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The map label for this gene is cybB

Identifier: 209400941

GI number: 209400941

Start: 1923001

End: 1923531

Strand: Direct

Name: cybB

Synonym: ECH74115_2023

Alternate gene names: 209400941

Gene position: 1923001-1923531 (Clockwise)

Preceding gene: 209396361

Following gene: 209399017

Centisome position: 34.51

GC content: 47.83

Gene sequence:

>531_bases
ATGGAAAATAAATACTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTGCTGGTTATCGCAGCGTATTGCGCAAT
GGAGTTTCGGGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGCGGCATATCAATTCTCG
TGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACG
GGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAA
CCGAGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAA
AGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGTTTGCACGCTGCGGCTGCACTGGCACACCACTAT
TTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGA

Upstream 100 bases:

>100_bases
AGTGAAACGTAACGTACTGAAAAAGGGTGAACAATATTTAATGAAATTTTGAGAAAAGCCCGTTATGTTAACGGAAAATT
ATGTTAAAGCAGGAAATGTT

Downstream 100 bases:

>100_bases
AGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTA
TTATATTTATTTGTATGACA

Product: cytochrome b561

Products: reduced cytochrome b [C]

Alternate protein names: Cytochrome b-561

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMT
GLAHLGHLVIYLLFIALPVIGLVMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
FWKDNTLLRMMPRKRS

Sequences:

>Translated_176_residues
MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMT
GLAHLGHLVIYLLFIALPVIGLVMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
FWKDNTLLRMMPRKRS
>Mature_176_residues
MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMT
GLAHLGHLVIYLLFIALPVIGLVMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
FWKDNTLLRMMPRKRS

Specific function: B-type di-heme cytochrome with a major alpha-absorption peak at 561 nm and a minor peak at 555 nm

COG id: COG3038

COG function: function code C; Cytochrome B561

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome b561 family

Homologues:

Organism=Escherichia coli, GI87081903, Length=176, Percent_Identity=100, Blast_Score=359, Evalue=1e-101,
Organism=Escherichia coli, GI87082013, Length=175, Percent_Identity=42.2857142857143, Blast_Score=146, Evalue=7e-37,
Organism=Escherichia coli, GI1787296, Length=182, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): C561_ECOLI (P0ABE5)

Other databases:

- EMBL:   X07569
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_002041.1
- RefSeq:   NP_415935.2
- ProteinModelPortal:   P0ABE5
- STRING:   P0ABE5
- EnsemblBacteria:   EBESCT00000000036
- EnsemblBacteria:   EBESCT00000015015
- GeneID:   947241
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5224
- KEGG:   eco:b1418
- EchoBASE:   EB0169
- EcoGene:   EG10172
- eggNOG:   COG3038
- GeneTree:   EBGT00050000009431
- HOGENOM:   HBG741588
- OMA:   IKGWHER
- ProtClustDB:   PRK11513
- BioCyc:   EcoCyc:CYTOCHROME-B561-MONOMER
- Genevestigator:   P0ABE5
- GO:   GO:0006810
- InterPro:   IPR005797
- InterPro:   IPR016174

Pfam domain/function: PF00033 Cytochrom_B_N; SSF81342 Transmembr_di-haem_cytochrome

EC number: NA

Molecular weight: Translated: 20287; Mature: 20287

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x207d4a0c)-; HASH(0x1ef14250)-; HASH(0x1fbe2330)-; HASH(0x1f7de0a4)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISILVLMVVRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGLVMMYNRGNPWFAFGLTMP
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
YASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHYFWKDNTLLRMMPRKRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCH
>Mature Secondary Structure
MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISILVLMVVRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGLVMMYNRGNPWFAFGLTMP
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
YASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHYFWKDNTLLRMMPRKRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytochrome b oxidized; Electron [C]

Specific reaction: cytochrome b oxidized + 2 Electron = reduced cytochrome b [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2836696; 9097039; 9278503; 3510204