI number: 209547613

Start: 620

End: 2170

Strand: Direct

Name: dnaA [H]

Synonym: Rleg2_0001

Alternate gene names: 209547613

Gene position: 620-2170 (Clockwise)

Preceding gene: NA

Following gene: 209547614

Centisome position: 0.01

GC content: 63.96

Gene sequence:

>1551_bases
ATGCAGATGAATACGATGACGGCGGGCGGGCCCGACAATGGGGATGTGGCACCGCAGGCGTTTGGCTCCATTCGCCTCGA
AGGGGGAGACACAAAGGCGGAAATGAAGCACAGCATCCTGTTTGAGCGCGTCAGCGCGCGCTTGAAGGCCCAGGTCGGCC
CTGACGTCTATGCCAGCTGGTTCGCCCGGCTGAAGCTGCATTCGGTATCGAAGAGCGTGGTTCGCCTGTCGGTTCCCACC
ACCTTCCTGAAGTCGTGGATCAACAATCGTTATCTCGATCTCATCACCGGTCTGTTCCAGGCCGAAGATCCGGAAATTAT
GAAGATCGAAGTTCTGGTGCGCACAGCGACGCGCAGCGGCACGAAGGCGCTCGATGAGGCAGTCCAGCCCGAGCCGGTCG
TTGCCCCCCAGGCGCGCCGCCCCGCAAGCGCCCAGCCGGCCGGCCAGGCCGTCCAGCAGGCGGTTTCTTCCGTTGCTGCG
GCAAGGCCCGCAAGCTTTGGTTCGCCGCTCTTCGGTTCGCCGCTCGACAGCCGCTTCACTTTCGACACCTTCGTCGAAGG
CAGCTCGAACCGCGTCGCACTTGCGGCCGCCAAGACGATCGCCGAAGCCGGTTCCGGCGCCGTGCGCTTCAATCCGCTGT
TCATCCATTCGACGGTCGGCCTCGGCAAGACCCACCTGCTGCAGGCAATCGCCAATGCGGCGGTGCAGAACCCGCGGGCG
CTGCGCGTCGTCTATCTGACGGCCGAGTATTTCATGTGGCGCTTCGCCACCGCGATCCGCGACAATGATGCGCTGACGCT
GAAGGATTCGCTGCGCAACATCGATCTGTTGATCATCGACGACATGCAGTTCCTGCAGGGCAAGATGATCCAGCACGAAT
TCTGCCATCTCCTCAACATGCTGCTCGACAGCGCCAAGCAGGTCGTCGTCGCCGCCGACCGCGCCCCCTGGGAGCTGGAA
TCGCTCGACCCGCGTGTACGCTCGCGCCTGCAGGGCGGTGTCGCGATCGAATTCGACGCGCCGGATTATGAGATGCGCCT
CGAGATCCTCCGGCGTCGCCTTGCTGCGGCCCGGCTTGAAGATCCGTCGCTCGACATTCCGGCCGAACTGCTCCAGCACG
TCGCCCGCAACGTCACGGCCAGCGGCCGCGAGCTGGAAGGTGCTTTCAACCAGCTGGTCTTCCGCCGCTCCTTCGAGCCG
AACCTGTCGATCGAGCGCGTCGACGAACTGCTCGCCCATCTCGTCGGCTCCGGCGAACCCCGCCGCGTCCGCATCGAGGA
TATCCAGCGCATCGTCGCCAGGCACTACAACGTCTCGCGCCAGGAACTGGTCTCGAACCGCCGCACCCGTGTCATCGTCA
AGCCGCGCCAGATCGCCATGTATCTGTCGAAGACGCTGACGCCGCGCTCCTTCCCCGAGATCGGCCGCCGTTTCGGCGGG
CGCGATCACACGACCGTGCTGCACGCCGTGCGCAAGATCGAAGAGCTGATTTCCGGCGACACCAAGCTGTCGCACGAAGT
CGAGCTTCTGAAGCGGCTGATCAACGAATAA

Upstream 100 bases:

>100_bases
GGCAAGCGGCTTCATGAGGATGCCGTCAGTCCGCCAAAGCGGGGAATAATCGGGCGGCTATTTTAGCGGAAGCCGCCCGG
TGGAATTGAAAGGCGGCATT

Downstream 100 bases:

>100_bases
CGACCCGATTTTCGGAAAAACTTTGACGGCCGGAAGCGATTCCGGCCGTTTTGCTTTATGAGCTTTTTATTCTATACCTC
CGGCCATCCCGTGCATGCCC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MQMNTMTAGGPDNGDVAPQAFGSIRLEGGDTKAEMKHSILFERVSARLKAQVGPDVYASWFARLKLHSVSKSVVRLSVPT
TFLKSWINNRYLDLITGLFQAEDPEIMKIEVLVRTATRSGTKALDEAVQPEPVVAPQARRPASAQPAGQAVQQAVSSVAA
ARPASFGSPLFGSPLDSRFTFDTFVEGSSNRVALAAAKTIAEAGSGAVRFNPLFIHSTVGLGKTHLLQAIANAAVQNPRA
LRVVYLTAEYFMWRFATAIRDNDALTLKDSLRNIDLLIIDDMQFLQGKMIQHEFCHLLNMLLDSAKQVVVAADRAPWELE
SLDPRVRSRLQGGVAIEFDAPDYEMRLEILRRRLAAARLEDPSLDIPAELLQHVARNVTASGRELEGAFNQLVFRRSFEP
NLSIERVDELLAHLVGSGEPRRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGG
RDHTTVLHAVRKIEELISGDTKLSHEVELLKRLINE

Sequences:

>Translated_516_residues
MQMNTMTAGGPDNGDVAPQAFGSIRLEGGDTKAEMKHSILFERVSARLKAQVGPDVYASWFARLKLHSVSKSVVRLSVPT
TFLKSWINNRYLDLITGLFQAEDPEIMKIEVLVRTATRSGTKALDEAVQPEPVVAPQARRPASAQPAGQAVQQAVSSVAA
ARPASFGSPLFGSPLDSRFTFDTFVEGSSNRVALAAAKTIAEAGSGAVRFNPLFIHSTVGLGKTHLLQAIANAAVQNPRA
LRVVYLTAEYFMWRFATAIRDNDALTLKDSLRNIDLLIIDDMQFLQGKMIQHEFCHLLNMLLDSAKQVVVAADRAPWELE
SLDPRVRSRLQGGVAIEFDAPDYEMRLEILRRRLAAARLEDPSLDIPAELLQHVARNVTASGRELEGAFNQLVFRRSFEP
NLSIERVDELLAHLVGSGEPRRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGG
RDHTTVLHAVRKIEELISGDTKLSHEVELLKRLINE
>Mature_516_residues
MQMNTMTAGGPDNGDVAPQAFGSIRLEGGDTKAEMKHSILFERVSARLKAQVGPDVYASWFARLKLHSVSKSVVRLSVPT
TFLKSWINNRYLDLITGLFQAEDPEIMKIEVLVRTATRSGTKALDEAVQPEPVVAPQARRPASAQPAGQAVQQAVSSVAA
ARPASFGSPLFGSPLDSRFTFDTFVEGSSNRVALAAAKTIAEAGSGAVRFNPLFIHSTVGLGKTHLLQAIANAAVQNPRA
LRVVYLTAEYFMWRFATAIRDNDALTLKDSLRNIDLLIIDDMQFLQGKMIQHEFCHLLNMLLDSAKQVVVAADRAPWELE
SLDPRVRSRLQGGVAIEFDAPDYEMRLEILRRRLAAARLEDPSLDIPAELLQHVARNVTASGRELEGAFNQLVFRRSFEP
NLSIERVDELLAHLVGSGEPRRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGG
RDHTTVLHAVRKIEELISGDTKLSHEVELLKRLINE

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=463, Percent_Identity=35.2051835853132, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 57431; Mature: 57431

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMNTMTAGGPDNGDVAPQAFGSIRLEGGDTKAEMKHSILFERVSARLKAQVGPDVYASW
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FARLKLHSVSKSVVRLSVPTTFLKSWINNRYLDLITGLFQAEDPEIMKIEVLVRTATRSG
HHHHHHHHHHHHHEEEECCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCC
TKALDEAVQPEPVVAPQARRPASAQPAGQAVQQAVSSVAAARPASFGSPLFGSPLDSRFT
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FDTFVEGSSNRVALAAAKTIAEAGSGAVRFNPLFIHSTVGLGKTHLLQAIANAAVQNPRA
HHHHCCCCCCEEEHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
LRVVYLTAEYFMWRFATAIRDNDALTLKDSLRNIDLLIIDDMQFLQGKMIQHEFCHLLNM
EEEEEEHHHHHHHHHHHHHCCCCCEEEHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHH
LLDSAKQVVVAADRAPWELESLDPRVRSRLQGGVAIEFDAPDYEMRLEILRRRLAAARLE
HHHCCHHEEEEECCCCCCHHCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
DPSLDIPAELLQHVARNVTASGRELEGAFNQLVFRRSFEPNLSIERVDELLAHLVGSGEP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
RRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGG
CEEEHHHHHHHHHHHCCCCHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHCCC
RDHTTVLHAVRKIEELISGDTKLSHEVELLKRLINE
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQMNTMTAGGPDNGDVAPQAFGSIRLEGGDTKAEMKHSILFERVSARLKAQVGPDVYASW
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FARLKLHSVSKSVVRLSVPTTFLKSWINNRYLDLITGLFQAEDPEIMKIEVLVRTATRSG
HHHHHHHHHHHHHEEEECCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCC
TKALDEAVQPEPVVAPQARRPASAQPAGQAVQQAVSSVAAARPASFGSPLFGSPLDSRFT
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FDTFVEGSSNRVALAAAKTIAEAGSGAVRFNPLFIHSTVGLGKTHLLQAIANAAVQNPRA
HHHHCCCCCCEEEHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
LRVVYLTAEYFMWRFATAIRDNDALTLKDSLRNIDLLIIDDMQFLQGKMIQHEFCHLLNM
EEEEEEHHHHHHHHHHHHHCCCCCEEEHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHH
LLDSAKQVVVAADRAPWELESLDPRVRSRLQGGVAIEFDAPDYEMRLEILRRRLAAARLE
HHHCCHHEEEEECCCCCCHHCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
DPSLDIPAELLQHVARNVTASGRELEGAFNQLVFRRSFEPNLSIERVDELLAHLVGSGEP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
RRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLSKTLTPRSFPEIGRRFGG
CEEEHHHHHHHHHHHCCCCHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHCCC
RDHTTVLHAVRKIEELISGDTKLSHEVELLKRLINE
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA