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The map label for this gene is phnA [H]

Identifier: 209401005

GI number: 209401005

Start: 5256047

End: 5256460

Strand: Reverse

Name: phnA [H]

Synonym: ECH74115_5621

Alternate gene names: 209401005

Gene position: 5256460-5256047 (Counterclockwise)

Preceding gene: 209398018

Following gene: 209399266

Centisome position: 94.34

GC content: 46.14

Gene sequence:

>414_bases
TTGTGTATAATTGCGGCCTTTTTCGGCAATCTGCCGTTTTTTGGCGCTTTTGCCCTGCTGACTTTTGAGGAAATCCACAT
GTCATTACCACACTGCCCAAAATGCAACTCCGAATACACTTACGAAGATAACGGCATGTACATCTGCCCGGAATGTGCCT
ACGAATGGAACGACGCAGAACCTGCACAGGAAAGCGACGAGCTGATCGTTAAAGATGCTAACGGCAATCTGCTGGCTGAC
GGCGACAGCGTTACCATCATTAAAGATCTGAAGGTGAAAGGTAGCTCTTCGATGCTGAAAATTGGCACCAAAGTGAAAAA
CATCCGCCTGGTTGAAGGCGACCATAACATCGATTGCAAAATCGACGGTTTTGGTCCGATGAAACTGAAATCTGAGTTTG
TGAAAAAGAACTGA

Upstream 100 bases:

>100_bases
AATAAATGGAGGCAAAAAGATTATTTTCTTTTTGCGTTTCCTTTCAAATGAAAACGATCGTCGTCTAAAATCAGCAGTAC
CCCCGACAAACTCAGGGATT

Downstream 100 bases:

>100_bases
TTGTATTGTGATCGGTAAGCCGGATAAGGCGTTTACGCCGCATCCGGCAACAGTGCCAGATGCCTGATGCGACGCTCGCG
CGTCTTATCAGGCCTACAAA

Product: alkylphosphonate utilization operon protein PhnA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MCIIAAFFGNLPFFGAFALLTFEEIHMSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIVKDANGNLLAD
GDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMKLKSEFVKKN

Sequences:

>Translated_137_residues
MCIIAAFFGNLPFFGAFALLTFEEIHMSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIVKDANGNLLAD
GDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMKLKSEFVKKN
>Mature_137_residues
MCIIAAFFGNLPFFGAFALLTFEEIHMSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIVKDANGNLLAD
GDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMKLKSEFVKKN

Specific function: Unknown

COG id: COG2824

COG function: function code P; Uncharacterized Zn-ribbon-containing protein involved in phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790547, Length=111, Percent_Identity=100, Blast_Score=230, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004624
- InterPro:   IPR013988
- InterPro:   IPR013987 [H]

Pfam domain/function: PF03831 PhnA; PF08274 PhnA_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 15248; Mature: 15248

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCIIAAFFGNLPFFGAFALLTFEEIHMSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAE
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCC
PAQESDELIVKDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCK
CCCCCCCEEEEECCCCEEECCCCEEEEEEEEECCCCCEEEECCEEEEEEEEECCCCEEEE
IDGFGPMKLKSEFVKKN
ECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MCIIAAFFGNLPFFGAFALLTFEEIHMSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAE
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCC
PAQESDELIVKDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCK
CCCCCCCEEEEECCCCEEECCCCEEEEEEEEECCCCCEEEECCEEEEEEEEECCCCEEEE
IDGFGPMKLKSEFVKKN
ECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]